Emergence of homozygous Akt104226 mutant flies is delayed by two to four days compared with controls.
The eyes of homozygous Akt104226 mutant flies exhibit a decrease in both ommatidial number and size compared with controls. The ommatidia in Akt104226 mutant eye clones are reduced in size compared to the homozygous flies.
Akt104226 heterozygous mutants exhibit extreme sensitivity to 1-octen-3-ol. These flies survive for approximately 13 days when exposed to 0.5ppm 1-octen-3-ol, significantly lower than the 18 days-long survival span observed for exposed wild-type flies.
The neuromuscular junctions of homozygous Akt104226 mutant larvae (muscle 4 of segment A2-4) show an ~80% increase in synaptic growth compared to controls. These mutants also have an increase in brp positive active zones and a decrease in brp intensity.
The neuromuscular junctions of Akt104226/Df(3R)Exel7328 mutant larvae (muscle 4 of segment A2-4) show a significant increase in synaptic growth compared to controls. These mutants also have an increase in brp positive active zones.
No retinal phenotypes are seen in Akt104226 mutant flies.
Long term synaptic depression at larval neuromuscular junctions is strongly impaired in Akt104226/Akt11 third instar larvae. This effect is seen when LTD is tested using 0.2 or 0.4 mM Ca2+ saline or when high frequency stimulation (40Hz) is used. The same phenotype is seen in Akt104226 homozygotes.
Reduction of Akt1 activity decreases synapse number in both Akt104226 heterozygous and homozygous animals.
Germline clones produce eggs with poor cuticle development.