FlyBase Allele Report: Dmel\Pcyt1[16919]

General Information

Symbol

Dmel\Pcyt1¹⁶⁹¹⁹

Species

D. melanogaster

Name

FlyBase ID

FBal0152682

Feature type

allele

Associated gene

Dmel\Pcyt1

Associated Insertion(s)

P{lacW}Pcyt1¹⁶⁹¹⁹

Carried in Construct

Also Known As

CCT1¹⁶⁹¹⁹

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Weber et al., 2003)

Mutagen

P-element activity

(Weber, 2003.11)

Progenitor genotype

Associated Insertion(s)

P{lacW}Pcyt1¹⁶⁹¹⁹

Cytology

Description

P{lacW}Cct1¹⁶⁹¹⁹ is inserted in the first intron of Cct1.

(Weber et al., 2003)

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3L:1,546,777..1,546,777 [+]

(Gupta and Schupbach, 2003, Weber, 2003.11, Weber et al., 2003)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates congenital generalized lipodystrophy

modeled by Seipin^null

(Tian et al., 2011)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

egg | maternal effect

(Gupta and Schupbach, 2003)

eye photoreceptor cell

(Weber et al., 2003)

eye photoreceptor cell (with Df(3L)Ar14-8)

(Weber et al., 2003)

eye photoreceptor cell (with Pcyt1^EP831)

(Weber et al., 2003)

fat body

(Tian et al., 2011)

lipid droplet

(Tian et al., 2011)

ommatidium

(Weber et al., 2003)

ommatidium (with Df(3L)Ar14-8)

(Weber et al., 2003)

ommatidium (with Pcyt1^EP831)

(Weber et al., 2003)

ommatidium | larval stage | somatic clone

(Weber et al., 2003)

photoreceptor cell R3 | larval stage | somatic clone

(Weber et al., 2003)

photoreceptor cell R4 | larval stage | somatic clone

(Weber et al., 2003)

Detailed Description

Statement

Reference

Cct1¹⁶⁹¹⁹ mutants contain large lipid droplets in the fat body. However, salivary glands of these mutants do not show ectopic lipid storage.

(Tian et al., 2011)

When eggs are laid by mutant females a collapsed egg phenotype is sometimes seen.

(Gupta and Schupbach, 2003)

Cct1¹⁶⁹¹⁹ and Cct1¹⁶⁹¹⁹/Df(3L)Ar14-8 mutants eclose a few days later than wild type. Cct1¹⁶⁹¹⁹ mutants display a mild rough eye phenotype, while Cct1¹⁶⁹¹⁹/Df(3L)Ar14-8 mutants have a more penetrant rough eye phenotype. Such eyes have defects in ommatidial polarity (chirality defects and misrotations) and suffer a loss of photoreceptor cells; quantification of these defects reveals higher numbers in Cct1¹⁶⁹¹⁹/Df(3L)Ar14-8 mutants than Cct1¹⁶⁹¹⁹ mutants. Cct1¹⁶⁹¹⁹/Cct1^EP831 transheterozygotes display a milder rough eye phenotype than Cct1¹⁶⁹¹⁹ homozygotes, with fewer defects scored. Additionally, Cct1¹⁶⁹¹⁹ female mutants lay few eggs and Cct1¹⁶⁹¹⁹/Df(3L)Ar14-8 female mutants lay no eggs, causing sterility in both cases. Mitotic Cct1¹⁶⁹¹⁹ clones in third larval instar eye discs show defects in tissue polarity due to aberrant ommatidial rotation and defects in cell fate selection of the R3/R4 photoreceptor pair leading to abnormal ommatidial chirality.

(Weber et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Pcyt1¹⁶⁹¹⁹ has abnormal planar polarity phenotype, enhanceable by aos^Δ7/argos[+]/N[+]/N^55e11

(Weber et al., 2003)

NOT Enhanced by

Statement

Reference

Pcyt1¹⁶⁹¹⁹ has abnormal planar polarity phenotype, non-enhanceable by aos^Δ7/argos[+]

(Weber et al., 2003)

Pcyt1¹⁶⁹¹⁹ has abnormal planar polarity phenotype, non-enhanceable by argos[+]/aos^W11/N[+]/N^55e11

(Weber et al., 2003)

NOT suppressed by

Statement

Reference

Pcyt1¹⁶⁹¹⁹ has female sterile phenotype, non-suppressible by iPLA2-VIA^Δ23/iPLA2-VIA^Δ23

(Banerjee et al., 2021)

Pcyt1¹⁶⁹¹⁹ has male semi-sterile phenotype, non-suppressible by iPLA2-VIA^Δ23/iPLA2-VIA^Δ23

(Banerjee et al., 2021)

Pcyt1¹⁶⁹¹⁹ has partially lethal phenotype, non-suppressible by iPLA2-VIA^Δ23/iPLA2-VIA^Δ23

(Banerjee et al., 2021)

Phenotype Manifest In

Enhanced by

Statement

Reference

Pcyt1¹⁶⁹¹⁹ has eye photoreceptor cell phenotype, enhanceable by aos^Δ7/argos[+]/N[+]/N^55e11

(Weber et al., 2003)

Pcyt1¹⁶⁹¹⁹ has ommatidium phenotype, enhanceable by aos^Δ7/argos[+]/N[+]/N^55e11

(Weber et al., 2003)

Pcyt1¹⁶⁹¹⁹ has eye phenotype, enhanceable by N[+]/N^55e11

(Weber et al., 2003)

NOT Enhanced by

Statement

Reference

Pcyt1¹⁶⁹¹⁹ has eye photoreceptor cell phenotype, non-enhanceable by aos^Δ7/argos[+]

(Weber et al., 2003)

Pcyt1¹⁶⁹¹⁹ has ommatidium phenotype, non-enhanceable by aos^Δ7/argos[+]

(Weber et al., 2003)

Pcyt1¹⁶⁹¹⁹ has eye photoreceptor cell phenotype, non-enhanceable by argos[+]/aos^W11/N[+]/N^55e11

(Weber et al., 2003)

Pcyt1¹⁶⁹¹⁹ has ommatidium phenotype, non-enhanceable by argos[+]/aos^W11/N[+]/N^55e11

(Weber et al., 2003)

Enhancer of

Statement

Reference

Pcyt1¹⁶⁹¹⁹/Cct1[+] is an enhancer of wing phenotype of N^55e11

(Weber et al., 2003)

Suppressor of

Statement

Reference

Pcyt1¹⁶⁹¹⁹/Cct1[+] is a suppressor of crossvein | increased number phenotype of Egfr^E3

(Weber et al., 2003)

Pcyt1¹⁶⁹¹⁹/Cct1[+] is a suppressor of eye photoreceptor cell phenotype of Egfr^E3

(Weber et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

Egfr^E3/+, Cct1¹⁶⁹¹⁹/+ double mutants show reduced loss of photoreceptors in the eye, a more regular arrangement of ommatidia and a reduction in the presence of extra crossveins in the wing compared to Egfr^E3/+ single mutants. The Cct1¹⁶⁹¹⁹ allele dominantly enhances the notched wing phenotype seen in N^55e11/+ flies. While Cct1¹⁶⁹¹⁹/+, argos^Δ7/+ double heterozygotes have no eye defects, N^55e11/+; Cct1¹⁶⁹¹⁹/+, argos^Δ7/+ triple heterozygotes show an enhancement in the defective eye phenotype (loss of photoreceptor cells, defects in ommatidial polarity and chirality and a partial loss of the ventral eye) seen in Cct1¹⁶⁹¹⁹ mutants. This phenotype is not significantly enhanced in N^55e11/+; Cct1¹⁶⁹¹⁹/+, argos^W11/+ triple heterozygotes. The Cct1¹⁶⁹¹⁹ homozygous phenotype is strongly enhanced in N^55e11/+ background mutants causing severely reduced eyes.

(Weber et al., 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Pcyt1¹⁶⁹¹⁹ is rescued by Pcyt1^+t11.2

(Weber et al., 2003)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

7319

w^*; P{lacW}Pcyt1¹⁶⁹¹⁹/TM6B, Tb⁺

Notes on Origin

Discoverer

Isolated in screen described in FBrf0083893.

(Weber, 2003.11)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

CCT1¹⁶⁹¹⁹

(Weber et al., 2003)

Cct1¹⁶⁹¹⁹

(Tian et al., 2011)

Pcyt1¹⁶⁹¹⁹

pcyt1¹⁶⁹¹⁹

(Banerjee et al., 2021)

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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