FlyBase Allele Report: Dmel\Lsd-1[38]

General Information

Symbol

Dmel\Lsd-1³⁸

Species

D. melanogaster

Name

FlyBase ID

FBal0280209

Feature type

allele

Associated gene

Dmel\Lsd-1

Associated Insertion(s)

Mi{?ET1}Lsd-1³⁸

Carried in Construct

Also Known As

plin1³⁸

Key Links

Allele class

amorphic allele - molecular evidence

Mutagen

minos activity

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Bi et al., 2012)

Mutagen

minos activity

(Bi et al., 2012)

Progenitor genotype

Mi{ET1}Lsd-1^MB10034

(Bi et al., 2012)

Associated Insertion(s)

Mi{?ET1}Lsd-1³⁸

Cytology

Description

Imprecise excision of the progenitor insertion, with approximately 2kb of the original element remaining. In addition, there is a perfect deletion of intron 3, exon 4 and intron 4 of the Lsd-1-RA transcript (which encodes the longest protein isoform). This deletion is 1kb away from the insertion site.

(Bi et al., 2012)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal immune response | adult stage

(Wang et al., 2021)

abnormal size | larval stage

(Yao et al., 2018)

abnormal starvation stress response | larval stage

(Bi et al., 2012)

long lived | conditional

(Wang et al., 2021)

Phenotype Manifest In

Detailed Description

Statement

Reference

Lsd-1³⁸ adults show large lipid droplets in the fat body. Lsd-1³⁸ adults are resistant to infection with S. typhimurium (decreased mortality and decreased bacterial load).

(Wang et al., 2021)

Lsd-1³⁸ mutants present significantly bigger lipid droplets in the larval fat body, and isolated lipid droplet coalesce more frequently, as compared to controls.

(Yao et al., 2018)

Mutant larvae have larger lipid droplets in the fat body than wild-type larvae, under both fed and starvation conditions.

Total glyceride levels in mutant wandering stage third-instar larvae are normal under fed conditions. Under starvation conditions, the total glyceride level barely decreases in mutant larvae compared to fed conditions and is significantly higher than that in wild-type starved larvae. The level of circulating lipid in the hemolymph is reduced in mutant larvae compared to controls under fed conditions.

Homozygous larvae show reduced survival compared to controls under starvation conditions.

(Bi et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

Lsd-1³⁸ has embryonic/larval fat body | larval stage phenotype, enhanceable by Hsl^b24

(Bi et al., 2012)

Lsd-1³⁸ has lipid droplet phenotype, enhanceable by Hsl^b24

(Bi et al., 2012)

Lsd-1³⁸ has embryonic/larval fat body | larval stage phenotype, enhanceable by bmm¹

(Bi et al., 2012)

Lsd-1³⁸ has lipid droplet phenotype, enhanceable by bmm¹

(Bi et al., 2012)

Lsd-1³⁸ has embryonic/larval fat body | larval stage phenotype, enhanceable by Lsd-2^UAS.cGa/Scer\GAL4^ppl.PP

(Bi et al., 2012)

Lsd-1³⁸ has lipid droplet phenotype, enhanceable by Lsd-2^UAS.cGa/Scer\GAL4^ppl.PP

(Bi et al., 2012)

Lsd-1³⁸ has embryonic/larval fat body | larval stage phenotype, enhanceable by Lsd-2^UAS.EYFP/Scer\GAL4^ppl.PP

(Bi et al., 2012)

Lsd-1³⁸ has lipid droplet phenotype, enhanceable by Lsd-2^UAS.EYFP/Scer\GAL4^ppl.PP

(Bi et al., 2012)

Suppressed by

Statement

Reference

Lsd-1³⁸ has embryonic/larval fat body | larval stage phenotype, suppressible by Lsd-1::Lsd-2^{2.1C.UAS.mCherry}/Scer\GAL4^ppl.PP

(Bi et al., 2012)

Lsd-1³⁸ has lipid droplet phenotype, suppressible by Lsd-1::Lsd-2^{2.1C.UAS.mCherry}/Scer\GAL4^ppl.PP

(Bi et al., 2012)

Enhancer of

Statement

Reference

Lsd-1³⁸ is an enhancer of lipid droplet phenotype of Lsd-2^KG00149

(Bi et al., 2012)

Lsd-1³⁸ is an enhancer of embryonic/larval fat body | larval stage phenotype of Lsd-2^KG00149

(Bi et al., 2012)

NOT Suppressor of

Statement

Reference

Lsd-1³⁸ is a non-suppressor of mesothoracic tergotrochanter muscle cell | progressive phenotype of HDAC6^KO

(Yan et al., 2017)

Lsd-1³⁸ is a non-suppressor of lipid droplet | adult stage | progressive phenotype of HDAC6^KO

(Yan et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

The large lipid droplet phenotype seen in Lsd-1³⁸ larvae is slightly enhanced by Hsl^b24.

The large lipid droplet phenotype seen in Lsd-1³⁸ larvae is enhanced by bmm¹.

Expression of either Lsd-2^Scer\UAS.cGa or Lsd-2^{Scer\UAS.T:Avic\GFP-EYFP} under the control of Scer\GAL4^ppl.PP enhances the large lipid droplet phenotype seen in Lsd-1³⁸ larvae.

The reduction in size of the lipid droplets and the reduction in total glyceride level is even more severe in Lsd-1³⁸ Lsd-2^KG00149 double mutant larvae than in Lsd-2^KG00149 single mutants.

Expression of Lsd-1::Lsd-2^{2.1C.Scer\UAS.T:Disc\RFP-mCherry} under the control of Scer\GAL4^ppl.PP rescues the large lipid droplet phenotype seen in Lsd-1³⁸ larvae.

(Bi et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Lsd-1³⁸ is rescued by Scer\GAL4^ppl.PP/Lsd-1^UAS.mCherry

(Bi et al., 2012)

Not rescued by

Lsd-1³⁸ is not rescued by Scer\GAL4^ppl.PP/Lsd-1^ΔC.UAS.EYFP

(Bi et al., 2012)

Comments

The reduced survival of Lsd-1³⁸ larvae compared to controls under starvation conditions is rescued by expression of Lsd-1^{Scer\UAS.T:Disc\RFP-mCherry} under the control of Scer\GAL4^ppl.PP.

Expression of Lsd-1^{Scer\UAS.T:Disc\RFP-mCherry} under the control of Scer\GAL4^ppl.PP cell-autonomously rescues the large lipid droplet phenotype of Lsd-1³⁸ larvae.

Expression of Lsd-1^{ΔC.Scer\UAS.T:Avic\GFP-EYFP} under the control of Scer\GAL4^ppl.PP does not rescue the large lipid droplet phenotype seen in Lsd-1³⁸ larvae.

(Bi et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Lsd-1³⁸

plin1³⁸

(Wang et al., 2021, Harsh et al., 2019, Yao et al., 2018, Yan et al., 2017, Bi et al., 2012)

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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