FlyBase Allele Report: Dmel\Lsd-2[UAS.cGa]

General Information

Symbol

Dmel\Lsd-2^UAS.cGa

Species

D. melanogaster

Name

FlyBase ID

FBal0147803

Feature type

allele

Associated gene

Dmel\Lsd-2

Associated Insertion(s)

Carried in Construct

P{UAS-Lsd-2.G}

Also Known As

UAS-Lsd2

Key Links

Transgenic product class

cDNA

(Gronke, 2003)

wild_type

(Gronke, 2003)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Gronke, 2003)

wild_type

(Gronke, 2003)

Mutagen

in vitro construct

(Gronke, 2003, Gronke et al., 2003)

Progenitor genotype

Carried in construct

P{UAS-Lsd-2.G}

Cytology

Description

UAS sequences drive expression of a Lsd-2 cDNA.

(Gronke, 2003)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Lsd-2

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of obesity

CEA

(Gronke et al., 2003)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates neurodegeneration with brain iron accumulation 4

modeled by Nazo^KO

(Sreejith et al., 2024)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

embryonic/larval oenocyte | larval stage | nutrition conditional, with Scer\GAL4^Desat1.PB

(Cinnamon et al., 2016)

fat body, with Scer\GAL4^Adh.PF

(Gronke et al., 2003)

fat body, with Scer\GAL4^fat

(Gronke et al., 2003)

lipid droplet & larval oenocyte, with Scer\GAL4^ppl.PP

(Gutierrez et al., 2007)

Detailed Description

Statement

Reference

Expression of Lsd-2^Scer\UAS.cGa under the control of Scer\GAL4^Desat1.PB results in a significant increase in lipid droplets in larval oenocytes in fed, but not starved, larvae, and does not change oenocyte size in starved conditions, as compared to controls.

(Cinnamon et al., 2016)

Expression of Lsd-2^Scer\UAS.cGa under the control of Scer\GAL4^ppl.PP reduces the accumulation of lipid droplets in 78% of starved larvae, with approximately 4-fold fewer large droplets per oenocyte.

(Gutierrez et al., 2007)

Immature adult flies expressing Lsd-2^Scer\UAS.cGa under the control of Scer\GAL4^fat have more storage fat (measured as triacylglycerol content) than control flies.

(Gronke et al., 2005)

Lsd-2^Scer\UAS.cGa driven by Scer\GAL4^fat causes obesity. Triacylglycerol levels are elevated bu 28%. Flies are also more starvation resistant than controls. When Lsd-2^Scer\UAS.cGa is driven by Scer\GAL4^Adh.PF triacylglycerol levels increase by 48.5%.

(Gronke et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhancer of

Statement

Reference

Lsd-2^UAS.cGa/Scer\GAL4^ppl.PP is an enhancer of embryonic/larval fat body | larval stage phenotype of Lsd-1³⁸

(Bi et al., 2012)

Lsd-2^UAS.cGa/Scer\GAL4^ppl.PP is an enhancer of lipid droplet phenotype of Lsd-1³⁸

(Bi et al., 2012)

Suppressor of

Statement

Reference

Lsd-2^UAS.cGa/Scer\GAL4^NP0001 is a suppressor of lipid droplet | adult stage phenotype of CG3740^CRISPR, Nazo^RNAi.UAS.cSa, Scer\GAL4^Act5C.PI

(Sreejith et al., 2024)

Lsd-2^UAS.cGa/Scer\GAL4^NP0001 is a suppressor of adult midgut phenotype of CG3740^CRISPR, Nazo^RNAi.UAS.cSa, Scer\GAL4^Act5C.PI

(Sreejith et al., 2024)

Lsd-2^UAS.cGa, Scer\GAL4^NP0001 is a suppressor of lipid droplet | adult stage phenotype of Nazo^RNAi.UAS.cSa, Scer\GAL4^NP0001

(Sreejith et al., 2024)

Lsd-2^UAS.cGa, Scer\GAL4^NP0001 is a suppressor of adult midgut phenotype of Nazo^RNAi.UAS.cSa, Scer\GAL4^NP0001

(Sreejith et al., 2024)

Lsd-2^UAS.cGa, Scer\GAL4^fat is a suppressor | partially of phenotype of Scer\GAL4^fat, bmm^UAS.cGa

(Gronke et al., 2005)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Lsd-2^Scer\UAS.cGa under the control of Scer\GAL4^ppl.PP enhances the large lipid droplet phenotype seen in Lsd-1³⁸ larvae.

(Bi et al., 2012)

Immature adult flies co-expressing Lsd-2^Scer\UAS.cGa and bmm^Scer\UAS.cGa under the control of Scer\GAL4^fat have storage fat levels (measured as triacylglycerol content) intermediate between control flies and flies expressing bmm^Scer\UAS.cGa alone.

(Gronke et al., 2005)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^fat/Lsd-2^UAS.cGa rescues Lsd-2^KG00149

(Gronke et al., 2003)

Comments

Lsd-2^Scer\UAS.cGa driven by Scer\GAL4^fat rescues the leanness phenotype seen in these animals.

(Gronke et al., 2003)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

98116

w^*; P{UAS-Lsd-2.G}2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Lsd-2^Scer\UAS.cGa

Lsd-2^UAS.cGa

Name Synonyms

Secondary FlyBase IDs

References (10)

Report Sections

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