Akt1, dAkt, PKB, Dakt1, dPKB
Gene model reviewed during 5.55
Unconventional translation start (ACG) postulated; FBrf0079853.
Gene model reviewed during 5.54
Annotated transcripts do not represent all supported alternative splices within 5' UTR.
Low-frequency RNA-Seq exon junction(s) not annotated.
Interacts with trbl.
Binding of the PH domain to the phosphatidylinositol 3-kinase alpha (PI(3)K) results in its targeting to the plasma membrane.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Akt using the Feature Mapper tool.
The 4kb Akt1 transcripts are detected throughout embryogenesis and larval stages on northern blots. They are also detected in pupal stages and in adult females. Akt transcripts are uniformly distributed at a high level in embryos and are observed in nurse cells during oogenesis.
GBrowse - Visual display of RNA-Seq signalsView Dmel\Akt in GBrowse 2
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Removed the '1' suffix from 'Akt1' because: (i) there is only one Akt gene in D. melanogaster, (ii) the encoded protein is not more similar to mammalian AKT1 vs AKT2/AKT3 (% amino acid similarity/identity is actually higher for AKT2/3), (iii) usage in the literature favours 'Akt' compared to 'Akt1'.
dsRNA made from templates generated with primers directed against this gene results in a reduction in cell size.
dsRNA made from templates generated with primers directed against this gene used to treat S2 cells.
dsRNA made from templates generated with primers directed against this gene does not significantly inhibit S6k phosphorylation in S2 cells.
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a phenotype when assayed in S2R+ cells: cells become retracted (unspread but flat). Kc167 cells are unaffected.
Mutants exhibit ectopic apoptosis during embryogenesis as judged by induction of membrane blebbing, DNA fragmentation and macrophage infiltration. Apoptosis caused by loss of Akt1 function is rescued by caspase suppression.