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General Information
Symbol
Dmel\Akt3
Species
D. melanogaster
Name
FlyBase ID
FBal0124421
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
Akt13, dPKB3, PKB3, dAkt3
Key Links
Mutagen
    Nature of the Allele
    Mutagen
    Mutations Mapped to the Genome
     
    Type
    Location
    Additional Notes
    References
    Nucleotide change:

    G16101481A

    Amino acid change:

    G99S | Akt1-PA; G99S | Akt1-PB; M1T | Akt1-PC; G99S | Akt1-PD; G99S | Akt1-PE

    Reported amino acid change:

    G99S

    Comment:

    Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

    Associated Sequence Data
    DNA sequence
    Protein sequence
     
     
    Progenitor genotype
    Cytology
    Nature of the lesion
    Statement
    Reference

    Amino acid replacement: G99S.

    Expression Data
    Reporter Expression
    Additional Information
    Statement
    Reference
     
    Marker for
    Reflects expression of
    Reporter construct used in assay
    Human Disease Associations
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 1 )
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 1 )
    Disease
    Interaction
    References
    Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
     
    Disease-implicated variant(s)
     
    Phenotypic Data
    Phenotypic Class
    Phenotype Manifest In
    Detailed Description
    Statement
    Reference

    Homozygous Akt13 mutants reared on 100g/l yeast food weigh approximately the same as wild-type flies reared on 10g/l yeast food. When reared on 10g/l yeast food, these mutants exhibit a delay in development but only mildly decreased size and weight compared to controls.

    Akt11/Akt13 mutant adult flies have a reduced body weight compared to heterozygous controls. Wing area is also reduced compared to controls.

    Lipid content is increased in Akt11/Akt13 mutant flies compared to controls. Carbohydrate content is similar to controls.

    Akt11/Akt13 mutant flies exhibit defective electroretinogram (ERG) readings.

    Cholinesterase activity is significantly reduced in Akt11/Akt13 mutant flies compared to controls.

    8% of Akt13/Akt11 mutant wings show small ectopic vein-like patches. No ectopic wing vein is seen in either heterozygote.

    Homozygotes have reduced body weight compared to controls.

    Ommatidial size in Akt11/Akt13 flies is reduced to 0.65 of wild-type.

    Homozygous adults are dwarf in size and are short-lived.

    External Data
    Interactions
    Show genetic interaction network for Enhancers & Suppressors
    Phenotypic Class
    Enhancer of
    Statement
    Reference
    Suppressor of
    Statement
    Reference

    Akt1[+]/Akt3 is a suppressor | partially of flightless phenotype of Ptendj189/Pten5

    Akt3 is a suppressor of increased cell growth | nutrition conditional phenotype of Pten117

    Akt3 is a suppressor of increased cell growth phenotype of Pten117

    Other
    Phenotype Manifest In
    Enhancer of
    Statement
    Reference
    Suppressor of
    Statement
    Reference

    Akt3 is a suppressor of eye | nutrition conditional phenotype of Pten117

    Akt3 is a suppressor of eye phenotype of Pten117

    Akt1/Akt3 is a suppressor of eye phenotype of Ptendj189/Pten117

    Akt1/Akt3 is a suppressor of rhabdomere phenotype of Ptendj189/Pten117

    NOT Suppressor of
    Statement
    Reference
    Other
    Statement
    Reference
    Additional Comments
    Genetic Interactions
    Statement
    Reference

    An Akt13 mutant background completely suppresses the Pten117 overgrowth phenotype, under normal as well as under starvation conditions.

    The rhabdomere defects seen in Pten117/Ptendj189 mutant fly eyes are rescued in a Akt11/Akt13 mutant background. In these animals photoreceptor cell morphology is close to wild-type and eyes display a low frequency of deformed rhabdomeres.

    The nurse cells of Ptendj189/Pten117; Akt13/Akt11 viable females show normal lipid storage, while clones of Pten alleles show formation of large lipid droplets.

    scyl31/scyl31 partially suppresses the reduced body weight phenotype of Akt13 homozygous flies.

    The large size and disorganisation of ommatidia in Scer\GAL4GMR.PF/+; RhebEP50.084/+ flies is unaffected by Akt11/Akt13.

    The enlarged eye and ommatidia phenotype caused by expression of Pi3K92EScer\UAS.T:Hsap\MYC under the control of Scer\GAL4GMR.PF is completely suppressed in a Akt11/Akt13 background.

    Ptenunspecified flies rescued by the combination Akt11/Akt13 are wild-type in size. Ptenunspecified ; Akt11/Akt13 flies have essentially normal ommatidia and rhabdomeres in the eye and the wings show normal venation.

    Xenogenetic Interactions
    Statement
    Reference

    The eye degeneration phenotype characteristic for adult flies expressing Hsap\CHMP2BIntron5.UAS under the control of Scer\GAL4GMR.PU is strongly enhanced by combination with Akt13.

    Complementation and Rescue Data
    Comments
    Images (0)
    Mutant
    Wild-type
    Stocks (0)
    Notes on Origin
    Discoverer
    External Crossreferences and Linkouts ( 0 )
    Synonyms and Secondary IDs (7)
    References (20)