FlyBase Allele Report: Dmel\Nazo[RNAi.UAS.cSa]

General Information

Symbol

Dmel\Nazo^RNAi.UAS.cSa

Species

D. melanogaster

Name

FlyBase ID

FBal0399914

Feature type

allele

Associated gene

Dmel\Nazo

Associated Insertion(s)

Carried in Construct

P{UAS-Nazo.RNAi.S}

Key Links

Transgenic product class

RNAi_reagent

(Sreejith et al., 2024)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Sreejith et al., 2024)

Mutagen

in vitro construct

(Sreejith et al., 2024)

Progenitor genotype

Carried in construct

P{UAS-Nazo.RNAi.S}

Cytology

Description

UAS sequences regulate expression of two miRNAs that each target a different part of Nazo (targeted sequences are TTCAGCCATCTCAGAGATAATC and GGTCATTTTGAACGATTTGACG).

(Sreejith et al., 2024)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Nazo

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of neurodegeneration with brain iron accumulation 4

CEA

(Sreejith et al., 2024)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult fat body, with Scer\GAL4^Act5C.PI

(Sreejith et al., 2024)

adult midgut, with Scer\GAL4^Act5C.PI

(Sreejith et al., 2024)

adult midgut, with Scer\GAL4^NP0001

(Sreejith et al., 2024)

lipid droplet | adult stage, with Scer\GAL4^Act5C.PI

(Sreejith et al., 2024)

lipid droplet | adult stage, with Scer\GAL4^NP0001

(Sreejith et al., 2024)

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressed by

Statement

Reference

Nazo^RNAi.UAS.cSa, Scer\GAL4^NP0001 has lipid droplet | adult stage phenotype, suppressible by Lsd2^UAS.cGa, Scer\GAL4^NP0001

(Sreejith et al., 2024)

Nazo^RNAi.UAS.cSa, Scer\GAL4^NP0001 has adult midgut phenotype, suppressible by Lsd2^UAS.cGa, Scer\GAL4^NP0001

(Sreejith et al., 2024)

CG3740^CRISPR, Nazo^RNAi.UAS.cSa, Scer\GAL4^Act5C.PI has lipid droplet | adult stage phenotype, suppressible by Scer\GAL4^NP0001/bmm^JF01946

(Sreejith et al., 2024)

CG3740^CRISPR, Nazo^RNAi.UAS.cSa, Scer\GAL4^Act5C.PI has adult midgut phenotype, suppressible by Scer\GAL4^NP0001/bmm^JF01946

(Sreejith et al., 2024)

CG3740^CRISPR, Nazo^RNAi.UAS.cSa, Scer\GAL4^Act5C.PI has lipid droplet | adult stage phenotype, suppressible by Lsd2^UAS.cGa/Scer\GAL4^NP0001

(Sreejith et al., 2024)

CG3740^CRISPR, Nazo^RNAi.UAS.cSa, Scer\GAL4^Act5C.PI has adult midgut phenotype, suppressible by Lsd2^UAS.cGa/Scer\GAL4^NP0001

(Sreejith et al., 2024)

Nazo^RNAi.UAS.cSa, Scer\GAL4^NP0001 has lipid droplet | adult stage phenotype, suppressible by bmm^JF01946, Scer\GAL4^NP0001

(Sreejith et al., 2024)

Nazo^RNAi.UAS.cSa, Scer\GAL4^NP0001 has adult midgut phenotype, suppressible by bmm^JF01946, Scer\GAL4^NP0001

(Sreejith et al., 2024)

NOT suppressed by

Statement

Reference

CG3740^CRISPR, Nazo^RNAi.UAS.cSa, Scer\GAL4^Act5C.PI has lipid droplet | adult stage phenotype, non-suppressible by dob^HMC06191, Scer\GAL4^Act5C.PI

(Sreejith et al., 2024)

CG3740^CRISPR, Nazo^RNAi.UAS.cSa, Scer\GAL4^Act5C.PI has adult midgut phenotype, non-suppressible by dob^HMC06191, Scer\GAL4^Act5C.PI

(Sreejith et al., 2024)

CG3740^CRISPR, Nazo^RNAi.UAS.cSa, Scer\GAL4^Act5C.PI has lipid droplet | adult stage phenotype, non-suppressible by sturkopf^UAS.EGFP, Scer\GAL4^Act5C.PI

(Sreejith et al., 2024)

CG3740^CRISPR, Nazo^RNAi.UAS.cSa, Scer\GAL4^Act5C.PI has adult midgut phenotype, non-suppressible by sturkopf^UAS.EGFP, Scer\GAL4^Act5C.PI

(Sreejith et al., 2024)

Other

Statement

Reference

CG3740^CRISPR, Nazo^RNAi.UAS.cSa, Scer\GAL4^Act5C.PI has adult midgut phenotype

(Sreejith et al., 2024)

CG3740^CRISPR, Nazo^RNAi.UAS.cSa, Scer\GAL4^Act5C.PI has lipid droplet | adult stage phenotype

(Sreejith et al., 2024)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Nazo^RNAi.UAS.cSa is rescued by Scer\GAL4^NP0001/Nazo^UAS.Tag:MYC

(Sreejith et al., 2024)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Nazo^RNAi.UAS.cSa

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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