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General Information
Symbol
Dmel\Akt04226
Species
D. melanogaster
Name
FlyBase ID
FBal0243540
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
Akt104226, l(3)04226
Key Links
Nature of the Allele
Expression Data
Reporter Expression
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Information
Statement
Reference

A lacZ reporter in this P-element insertion allowed the zygotic pattern of expression of Akt1 to be deduced. The reporter is expressed in the tracheal placodes in a pattern similar to that of trh and btl.

 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Emergence of homozygous Akt104226 mutant flies is delayed by two to four days compared with controls.

The eyes of homozygous Akt104226 mutant flies exhibit a decrease in both ommatidial number and size compared with controls. The ommatidia in Akt104226 mutant eye clones are reduced in size compared to the homozygous flies.

Akt104226 heterozygous mutants exhibit extreme sensitivity to 1-octen-3-ol. These flies survive for approximately 13 days when exposed to 0.5ppm 1-octen-3-ol, significantly lower than the 18 days-long survival span observed for exposed wild-type flies.

The neuromuscular junctions of homozygous Akt104226 mutant larvae (muscle 4 of segment A2-4) show an ~80% increase in synaptic growth compared to controls. These mutants also have an increase in brp positive active zones and a decrease in brp intensity.

The neuromuscular junctions of Akt104226/Df(3R)Exel7328 mutant larvae (muscle 4 of segment A2-4) show a significant increase in synaptic growth compared to controls. These mutants also have an increase in brp positive active zones.

No retinal phenotypes are seen in Akt104226 mutant flies.

Akt104226/Akt11 flies are about half the weight of control animals.

Long term synaptic depression at larval neuromuscular junctions is strongly impaired in Akt104226/Akt11 third instar larvae. This effect is seen when LTD is tested using 0.2 or 0.4 mM Ca2+ saline or when high frequency stimulation (40Hz) is used. The same phenotype is seen in Akt104226 homozygotes.

Reduction of Akt1 activity decreases synapse number in both Akt104226 heterozygous and homozygous animals.

Germline clones produce eggs with poor cuticle development.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement
Reference
Enhancer of
NOT Enhancer of
Statement
Reference
Suppressor of
NOT Suppressor of
Statement
Reference
Other
Phenotype Manifest In
Enhanced by
Statement
Reference

Akt104226/Akt04226 has eye phenotype, enhanceable by foxo25

Suppressed by
Statement
Reference
Enhancer of
Statement
Reference

Akt04226 is an enhancer of eye phenotype of foxo25

Akt04226 is an enhancer of ommatidium phenotype of foxo25

NOT Enhancer of
Statement
Reference
Suppressor of
NOT Suppressor of
Statement
Reference

Akt04226 is a non-suppressor of eye phenotype of foxo25

Akt04226 is a non-suppressor of ommatidium phenotype of foxo25

Akt1[+]/Akt04226 is a non-suppressor of eye | adult stage phenotype of MagiUAS.GFP, Scer\GAL4GMR.PU

Akt1[+]/Akt04226 is a non-suppressor of embryonic/larval plasmatocyte | ectopic phenotype of egh62d18

Akt1[+]/Akt04226 is a non-suppressor of eye disc | somatic clone | cell non-autonomous phenotype of Uba1A1

Other
Additional Comments
Genetic Interactions
Statement
Reference

The reduction in ommatidium area seen in either mutant alone is enhanced in Akt104226 foxo25 double mutant flies. As in the foxo25 mutant alone, ommatidium number is increased.

The rough eye phenotype observed in adults expressing MagiScer\UAS.T:Avic\GFP under the control of Scer\GAL4GMR.PU is not modified by combination with single copy of Akt104226.

A Akt104226 heterozygous background results in a reduction in the size of fat cells by around 50% in flies expressing CdsANIG.7962R under the control of Scer\GAL4ppl.PP.

The neuromuscular junctions of Akt104226/+ gig109/+ mutant larvae (muscle 4 of segment A2-4) exhibit increased synaptic growth in comparison with the single heterozygote controls.

The rough eye phenotype caused by expression of polyScer\UAS.cBa under the control of Scer\GAL4GMR.PU is suppressed by Akt104226/+.

Heterozygous Akt104226 strongly suppresses the egh62d18 larval nerve phenotype regarding nerve diameter, but not the nerve-attached plasmatocyte-phenotype.

Akt104226/+ strongly enhances the GirdindsRNA.Scer\UAS, Scer\GAL4en-e16E wing phenotype, with a dramatic reduction in cell size and posterior wing compartment area.

A Akt104226/+ background has no effect on the non-autonomous overgrowth phenotype found in Uba1A1 mosaic eye clones.

Akt104226/Akt11 flies heterozygous for wdbIP show a significant increase in body size and weight.

Xenogenetic Interactions
Statement
Reference

The eye degeneration phenotype characteristic for adult flies expressing Hsap\CHMP2BIntron5.UAS under the control of Scer\GAL4GMR.PU is strongly enhanced by combination with Akt104226.

Heterozygosity for Akt104226 does not modify the fully penetrant rough eye phenotype resulting from the co-expression of HPV18\E6Scer\UAS.T:Hsap\MYC and Hsap\UBE3AScer\UAS.cRa under the control of Scer\GAL4GMR.PU, leading to severe eye necrosis.

One copy of Akt104226 enhances the retinal phenotype seen when Hsap\HTT128Q.1-336.Scer\UAS is expressed under the control of Scer\GAL4GMR.PU.

One copy of Akt104226 suppresses the external eye and retinal phenotypes seen when Hsap\ATXN182Q.Scer\UAS is expressed under the control of Scer\GAL4GMR.PU.

The eye phenotype caused by expression of Mmus\Mdm2Scer\UAS.cFa under the control of Scer\GAL4GMR.PF is not rescued if the flies are also carrying either Akt104226.

Complementation and Rescue Data
Fails to complement
Partially rescued by
Comments

Expression of Akt1Scer\UAS.cSa under the control of Scer\GAL4arm.PU partially rescues the reductions in ommatidial size and number seen in homozygous Akt104226 mutant flies.

Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer

A. Spradling.

Comments
Comments

Complements: l(3)0520300934. Complements: srp01549. Complements: l(3)0520308235. Complements: l(3)j1A1j1A1. Complements: srpneo45. Complements: l(3)neo461. Complements: l(3)neo471.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (14)
References (37)