FlyBase Allele Report: Dmel\Lpin[GD14004]

Fat body cell clones expressing Lpin^GD14004 under the control of Scer\GAL4^tub.PU are smaller in overall size, have smaller nuclei, and contain fewer fat droplets than control clones.

Expression of Lpin^GD14004 under the control of Scer\GAL4^tub.PU in larvae leads to fat body cells displaying an abnormal rounded shape, and highly variable size, with some cells appearing very small with comparatively large nuclei.

Expression of Lpin^GD14004 under the control of Scer\GAL4^Cg.PA in larvae leads to reduced fat droplet size in the fat body, but no effect on fat body cell size or viability of the flies.

(Schmitt et al., 2015)

The wings of Scer\GAL4^ap-md544, Lpin^GD14004 flies show a broadened vein phenotype. In addition, some of these wings also show an extra anterior crossvein phenotype.

(Liu et al., 2011)

Expression under the control of Scer\GAL4¹⁴⁰⁷ results in an increased number and size of GFP-positive aggregates within neuroblast lineage cells in the larval brain compared to controls (these aggregates may indicate cell death or a disturbance of internal cellular structures).

(Neumüller et al., 2011)

Expression of Lpin^GD14004 under the control of Scer\GAL4^αTub84B.PL results in larval lethality, as well as severe lipodistrophy (the amount of fat tissue is reduced).

The size and shape of fat body cells is altered as a result of expression of Lpin^GD14004 under the control of Scer\GAL4^Cg.PA.

The lipid droplets in fat body cells expressing Lpin^GD14004 under the control of Scer\GAL4^Cg.PA are reduced in size compared with those in wild-type controls. This reduction in droplet size is accompanied by a significant overall reduction in triglyceride levels.

Expression of Lpin^GD14004 under the control of Scer\GAL4^DJ761 does not cause lethality. Both male and female flies expressing Lpin^GD14004 under the control of Scer\GAL4^DJ761 show significantly reduced survival under starvation conditions compared with wild-type. Survival of fed flies of this genotype is similar to that of wild-type controls.

Expression of Lpin^GD14004 under the control of Scer\GAL4^DJ649 does not have a significant impact on starvation resistance.

Expression of Lpin^GD14004 via the fat-body specific driver Scer\GAL4^Lsp2.3.1 has a detrimental effect on starvation resistance, resulting in reduced survival under starvation conditions compared with wild-type.

Compared with wild-type controls, expression of Lpin^GD14004 in the adult fat body and gut under the control of RU486-induced Scer\GAL4^Switch1.106 results in significantly reduced survival of the flies under starvation conditions.

(Ugrankar et al., 2011)

Adults expressing Lpin^GD14004 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

14004

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Lpin^GD14004, Scer\GAL4^Cg.PA, raptor^HMS02306 has decreased body size | larval stage phenotype, suppressible by Lpin^UAS.cSa, Scer\GAL4^Cg.PA

(Schmitt et al., 2015)

Lpin^GD14004, Scer\GAL4^Cg.PA, raptor^HMS02306 has decreased body size | larval stage phenotype, suppressible by Lpin^ΔNLS.UAS, Scer\GAL4^Cg.PA

(Schmitt et al., 2015)

Lpin^GD14004, Scer\GAL4^Cg.PA, raptor^HMS02306 has lethal - all die during larval stage phenotype, suppressible by Lpin^UAS.cSa, Scer\GAL4^Cg.PA

(Schmitt et al., 2015)

Lpin^GD14004, Scer\GAL4^Cg.PA, raptor^HMS02306 has lethal - all die during larval stage phenotype, suppressible by Lpin^ΔNLS.UAS, Scer\GAL4^Cg.PA

(Schmitt et al., 2015)

Lpin^GD14004, Scer\GAL4^Cg.PA, raptor^HMS02306 has decreased cell size | larval stage phenotype, suppressible by Lpin^UAS.cSa, Scer\GAL4^Cg.PA

(Schmitt et al., 2015)

Lpin^GD14004, Scer\GAL4^Cg.PA, raptor^HMS02306 has decreased cell size | larval stage phenotype, suppressible by Lpin^ΔNLS.UAS, Scer\GAL4^Cg.PA

(Schmitt et al., 2015)

NOT suppressed by

Statement

Reference

Lpin^GD14004, Scer\GAL4^Cg.PA, raptor^HMS02306 has decreased body size | larval stage phenotype, non-suppressible by Lpin^ΔPAP.UAS, Scer\GAL4^Cg.PA

(Schmitt et al., 2015)

Lpin^GD14004, Scer\GAL4^Cg.PA, raptor^HMS02306 has lethal - all die during larval stage phenotype, non-suppressible by Lpin^ΔPAP.UAS, Scer\GAL4^Cg.PA

(Schmitt et al., 2015)

Lpin^GD14004, Scer\GAL4^Cg.PA, raptor^HMS02306 has decreased cell size | larval stage phenotype, non-suppressible by Lpin^ΔPAP.UAS, Scer\GAL4^Cg.PA

(Schmitt et al., 2015)

Suppressor of

Statement

Reference

Lpin^GD14004/Scer\GAL4^r4 is a suppressor of decreased cell size | larval stage phenotype of Torsin^KO78

(Jacquemyn et al., 2021)

Lpin^GD14004/Scer\GAL4^r4 is a suppressor of decreased size | larval stage phenotype of Torsin^KO78

(Jacquemyn et al., 2021)

Scer\GAL4^arm.PS/Lpin^GD14004 is a suppressor | partially of decreased cell growth rate | larval stage phenotype of Torsin^KO78

(Grillet et al., 2016)

Scer\GAL4^arm.PS/Lpin^GD14004 is a suppressor | partially of partially lethal - majority die phenotype of Torsin^KO78

(Grillet et al., 2016)

NOT Suppressor of

Statement

Reference

Scer\GAL4^arm.PS/Lpin^GD14004 is a non-suppressor of abnormal size | third instar larval stage phenotype of Torsin^KO78

(Grillet et al., 2016)

Other

Statement

Reference

InR^K1409A.UAS, Lpin^GD14004, Scer\GAL4^Cg.PA has increased cell size phenotype

(Schmitt et al., 2015)

Lpin^GD14004, Scer\GAL4^Cg.PA, raptor^HMS02306 has lethal - all die during larval stage phenotype

(Schmitt et al., 2015)

InR^K1409A.UAS, Lpin^GD14004, Scer\GAL4^Cg.PA has lethal - all die before end of P-stage phenotype

(Schmitt et al., 2015)

InR^K1409A.UAS, Lpin^GD14004, Scer\GAL4^Cg.PA has majority die during larval stage phenotype

(Schmitt et al., 2015)

Lpin^GD14004, Scer\GAL4^Cg.PA, raptor^HMS02306 has decreased body size | larval stage phenotype

(Schmitt et al., 2015)

Lpin^GD14004, Scer\GAL4^Cg.PA, raptor^HMS02306 has abnormal size | larval stage phenotype

(Schmitt et al., 2015)

Lpin^GD14004, Scer\GAL4^Cg.PA, raptor^HMS02306 has decreased cell size | larval stage phenotype

(Schmitt et al., 2015)

Phenotype Manifest In

Suppressed by

Statement

Reference

Lpin^GD14004, Scer\GAL4^Cg.PA, raptor^HMS02306 has embryonic/larval fat body | larval stage phenotype, suppressible by Lpin^UAS.cSa, Scer\GAL4^Cg.PA

(Schmitt et al., 2015)

Lpin^GD14004, Scer\GAL4^Cg.PA, raptor^HMS02306 has embryonic/larval fat body | larval stage phenotype, suppressible by Lpin^ΔNLS.UAS, Scer\GAL4^Cg.PA

(Schmitt et al., 2015)

NOT suppressed by

Statement

Reference

Lpin^GD14004, Scer\GAL4^Cg.PA, raptor^HMS02306 has embryonic/larval fat body | larval stage phenotype, non-suppressible by Lpin^ΔPAP.UAS, Scer\GAL4^Cg.PA

(Schmitt et al., 2015)

Suppressor of

Statement

Reference

Lpin^GD14004/Scer\GAL4^r4 is a suppressor | partially of larval fat cell phenotype of Torsin^KO78

(Jacquemyn et al., 2021)

Lpin^GD14004/Scer\GAL4^r4 is a suppressor | partially of nuclear pore | larval stage | decreased number phenotype of Torsin^KO78

(Jacquemyn et al., 2021)

Lpin^GD14004/Scer\GAL4^r4 is a suppressor | partially of nuclear envelope | larval stage phenotype of Torsin^KO78

(Jacquemyn et al., 2021)

Lpin^GD14004/Scer\GAL4^r4 is a suppressor of lipid droplet | increased number | larval stage phenotype of Torsin^KO78

(Jacquemyn et al., 2021)

Lpin^GD14004/Scer\GAL4^r4 is a suppressor | partially of embryonic/larval fat body | larval stage phenotype of Torsin^KO78

(Jacquemyn et al., 2021)

Lpin^GD14004, Scer\GAL4^ppl.PP is a suppressor | partially of embryonic/larval fat body phenotype of Nadk1a^NIG.6145R, Scer\GAL4^ppl.PP

(Xu et al., 2021)

Lpin^GD14004, Scer\GAL4^ppl.PP is a suppressor | partially of mitochondrion | larval stage phenotype of Nadk1a^NIG.6145R, Scer\GAL4^ppl.PP

(Xu et al., 2021)

Lpin^GD14004, Scer\GAL4^ppl.PP is a suppressor | partially of mitochondrion | larval stage phenotype of FASN1^HM05141, Scer\GAL4^ppl.PP

(Xu et al., 2021)

Lpin^GD14004, Scer\GAL4^ppl.PP is a suppressor | partially of embryonic/larval fat body phenotype of FASN1^HM05141, Scer\GAL4^ppl.PP

(Xu et al., 2021)

Lpin^GD14004, Scer\GAL4^ppl.PP is a suppressor of embryonic/larval salivary gland | third instar larval stage phenotype of Pss^KK105709, Scer\GAL4^ppl.PP

(Yang et al., 2019)

Lpin^GD14004, Scer\GAL4^ppl.PP is a suppressor of lipid droplet | increased number phenotype of Pss^KK105709, Scer\GAL4^ppl.PP

(Yang et al., 2019)

Scer\GAL4^arm.PS/Lpin^GD14004 is a suppressor | partially of embryonic/larval fat body | third instar larval stage phenotype of Torsin^KO78

(Grillet et al., 2016)

Lpin^GD14004, Scer\GAL4^71B is a suppressor of wing vein phenotype of Dad^UAS.cTa, Scer\GAL4^71B

(Liu et al., 2011)

Lpin^GD14004/Scer\GAL4^ppl.PP is a suppressor of salivary gland phenotype of Seipin^null

(Tian et al., 2011)

Lpin^GD14004/Scer\GAL4^ppl.PP is a suppressor of lipid droplet | ectopic phenotype of Seipin^null

(Tian et al., 2011)

Lpin^GD14004/Scer\GAL4^48Y is a suppressor of salivary gland phenotype of Seipin^null

(Tian et al., 2011)

Lpin^GD14004/Scer\GAL4^48Y is a suppressor of lipid droplet | ectopic phenotype of Seipin^null

(Tian et al., 2011)

NOT Suppressor of

Statement

Reference

Lpin^GD14004/Scer\GAL4^r4 is a non-suppressor of nuclear membrane | larval stage phenotype of Torsin^KO78

(Jacquemyn et al., 2021)

Other

Statement

Reference

InR^K1409A.UAS, Lpin^GD14004, Scer\GAL4^Cg.PA has nucleus | larval stage phenotype

(Schmitt et al., 2015)

Lpin^GD14004, Scer\GAL4^Cg.PA, raptor^HMS02306 has embryonic/larval fat body | larval stage phenotype

(Schmitt et al., 2015)

InR^K1409A.UAS, Lpin^GD14004, Scer\GAL4^Cg.PA has embryonic/larval fat body | larval stage phenotype

(Schmitt et al., 2015)

InR^K1409A.UAS, Lpin^GD14004, Scer\GAL4^Cg.PA has lipid droplet | larval stage phenotype

(Schmitt et al., 2015)

Lpin^GD14004, Scer\GAL4^Cg.PA, raptor^HMS02306 has nucleus | larval stage phenotype

(Schmitt et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Lpin^GD14004 under the control of Scer\GAL4^arm.PS fails to rescue the reduced fat body size, but partially rescues the growth defect of fat cells, the reduced body weight, and lethality of hemizygous Torsin^KO78 larvae.

(Grillet et al., 2016)

Co-expression of Lpin^GD14004 and raptor^HMS02306 under the control of Scer\GAL4^Cg.PA leads to defective larval growth, decreased larval size, extremely small fat body cells with comparatively large nuclei, and failure to pupariate, unlike expression of either Lpin^GD14004 or raptor^HMS02306 alone.

Co-expression of Lpin^Scer\UAS.cSa or Lpin^{ΔNLS.Scer\UAS}, but not Lpin^{ΔPAP.Scer\UAS}, suppresses the larval growth defects, fat body cell size defects, and inability to pupariate seen in larvae expressing Lpin^GD14004 and raptor^HMS02306 under the control of Scer\GAL4^Cg.PA.

Co-expression of InR^{K1409A.Scer\UAS} and Lpin^GD14004 under the control of Scer\GAL4^Cg.PA leads to a majority of animals dying during larval development, with none reaching adulthood, and larvae exhibit a severely underdeveloped fat body, with greatly enlarged and rounded fat body cells that have enlarged nuclei and contain very small lipid droplets, as compared to controls.

(Schmitt et al., 2015)

Co-expression of Lpin^GD14004 efficiency represses the wing vein phenotype of Scer\GAL4^71B, Dad^Scer\UAS.cTa flies.

(Liu et al., 2011)

The ectopic lipid storage phenotype of Seipin^null mutants is fully suppressed by expression of Lpin^GD14004 using either Scer\GAL4^ppl.PP or Scer\GAL4^48Y.

(Tian et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

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Mutant

Wild-type

Stocks (2)

VDRC

v36006