FlyBase Allele Report: Mmus\Mdm2[UAS.cFa]

General Information

Symbol

Mmus\Mdm2^UAS.cFa

Species

M. musculus

Name

Saccharomyces cerevisiae UAS construct a of Folberg-Blum

FlyBase ID

FBal0269357

Feature type

allele

Associated gene

Mmus\Mdm2

Associated Insertion(s)

Carried in Construct

P{UAS-Mdm2.F}

Transgenic product class

cDNA

(Folberg-Blum et al., 2002)

wild_type

(Folberg-Blum et al., 2002)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Folberg-Blum et al., 2002)

wild_type

(Folberg-Blum et al., 2002)

Mutagen

in vitro construct

(Folberg-Blum et al., 2002)

Progenitor genotype

Carried in construct

P{UAS-Mdm2.F}

Cytology

Description

UASt regulatory sequences drive expression of a Mmus\Mdm2 cDNA.

(Folberg-Blum et al., 2002)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Mmus\Mdm2

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size | adult stage, with Scer\GAL4^GMR.PF

(Folberg-Blum et al., 2002)

increased cell death | third instar larval stage, with Scer\GAL4^Bx-MS1096

(Folberg-Blum et al., 2002)

visible, with Scer\GAL4^Bx-MS1096

(Folberg-Blum et al., 2002)

visible, with Scer\GAL4^GMR.PF

(Folberg-Blum et al., 2002)

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PF

(Folberg-Blum et al., 2002)

interommatidial bristle, with Scer\GAL4^GMR.PF

(Folberg-Blum et al., 2002)

ommatidium, with Scer\GAL4^GMR.PF

(Folberg-Blum et al., 2002)

wing, with Scer\GAL4^Bx-MS1096

(Folberg-Blum et al., 2002)

wing disc | third instar larval stage, with Scer\GAL4^Bx-MS1096

(Folberg-Blum et al., 2002)

Detailed Description

Statement

Reference

Expression of Mmus\Mdm2^Scer\UAS.cFa under the control of Scer\GAL4^Bx-MS1096 results in wing defects which differ in severity, depending on the insertion line used: either a weaker, blistered phenotype or a stronger, gnarled phenotype is seen. There is a dramatic increase in the number of apoptotic cells in the wing disc in mutant third instar larvae, while the number of proliferating cells appears normal.

Expression of Mmus\Mdm2^Scer\UAS.cFa under the control of Scer\GAL4^GMR.PF results in eye defects which differ in severity, depending on the insertion line used: either rough or small eyes are produced. In flies with rough eyes, disorganisation of the interommatidial bristles and some ommatidial fusion is seen. In flies with small eyes, there is complete disorganisation of the interommatidial bristles and ommatidia are hardly visible. The level of apoptosis and cell proliferation in the eye disc appears normal in mutant third instar larvae.

(Folberg-Blum et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

Mmus\Mdm2^UAS.cFa, Scer\GAL4^GMR.PF has visible phenotype, non-suppressible by E2f1^GMR.PD

(Folberg-Blum et al., 2002)

Mmus\Mdm2^UAS.cFa, Scer\GAL4^GMR.PF has abnormal size | adult stage phenotype, non-suppressible by E2f1^GMR.PD

(Folberg-Blum et al., 2002)

Mmus\Mdm2^UAS.cFa, Scer\GAL4^GMR.PF has visible phenotype, non-suppressible by BacA\p35^UAS.cHa, Scer\GAL4^GMR.PF

(Folberg-Blum et al., 2002)

Mmus\Mdm2^UAS.cFa, Scer\GAL4^GMR.PF has visible phenotype, non-suppressible by Akt⁰⁴²²⁶

(Folberg-Blum et al., 2002)

Mmus\Mdm2^UAS.cFa, Scer\GAL4^GMR.PF has abnormal size | adult stage phenotype, non-suppressible by BacA\p35^UAS.cHa

(Folberg-Blum et al., 2002)

Mmus\Mdm2^UAS.cFa, Scer\GAL4^GMR.PF has abnormal size | adult stage phenotype, non-suppressible by Akt⁰⁴²²⁶

(Folberg-Blum et al., 2002)

Mmus\Mdm2^UAS.cFa, Scer\GAL4^GMR.PF has visible phenotype, non-suppressible by E2f1⁰⁷¹⁷²

(Folberg-Blum et al., 2002)

Mmus\Mdm2^UAS.cFa, Scer\GAL4^GMR.PF has abnormal size | adult stage phenotype, non-suppressible by E2f1⁰⁷¹⁷²

(Folberg-Blum et al., 2002)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

Mmus\Mdm2^UAS.cFa, Scer\GAL4^GMR.PF has eye phenotype, non-suppressible by BacA\p35^UAS.cHa, Scer\GAL4^GMR.PF

(Folberg-Blum et al., 2002)

Mmus\Mdm2^UAS.cFa, Scer\GAL4^GMR.PF has eye phenotype, non-suppressible by Akt⁰⁴²²⁶

(Folberg-Blum et al., 2002)

Mmus\Mdm2^UAS.cFa, Scer\GAL4^GMR.PF has eye phenotype, non-suppressible by E2f1⁰⁷¹⁷²

(Folberg-Blum et al., 2002)

Mmus\Mdm2^UAS.cFa, Scer\GAL4^GMR.PF has eye phenotype, non-suppressible by E2f1^GMR.PD

(Folberg-Blum et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Co-expression of BacA\p35^Scer\UAS.cHa or E2f^GMR.PD does not rescue the eye phenotype caused by expression of Mmus\Mdm2^Scer\UAS.cFa under the control of Scer\GAL4^GMR.PF.

The eye phenotype caused by expression of Mmus\Mdm2^Scer\UAS.cFa under the control of Scer\GAL4^GMR.PF is not rescued if the flies are also carrying either Akt1⁰⁴²²⁶ or E2f⁰⁷¹⁷².

(Folberg-Blum et al., 2002)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Mmus\Mdm2^Scer\UAS.cFa

Mmus\Mdm2^UAS.cFa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Folberg-Blum

Secondary FlyBase IDs

References (1)

Report Sections

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