Lsd-2KG00149 knockdown leads to a reduction in phase IIa inward shift of lipid droplets.
Lipid droplets in the larval fat body are smaller than normal in mutant animals. The total glyceride level is reduced compared to wild type.
Lsd-2KG00149 mutants have no ectopic lipid storage in salivary glands.
The net plus-end (phase II) transport of lipid droplets along microtubules is disrupted in Lsd-2KG00149 embryos; lipid droplets are distributed throughout the periphery in the mutants during this phase, while droplets are localized basally in wild-type embryos. Quantification of droplet motion shows that both plus-end and minus-end periods of uninterrupted motion are slightly decreased in relation to the wild type in phase II, but the decrease in plus-end motion is larger, resulting in abolishment of net transport. For both directions, motion is slightly slower in the mutants than in wild-type embryos. The parameters of droplet motion in phase II and phase III (net minus-end transport) are essentially the same in Lsd-2KG00149 embryos. Lsd-2KG00149/+ embryos only show this phenotype when the Lsd-2KG00149 allele is inherited from the mother. Embryonic development does not appear to be affected in Lsd-2KG00149 mutants and the distribution of other organelles is not affected. Many embryos hatch and give rise to fertile adults.
Mutants are lean, containing significantly lower levels of triacylglycerols (37.2% of controls).