FlyBase Allele Report: Dmel\chico[flp147E]

General Information

Symbol

Dmel\chico^flp147E

Species

D. melanogaster

Name

FlyBase ID

FBal0057437

Feature type

allele

Associated gene

Dmel\chico

Associated Insertion(s)

Carried in Construct

Also Known As

chico²

Key Links

Allele class

amorphic allele - molecular evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Naganos et al., 2012)

Mutagen

P-element activity

(Bohni et al., 1999, Riesgo-Escovar et al., 1996)

Progenitor genotype

P{ry11}chico¹

(Bohni et al., 1999)

chico¹

(Riesgo-Escovar et al., 1996)

Caused by aberration

Df(2L)flp147E

Cytology

Description

This is an imprecise excision of the P-element from chico¹. The translation start site and regulatory region are removed.

(Bohni et al., 1999)

Breaks in the chico transcription unit, removing the 5' portion of the transcription unit.

(Riesgo-Escovar et al., 1996)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of glucose metabolism disease

CEA with chico¹

(Naganos et al., 2012)

model of amnestic disorder

CEA with chico¹

(Naganos et al., 2012)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal learning (with chico¹)

(Naganos et al., 2012)

decreased body size

(Bohni et al., 1999)

decreased body size (with chico¹)

(Tiefenböck et al., 2010, Junger et al., 2003)

decreased body size | somatic clone

(Goberdhan and Wilson, 2002)

decreased cell number

(Bohni et al., 1999)

decreased cell number (with chico¹)

(Junger et al., 2003)

decreased cell size

(Bohni et al., 1999)

decreased cell size (with chico¹)

(Junger et al., 2003)

partially lethal - majority die (with chico¹)

(Naganos et al., 2012)

radiation sensitive | recessive

(Jaklevic et al., 2006)

viable

(Jaklevic et al., 2006, Bohni et al., 1999)

visible (with chico¹)

(Junger et al., 2003)

visible | recessive

(Bohni et al., 1999)

Phenotype Manifest In

adult (with chico¹)

(Junger et al., 2003)

embryonic/larval tracheal dorsal trunk

(Beitel and Krasnow, 2000)

embryonic/larval tracheal transverse connective

(Beitel and Krasnow, 2000)

eye

(Bohni et al., 1999)

eye (with chico¹)

(Junger et al., 2003)

eye | somatic clone

(Goberdhan and Wilson, 2002)

ommatidium (with chico¹)

(Junger et al., 2003)

ovary (with chico¹)

(Werz et al., 2009)

wing

(Bohni et al., 1999)

wing (with chico¹)

(Junger et al., 2003)

wing disc

(Bohni et al., 1999)

Detailed Description

Statement

Reference

Homozygous and chico¹/chico^flp147E adults show normal odour and electric shock reactivity.

Homozygous and chico¹/chico^flp147E adults show significant impairment in 3 minute, 1 hour and 3 hour memory after olfactory aversive conditioning.

(Naganos et al., 2012)

Compared to wild-type, chico^flp147E/chico¹ mutants are reduced in weight.

(Tiefenböck et al., 2010)

chico¹/chico^flp147E flies have elevated levels of lipids compared to control flies.

(Werz et al., 2009)

Homozygous pupae are more sensitive to X rays than wild type or heterozygous sibling controls.

(Jaklevic et al., 2006)

chico¹/chico^flp147E flies show lower body weight, fewer ommatidia, smaller wings, fewer cells per wing and smaller wing cell area than wild type.

(Junger et al., 2003)

Mosaic animals in which the eye is largely homozygous for chico^flp147E (generated using the "eyFLP" system) have small eyes.

(Goberdhan and Wilson, 2002)

The diameters of the dorsal trunk and transverse connective branches of the tracheal system are unchanged from wild type in Df(2L)flp147E larvae, while their lengths are reduced by 27% compared to wild type.

(Beitel and Krasnow, 2000)

Homozygous chico^flp147E mutants (which are homozygous for Df(2L)flp147E - this causes the alleles chico^flp147E and bsk^flp147E, and also carrying the bsk^+t5 rescue construct on the same chromosome to rescue the bsk^flp147E mutation) have a drastic weight reduction (65% in females, 55% in males) compared to wild-type. Body size reduction is observed at all developmental stages but does not alter the overall proportions of the flies. This is caused by a reduction in both cell size and number. In the wing, reduction in cell number accounts for 68% of the total reduction in wing size; the remaining 32% is due to the reduction in the average size of mutant cells. In homozygotes the ommatidial number is reduced by approximately 40%, from about 780 to about 480 ommatidia per eye. In homozygous larvae, the mean cell size in third instar wing discs is reduced by 10-14%. Homozygotes, despite their smaller size, have almost twice as much lipids as wild-type males per milligram of fresh weight.

(Bohni et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

chico^flp147E/chico¹ has decreased cell size phenotype, enhanceable by foxo²⁵/foxo²¹

(Junger et al., 2003)

NOT Enhanced by

Statement

Reference

chico^flp147E/chico¹ has decreased body size phenotype, non-enhanceable by srl^KG08646

(Tiefenböck et al., 2010)

Suppressed by

Statement

Reference

chico^flp147E/chico¹ has decreased body size phenotype, suppressible | partially by foxo²⁵/foxo²¹

(Junger et al., 2003)

chico^flp147E/chico¹ has decreased cell number phenotype, suppressible by foxo²¹/foxo[+]

(Junger et al., 2003)

chico^flp147E/chico¹ has decreased cell number phenotype, suppressible by foxo²⁵/foxo²¹

(Junger et al., 2003)

chico^flp147E/chico¹ has visible phenotype, suppressible by foxo²¹/foxo[+]

(Junger et al., 2003)

chico^flp147E/chico¹ has visible phenotype, suppressible by foxo²⁵/foxo²¹

(Junger et al., 2003)

NOT suppressed by

Statement

Reference

chico^flp147E/chico¹ has decreased cell size phenotype, non-suppressible by foxo²¹/foxo[+]

(Junger et al., 2003)

Enhancer of

Statement

Reference

chico^flp147E is an enhancer of visible phenotype of Pi3K92E^{D954A.UAS.Tag:MYC}, Scer\GAL4^Bx-MS1096

(Coelho et al., 2005)

NOT Enhancer of

Statement

Reference

chico^flp147E is a non-enhancer of visible phenotype of upd1^GMR.PB

(Bach et al., 2003)

NOT Suppressor of

Statement

Reference

chico^flp147E is a non-suppressor of visible phenotype of upd1^GMR.PB

(Bach et al., 2003)

chico^flp147E/chico^flp147E is a non-suppressor of increased body size | recessive | somatic clone phenotype of Pten¹

(Goberdhan and Wilson, 2002)

Other

Statement

Reference

Lnk^6S2/Lnk^4Q3, chico^flp147E/chico¹ has lethal phenotype

(Werz et al., 2009)

chico^flp147E, grp⁰⁶⁰³⁴ has lethal | recessive phenotype

(Jaklevic et al., 2006)

Phenotype Manifest In

Enhanced by

Statement

Reference

chico^flp147E/chico¹ has ommatidium phenotype, enhanceable by foxo²⁵/foxo²¹

(Junger et al., 2003)

chico^flp147E has eye phenotype, enhanceable by InR⁰⁵⁵⁴⁵

(Bohni et al., 1999)

chico^flp147E has wing phenotype, enhanceable by InR⁰⁵⁵⁴⁵

(Bohni et al., 1999)

chico^flp147E has adult phenotype, enhanceable by InR⁰⁵⁵⁴⁵

(Bohni et al., 1999)

Suppressed by

Statement

Reference

chico^flp147E/chico¹ has ommatidium phenotype, suppressible by foxo²¹/foxo[+]

(Junger et al., 2003)

chico^flp147E/chico¹ has ommatidium phenotype, suppressible by foxo²⁵/foxo²¹

(Junger et al., 2003)

chico^flp147E/chico¹ has wing phenotype, suppressible by foxo²¹/foxo[+]

(Junger et al., 2003)

chico^flp147E/chico¹ has wing phenotype, suppressible by foxo²⁵/foxo²¹

(Junger et al., 2003)

NOT suppressed by

Statement

Reference

chico^flp147E/chico¹ has ommatidium phenotype, non-suppressible by foxo²¹/foxo[+]

(Junger et al., 2003)

Enhancer of

Statement

Reference

chico^flp147E is an enhancer of wing phenotype of Pi3K92E^{D954A.UAS.Tag:MYC}, Scer\GAL4^Bx-MS1096

(Coelho et al., 2005)

NOT Enhancer of

Statement

Reference

chico^flp147E is a non-enhancer of eye phenotype of upd1^GMR.PB

(Bach et al., 2003)

NOT Suppressor of

Statement

Reference

chico^flp147E is a non-suppressor of eye phenotype of upd1^GMR.PB

(Bach et al., 2003)

chico^flp147E/chico^flp147E is a non-suppressor of eye | somatic clone phenotype of Pten¹

(Goberdhan and Wilson, 2002)

Additional Comments

Genetic Interactions

Statement

Reference

There is no further weight reduction in chico^flp147E/chico¹, srl^KG08646 double mutants.

(Tiefenböck et al., 2010)

Homozygous chico;foxo double mutants have more, and even slightly smaller, cells than homozygous chico single mutants.

(Junger et al., 2003)

Df(2L)flp170B/Df(2L)flp147E animals in which the eyes are composed of homozygous Df(2L)flp170B clones (generated using the "eyFLP" system) and in which the deletion of bsk in Df(2L)flp170B and Df(2L)flp147E is rescued by the presence of bsk^+t5 have enlarged eyes, despite the reduced size of the structures around them. Other structures in the head and body that are unaffected by the eyFLP system appear to be maintained in proportion to the chico mutant body.

(Goberdhan and Wilson, 2002)

The phenotype of homozygous chico^flp147E flies is enhanced by a copy of InR⁰⁵⁵⁴⁵. Overall wing size is reduced by 17.7%, the number of cells reduced by 19.8%. In the eye, the number of ommatidia and size of the eye is also reduced.

(Bohni et al., 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

chico^flp147E is rescued by chico^+t8

(Bohni et al., 1999)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

chico^1/2

(Tiefenböck et al., 2010)

chico²

(Sanaki et al., 2020, Radermacher et al., 2014, Naganos et al., 2012, Bartlett et al., 2011, Werz et al., 2009, Wang et al., 2008, Jaklevic et al., 2006, Coelho et al., 2005, Bach et al., 2003, Junger et al., 2003, Goberdhan and Wilson, 2002, Karr, 2001, Beitel and Krasnow, 2000, Bohni et al., 1999)

chico^flp147E

Name Synonyms

Secondary FlyBase IDs

References (17)

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