FlyBase Allele Report: Dmel\Cds[NIG.7962R]

General Information

Symbol

Dmel\Cds^NIG.7962R

Species

D. melanogaster

Name

FlyBase ID

FBal0260643

Feature type

allele

Associated gene

Dmel\Cds

Associated Insertion(s)

Carried in Construct

P{NIG.7962R}

Also Known As

UAS-CdsA RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Tian et al., 2011)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Tian et al., 2011)

Mutagen

in vitro construct

(Tian et al., 2011)

Progenitor genotype

Carried in construct

P{NIG.7962R}

Cytology

Description

UASt drives expression of an RNAi construct targeting CdsA.

(Tian et al., 2011)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-7962R

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates congenital generalized lipodystrophy

modeled by Seipin^null

(Tian et al., 2011)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased cell size, with Scer\GAL4^αTub84B.PL

(Liu et al., 2014)

Phenotype Manifest In

brain, with Scer\GAL4^αTub84B.PL

(Liu et al., 2014)

embryonic/larval fat body, with Scer\GAL4^ppl.PP

(Liu et al., 2014)

embryonic/larval fat body, with Scer\GAL4^αTub84B.PL

(Liu et al., 2014)

imaginal disc, with Scer\GAL4^αTub84B.PL

(Liu et al., 2014)

lipid droplet | ectopic, with Scer\GAL4^elav.PU

(Tian et al., 2011)

lipid droplet | ectopic, with Scer\GAL4^ppl.PP

(Tian et al., 2011)

salivary gland, with Scer\GAL4^elav.PU

(Tian et al., 2011)

salivary gland, with Scer\GAL4^ppl.PP

(Tian et al., 2011)

Detailed Description

Statement

Reference

Expression of CdsA^NIG.7962R in both the larval fat body and salivary gland, under the control of Scer\GAL4^ppl.PP, causes an ectopic lipid droplet phenotype.

Expression of CdsA^NIG.7962R under the control of Scer\GAL4^αTub84B.PL, results in these larvae accumulating excess fat in many non-adipose tissues, including the salivary gland, proventriculus, hind gut, Malpighian tubules, and trachea. Compared to controls, Scer\GAL4^αTub84B.PL-->CdsA^NIG.7962R animals exhibit a much higher level of triacylglycerol.

Expression of CdsA^NIG.7962R under the control of Scer\GAL4^αTub84B.PL results in a significant reduction in organ size, compared to controls. The imaginal discs, salivary glands, and brain are smaller in these flies, compared to controls. While cell number is not changed in these mutants, cell size is greatly reduced.

The levels of diacylglycerol and phosphatidylethanolamine are significantly increased in fat body cells expressing CdsA^NIG.7962R under the control of Scer\GAL4^αTub84B.PL.

(Liu et al., 2014)

Expression of CdsA^NIG.7962R using Scer\GAL4^ppl.PP or Scer\GAL4^elav.PU, but not Scer\GAL4^Sgs3.PD, results in ectopic lipid storage in the salivary gland.

(Tian et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Cds^NIG.7962R, Scer\GAL4^αTub84B.PL has decreased cell size phenotype, enhanceable by bbc^GD1980, Scer\GAL4^αTub84B.PL

(Liu et al., 2014)

Suppressed by

Statement

Reference

Cds^NIG.7962R, Scer\GAL4^ppl.PP has decreased cell size phenotype, suppressible by Akt^UAS.Exel, Scer\GAL4^ppl.PP

(Liu et al., 2014)

Cds^NIG.7962R, Scer\GAL4^ppl.PP has decreased cell size phenotype, suppressible by Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, Scer\GAL4^ppl.PP

(Liu et al., 2014)

Cds^NIG.7962R, Scer\GAL4^αTub84B.PL has decreased cell size phenotype, suppressible by Df(3R)Pi3K92E^A

(Liu et al., 2014)

Cds^NIG.7962R, Scer\GAL4^αTub84B.PL has decreased cell size phenotype, suppressible by Akt⁰⁴²²⁶

(Liu et al., 2014)

Phenotype Manifest In

Enhanced by

Statement

Reference

Cds^NIG.7962R, Scer\GAL4^ppl.PP has embryonic/larval fat body phenotype, enhanceable by bbc^GD1980, Scer\GAL4^ppl.PP

(Liu et al., 2014)

Suppressed by

Statement

Reference

Cds^NIG.7962R, Scer\GAL4^αTub84B.PL has embryonic/larval fat body phenotype, suppressible by Akt⁰⁴²²⁶

(Liu et al., 2014)

Cds^NIG.7962R, Scer\GAL4^αTub84B.PL has embryonic/larval fat body phenotype, suppressible by Df(3R)Pi3K92E^A

(Liu et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Overexpression of Akt1^{Scer\UAS.Exel} or constitutively active Pi3K92E^{Scer\UAS.T:Hsap\MYC,T:Hsap\CAAX} in the salivary glands, under the control of Scer\GAL4^ppl.PP, fully suppresses the small cell size phenotype found upon knockdown of CdsA through expression of CdsA^NIG.7962R. The ectopic lipid accumulation phenotype of CdsA^NIG.7962R mutants is partially rescued by overexpression of Pi3K92E^{Scer\UAS.T:Hsap\MYC,T:Hsap\CAAX} but not Akt1^{Scer\UAS.Exel}.

A Df(3R)Pi3K92E^A or Akt1⁰⁴²²⁶ heterozygous background results in a reduction in the size of fat cells by around 50% in flies expressing CdsA^NIG.7962R under the control of Scer\GAL4^ppl.PP.

CdsA^NIG.7962R and bbc^GD1980 double mutants exhibit a synergistic effect, resulting in a drastic decrease in fat cell size. Salivary gland cell size is unaffected.

(Liu et al., 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

NIG-Fly

7962R-1

P{NIG.7962R}1

7962R-2

P{NIG.7962R}2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Cds^NIG.7962R

CdsA^NIG.7962R

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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