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General Information
Symbol
Dmel\chicoflp147E
Species
D. melanogaster
Name
FlyBase ID
FBal0057437
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
chico2
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Cytology
Nature of the lesion
Statement
Reference

This is an imprecise excision of the P-element from chico1. The translation start site and regulatory region are removed.

Breaks in the chico transcription unit, removing the 5' portion of the transcription unit.

Caused by aberration
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Homozygous and chico1/chicoflp147E adults show normal odour and electric shock reactivity.

Homozygous and chico1/chicoflp147E adults show significant impairment in 3 minute, 1 hour and 3 hour memory after olfactory aversive conditioning.

Compared to wild-type, chicoflp147E/chico1 mutants are reduced in weight.

chico1/chicoflp147E flies have elevated levels of lipids compared to control flies.

Homozygous pupae are more sensitive to X rays than wild type or heterozygous sibling controls.

chico1/chicoflp147E flies show lower body weight, fewer ommatidia, smaller wings, fewer cells per wing and smaller wing cell area than wild type.

Mosaic animals in which the eye is largely homozygous for chicoflp147E (generated using the "eyFLP" system) have small eyes.

The diameters of the dorsal trunk and transverse connective branches of the tracheal system are unchanged from wild type in Df(2L)flp147E larvae, while their lengths are reduced by 27% compared to wild type.

Homozygous chicoflp147E mutants (which are homozygous for Df(2L)flp147E - this causes the alleles chicoflp147E and bskflp147E, and also carrying the bsk+t5 rescue construct on the same chromosome to rescue the bskflp147E mutation) have a drastic weight reduction (65% in females, 55% in males) compared to wild-type. Body size reduction is observed at all developmental stages but does not alter the overall proportions of the flies. This is caused by a reduction in both cell size and number. In the wing, reduction in cell number accounts for 68% of the total reduction in wing size; the remaining 32% is due to the reduction in the average size of mutant cells. In homozygotes the ommatidial number is reduced by approximately 40%, from about 780 to about 480 ommatidia per eye. In homozygous larvae, the mean cell size in third instar wing discs is reduced by 10-14%. Homozygotes, despite their smaller size, have almost twice as much lipids as wild-type males per milligram of fresh weight.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference
NOT Enhanced by
Statement
Reference
Suppressed by
Statement
Reference

chicoflp147E/chico1 has decreased cell number phenotype, suppressible by foxo21/foxo[+]

chicoflp147E/chico1 has visible phenotype, suppressible by foxo21/foxo[+]

NOT suppressed by
Statement
Reference

chicoflp147E/chico1 has decreased cell size phenotype, non-suppressible by foxo21/foxo[+]

Enhancer of
Statement
Reference
NOT Enhancer of
Statement
Reference

chicoflp147E is a non-enhancer of visible phenotype of upd1GMR.PB

NOT Suppressor of
Statement
Reference

chicoflp147E is a non-suppressor of visible phenotype of upd1GMR.PB

Other
Phenotype Manifest In
Enhanced by
Statement
Reference

chicoflp147E has eye phenotype, enhanceable by InR05545

chicoflp147E has wing phenotype, enhanceable by InR05545

chicoflp147E has adult phenotype, enhanceable by InR05545

Suppressed by
Statement
Reference

chicoflp147E/chico1 has wing phenotype, suppressible by foxo25/foxo21

chicoflp147E/chico1 has ommatidium phenotype, suppressible by foxo21/foxo[+]

chicoflp147E/chico1 has wing phenotype, suppressible by foxo21/foxo[+]

NOT suppressed by
Statement
Reference

chicoflp147E/chico1 has ommatidium phenotype, non-suppressible by foxo21/foxo[+]

Enhancer of
Statement
Reference
NOT Enhancer of
Statement
Reference

chicoflp147E is a non-enhancer of eye phenotype of upd1GMR.PB

NOT Suppressor of
Statement
Reference

chicoflp147E is a non-suppressor of eye phenotype of upd1GMR.PB

Additional Comments
Genetic Interactions
Statement
Reference

There is no further weight reduction in chicoflp147E/chico1, srlKG08646 double mutants.

Homozygous chico;foxo double mutants have more, and even slightly smaller, cells than homozygous chico single mutants.

Df(2L)flp170B/Df(2L)flp147E animals in which the eyes are composed of homozygous Df(2L)flp170B clones (generated using the "eyFLP" system) and in which the deletion of bsk in Df(2L)flp170B and Df(2L)flp147E is rescued by the presence of bsk+t5 have enlarged eyes, despite the reduced size of the structures around them. Other structures in the head and body that are unaffected by the eyFLP system appear to be maintained in proportion to the chico mutant body.

The phenotype of homozygous chicoflp147E flies is enhanced by a copy of InR05545. Overall wing size is reduced by 17.7%, the number of cells reduced by 19.8%. In the eye, the number of ommatidia and size of the eye is also reduced.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Rescued by
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
References (17)