FlyBase Allele Report: Dmel\Akt[GD1361]

Knockdown of Akt1 through the expression of Akt1^GD1361, under the control of Scer\GAL4^ppl.PP, leads to small cell size and an ectopic lipid storage phenotype in the salivary gland.

(Liu et al., 2014)

The dendrites of ddaC neurons of larvae expressing Akt1^GD1361 under the control of Scer\GAL4^ppk.PG show reduced regeneration (after dendrite severing at 48 hours after egg laying) compared to wild-type controls.

(Song et al., 2012)

Expression of Akt1^GD1361 using Scer\GAL4^nub-AC-62 results in abnormally small wings in which cell size and cell number are decreased.

(Resnik-Docampo and de Celis, 2011)

Compared with controls, the age-related dysplasia of the intestinal epithelium is strongly reduced in flies expressing Akt1^GD1361 under the control of Scer\GAL4^esg-NP5130 and Scer\GAL80^ts.αTub84B.

Expression of Akt1^GD1361 under the control of Scer\GAL4^esg-NP5130 and Scer\GAL80^ts.αTub84B results in significant lifespan shortening compared with controls.

Compared with controls, female flies expressing Akt1^GD1361 under the control of Scer\GAL4^bun-GSG5961 show a moderate, but significant, reduction in intestinal cell proliferation at old age. These flies are significantly longer lived when exposed to RU486 than isogenic siblings exposed to mock treatment.

(Biteau et al., 2010)

Depending on the insertion line used, expression under the control of Scer\GAL4^Mef2.PR can result in early larval lethality or undefined lethality.

Expression under the control of Scer\GAL4^Mef2.PR results in grossly normal larval body wall muscles.

Expression under the control of Scer\GAL4^Mef2.PR results in sarcomeres with irregular aggregates of Z-lines in the larval body wall muscles.

(Schnorrer et al., 2010)

Knocking down Akt1 expression via Scer\GAL4^ppk.PG-driven expression of Akt1^GD1361 in class IV neurons causes a significant reduction in dendrite growth and dendrite coverage of larval hemisegments.

(Parrish et al., 2009)

Expression of Akt1^GD1361 under the control of Scer\GAL4^Bx-MS1096 leads to crinkled wings.

(Dietzl et al., 2007)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

1361

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Akt^GD1361, Scer\GAL4^nub-AC-62 has abnormal size phenotype, enhanceable by hppy^UAS.cMa, Scer\GAL4^nub-AC-62

(Resnik-Docampo and de Celis, 2011)

Akt^GD1361, Scer\GAL4^nub-AC-62 has decreased cell number phenotype, enhanceable by hppy^UAS.cMa, Scer\GAL4^nub-AC-62

(Resnik-Docampo and de Celis, 2011)

Akt^GD1361, Scer\GAL4^nub-AC-62 has decreased cell size phenotype, enhanceable by hppy^UAS.cMa, Scer\GAL4^nub-AC-62

(Resnik-Docampo and de Celis, 2011)

NOT Enhanced by

Statement

Reference

Akt^GD1361, Scer\GAL4^elav-C155 has abnormal starvation stress response | adult stage phenotype, non-enhanceable by mir-137^CR

(Saedi et al., 2024)

Akt^GD1361, Scer\GAL4^elav-C155 has abnormal starvation stress response | adult stage phenotype, non-enhanceable by mir-137^KO

(Saedi et al., 2024)

Enhancer of

Statement

Reference

Akt^GD1361, Scer\GAL4^nub-AC-62 is an enhancer of abnormal size phenotype of Scer\GAL4^nub-AC-62, hppy^UAS.cMa

(Resnik-Docampo and de Celis, 2011)

Akt^GD1361, Scer\GAL4^nub-AC-62 is an enhancer of decreased cell number phenotype of Scer\GAL4^nub-AC-62, hppy^UAS.cMa

(Resnik-Docampo and de Celis, 2011)

Akt^GD1361, Scer\GAL4^nub-AC-62 is an enhancer of decreased cell size phenotype of Scer\GAL4^nub-AC-62, hppy^UAS.cMa

(Resnik-Docampo and de Celis, 2011)

NOT Enhancer of

Statement

Reference

Scer\GAL4^elav-C155/Akt^GD1361 is a non-enhancer of abnormal starvation stress response | adult stage | semidominant phenotype of mir-137^CR

(Saedi et al., 2024)

Scer\GAL4^elav-C155/Akt^GD1361 is a non-enhancer of abnormal starvation stress response | adult stage | semidominant phenotype of mir-137^KO

(Saedi et al., 2024)

Scer\GAL80^ts.αTub84B, Akt^GD1361, Scer\GAL4^da.PU is a non-enhancer of lethal - all die during pupal stage | heat sensitive phenotype of Scer\GAL4^da.PU, Scer\GAL80^ts.αTub84B, fzr^RNAi.UAS.WIZ

(Neuert et al., 2017)

Suppressor of

Statement

Reference

Akt^GD1361, Scer\GAL4^{FRT.Rnor\Cd2.Act5C}, Scer\GAL80^ts.αTub84B is a suppressor of neoplasia phenotype of Ras85D^V12.UAS, Scer\GAL4^{FRT.Rnor\Cd2.Act5C}, Scer\GAL80^ts.αTub84B, dlg1^GD4689

(Willecke et al., 2011)

Akt^GD1361, Scer\GAL4^Hml.Δ is a suppressor | partially of increased cell number | third instar larval stage phenotype of Hsap\RUNX1::Hsap\RUNX1T1^UAS.cSa, Scer\GAL4^Hml.Δ

(Sinenko et al., 2010)

Akt^GD1361, Scer\GAL4^repo.PU is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, Scer\GAL4^repo.PU, btl::Egfr^λ.UAS

(Read et al., 2009)

Akt^GD1361, Scer\GAL4^repo.PU is a suppressor of neoplasia | third instar larval stage phenotype of Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, Scer\GAL4^repo.PU, btl::Egfr^λ.UAS

(Read et al., 2009)

NOT Suppressor of

Statement

Reference

Scer\GAL80^ts.αTub84B, Akt^GD1361, Scer\GAL4^da.PU is a non-suppressor of lethal - all die during pupal stage | heat sensitive phenotype of Scer\GAL4^da.PU, Scer\GAL80^ts.αTub84B, fzr^RNAi.UAS.WIZ

(Neuert et al., 2017)

Phenotype Manifest In

Enhanced by

Statement

Reference

Akt^GD1361, Scer\GAL4^nub-AC-62 has wing phenotype, enhanceable by hppy^UAS.cMa, Scer\GAL4^nub-AC-62

(Resnik-Docampo and de Celis, 2011)

Enhancer of

Statement

Reference

Akt^GD1361, Scer\GAL4^nub-AC-62 is an enhancer of wing phenotype of Scer\GAL4^nub-AC-62, hppy^UAS.cMa

(Resnik-Docampo and de Celis, 2011)

Akt^GD1361, Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Crtc^UAS.cWa, Scer\GAL4^GMR.PF

(Wang et al., 2008)

Suppressor of

Statement

Reference

Akt^GD1361, Scer\GAL4^Ugt36A1.PK is a suppressor of lamellocyte | larval stage phenotype of ND-75^GD6309, Scer\GAL4^Ugt36A1.PK

(Sinenko et al., 2011)

Akt^GD1361, Scer\GAL4^{FRT.Rnor\Cd2.Act5C}, Scer\GAL80^ts.αTub84B is a suppressor of eye phenotype of Ras85D^V12.UAS, Scer\GAL4^{FRT.Rnor\Cd2.Act5C}, Scer\GAL80^ts.αTub84B, dlg1^GD4689

(Willecke et al., 2011)

Akt^GD1361, Scer\GAL4^Hml.Δ is a suppressor | partially of embryonic/larval hemocyte | third instar larval stage phenotype of Hsap\RUNX1::Hsap\RUNX1T1^UAS.cSa, Scer\GAL4^Hml.Δ

(Sinenko et al., 2010)

Akt^GD1361, Scer\GAL4^repo.PU is a suppressor of glial cell | increased number | third instar larval stage phenotype of Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, Scer\GAL4^repo.PU, btl::Egfr^λ.UAS

(Read et al., 2009)

Akt^GD1361, Scer\GAL4^repo.PU is a suppressor of larval brain | third instar larval stage phenotype of Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, Scer\GAL4^repo.PU, btl::Egfr^λ.UAS

(Read et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of hppy^Scer\UAS.cMa and Akt1^GD1361 using Scer\GAL4^nub-AC-62 results in a strong synergistic phenotype of reduced wing size and reduced cell size and number.

(Resnik-Docampo and de Celis, 2011)

Expression of Akt1^GD1361 suppresses the increase in circulating lamellocytes seen when ND75^GD6309 is expressed under the control of Scer\GAL4^Dot.PK.

(Sinenko et al., 2011)

Knockdown of Akt1 in Ras85D^V12.Scer\UAS-dlg1^GD4689 cells, through expression of Akt1^GD1361, causes a complete suppression of neoplastic growth and even causes a near-complete absence of mutant eye cells. Only small structures, which contain few cells, remain.

(Willecke et al., 2011)

The Scer\GAL4^ppk.PG-driven Akt1^GD1361-phenotype is epistatic to the dendrite overgrowth seen in Df(3L)ban^Δ1 mutants. In addition, reducing Akt1 expression by Scer\GAL4^ppk.PG-driven Akt1^GD1361 blocks the exuberant dendrite invasion activity of Df(3L)ban^Δ1 mutants.

(Parrish et al., 2009)

Expression of Akt1^GD1361 strongly suppresses the glial neoplasia seen in third instar larvae when btl::Egfr^{Scer\UAS.T:λ\cI-DD} and Pi3K92E^{Scer\UAS.T:Hsap\MYC,T:Hsap\CAAX} are co-expressed under the control of Scer\GAL4^repo.PU.

(Read et al., 2009)

Depletion of Akt1 through expression of Akt1^GD1361 in TORC^Scer\UAS.cWa-expressing developing eyes (both lines under the control of Scer\GAL4^GMR.PF) enhances the TORC-mediated rough eye phenotype.

(Wang et al., 2008)

Xenogenetic Interactions

Statement

Reference

Expression of Akt1^GD1361 strongly suppresses the hemocyte overproliferation seen in third instar larvae when Hsap\RUNX1::Hsap\RUNX1T1^Scer\UAS.cSa is expressed under the control of Scer\GAL4^Hml.Δ.

(Sinenko et al., 2010)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v2902

w¹¹¹⁸; P{GD1361}v2902

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

1361

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Akt1^GD1361

Akt^GD1361

Name Synonyms

Secondary FlyBase IDs

References (25)

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