FlyBase Allele Report: Dmel\Akt[UAS.cSa]

General Information

Symbol

Dmel\Akt^UAS.cSa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Staveley

FlyBase ID

FBal0089843

Feature type

allele

Associated gene

Dmel\Akt

Associated Insertion(s)

Carried in Construct

P{UAS-Akt.S}

Also Known As

UAS-Akt1, UAS-akt

Key Links

Transgenic product class

cDNA

(Staveley et al., 1998)

wild_type

(Staveley et al., 1998)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Staveley et al., 1998)

wild_type

(Staveley et al., 1998)

Mutagen

in vitro construct

(Staveley et al., 1998)

Progenitor genotype

Carried in construct

P{UAS-Akt.S}

Cytology

Description

UASt regulatory sequences drive expression of a Akt1 cDNA.

(Staveley et al., 1998)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Akt

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible, with Scer\GAL4^GMR.PF

(Ramel et al., 2007)

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PF

(Tsuda et al., 2010, Ramel et al., 2007)

ommatidium, with Scer\GAL4^GMR.PF

(Kramer et al., 2003)

Detailed Description

Statement

Reference

Ectopic expression of Akt1^Scer\UAS.cSa in the eye imaginal disc under the control of Scer\GAL4^GMR.PF increases the size of compound eyes.

(Tsuda et al., 2010)

Expression of Akt1^Scer\UAS.cSa under the control of Scer\GAL4^GMR.PF results in large eyes with larger ommatidia than normal.

(Ramel et al., 2007)

Pupae expressing Akt1^Scer\UAS.cSa under the control of Scer\GAL4^hs.PB (using a 30 minute heat shock at 9 hours after puparium formation) do not have intact salivary glands at 20 hours after puparium formation (as occurs in wild-type animals).

(Liu and Lehmann, 2006)

Expression of Akt1^Scer\UAS.cSa under the control of Scer\GAL4^GMR.PF results in a significant increase in the area of the ommatidia compared to control flies.

(Kramer et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

Akt^UAS.cSa, Scer\GAL4^GMR.PF has visible phenotype, non-suppressible by Snoo^EP2510, Scer\GAL4^GMR.PF

(Ramel et al., 2007)

Suppressor of

Statement

Reference

Akt^UAS.cSa, Scer\GAL4^GMR.PF is a suppressor of visible phenotype of Scer\GAL4^GMR.PF, foxo^GS9928

(Vereshchagina et al., 2008)

Akt^UAS.cSa, Scer\GAL4^GMR.PF is a suppressor of visible phenotype of Scer\GAL4^GMR.PF, foxo^GS9928, wdb^GS9548

(Vereshchagina et al., 2008)

Akt^UAS.cSa, Scer\GAL4^GMR.PF is a suppressor of visible phenotype of Scer\GAL4^GMR.PF, foxo^UAS.cKa

(Kramer et al., 2003)

NOT Suppressor of

Statement

Reference

Akt^UAS.cSa, Scer\GAL4^GMR.PF is a non-suppressor of visible phenotype of Mmus\Foxo1^AA.UAS, Scer\GAL4^GMR.PF

(Kramer et al., 2003)

Phenotype Manifest In

Suppressed by

Statement

Reference

Akt^UAS.cSa has eye phenotype, suppressible by egr^UAS.cMa/Scer\GAL4^GMR.PF

(Tsuda et al., 2010)

NOT suppressed by

Statement

Reference

Akt^UAS.cSa, Scer\GAL4^GMR.PF has eye phenotype, non-suppressible by Snoo^EP2510, Scer\GAL4^GMR.PF

(Ramel et al., 2007)

Enhancer of

Statement

Reference

Akt^UAS.cSa, Scer\GAL4^GMR.PF is an enhancer of eye phenotype of POSH^UAS.cSa, Scer\GAL4^GMR.PF, egr^UAS.cMa

(Tsuda et al., 2010)

POSH^UAS.cSa/Akt^UAS.cSa, Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Scer\GAL4^GMR.PF, egr^UAS.cMa

(Tsuda et al., 2010)

NOT Enhancer of

Statement

Reference

Akt^UAS.cSa, Scer\GAL4^GMR.PF is a non-enhancer of eye phenotype of Scer\GAL4^GMR.PF, egr^UAS.cMa

(Tsuda et al., 2010)

Suppressor of

Statement

Reference

Akt^UAS.cSa, Scer\GAL4^GMR.PF is a suppressor of eye phenotype of Scer\GAL4^GMR.PF, foxo^GS9928

(Vereshchagina et al., 2008)

Akt^UAS.cSa, Scer\GAL4^GMR.PF is a suppressor of eye phenotype of Scer\GAL4^GMR.PF, foxo^GS9928, wdb^GS9548

(Vereshchagina et al., 2008)

Scer\GAL4^GMR.PF/Akt^UAS.cSa is a suppressor of ommatidium phenotype of Scer\GAL4^GMR.PF, acs^GS10666, foxo^GS9928

(Goberdhan et al., 2005)

Akt^UAS.cSa, Scer\GAL4^GMR.PF is a suppressor of ommatidium phenotype of Scer\GAL4^GMR.PF, foxo^GS9928, path^GS13857

(Goberdhan et al., 2005)

Akt^UAS.cSa, Scer\GAL4^GMR.PF is a suppressor of ommatidium phenotype of Scer\GAL4^GMR.PF, foxo^GS9928

(Goberdhan et al., 2005)

Akt^UAS.cSa, Scer\GAL4^GMR.PF is a suppressor of ommatidium phenotype of Pi3K92E^{D954A.UAS.Tag:MYC}, Scer\GAL4^GMR.PF, foxo^GS9928

(Goberdhan et al., 2005)

Akt^UAS.cSa, Scer\GAL4^GMR.PF is a suppressor | partially of interommatidial bristle phenotype of Scer\GAL4^GMR.PF, foxo^UAS.cKa

(Kramer et al., 2003)

Akt^UAS.cSa, Scer\GAL4^GMR.PF is a suppressor of ommatidium phenotype of Scer\GAL4^GMR.PF, foxo^UAS.cKa

(Kramer et al., 2003)

NOT Suppressor of

Statement

Reference

Akt^UAS.cSa, Scer\GAL4^GMR.PF is a non-suppressor of interommatidial bristle phenotype of Mmus\Foxo1^AA.UAS, Scer\GAL4^GMR.PF

(Kramer et al., 2003)

Akt^UAS.cSa, Scer\GAL4^GMR.PF is a non-suppressor of ommatidium phenotype of Mmus\Foxo1^AA.UAS, Scer\GAL4^GMR.PF

(Kramer et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

The eye size increase observed in POSH^Scer\UAS.cSa egr^Scer\UAS.cMa (Scer\GAL4^GMR.PF overexpression mutants is enhanced upon co-expression of Akt1^Scer\UAS.cSa.

Overexpression of Akt1^Scer\UAS.cSa with egr^Scer\UAS.cMa in the developing eye (all under the control of Scer\GAL4^GMR.PF) does not increase eye size.

(Tsuda et al., 2010)

Expression of Akt1^Scer\UAS.cSa in animals expressing foxo^GS9928 under the control of Scer\GAL4^GMR.PF completely suppresses the eye phenotype.

(Vereshchagina et al., 2008)

Co-expression of Snoo^EP2510 does not suppress the eye overgrowth phenotype caused by expression of Akt1^Scer\UAS.cSa under the control of Scer\GAL4^GMR.PF.

(Ramel et al., 2007)

Co-expression of Akt1^Scer\UAS.cSa suppresses the Scer\GAL4^GMR.PF>foxo^GS9928 phenotype.

The ommatidial loss phenotype of foxo^GS9928 mutants is strongly enhanced by co-expressing Pi3K92E^{D954A.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^GMR.PF. This phenotype is completely suppressed by co-expression of Akt1^Scer\UAS.cSa.

Co-expression of Rheb^AV4 results in a strong enhancement of Scer\GAL4^GMR.PF>foxo^GS9928, leading to loss of virtually all ommatidia in females and pupal lethality in males with massive tissue degeneration in the head of late pupae. These phenotypes are partially suppressed by Akt1^Scer\UAS.cSa overexpression.

Co-expression of foxo^GS9928 with either CG1139^GS10666 or path^GS13857, driven by Scer\GAL4^GMR.PF, enhances the foxo^GS9928 reduced eye phenotype, an effect completely suppressed by Akt1^Scer\UAS.cSa co-expression.

(Goberdhan et al., 2005)

Co-expression of Akt1^Scer\UAS.cSa almost completely rescues the eye phenotype caused by expression of foxo^Scer\UAS.cKa under the control of Scer\GAL4^GMR.PF; the ommatidia and nearly all of the mechanosensory bristles are restored.

(Kramer et al., 2003)

Xenogenetic Interactions

Statement

Reference

Co-expression of Akt1^Scer\UAS.cSa does not rescue the eye phenotype caused by expression of Mmus\Foxo1^AA.Scer\UAS under the control of Scer\GAL4^GMR.PF. Co-expression of Akt1^Scer\UAS.cSa and Mmus\Foxo1^AA.Scer\UAS under the control of Scer\GAL4^GMR.PF results in ommatidia that are approximately the same size as in control flies.

(Kramer et al., 2003)

Complementation and Rescue Data

Rescues

Scer\GAL4^arm.PS/Akt^UAS.cSa rescues Akt¹

(Staveley et al., 1998)

Partially rescues

Scer\GAL4^arm.PU/Akt^UAS.cSa partially rescues Akt⁰⁴²²⁶

(Slade and Staveley, 2015)

Scer\GAL4^arm.PU/Akt^UAS.cSa partially rescues Akt⁸⁷

(Slade and Staveley, 2015)

Scer\GAL4^arm.PU/Akt^UAS.cSa partially rescues Akt⁵²

(Slade and Staveley, 2015)

Scer\GAL4^arm.PU/Akt^UAS.cSa partially rescues Akt⁵⁷

(Slade and Staveley, 2015)

Comments

Expression of Akt1^Scer\UAS.cSa under the control of Scer\GAL4^arm.PU partially rescues the reductions in ommatidial size and number seen in homozygous Akt1⁰⁴²²⁶ mutant flies.

Expression of Akt1^Scer\UAS.cSa under the control of Scer\GAL4^arm.PU partially rescues the reductions in ommatidial size and number seen in homozygous Akt1⁵² mutant flies.

Expression of Akt1^Scer\UAS.cSa under the control of Scer\GAL4^arm.PU partially rescues the reductions in ommatidial size and number seen in homozygous Akt1⁵⁷ mutant flies.

Expression of Akt1^Scer\UAS.cSa under the control of Scer\GAL4^arm.PU partially rescues the reductions in ommatidial size and number seen in homozygous Akt1⁸⁷ mutant flies.

(Slade and Staveley, 2015)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Akt1^Scer\UAS.cSa

Akt1^UAS.cSa

Akt^UAS.cSa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Staveley

(Staveley et al., 1998)

Secondary FlyBase IDs

References (9)

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