FlyBase Recombinant Construct Report: P{SUPor-P}

General Information

Symbol

P{SUPor-P}

FlyBase ID

FBtp0001587

Feature type

transgenic_transposable_element

Component Allele(s)

gypsy\su(Hw)BR⁴³⁰

w^BR.E.BR

Size

11.467

Expression Data

Associated insertion(s)

2568 available

Molecular map

Description and Uses

Location-dependent role

Description

CV term

Qualifiers and info

Reference

transposon

P-element

(Bellen et al., 2004, Parks et al., 2004, Roseman et al., 1995)

Construct components

Product class / Tool use(s)

Marker(s)

y^+mDint2

Description

A diagram of the P{SUPor-P} element is available at the [GDP Transposons page](http://flypush.imgen.bcm.tmc.edu/pscreen/transposons.html)

(FlyBase, 1992-)

Component allele gypsy\su(Hw)BR⁴³⁰

Description

Contains 12 su(Hw)-binding sites, corresponding to bp 647-1077 in the gypsy transposon.

(Kuhn et al., 2003)

Construct: Consists of a 430bp gypsy\su(Hw)BR, including all 12 binding sites.

(Patton et al., 1992)

Consists of a 430bp gypsy\su(Hw)BR, including all 12 binding sites, nucleotides 647 to 1077 of FBrf0045014.

(Roseman et al., 1993)

Consists of a 430bp gypsy\su(Hw)BR, gypsy sequences 647-1077 (coordinates of FBrf0045014).

(Roseman et al., 1995)

Component allele w^BR.E.BR

Description

P{SUPor-P-lt} contains a mini-w gene that has two flanking gypsy\su(Hw)BR sites, one of which is upstream of the w eye promoter.

(Bhadra et al., 1999)

Construct: w^+mC minigene altered to include a su(Hw) binding region (all 12 binding sites, nucleotides 647 to 1077 of Marlor et al., Mol Cell Biol 6:1129-1134 ), upstream of the w eye enhancer (nucleotides -1084 to -1465 of Qian et al., Genetics 131:79-90 ), upstream of the w coding region, upstream of a second su(Hw) binding region.

(Roseman et al., 1993)

Two gypsy\su(Hw)BR binding sites flank the w^+mET sequences to insulate the w gene from chromosomal position effects.

(Roseman et al., 1995)

Sequence Data

Sequence (FB)

FlyBase-compiled

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA Sequence

Extent

Segments and Size

Total Size

11.467

Left end

P5\'

(Roseman et al., 1993)

Right end

P3\'

(Roseman et al., 1993)

Segments

Num

Orientation

Symbol

Reference

(Roseman et al., 1993)

Features

CV term

Qualifiers and info

Reference

regulatory/structural

DNA

(Kurenova et al., 1998, Mallin et al., 1998, Roseman et al., 1995, Roseman et al., 1993)

selectable

marker

(Roseman et al., 1993)

selectable

marker

(Mallin et al., 1998, Roseman et al., 1995, Roseman et al., 1993, Patton et al., 1992)

structural

element

(Roseman et al., 1995)

Component Alleles

Allele

gypsy\su(Hw)BR⁴³⁰

Reference(s)

(Lankenau et al., 2000, Kurenova et al., 1998, Mallin et al., 1998, Roseman et al., 1995, Roseman et al., 1993)

Molecular data

Contains 12 su(Hw)-binding sites, corresponding to bp 647-1077 in the gypsy transposon.

(Kuhn et al., 2003)

Consists of a 430bp gypsy\su(Hw)BR, gypsy sequences 647-1077 (coordinates of FBrf0045014).

(Roseman et al., 1995)

Consists of a 430bp gypsy\su(Hw)BR, including all 12 binding sites, nucleotides 647 to 1077 of FBrf0045014.

(Roseman et al., 1993)

Construct: Consists of a 430bp gypsy\su(Hw)BR, including all 12 binding sites.

(Patton et al., 1992)

Phenotype Data

Phenotypic Class Phenotype Manifest In Detailed Description

Allele

w^BR.E.BR

Reference(s)

(Maggert and Golic, 2002, Roseman et al., 2001, Lankenau et al., 2000, Bhadra et al., 1999, Soldatov et al., 1999, Kurenova et al., 1998, Mallin et al., 1998, Roseman et al., 1995, Roseman et al., 1993)

Molecular data

P{SUPor-P-lt} contains a mini-w gene that has two flanking gypsy\su(Hw)BR sites, one of which is upstream of the w eye promoter.

(Bhadra et al., 1999)

Two gypsy\su(Hw)BR binding sites flank the w^+mET sequences to insulate the w gene from chromosomal position effects.

(Roseman et al., 1995)

(Roseman et al., 1993)

Phenotype Data

Phenotypic Class Phenotype Manifest In Detailed Description

Expression Data

Reporter Expression

Additional Information

Statement