Q61term | Tsc1-PA
Site of nucleic acid difference inferred by FlyBase based on reported amino acid change
Tsc129 does not protect Df(1)su(s)R194/+ clones in the eye; Df(1)su(s)R194/+ ; Tsc129 clones are not recovered in the adult eye in animals with mosaic eyes containing two genotypes of cells with respect to RpL36; cells which are Df(1)su(s)R194/+ and cells in which the haplo-insufficiency of Df(1)su(s)R194/+ for RpL36 has been rescued by RpL36+t4 (in a wild-type background the Df(1)su(s)R194/+ clones are eliminated by cell competition and are not seen in the adult eye in these animals). Also, Tsc129 does not prevent apoptosis of Df(1)su(s)R194/+ cells in the wing.
Imaginal disc cells double mutant for Hsc70-4e3 and Tsc129 exhibit a synergistic increase in cell size as compared with their single mutant counterparts. There is an exacerbation of Tsc129-induced tissue overgrowth in the presence of the Hsc70-4e3 mutation, in some cases resulting in a marked outgrowth of tissue in the anterior portion of the retina.
The pathKG06640 growth and developmentally delayed phenotypes are significantly rescued by the presence of combined heterozygous Tsc129 and gig192 mutations. About 10% of rescued females are able to lay a small number of eggs, some of which can develop into adult flies.
The first instar larval lethality due to Tsc129 is partially suppressed by heterozygosity for Tor2L1 : 31% (n = 193) of Tor2L1/+ Tsc129/Tsc129 animals survive to the pupal stage. The increased cell size seen in somatic clones of Tsc129 in the eye is partially suppressed in a Tor2L1 heterozygous background: the resulting clone cells are 1.3X the size of those outside the clone, compared to 1.9X for Tsc129 somatic clone cells in a wild-type background. Cells in Tor2L1; Tsc129 double mutant somatic clones are approximately 0.25 times the size of their heterozygous neighbours - i.e.- have little or no size difference with Tor2L1 somatic clone cells.
Clones that are homozygous for both gig192 and Tsc129 show an identical phenotype to homozygous clones of either gig192 or Tsc129 alone. Ptendj189, Tsc129 double mutant somatic clones show an additive cell size increase phenotype. Tsc129 suppresses the lethality of homozygous InR35 or InR353/InR05545, allowing 6.6% and 39% animals to survive to adults respectively.