FlyBase Allele Report: Dmel\Seipin[null]

General Information

Symbol

Dmel\Seipin^null

Species

D. melanogaster

Name

FlyBase ID

FBal0260636

Feature type

allele

Associated gene

Dmel\Seipin

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

amorphic allele - molecular evidence

Mutagen

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Tian et al., 2011)

Mutagen

ends-out gene targeting

(Tian et al., 2011)

FLPase

(Tian et al., 2011)

I-SceI

(Tian et al., 2011)

Progenitor genotype

Cytology

Description

An ends-out gene targeting event in which the whole genomic region of Seipin is replaced with Avic\GFP and w^+mC.

(Tian et al., 2011)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of congenital generalized lipodystrophy

CEA

(Bi et al., 2014, Tian et al., 2011)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of congenital generalized lipodystrophy

is ameliorated by mdy^QX25

(Tian et al., 2011)

is ameliorated by Pcyt1¹⁶⁹¹⁹

(Tian et al., 2011)

is ameliorated by Cds^EY08412

(Tian et al., 2011)

is exacerbated by mdy^QX25

(Tian et al., 2011)

is exacerbated by Cds¹

(Tian et al., 2011)

is exacerbated by mdy^EY07280

(Tian et al., 2011)

is ameliorated by SCAP^EY06708

(Tian et al., 2011)

is ameliorated by Lpin^GD14004

(Tian et al., 2011)

is exacerbated by mino^EY00734

(Tian et al., 2011)

is exacerbated by Cds^NIG.7962R

(Tian et al., 2011)

is ameliorated by mdy^EY07280

(Tian et al., 2011)

Comments on Models/Modifiers Based on Experimental Evidence ( 3 )

Lipodystrophy is characterised by reduced lipid storage in adipose tissue and ectopic lipid storage in non-adipose tissue. Seipin^null larvae show reduced lipid storage in fat bodies and ectopic lipid droplets in the salivary glands.

(Tian et al., 2011)

mdy^QX25 Seipin^null double mutant larvae show greatly reduced fat body lipid storage compared to either single mutant (an exacerbation of the lipodystrophy model in adipose tissue). However, the ectopic lipid storage phenotype seen in non-adipose tissue in Seipin^null single mutants is fully suppressed by mdy^QX25 (a suppression of the lipodystrophy model in non-adipose tissue).

(Tian et al., 2011)

Overexpression of mdy (using mdy^EY07280) restores fat body lipid storage levels to near wild-type in Seipin^null larvae (a suppression of the lipodystrophy model in adipose tissue). However, overexpression of mdy (using mdy^EY07280) increases the ectopic lipid storage phenotype observed in non-adipose tissue in Seipin^null larvae (an exacerbation of the lipodystrophy model in non-adipose tissue).

(Tian et al., 2011)

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased cell size | somatic clone

(Bi et al., 2014)

fertile

(Tian et al., 2011)

lethal | nutrition conditional

(Tian et al., 2011)

viable

(Tian et al., 2011)

Phenotype Manifest In

Detailed Description

Statement

Reference

Seipin^null mutant fat body cell clones have fewer and smaller lipid droplets than adjacent control cells. In addition, the mutant cells are smaller than the surrounding wild type cells. The salivary glands in Seipin^null mutants are similar in size to wild type.

The overall level of free fatty acids, especially C12:0 , C14:0 , C16:0 , C16:1 and C18:1 ) is greatly reduced in Seipin^null mutant third instar larvae.

Compared to wild-type, the calcium-dependent ATPase activity is significantly decreased in Seipin^null mutants.

After treatment with Thapsigargin (a Ca-P60A inhibitor) or Ionomycim (a calcium ionophore) in calcium-free solution, the rise in cytosolic calcium levels seen in Seipin^null mutant fat body cells is significantly lower than in wild type.

The basal ER calcium level is decreased in Seipin^null mutant fat cells compared to wild type. Cytosolic calcium concentration is increased.

(Bi et al., 2014)

Mutants have no noticeable behaviour defects.

Fat bodies of Seipin^null larvae and fat cells from young Seipin^null adults have significantly reduced lipid storage compared to wild type. This small lipid droplet phenotype is more sever under starvation conditions. The overall morphology of the fat body in Seipin^null mutants appears normal.

Total glyceride content in Seipin^null mutants is greatly reduced compared to controls.

The average weight of Seipin^null flies is lower than controls.

Under fed conditions, the amount of neutral lipids in oenocytes of Seipin^null animals is the same as wild type. Neutral lipids are higher in starved Seipin^null animals, but sill much lower than wild type.

Circulating glyceride levels in the hemolymph of Seipin^null mutants are much lower than in wild type.

Under starved conditions, 100% of Seipin^null flies die within 42 hours, whereas more than half of control flies survive for 48 hours.

The proventriculus and anterior midgut region of Seipin^null mutants contain abnormally large lipid droplets and more stored lipid, respectively, compared to controls.

Seipin^null mutants exhibit ectopic lipid droplets in the salivary gland, which normally lacks any visible lipid droplets.

Seipin^null mutant larvae have increased levels of total and most individual species of phosphatidic acid.

The salivary glands of Seipin^null mutant larvae have increased levels of total and several species of diacylglycerol.

In Seipin^null mutants, the level of triacylglycerol (TAG) is greatly reduced in the fat body, while the levels of several TAG species are significantly increased in salivary glands.

(Tian et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

Seipin^null has salivary gland phenotype, enhanceable by mino^EY00734/Scer\GAL4^48Y

(Tian et al., 2011)

Seipin^null has lipid droplet | ectopic phenotype, enhanceable by mino^EY00734/Scer\GAL4^48Y

(Tian et al., 2011)

Seipin^null has fat body phenotype, enhanceable by mdy^QX25

(Tian et al., 2011)

Seipin^null has lipid droplet phenotype, enhanceable by mdy^QX25

(Tian et al., 2011)

Seipin^null has salivary gland phenotype, enhanceable by mdy^EY07280/Scer\GAL4^48Y

(Tian et al., 2011)

Seipin^null has lipid droplet | ectopic phenotype, enhanceable by mdy^EY07280/Scer\GAL4^48Y

(Tian et al., 2011)

Seipin^null has salivary gland phenotype, enhanceable by Cds¹

(Tian et al., 2011)

Seipin^null has lipid droplet | ectopic phenotype, enhanceable by Cds¹

(Tian et al., 2011)

NOT Enhanced by

Statement

Reference

Seipin^null has fat body phenotype, non-enhanceable by Lsd2^KG00149

(Tian et al., 2011)

Seipin^null has lipid droplet phenotype, non-enhanceable by Lsd2^KG00149

(Tian et al., 2011)

Suppressed by

Statement

Reference

Scer\GAL4^ppl.PP, Seipin^null has adult fat body phenotype, suppressible | partially by SERCA^UAS.Tag:MYC, Scer\GAL4^ppl.PP

(Bi et al., 2014)

Scer\GAL4^ppl.PP, Seipin^null has lipid droplet phenotype, suppressible | partially by SERCA^UAS.Tag:MYC, Scer\GAL4^ppl.PP

(Bi et al., 2014)

Scer\GAL4^ppl.PP, Seipin^null has adult fat body phenotype, suppressible | partially by RyR^JF03381, Scer\GAL4^ppl.PP

(Bi et al., 2014)

Scer\GAL4^ppl.PP, Seipin^null has lipid droplet phenotype, suppressible | partially by RyR^JF03381, Scer\GAL4^ppl.PP

(Bi et al., 2014)

Seipin^null has fat body phenotype, suppressible by mdy^EY07280/Scer\GAL4^ppl.PP

(Tian et al., 2011)

Seipin^null has lipid droplet phenotype, suppressible by mdy^EY07280/Scer\GAL4^ppl.PP

(Tian et al., 2011)

Seipin^null has fat body phenotype, suppressible by Scer\GAL4^ppl.PP/SCAP^EY06708

(Tian et al., 2011)

Seipin^null has lipid droplet phenotype, suppressible by Scer\GAL4^ppl.PP/SCAP^EY06708

(Tian et al., 2011)

Seipin^null has salivary gland phenotype, suppressible by mdy^QX25

(Tian et al., 2011)

Seipin^null has lipid droplet | ectopic phenotype, suppressible by mdy^QX25

(Tian et al., 2011)

Seipin^null has salivary gland phenotype, suppressible by Lpin^GD14004/Scer\GAL4^ppl.PP

(Tian et al., 2011)

Seipin^null has lipid droplet | ectopic phenotype, suppressible by Lpin^GD14004/Scer\GAL4^ppl.PP

(Tian et al., 2011)

Seipin^null has salivary gland phenotype, suppressible by Lpin^GD14004/Scer\GAL4^48Y

(Tian et al., 2011)

Seipin^null has lipid droplet | ectopic phenotype, suppressible by Lpin^GD14004/Scer\GAL4^48Y

(Tian et al., 2011)

Seipin^null has salivary gland phenotype, suppressible by Cds^EY08412/Scer\GAL4^48Y

(Tian et al., 2011)

Seipin^null has lipid droplet | ectopic phenotype, suppressible by Cds^EY08412/Scer\GAL4^48Y

(Tian et al., 2011)

Seipin^null has fat body phenotype, suppressible by Scer\GAL4^ppl.PP/Hsap\BSCL2^UAS.cTa

(Tian et al., 2011)

Seipin^null has lipid droplet phenotype, suppressible by Scer\GAL4^ppl.PP/Hsap\BSCL2^UAS.cTa

(Tian et al., 2011)

Seipin^null has salivary gland phenotype, suppressible by Scer\GAL4^ppl.PP/Hsap\BSCL2^UAS.cTa

(Tian et al., 2011)

Seipin^null has lipid droplet | ectopic phenotype, suppressible by Scer\GAL4^ppl.PP/Hsap\BSCL2^UAS.cTa

(Tian et al., 2011)

Seipin^null has fat body phenotype, suppressible by Hsap\BSCL2^S90L.UAS/Scer\GAL4^ppl.PP

(Tian et al., 2011)

Seipin^null has lipid droplet phenotype, suppressible by Hsap\BSCL2^S90L.UAS/Scer\GAL4^ppl.PP

(Tian et al., 2011)

Seipin^null has salivary gland phenotype, suppressible by Hsap\BSCL2^S90L.UAS/Scer\GAL4^ppl.PP

(Tian et al., 2011)

Seipin^null has lipid droplet | ectopic phenotype, suppressible by Hsap\BSCL2^S90L.UAS/Scer\GAL4^ppl.PP

(Tian et al., 2011)

NOT suppressed by

Statement

Reference

Scer\GAL4^ppl.PP, Seipin^null has adult fat body phenotype, non-suppressible by Itpr^GD1676, Scer\GAL4^ppl.PP

(Bi et al., 2014)

Scer\GAL4^ppl.PP, Seipin^null has lipid droplet phenotype, non-suppressible by Itpr^GD1676, Scer\GAL4^ppl.PP

(Bi et al., 2014)

Scer\GAL4^ppl.PP, Seipin^null has adult fat body phenotype, non-suppressible by SERCA^Kum170.UAS, Scer\GAL4^ppl.PP

(Bi et al., 2014)

Scer\GAL4^ppl.PP, Seipin^null has lipid droplet phenotype, non-suppressible by SERCA^Kum170.UAS, Scer\GAL4^ppl.PP

(Bi et al., 2014)

Seipin^null has salivary gland phenotype, non-suppressible by mino^e01407a

(Tian et al., 2011)

Seipin^null has lipid droplet | ectopic phenotype, non-suppressible by mino^e01407a

(Tian et al., 2011)

Seipin^null has salivary gland phenotype, non-suppressible by Agpat2^GD3463/Scer\GAL4^48Y

(Tian et al., 2011)

Seipin^null has lipid droplet | ectopic phenotype, non-suppressible by Agpat2^GD3463/Scer\GAL4^48Y

(Tian et al., 2011)

Seipin^null has salivary gland phenotype, non-suppressible by Agpat1^GD3545/Scer\GAL4^48Y

(Tian et al., 2011)

Seipin^null has lipid droplet | ectopic phenotype, non-suppressible by Agpat1^GD3545/Scer\GAL4^48Y

(Tian et al., 2011)

Seipin^null has salivary gland phenotype, non-suppressible by Agpat2^GD3463/Agpat1^GD3545/Scer\GAL4^48Y

(Tian et al., 2011)

Seipin^null has lipid droplet | ectopic phenotype, non-suppressible by Agpat2^GD3463/Agpat1^GD3545/Scer\GAL4^48Y

(Tian et al., 2011)

Enhancer of

Statement

Reference

Seipin^null is an enhancer of lipid droplet | ectopic phenotype of Cds¹

(Tian et al., 2011)

Seipin^null is an enhancer of fat body phenotype of mdy^QX25

(Tian et al., 2011)

Seipin^null is an enhancer of salivary gland phenotype of Cds¹

(Tian et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Ca-P60A^{Scer\UAS.T:Hsap\MYC} partially rescues the reduction in lipid droplet size seen in Seipin^null mutant fat body cells. The reduction in ionomycin-evoked cytosolic calcium release is also partially rescued.

Expression of Ca-P60A^{Kum170.Scer\UAS} does not rescue the reduction in lipid droplet size seen in Seipin^null mutant fat body cells.

Expression of RyR^JF03381 under the control of Scer\GAL4^ppl.PP significantly rescues the reduction in lipid droplet size and glyceride level seen in Seipin^null mutant fat body cells.

Expression of Itp-r83A^GD1676 under the control of Scer\GAL4^ppl.PP does not rescue the reduction in lipid droplet size seen in Seipin^null mutant fat body cells.

(Bi et al., 2014)

Fat body lipid storage in Seipin^null; Lsd-2^KG00149 animals is similar to Seipin^null single mutants.

mdy^QX25, Seipin^null mutant fat bodies show greatly reduced lipid storage compared to either single mutant.

Expression of either mdy^EY07280 or SCAP^EY06708 using Scer\GAL4^ppl.PP suppresses the reduced lipid storage phenotype seen in Seipin^null fat bodies.

Overexpression of CG5508^EY00734 using Scer\GAL4^48Y enhances the ectopic lipid storage phenotype in salivary glands of Seipin^null mutants.

Overexpression of mdy^EY07280 using Scer\GAL4^48Y in a Seipin^null mutant background results in a massive ectopic lipid storage phenotype in salivary glands.

The ectopic lipid storage phenotype of Seipin^null mutants is fully suppressed by mdy^QX25 or by expression of Lpin^GD14004 using either Scer\GAL4^ppl.PP or Scer\GAL4^48Y.

The ectopic lipid storage phenotype of Seipin^null mutants is not suppressed by CG5508^e01407a or by expression of fu12^GD3463, CG3812^GD3545 or fu12^GD3463 and CG3812^GD3545 together using Scer\GAL4^48Y.

CdsA¹, Seipin^null double mutants exhibit a strong synergistic phenotype (compared to either single mutant) with respect to ectopic lipid droplets in the salivary gland.

The ectopic lipid storage phenotype of Seipin^null mutants is fully suppressed by expressing CdsA^EY08412 using Scer\GAL4^48Y.

There is more phosphatidic acid in the salivary glands of CdsA¹; Seipin^null mutants than in CdsA¹ single mutants.

(Tian et al., 2011)

Xenogenetic Interactions

Statement

Reference

Lipid storage in the fat body is restored and ectopic lipid storage in the salivary gland is reversed in Seipin^null mutants with Hsap\BSCL2^Scer\UAS.cTa or Hsap\BSCL2^{S90L.Scer\UAS} driven by Scer\GAL4^ppl.PP.

(Tian et al., 2011)

Complementation and Rescue Data

Rescued by

Seipin^null is rescued by Scer\GAL4^ppl.PP/Seipin^UAS.Tag:MYC

(Bi et al., 2014)

Seipin^null is rescued by Scer\GAL4^Tub.PU/Seipin^UAS.cTa

(Tian et al., 2011)

Seipin^null is rescued by Scer\GAL4^ppl.PP/Seipin^UAS.cTa

(Tian et al., 2011)

Seipin^null is rescued by Scer\GAL4^Lsp2.PH/Seipin^UAS.cTa

(Tian et al., 2011)

Seipin^null is rescued by Scer\GAL4^Sgs3.PD/Seipin^UAS.cTa

(Tian et al., 2011)

Seipin^null is rescued by Seipin^ΔC.UAS/Scer\GAL4^ppl.PP

(Tian et al., 2011)

Partially rescued by

Seipin^null is partially rescued by Seipin^{ΔN.UAS.Tag:MYC}/Scer\GAL4^ppl.PP

(Bi et al., 2014)

Seipin^null is partially rescued by Seipin^{ΔCT.UAS.Tag:MYC}/Scer\GAL4^ppl.PP

(Bi et al., 2014)

Seipin^null is partially rescued by Scer\GAL4^ppl.PP/Seipin^UAS.cTa

(Tian et al., 2011)

Not rescued by

Seipin^null is not rescued by Scer\GAL4^ppl.PP/Seipin^{ΔLT.UAS.Tag:MYC}

(Bi et al., 2014)

Seipin^null is not rescued by Scer\GAL4^Lsp2.PH/Seipin^UAS.cTa

(Tian et al., 2011)

Seipin^null is not rescued by Scer\GAL4^Sgs3.PD/Seipin^UAS.cTa

(Tian et al., 2011)

Seipin^null is not rescued by Seipin^ΔC.UAS/Scer\GAL4^ppl.PP

(Tian et al., 2011)

Comments

Expression of Seipin^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^ppl.PP rescues the lipid storage phenotype seen in Seipin^null mutant fat bodies.

Expression of Seipin^{ΔN.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^ppl.PP partially rescues the lipid storage phenotype seen in Seipin^null mutant fat bodies.

Expression of Seipin^{ΔCT.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^ppl.PP partially rescues the lipid storage phenotype seen in Seipin^null mutant fat bodies.

Expression of Seipin^{ΔLT.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^ppl.PP does not rescue the lipid storage phenotype seen in Seipin^null mutant fat bodies.

(Bi et al., 2014)

The reduction in total glyceride content of Seipin^null is rescued by expression of Seipin^Scer\UAS.cTa using Scer\GAL4^tub.PU.

The reduction in average weight of Seipin^null flies can be fully rescued by expressing Seipin^Scer\UAS.cTa with Scer\GAL4^tub.PU and partially rescued by expressing Seipin^Scer\UAS.cTa with Scer\GAL4^ppl.PP.

Expression of Seipin^Scer\UAS.cTa using Scer\GAL4^tub.PU reverts the starvation hypersensitivity of Seipin^null flies to control levels.

Expression of Seipin^Scer\UAS.cTa using Scer\GAL4^ppl.PP rescues the lipid storage defects in the fat body and ectopic lipid storage in the salivary gland of Seipin^null mutants.

Expression of Seipin^Scer\UAS.cTa using Scer\GAL4^Lsp2.PH rescues the lipid storage defects in the fat body but not in the salivary gland or in the gut of Seipin^null mutants.

Expression of Seipin^Scer\UAS.cTa using Scer\GAL4^Sgs3.PD rescues the ectopic lipid droplet phenotype in the salivary gland but not the lipid storage defects in the fat body or the ectopic lipid storage in the gut of Seipin^null mutants.

Expression of Seipin^ΔC.Scer\UAS using Scer\GAL4^ppl.PP fully rescues the reduced lipid storage phenotype in the fat body of Seipin^null mutants, but does not rescue the ectopic lipid droplet phenotype in the salivary gland.

(Tian et al., 2011)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Seipin^null

dSeipin

(Bi et al., 2014)

Name Synonyms

Secondary FlyBase IDs

References (2)

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