FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Lst8UAS.cWa
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General Information
Symbol
Dmel\Lst8UAS.cWa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Wang
FlyBase ID
FBal0270248
Feature type
allele
Associated gene
Dmel\Lst8
Associated Insertion(s)
Carried in Construct
P{UAS-Lst8.W}
Key Links
Transgenic product class
cDNA
(Wang et al., 2012)
wild_type
(Wang et al., 2012)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Wang et al., 2012)
wild_type
(Wang et al., 2012)
Mutagen
in vitro construct
(Wang et al., 2012)
Progenitor genotype
Carried in construct
P{UAS-Lst8.W}
Cytology
Description

UAS regulatory sequences drive expression of Lst8 cDNA LD23129.

(Wang et al., 2012)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Lst8
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      NOT Suppressor of
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Flies co-expressing TorScer\UAS.cWa, raptorScer\UAS.cWa and RhebScer\UAS.cSa under the control of Scer\GAL4GMR.PU completely lack photoreceptor cells in the eye. Evidence of cell death, such as accumulation of prominent vacuoles and highly condensed particles, is seen. This phenotype is still seen if the flies are also co-expressing Lst8Scer\UAS.cWa.

      Flies co-expressing TorScer\UAS.cWa, raptorScer\UAS.cWa and RhebScer\UAS.cSa under the control of Scer\GAL4GMR.PU (using the temperature sensitive Scer\GAL80ts.αTub84B allele to limit expression to 4 hours immediately following pupa formation) show a dramatic increase in ommatidial size compared to controls. This phenotype is still seen if the flies are also co-expressing Lst8Scer\UAS.cWa.

      Flies co-expressing TorScer\UAS.cWa, raptorScer\UAS.cWa and RhebScer\UAS.cSa under the control of Scer\GAL4en.PU (using the temperature sensitive Scer\GAL80ts.αTub84B allele to limit expression to 4 hours immediately following pupa formation) show overgrowth of the posterior compartment of the wing relative to the anterior compartment. This phenotype is still seen if the flies are also co-expressing Lst8Scer\UAS.cWa.

      (Wang et al., 2012)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      Lst8UAS.cWa/Scer\GAL4da.G32 rescues Lst81

      (Wang et al., 2012)

      Lst8UAS.cWa/Scer\GAL4GMR.PU rescues Lst81

      (Wang et al., 2012)
      Comments

      Expression of Lst8Scer\UAS.cWa under the control of Scer\GAL4da.G32 fully rescues the growth defect of Lst81 flies.

      Expression of Lst8Scer\UAS.cWa under the control of Scer\GAL4GMR.PU rescues the decreased ommatidial size seen in Lst81 flies.

      (Wang et al., 2012)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Lst8Scer\UAS.cWa
      Lst8UAS.cWa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Wang
      Secondary FlyBase IDs
        References (1)