FlyBase Allele Report: Dmel\Lst8[1]

General Information

Symbol

Dmel\Lst8¹

Species

D. melanogaster

Name

FlyBase ID

FBal0270246

Feature type

allele

Associated gene

Dmel\Lst8

Associated Insertion(s)

TI{TI}Lst8¹

Carried in Construct

Key Links

Allele class

amorphic allele - genetic evidence

Mutagen

ends-out gene targeting

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Wang et al., 2012)

Mutagen

ends-out gene targeting

(Wang et al., 2012)

Progenitor genotype

Associated Insertion(s)

TI{TI}Lst8¹

Cytology

Description

Approximately 1kb of the Lst8 gene region (including the transcription and translation start sites) has been deleted and replaced by a w[+] marker.

(Wang et al., 2012)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size | somatic clone

(Wang et al., 2012)

decreased body size | adult stage

(Wang et al., 2012)

decreased cell size | somatic clone

(Wang et al., 2012)

Phenotype Manifest In

embryonic/larval fat body | somatic clone

(Wang et al., 2012)

ommatidium

(Wang et al., 2012)

ommatidium | somatic clone

(Wang et al., 2012)

Detailed Description

Statement

Reference

Homozygous adults are smaller than normal and show an approximately 25% reduction in body weight compared to controls.

The number of ommatidia is not reduced in homozygous flies, but the average size of a single ommatidium is reduced by approximately 29% in the mutant flies.

Homozygous eyes in an otherwise heterozygous background are smaller than normal and the average size of a single ommatidium is reduced compared to controls.

Cells in homozygous clones in the larval fat body are slightly smaller than controls, regardless of nutrient status.

Flow cytometry analysis of homozygous wing disc cells indicates that cell cycle phasing is normal in these cells.

(Wang et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

Lst8¹ has visible phenotype, non-suppressible by Akt^{T342D.S505D.GMR}

(Wang et al., 2012)

NOT Suppressor of

Statement

Reference

Lst8¹ is a non-suppressor of visible phenotype of Rheb^UAS.cSa, Scer\GAL4^GMR.PU, mTor^UAS.cWa, raptor^UAS.cWa

(Wang et al., 2012)

Lst8¹ is a non-suppressor of visible phenotype of Rheb^UAS.cSa, Scer\GAL4^en.PU, mTor^UAS.cWa, raptor^UAS.cWa

(Wang et al., 2012)

Lst8¹ is a non-suppressor of increased cell size phenotype of Rheb^UAS.cSa, Scer\GAL4^GMR.PU

(Wang et al., 2012)

Lst8¹ is a non-suppressor of visible phenotype of Rheb^UAS.cSa, Scer\GAL4^GMR.PU

(Wang et al., 2012)

Lst8¹ is a non-suppressor of visible phenotype of Rheb^UAS.cSa, Scer\GAL4^en.PU

(Wang et al., 2012)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

Lst8¹ has ommatidium phenotype, non-suppressible by Akt^{T342D.S505D.GMR}

(Wang et al., 2012)

Lst8¹ has wing blade posterior compartment phenotype, non-suppressible by Scer\GAL4^en.PU/Rheb^UAS.cSa

(Wang et al., 2012)

Lst8¹ has wing blade posterior compartment phenotype, non-suppressible by Scer\GAL4^en.PU/raptor^UAS.cWa/mTor^UAS.cWa/Rheb^UAS.cSa

(Wang et al., 2012)

NOT Suppressor of

Statement

Reference

Lst8¹ is a non-suppressor of eye phenotype of Rheb^UAS.cSa, Scer\GAL4^GMR.PU, mTor^UAS.cWa, raptor^UAS.cWa

(Wang et al., 2012)

Lst8¹ is a non-suppressor of ommatidium phenotype of Rheb^UAS.cSa, Scer\GAL4^GMR.PU, mTor^UAS.cWa, raptor^UAS.cWa

(Wang et al., 2012)

Lst8¹ is a non-suppressor of eye phenotype of Rheb^UAS.cSa, Scer\GAL4^GMR.PU

(Wang et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Lst8¹ does not suppress the increased cell size seen in the eyes of flies expressing Rheb^Scer\UAS.cSa under the control of Scer\GAL4^GMR.PU.

Lst8¹ des not suppress the overgrowth of the posterior compartment of the wing relative to the anterior compartment which is seen in flies expressing Rheb^Scer\UAS.cSa under the control of Scer\GAL4^en.PU.

Flies co-expressing Tor^Scer\UAS.cWa, raptor^Scer\UAS.cWa and Rheb^Scer\UAS.cSa under the control of Scer\GAL4^GMR.PU completely lack photoreceptor cells in the eye. Evidence of cell death, such as accumulation of prominent vacuoles and highly condensed particles, is seen. This phenotype is still seen if the flies are also mutant for Lst8¹.

Flies co-expressing Tor^Scer\UAS.cWa, raptor^Scer\UAS.cWa and Rheb^Scer\UAS.cSa under the control of Scer\GAL4^GMR.PU (using the temperature sensitive Scer\GAL80^ts.αTub84B allele to limit expression to 4 hours immediately following pupa formation) show a dramatic increase in ommatidial size compared to controls. This phenotype is still seen if the flies are also are mutant for Lst8¹.

Flies co-expressing Tor^Scer\UAS.cWa, raptor^Scer\UAS.cWa and Rheb^Scer\UAS.cSa under the control of Scer\GAL4^en.PU (using the temperature sensitive Scer\GAL80^ts.αTub84B allele to limit expression to 4 hours immediately following pupa formation) show overgrowth of the posterior compartment of the wing relative to the anterior compartment. This phenotype is still seen if the flies are also mutant for Lst8¹.

Co-expression of Akt1^{T342D.S505D.GMR} does not increase the average ommatidium size in the eyes of Lst8¹ flies.

foxo²¹ does not rescue the reduced body weight of Lst8¹ flies.

(Wang et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Lst8¹ is rescued by Lst8^UAS.cWa/Scer\GAL4^da.G32

(Wang et al., 2012)

Lst8¹ is rescued by Lst8^UAS.cWa/Scer\GAL4^GMR.PU

(Wang et al., 2012)

Comments

Expression of Lst8^Scer\UAS.cWa under the control of Scer\GAL4^da.G32 fully rescues the growth defect of Lst8¹ flies.

Expression of Lst8^Scer\UAS.cWa under the control of Scer\GAL4^GMR.PU rescues the decreased ommatidial size seen in Lst8¹ flies.

(Wang et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

53724

TI{TI}Lst8¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Lst8¹

(Garcia et al., 2024)

lst8¹

(Wang et al., 2012)

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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