FlyBase Allele Report: Dmel\Akt[T342D.S505D.GMR]

General Information

Symbol

Dmel\Akt^{T342D.S505D.GMR}

Species

D. melanogaster

Name

FlyBase ID

FBal0157363

Feature type

allele

Associated gene

Dmel\Akt

Associated Insertion(s)

Carried in Construct

P{GMR-Akt.T342D.S505D}

Key Links

Transgenic product class

missense_variant

(Parks, 2004.5.12)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Parks, 2004.5.12)

Mutagen

in vitro construct

(Parks, 2004.5.12)

Progenitor genotype

Carried in construct

P{GMR-Akt.T342D.S505D}

Cytology

Description

GMR regulatory sequences drive expression of a mutated form of Akt1 (carries the amino acid replacements T342D and S505D).

(Parks, 2004.5.12)

Allele components

Component

Use(s)

Regulatory region(s)

GMR

engineered transcription regulatory region

Encoded product / tool

Akt

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates C9orf72 frontotemporal dementia and/or amyotrophic lateral sclerosis

modeled by Zzzz\GGGGCC^36.UAS

(Atilano et al., 2021)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible

(Kaplan et al., 2008)

Phenotype Manifest In

eye

(Kaplan et al., 2008)

ommatidium

(Kaplan et al., 2008)

Detailed Description

Statement

Reference

Flies expressing Akt1^{T342D.S505D.GMR} show an increase in eye size, ommatidia size and ommatidia number compared to controls.

(Kaplan et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Akt^{T342D.S505D.GMR}, Scer\GAL4^GMR.PFa is a suppressor | partially of decreased size | adult stage phenotype of Scer\GAL4^GMR.PFa, Zzzz\GGGGCC^36.UAS

(Atilano et al., 2021)

Akt^{T342D.S505D.GMR}, Scer\GAL4^GMR.PFa is a suppressor | partially of visible | adult stage phenotype of Scer\GAL4^GMR.PFa, Zzzz\GGGGCC^36.UAS

(Atilano et al., 2021)

Akt^{T342D.S505D.GMR} is a suppressor of visible phenotype of Hsap\LRRK2^{G2019S.UAS.cLa.Tag:FLAG}, Scer\GAL4^GMR.PU, grim^GMR.PU

(Chuang et al., 2014)

Akt^{T342D.S505D.GMR}, Scer\GAL4^GMR.PU is a suppressor of visible phenotype of Hsap\LRRK2^{R1441C.UAS.Tag:FLAG}, Scer\GAL4^GMR.PU, grim^GMR.PU

(Chuang et al., 2014)

NOT Suppressor of

Statement

Reference

Akt^{T342D.S505D.GMR} is a non-suppressor of visible phenotype of Lst8¹

(Wang et al., 2012)

Phenotype Manifest In

Suppressor of

Statement

Reference

Akt^{T342D.S505D.GMR}, Scer\GAL4^GMR.PFa is a suppressor | partially of eye phenotype of Scer\GAL4^GMR.PFa, Zzzz\GGGGCC^36.UAS

(Atilano et al., 2021)

Akt^{T342D.S505D.GMR} is a suppressor of eye phenotype of Hsap\LRRK2^{G2019S.UAS.cLa.Tag:FLAG}, Scer\GAL4^GMR.PU, grim^GMR.PU

(Chuang et al., 2014)

Akt^{T342D.S505D.GMR} is a suppressor of ommatidium phenotype of Hsap\LRRK2^{G2019S.UAS.cLa.Tag:FLAG}, Scer\GAL4^GMR.PU, grim^GMR.PU

(Chuang et al., 2014)

Akt^{T342D.S505D.GMR}, Scer\GAL4^GMR.PU is a suppressor of eye phenotype of Hsap\LRRK2^{R1441C.UAS.Tag:FLAG}, Scer\GAL4^GMR.PU, grim^GMR.PU

(Chuang et al., 2014)

Akt^{T342D.S505D.GMR}, Scer\GAL4^GMR.PU is a suppressor of ommatidium phenotype of Hsap\LRRK2^{R1441C.UAS.Tag:FLAG}, Scer\GAL4^GMR.PU, grim^GMR.PU

(Chuang et al., 2014)

NOT Suppressor of

Statement

Reference

Akt^{T342D.S505D.GMR} is a non-suppressor of ommatidium phenotype of Lst8¹

(Wang et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Akt1^{T342D.S505D.GMR} does not increase the average ommatidium size in the eyes of Lst8¹ flies.

(Wang et al., 2012)

Xenogenetic Interactions

Statement

Reference

Expression of a constitutively active form of Akt1 (Akt1^{T342D.S505D.GMR}) significantly rescues the eye phenotype seen when grim^GMR.PU is co-expressed with Hsap\LRRK2^{G2019S.Scer\UAS.cLa.T:Zzzz\FLAG} (under the control of Scer\GAL4^GMR.PU).

Expression of a constitutively active form of Akt1 (Akt1^{T342D.S505D.GMR}) significantly rescues the eye phenotype seen when grim^GMR.PU is co-expressed with Hsap\LRRK2^{R1441C.Scer\UAS.T:Zzzz\FLAG} (under the control of Scer\GAL4^GMR.PU).

(Chuang et al., 2014)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

8193

P{GMR-Akt.T342D.S505D}1, y¹ w¹¹¹⁸

8194

y¹ w¹¹¹⁸; P{GMR-Akt.T342D.S505D}2

Notes on Origin

Discoverer

Comments

The encoded Akt1 protein variant is constitutively active.

(Parks, 2004.5.12)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Akt1^{T342D.S505D.GMR}

Akt^{T342D.S505D.GMR}

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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