Akt11 mutant larval brains show abnormal accumulations of POSH protein.
Akt11/Akt13 mutant adult flies have a reduced body weight compared to heterozygous controls. Wing area is also reduced compared to controls.
Lipid content is increased in Akt11/Akt13 mutant flies compared to controls. Carbohydrate content is similar to controls.
Akt11/Akt13 mutant flies exhibit defective electroretinogram (ERG) readings.
Cholinesterase activity is significantly reduced in Akt11/Akt13 mutant flies compared to controls.
8% of Akt13/Akt11 mutant wings show small ectopic vein-like patches. No ectopic wing vein is seen in either heterozygote.
Eye size is reduced in an Akt11 heterozygous background.
Homozygous clones in the eye show severe undergrowth and the eye is severely reduced compared to wild type.
Long term synaptic depression at larval neuromuscular junctions is strongly impaired in Akt104226/Akt11 third instar larvae. This effect is seen when LTD is tested using 0.2 or 0.4 mM Ca2+ saline or when high frequency stimulation (40Hz) is used.
No effect is seen on long term synaptic depression at larval neuromuscular junctions in Akt11/+ animals.
When Akt11/Akt11; Akt1hs.PS animals are raised at 25'C with daily heat-shocks and then switched to 18'C for 24-36 hours following the last heat shock and prior to harvesting as late third instar larvae, long term synaptic depression (LTD) at neuro-muscular junctions is severely disrupted. However LTD is normal if the heat shock regime is continued to the time of testing.
Head capsule reduced - pinhead phenotype.
Ommatidial size in Akt11/Akt13 flies is reduced to 0.65 of wild-type.
Homozygous mutant clones in the eye have smaller cells and are rarely recovered in adults.
Akt11 germ-line clones lead to 100% maternal effect embryonic lethality with embryonic extensive cell death. Akt11 homozygotes die as larvae, and appear to be phenotypically identical to Akt11/Df(3R)sbd45 animals. Stage 16 Akt11/Df(3R)sbd45 embryos have only very mild tracheal branching defects.
Homozygous clones in the eye contain ommatidia which are smaller than normal.
Akt11 mutants lacking both maternal and zygotic Akt1 function exhibit ectopic and widespread apoptosis.
Homozygous Akt11 mutant larvae, pupae and adults are significantly smaller in size than Akt11/+ heterozygous animals.
Cells of somatic clones of Akt11 mutant cells in the wing are smaller in size than their Akt11/+ neighbours. Flow cytometry of dissociated Akt11 mutant wing imaginal cells confirms that the mutant cells are smaller than similarly treated wild-type cells.
Eyes composed of somatic clones of Akt11 mutant cells are smaller in size as compared to wild-type eyes.
Rhabdomeres are reduced in size in homozygous clones in the eye. In mosaic ommatidia, both small and normal sized rhabdomeres can be present. Homozygous clones are rare and small and are only obtained when they are induced during the third larval instar stage.
Embryos derived from germline clones lack portions of cuticle at the end of embryogenesis. In the absence of zygotic Akt1 embryos show almost complete loss of cuticle, embryos with some zygotic activity exhibit loss of cuticular head and dorsal structures. Heat induced expression of Akt1, maternally and zygotically, allows the reappearance of most cuticle structures. AO staining of embryos derived from germline clones shows extensive apoptosis. By stage 8 TUNEL signal reveals extensive DNA fragmentation.
Semi-viable. Homozygotes are fertile and hemizygotes are lethal.