FlyBase Allele Report: Dmel\raptor[UAS.cWa]

General Information

Symbol

Dmel\raptor^UAS.cWa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Wang

FlyBase ID

FBal0270252

Feature type

allele

Associated gene

Dmel\raptor

Associated Insertion(s)

Carried in Construct

P{UAS-raptor.W}

Key Links

Transgenic product class

cDNA

(Wang et al., 2012)

wild_type

(Wang et al., 2012)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Wang et al., 2012)

wild_type

(Wang et al., 2012)

Mutagen

in vitro construct

(Wang et al., 2012)

Progenitor genotype

Carried in construct

P{UAS-raptor.W}

Cytology

Description

UAS regulatory sequences drive expression of raptor cDNA LD12356.

(Wang et al., 2012)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

raptor

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

Rheb^UAS.cSa, Scer\GAL4^GMR.PU, mTor^UAS.cWa, raptor^UAS.cWa has visible phenotype, non-suppressible by Lst8^UAS.cWa, Scer\GAL4^GMR.PU

(Wang et al., 2012)

Rheb^UAS.cSa, Scer\GAL4^GMR.PU, mTor^UAS.cWa, raptor^UAS.cWa has visible phenotype, non-suppressible by Lst8¹

(Wang et al., 2012)

Rheb^UAS.cSa, Scer\GAL4^en.PU, mTor^UAS.cWa, raptor^UAS.cWa has visible phenotype, non-suppressible by Lst8^UAS.cWa, Scer\GAL4^en.PU

(Wang et al., 2012)

Rheb^UAS.cSa, Scer\GAL4^en.PU, mTor^UAS.cWa, raptor^UAS.cWa has visible phenotype, non-suppressible by Lst8¹

(Wang et al., 2012)

Other

Statement

Reference

Rheb^UAS.cSa, Scer\GAL4^GMR.PU, mTor^UAS.cWa, raptor^UAS.cWa has visible phenotype

(Wang et al., 2012)

Rheb^UAS.cSa, Scer\GAL4^en.PU, mTor^UAS.cWa, raptor^UAS.cWa has visible phenotype

(Wang et al., 2012)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

Rheb^UAS.cSa, Scer\GAL4^GMR.PU, mTor^UAS.cWa, raptor^UAS.cWa has eye phenotype, non-suppressible by Lst8^UAS.cWa, Scer\GAL4^GMR.PU

(Wang et al., 2012)

Rheb^UAS.cSa, Scer\GAL4^GMR.PU, mTor^UAS.cWa, raptor^UAS.cWa has eye phenotype, non-suppressible by Lst8¹

(Wang et al., 2012)

Rheb^UAS.cSa, Scer\GAL4^GMR.PU, mTor^UAS.cWa, raptor^UAS.cWa has ommatidium phenotype, non-suppressible by Lst8^UAS.cWa, Scer\GAL4^GMR.PU

(Wang et al., 2012)

Rheb^UAS.cSa, Scer\GAL4^GMR.PU, mTor^UAS.cWa, raptor^UAS.cWa has ommatidium phenotype, non-suppressible by Lst8¹

(Wang et al., 2012)

NOT Suppressor of

Statement

Reference

raptor^UAS.cWa, mTor^UAS.cWa, Rheb^UAS.cSa, Scer\GAL4^en.PU is a non-suppressor of wing blade posterior compartment phenotype of Lst8^UAS.cWa, Scer\GAL4^en.PU

(Wang et al., 2012)

Scer\GAL4^en.PU, raptor^UAS.cWa, mTor^UAS.cWa, Rheb^UAS.cSa is a non-suppressor of wing blade posterior compartment phenotype of Lst8¹

(Wang et al., 2012)

Other

Statement

Reference

Rheb^UAS.cSa, Scer\GAL4^GMR.PU, mTor^UAS.cWa, raptor^UAS.cWa has eye phenotype

(Wang et al., 2012)

Rheb^UAS.cSa, Scer\GAL4^GMR.PU, mTor^UAS.cWa, raptor^UAS.cWa has ommatidium phenotype

(Wang et al., 2012)

Rheb^UAS.cSa, Scer\GAL4^en.PU, mTor^UAS.cWa, raptor^UAS.cWa has wing blade posterior compartment phenotype

(Wang et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Flies co-expressing Tor^Scer\UAS.cWa, raptor^Scer\UAS.cWa and Rheb^Scer\UAS.cSa under the control of Scer\GAL4^GMR.PU completely lack photoreceptor cells in the eye. Evidence of cell death, such as accumulation of prominent vacuoles and highly condensed particles, is seen. This phenotype is still seen if the flies are either also co-expressing Lst8^Scer\UAS.cWa or are mutant for Lst8¹.

Flies co-expressing Tor^Scer\UAS.cWa, raptor^Scer\UAS.cWa and Rheb^Scer\UAS.cSa under the control of Scer\GAL4^GMR.PU (using the temperature sensitive Scer\GAL80^ts.αTub84B allele to limit expression to 4 hours immediately following pupa formation) show a dramatic increase in ommatidial size compared to controls. This phenotype is still seen if the flies are either also co-expressing Lst8^Scer\UAS.cWa or are mutant for Lst8¹.

Flies co-expressing Tor^Scer\UAS.cWa, raptor^Scer\UAS.cWa and Rheb^Scer\UAS.cSa under the control of Scer\GAL4^en.PU (using the temperature sensitive Scer\GAL80^ts.αTub84B allele to limit expression to 4 hours immediately following pupa formation) show overgrowth of the posterior compartment of the wing relative to the anterior compartment. This phenotype is still seen if the flies are either also co-expressing Lst8^Scer\UAS.cWa or are mutant for Lst8¹.

(Wang et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

T:Ivir \HA1

(Wang et al., 2012)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

raptor^Scer\UAS.cWa

raptor^UAS.cWa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Wang

Secondary FlyBase IDs

References (1)

Report Sections

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