A small number of homozygotes reach the third larval instar stage. These larvae show an increase in the duration of this larval stage and develop melanotic masses.
Heterozygous InR05545 mutant flies reared on food containing 7.5% ethanol exhibit reduced adult viability compared to controls. Viability is comparable with controls when flies are raised on conventional media.
InR05545/InR3T5 mutant adult flies have a reduced body weight compared to heterozygous controls. Wing area is also reduced compared to controls.
Lipid content is increased in InR05545/InR3T5 mutant flies compared to controls. Carbohydrate content is similar to controls.
InR05545/InR3T5 mutant flies exhibit normal electroretinogram (ERG) readings.
The frequency of proliferating cells in the intestine of aging InRE19/InR05545 flies is significantly lower than that of age-matched controls.
The average number of germline stem cells (GSCs) in the testis of InRE19/InR05545 males within 24 hours of eclosion is similar to that seen in wild-type males at this time. However, the mutant males show an increased rate of reduction in GSC number with age compared to wild-type males, such that 14 days after eclosion, while the wild-type males maintain 90% of the GSC numbers of newly eclosed males, the mutant males maintain only 75% of the average GSC number of newly eclosed males.
InRE19/InR05545 males that are within 24 hours of eclosion have a reduced number of stage S2b spermatocyte cysts in the testis compared to wild-type males.
The average diameter of stage S6 InRE19/InR05545 spermatocytes is smaller than that of control spermatocytes at this stage.
The severity of cocaine-induced follicle degeneration in InR05545/+ females is indistinguishable from that observed in wild-type females.
Homozygous clones in the eye contain ommatidia which are smaller than normal.
Heterozygous males and females show a moderate reduction in life expectancy compared to controls (from 32.3 to 23.7 and from 35.5 to 30.5 days respectively) and are normal in size. InRE19/InR05545 adults are dwarf in size. Developmental rate is slower than normal. Females show an 85% increase in life expectancy compared to controls (from 32.3 to 60.1 days). Males show a high mortality as early adults, but have reduced mortality at late ages, resulting in a life expectancy at 10 days that is 43% greater than that of controls. InR05545/InRE19 females require continuous exposure to methoprene to induce any vitellogenesis. Treatment with methoprene reduces the longevity of InR05545/InRE19 females towards wild-type levels.