FlyBase Allele Report: Dmel\Akt[3]

General Information

Symbol

Dmel\Akt³

Species

D. melanogaster

Name

FlyBase ID

FBal0124421

Feature type

allele

Associated gene

Dmel\Akt

Associated Insertion(s)

Carried in Construct

Also Known As

Akt1³, dPKB³, PKB³, dAkt³

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

Mutagen

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Stocker et al., 2002)

Mutagen

Progenitor genotype

Cytology

Description

Amino acid replacement: G99S.

(Stocker et al., 2002)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:16,101,481..16,101,481 [+]

Nucleotide change:

G16101481A

Amino acid change:

Reported amino acid change:

G99S

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Stocker et al., 2002)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of type 2 diabetes mellitus

CEA with Akt¹

(Murillo-Maldonado et al., 2011)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates frontotemporal dementia

modeled by Hsap\CHMP2B^Intron5.UAS

(West et al., 2018)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

ommatidium (with Akt¹)

(Stocker et al., 2003)

wing (with Akt¹)

(Murillo-Maldonado et al., 2011)

wing vein | ectopic (with Akt¹)

(Murillo-Maldonado et al., 2011)

Detailed Description

Statement

Reference

Homozygous Akt1³ mutants reared on 100g/l yeast food weigh approximately the same as wild-type flies reared on 10g/l yeast food. When reared on 10g/l yeast food, these mutants exhibit a delay in development but only mildly decreased size and weight compared to controls.

(Nowak et al., 2013)

Akt1¹/Akt1³ mutant adult flies have a reduced body weight compared to heterozygous controls. Wing area is also reduced compared to controls.

Lipid content is increased in Akt1¹/Akt1³ mutant flies compared to controls. Carbohydrate content is similar to controls.

Akt1¹/Akt1³ mutant flies exhibit defective electroretinogram (ERG) readings.

Cholinesterase activity is significantly reduced in Akt1¹/Akt1³ mutant flies compared to controls.

(Murillo-Maldonado et al., 2011)

8% of Akt1³/Akt1¹ mutant wings show small ectopic vein-like patches. No ectopic wing vein is seen in either heterozygote.

(Murillo-Maldonado et al., 2011)

Homozygotes have reduced body weight compared to controls.

(Reiling and Hafen, 2004)

Ommatidial size in Akt1¹/Akt1³ flies is reduced to 0.65 of wild-type.

(Stocker et al., 2003)

Homozygous adults are dwarf in size and are short-lived.

(Clancy et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Akt³ is an enhancer of visible | adult stage phenotype of Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PU

(West et al., 2018)

Suppressor of

Statement

Reference

Akt1[+]/Akt³ is a suppressor | partially of flightless phenotype of Pten^dj189/Pten⁵

(Mensah et al., 2015)

Akt³ is a suppressor of increased cell growth rate | nutrition conditional phenotype of Pten¹¹⁷

(Nowak et al., 2013)

Akt³ is a suppressor of increased cell growth rate phenotype of Pten¹¹⁷

(Nowak et al., 2013)

Akt¹/Akt³ is a suppressor of visible phenotype of Pi3K92E^UAS.Tag:MYC, Scer\GAL4^GMR.PF

(Radimerski et al., 2002)

Akt⁰⁴²²⁶/Akt³ is a suppressor of lethal | recessive | larval stage phenotype of Pten^unspecified

(Stocker et al., 2002)

Akt¹/Akt³ is a suppressor of lethal | recessive | larval stage phenotype of Pten^unspecified

(Stocker et al., 2002)

Akt³/Akt³ is a suppressor of lethal | recessive | larval stage phenotype of Pten^unspecified

(Stocker et al., 2002)

Other

Statement

Reference

Akt¹/Akt³, Hsap\FOXO3^UAS.cFa, Scer\GAL4^GMR.PF has visible phenotype

(Junger et al., 2003)

Akt¹/Akt³, Pten¹¹⁷ has viable phenotype

(Lee et al., 2003)

Akt³, Pten^unspecified has increased body size phenotype

(Stocker et al., 2002)

Akt⁰⁴²²⁶/Akt³, Pten^unspecified has increased body size phenotype

(Stocker et al., 2002)

Phenotype Manifest In

Enhancer of

Statement

Reference

Akt³ is an enhancer of eye phenotype of Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PU

(West et al., 2018)

Suppressor of

Statement

Reference

Akt³ is a suppressor of eye phenotype of Pten¹¹⁷

(Nowak et al., 2013)

Akt³ is a suppressor of eye | nutrition conditional phenotype of Pten¹¹⁷

(Nowak et al., 2013)

Akt¹/Akt³ is a suppressor of eye phenotype of Pten^dj189/Pten¹¹⁷

(Pinal et al., 2006)

Akt¹/Akt³ is a suppressor of rhabdomere phenotype of Pten^dj189/Pten¹¹⁷

(Pinal et al., 2006)

Akt¹/Akt³ is a suppressor of eye phenotype of Pi3K92E^UAS.Tag:MYC, Scer\GAL4^GMR.PF

(Radimerski et al., 2002)

Akt¹/Akt³ is a suppressor of ommatidium phenotype of Pi3K92E^UAS.Tag:MYC, Scer\GAL4^GMR.PF

(Radimerski et al., 2002)

NOT Suppressor of

Statement

Reference

Akt¹/Akt³ is a non-suppressor of ommatidium phenotype of Rheb^EP50.084, Scer\GAL4^GMR.PF

(Stocker et al., 2003)

Other

Statement

Reference

Akt¹/Akt³, Pten^dj189/Pten¹¹⁷ has lipid particle & nurse cell phenotype

(Vereshchagina and Wilson, 2006)

Akt¹/Akt³, Hsap\FOXO3^UAS.cFa, Scer\GAL4^GMR.PF has eye phenotype

(Junger et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

An Akt1³ mutant background completely suppresses the Pten¹¹⁷ overgrowth phenotype, under normal as well as under starvation conditions.

(Nowak et al., 2013)

The rhabdomere defects seen in Pten¹¹⁷/Pten^dj189 mutant fly eyes are rescued in a Akt1¹/Akt1³ mutant background. In these animals photoreceptor cell morphology is close to wild-type and eyes display a low frequency of deformed rhabdomeres.

(Pinal et al., 2006)

The nurse cells of Pten^dj189/Pten¹¹⁷; Akt1³/Akt1¹ viable females show normal lipid storage, while clones of Pten alleles show formation of large lipid droplets.

(Vereshchagina and Wilson, 2006)

scyl³¹/scyl³¹ partially suppresses the reduced body weight phenotype of Akt1³ homozygous flies.

(Reiling and Hafen, 2004)

The large size and disorganisation of ommatidia in Scer\GAL4^GMR.PF/+; Rheb^EP50.084/+ flies is unaffected by Akt1¹/Akt1³.

(Stocker et al., 2003)

The enlarged eye and ommatidia phenotype caused by expression of Pi3K92E^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^GMR.PF is completely suppressed in a Akt1¹/Akt1³ background.

(Radimerski et al., 2002)

Pten^unspecified flies rescued by the combination Akt1¹/Akt1³ are wild-type in size. Pten^unspecified ; Akt1¹/Akt1³ flies have essentially normal ommatidia and rhabdomeres in the eye and the wings show normal venation.

(Stocker et al., 2002)

Xenogenetic Interactions

Statement

Reference

The eye degeneration phenotype characteristic for adult flies expressing Hsap\CHMP2B^Intron5.UAS under the control of Scer\GAL4^GMR.PU is strongly enhanced by combination with Akt1³.

(West et al., 2018)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

AKT³

(West et al., 2018)

Akt1³

(Amoyel et al., 2016, Mensah et al., 2015, Murillo-Maldonado et al., 2011, Pinal et al., 2006)

Akt³

(Zheng and Sehgal, 2010, Vereshchagina and Wilson, 2006)

PKB³

(Nowak et al., 2013, Murillo-Maldonado et al., 2011, Stocker et al., 2003, Clancy et al., 2001)

akt³

(Gao et al., 2024, Borreguero-Muñoz et al., 2019, Bardet et al., 2013, Reiling and Hafen, 2004)

dAkt³

(Lee et al., 2003, Stocker et al., 2002)

dPKB³

(Wittwer et al., 2005, Junger et al., 2003, Oldham and Hafen, 2003, Radimerski et al., 2002)

Name Synonyms

Secondary FlyBase IDs

References (21)

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