FlyBase Allele Report: Dmel\dlg1[GD4689]

General Information

Symbol

Dmel\dlg1^GD4689

Species

D. melanogaster

Name

FlyBase ID

FBal0209360

Feature type

allele

Associated gene

Dmel\dlg1

Associated Insertion(s)

Carried in Construct

P{GD4689}

Also Known As

UAS-dlg^RNAi, dlg-IR, dlg RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD4689}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD4689

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of chromosome 3q29 microdeletion syndrome

CEA

(Yusuff et al., 2020)

CEA with Cbp20^KK109448

(Singh et al., 2020)

model of carcinoma

CEA with Ras85D^V12.UAS

(Santabárbara-Ruiz and Léopold, 2021)

CEA

(de Vreede et al., 2022)

model of cancer

CEA with Ras85D^V12.UAS

(Muzzopappa et al., 2017, Willecke et al., 2011)

CEA

(Yang et al., 2022, Parisi et al., 2014)

CEA with Ras85D^V12.cUa.UAS

(Yang et al., 2022)

model of developmental disorder of mental health

CEA

(Stessman et al., 2017)

model of brain cancer

CEA with Ras85D^V12.cUa.UAS

(Manzanero-Ortiz et al., 2021)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of carcinoma

is exacerbated by grnd^KK109939

(de Vreede et al., 2022)

exacerbates Alzheimer's disease

modeled by Hsap\MAPT^V337M.UAS

(Shulman et al., 2011)

model of cancer

is exacerbated by egr^KK103432

(Parisi et al., 2014)

is ameliorated by Xbp1^UAS.s.Tag:V5

(Yang et al., 2022)

is ameliorated by Ire1^UAS.Tag:V5

(Yang et al., 2022)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell polarity, with Scer\GAL4^insc-Mz1407

(Mauri et al., 2014)

abnormal flight, with Scer\GAL4^elav.PLu

(Dietzl et al., 2007)

abnormal locomotor behavior, with Scer\GAL4^elav.PLu

(Dietzl et al., 2007)

abnormal pain response | adult stage, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

increased cell death | larval stage, with Scer\GAL4^ptc-559.1

(Sharp et al., 2021)

increased size | larval stage, with Scer\GAL4^ptc-559.1

(Sharp et al., 2021)

lethal, with Scer\GAL4^Mef2.PR

(Dietzl et al., 2007)

lethal, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

lethal - all die during P-stage, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

neoplasia | larval stage, with Scer\GAL4^sd-SG29.1

(Parisi et al., 2014)

neoplasia | somatic clone | third instar larval stage, with Scer\GAL4^Dll-md23

(Rives-Quinto et al., 2017)

partially lethal - majority die, with Scer\GAL4^elav.PLu

(Dietzl et al., 2007)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

antennal disc | somatic clone | third instar larval stage, with Scer\GAL4^Dll-md23

(Rives-Quinto et al., 2017)

wing disc, with Scer\GAL4^sd-SG29.1

(Parisi et al., 2014)

wing disc | larval stage, with Scer\GAL4^ptc-559.1

(Sharp et al., 2021)

wing hinge primordium | larval stage, with Scer\GAL4^ptc-559.1

(Sharp et al., 2021)

Detailed Description

Statement

Reference

Expression of dlg1^GD4689 (driven by Scer\GAL4^Dll-md23) in MARCM clones in the antennal discs in third instar larvae leads to tumorigenic overgrowth, while in the type II neuroblast lineage clones it results in occasional appearance of supernumerary neuroblasts but to overgrowth.

(Rives-Quinto et al., 2017)

Expression of dlg1^GD4689 under the control of Scer\GAL4^e22c does not induce formation of syncytia in third instar larval epidermis.

(Wang et al., 2015)

Expression of dlg1^GD4689 in all neuroblasts under the control of Scer\GAL4^insc-Mz1407 does not cause an obvious change in neuroblast numbers in the larval brain, however it does produce defects in the basal segregation of mira.

(Mauri et al., 2014)

The expression of dlg1^GD4689 under the control of Scer\GAL4^sd-SG29.1 induces wing disc overgrowths.

(Parisi et al., 2014)

Expression of dlg1^GD4689 (together with UAS-Dicer2 to enhance RNAi efficiency) under the control of Scer\GAL4^GMR.PU has no effect on adult eye appearance.

(Shulman et al., 2011)

Expression of dlg1^GD4689 in a MARCM third instar imaginal disc clone results in small tumours.

(Willecke et al., 2011)

Adults expressing dlg1^GD4689 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) show significantly reduced avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^Mef2.PR results in early pupal lethality.

(Schnorrer et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in lethality before the pupal stage.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

4689

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Ras85D^V12.UAS, Scer\GAL4^ap-md544, dlg1^GD4689 has neoplasia | larval stage phenotype, enhanceable by stg^UAS.cNa, Scer\GAL4^ap-md544

(Muzzopappa et al., 2017)

Scer\GAL4^sd-SG29.1, dlg1^GD4689 has neoplasia | larval stage phenotype, enhanceable by Scer\GAL4^sd-SG29.1/egr^KK103432

(Parisi et al., 2014)

Ras85D^V12.UAS, Scer\GAL4^{FRT.Rnor\Cd2.Act5C}, dlg1^GD4689 has neoplasia phenotype, enhanceable by yki^UAS.cHa, Scer\GAL4^{FRT.Rnor\Cd2.Act5C}

(Willecke et al., 2011)

NOT Enhanced by

Statement

Reference

Scer\GAL4^ptc-559.1, dlg1^GD4689 has increased cell death | larval stage phenotype, non-enhanceable by E(bx)^HMS00065, Scer\GAL4^ptc-559.1

(Sharp et al., 2021)

Scer\GAL4^ptc-559.1, dlg1^GD4689 has increased cell death | larval stage phenotype, non-enhanceable by Iswi^HMS00628, Scer\GAL4^ptc-559.1

(Sharp et al., 2021)

Suppressed by

Statement

Reference

Scer\GAL4^ptc-559.1, dlg1^GD4689 has increased size | larval stage phenotype, suppressible by E(bx)^HMS00065, Scer\GAL4^ptc-559.1

(Sharp et al., 2021)

Scer\GAL4^ptc-559.1, dlg1^GD4689 has increased size | larval stage phenotype, suppressible by Iswi^HMS00628, Scer\GAL4^ptc-559.1

(Sharp et al., 2021)

Scer\GAL4^ptc-559.1, dlg1^GD4689 has increased size | larval stage phenotype, suppressible | partially by E(bx)^Nurf301-4/E(bx)[+]

(Sharp et al., 2021)

Ras85D^V12.UAS, Scer\GAL4^{Act5C.CoinFLP-FRT}, dlg1^GD4689 has neoplasia | somatic clone | third instar larval stage phenotype, suppressible | somatic clone by sd^JF02514, Scer\GAL4^{Act5C.CoinFLP-FRT}

(Katheder et al., 2017)

Ras85D^V12.UAS, Scer\GAL4^{Act5C.CoinFLP-FRT}, dlg1^GD4689 has neoplasia | somatic clone | third instar larval stage phenotype, suppressible | somatic clone by yki^HMS00041, Scer\GAL4^{Act5C.CoinFLP-FRT}

(Katheder et al., 2017)

Ras85D^V12.UAS, Scer\GAL4^{Act5C.CoinFLP-FRT}, dlg1^GD4689 has neoplasia | somatic clone | cell non-autonomous | third instar larval stage phenotype, suppressible | partially | somatic clone by Pi3K92E^{D954A.UAS.Tag:MYC}, Scer\GAL4^{Act5C.CoinFLP-FRT}

(Katheder et al., 2017)

Ras85D^V12.UAS, Scer\GAL4^{Act5C.CoinFLP-FRT}, dlg1^GD4689 has neoplasia | somatic clone | third instar larval stage phenotype, suppressible | somatic clone by slif^UY681, Scer\GAL4^{Act5C.CoinFLP-FRT}

(Katheder et al., 2017)

Ras85D^V12.UAS, Scer\GAL4^{Act5C.CoinFLP-FRT}, dlg1^GD4689 has abnormal size | somatic clone | third instar larval stage phenotype, suppressible | somatic clone by sd^JF02514, Scer\GAL4^{Act5C.CoinFLP-FRT}

(Katheder et al., 2017)

Ras85D^V12.UAS, Scer\GAL4^{Act5C.CoinFLP-FRT}, dlg1^GD4689 has abnormal size | somatic clone | third instar larval stage phenotype, suppressible | somatic clone by yki^HMS00041, Scer\GAL4^{Act5C.CoinFLP-FRT}

(Katheder et al., 2017)

Ras85D^V12.UAS, Scer\GAL4^{Act5C.CoinFLP-FRT}, dlg1^GD4689 has abnormal size | somatic clone | cell non-autonomous | third instar larval stage phenotype, suppressible | partially | somatic clone by Pi3K92E^{D954A.UAS.Tag:MYC}, Scer\GAL4^{Act5C.CoinFLP-FRT}

(Katheder et al., 2017)

Ras85D^V12.UAS, Scer\GAL4^{Act5C.CoinFLP-FRT}, dlg1^GD4689 has abnormal size | somatic clone | third instar larval stage phenotype, suppressible | somatic clone by slif^UY681, Scer\GAL4^{Act5C.CoinFLP-FRT}

(Katheder et al., 2017)

Ras85D^V12.UAS, Scer\GAL4^ap-md544, dlg1^GD4689 has neoplasia | larval stage phenotype, suppressible | partially by trbl^UAS.cMa, Scer\GAL4^ap-md544

(Muzzopappa et al., 2017)

Ras85D^V12.UAS, Scer\GAL4^Act5C.PP, dlg1^GD4689 has neoplasia phenotype, suppressible by grnd^KK109939, Scer\GAL4^Act5C.PP

(Andersen et al., 2015)

Ras85D^V12.UAS, Scer\GAL4^ey.PU, dlg1^GD4689 has neoplasia | somatic clone phenotype, suppressible | partially by ImpL2^GD6004, Scer\GAL4^ey.PU

(Figueroa-Clarevega and Bilder, 2015)

Ras85D^V12.UAS, Scer\GAL4^{FRT.Rnor\Cd2.Act5C}, dlg1^GD4689 has neoplasia | somatic clone phenotype, suppressible by Pi3K92E^GD11228, Scer\GAL4^{FRT.Rnor\Cd2.Act5C}

(Willecke et al., 2011)

Ras85D^V12.UAS, Scer\GAL4^{FRT.Rnor\Cd2.Act5C}, Scer\GAL80^ts.αTub84B, dlg1^GD4689 has neoplasia phenotype, suppressible by Pi3K92E^GD11228, Scer\GAL4^{FRT.Rnor\Cd2.Act5C}, Scer\GAL80^ts.αTub84B

(Willecke et al., 2011)

Ras85D^V12.UAS, Scer\GAL4^{FRT.Rnor\Cd2.Act5C}, Scer\GAL80^ts.αTub84B, dlg1^GD4689 has neoplasia phenotype, suppressible by Akt^GD1361, Scer\GAL4^{FRT.Rnor\Cd2.Act5C}, Scer\GAL80^ts.αTub84B

(Willecke et al., 2011)

Ras85D^V12.UAS, Scer\GAL4^{FRT.Rnor\Cd2.Act5C}, dlg1^GD4689 has neoplasia phenotype, suppressible by Pi3K92E^1C1

(Willecke et al., 2011)

Ras85D^V12.UAS, Scer\GAL4^{FRT.Rnor\Cd2.Act5C}, dlg1^GD4689 has neoplasia phenotype, suppressible by InR^GD104, Scer\GAL4^{FRT.Rnor\Cd2.Act5C}

(Willecke et al., 2011)

Ras85D^V12.UAS, Scer\GAL4^{FRT.Rnor\Cd2.Act5C}, dlg1^GD4689 has neoplasia phenotype, suppressible by S6k^GD6646, Scer\GAL4^{FRT.Rnor\Cd2.Act5C}

(Willecke et al., 2011)

Ras85D^V12.UAS, Scer\GAL4^{FRT.Rnor\Cd2.Act5C}, dlg1^GD4689 has neoplasia phenotype, suppressible by Rheb^NIG.1081R, Scer\GAL4^{FRT.Rnor\Cd2.Act5C}

(Willecke et al., 2011)

Ras85D^V12.UAS, Scer\GAL4^{FRT.Rnor\Cd2.Act5C}, dlg1^GD4689 has neoplasia phenotype, suppressible by mTor^NIG.5092R, Scer\GAL4^{FRT.Rnor\Cd2.Act5C}

(Willecke et al., 2011)

Ras85D^V12.UAS, Scer\GAL4^{FRT.Rnor\Cd2.Act5C}, dlg1^GD4689 has neoplasia phenotype, suppressible by Pten^KK109278, Scer\GAL4^{FRT.Rnor\Cd2.Act5C}

(Willecke et al., 2011)

Ras85D^V12.UAS, Scer\GAL4^en.PU, dlg1^GD4689 has neoplasia phenotype, suppressible by Pi3K92E^GD11228, Scer\GAL4^en.PU

(Willecke et al., 2011)

Ras85D^V12.UAS, Scer\GAL4^en.PU, dlg1^GD4689 has increased cell growth rate phenotype, suppressible by stg^hs.PE2/CycE^UAS.cRa, Scer\GAL4^en.PU

(Willecke et al., 2011)

NOT suppressed by

Statement

Reference

Scer\GAL4^ptc-559.1, dlg1^GD4689 has increased cell death | larval stage phenotype, non-suppressible by E(bx)^HMS00065, Scer\GAL4^ptc-559.1

(Sharp et al., 2021)

Scer\GAL4^ptc-559.1, dlg1^GD4689 has increased cell death | larval stage phenotype, non-suppressible by Iswi^HMS00628, Scer\GAL4^ptc-559.1

(Sharp et al., 2021)

Ras85D^V12.UAS, Scer\GAL4^ap-md544, dlg1^GD4689 has increased cell number | larval stage phenotype, non-suppressible by rpr^lexAop.cSa/Ecol\lexA^GMR15B03

(Muzzopappa et al., 2017)

Ras85D^V12.UAS, Scer\GAL4^hh.PU, dlg1^GD4689 has neoplasia | larval stage phenotype, non-suppressible by rpr^lexAop.cSa/Ecol\lexA^GMR15B03

(Muzzopappa et al., 2017)

Ras85D^V12.UAS, Scer\GAL4^hh.PU, dlg1^GD4689 has neoplasia | larval stage phenotype, non-suppressible by rpr^UAS.C/rpr^lexAop.cSa/Ecol\lexA^GMR15B03/Scer\GAL4^He.PZ

(Muzzopappa et al., 2017)

Ras85D^V12.UAS, Scer\GAL4^ap-md544, dlg1^GD4689 has neoplasia | larval stage phenotype, non-suppressible by egr^RNAi.UAS, Scer\GAL4^ap-md544

(Muzzopappa et al., 2017)

Ras85D^V12.UAS, Scer\GAL4^hh.PU, dlg1^GD4689 has neoplasia | larval stage phenotype, non-suppressible by egr¹/egr¹

(Muzzopappa et al., 2017)

Ras85D^V12.UAS, Scer\GAL4^hh.PU, dlg1^GD4689 has neoplasia | larval stage phenotype, non-suppressible by grnd^KK109939, Scer\GAL4^hh.PU

(Muzzopappa et al., 2017)

Ras85D^V12.UAS, Scer\GAL4^hh.PU, dlg1^GD4689 has neoplasia | larval stage phenotype, non-suppressible by grnd^MI05292/grnd^MI05292

(Muzzopappa et al., 2017)

Ras85D^V12.UAS, Scer\GAL4^ap-md544, dlg1^GD4689 has neoplasia | larval stage phenotype, non-suppressible by rpr^lexAop.cSa/Ecol\lexA^GMR15B03

(Muzzopappa et al., 2017)

Ras85D^V12.UAS, Scer\GAL4^Act5C.PP, dlg1^GD4689 has neoplasia phenotype, non-suppressible by wgn^RNAi.UAS, Scer\GAL4^Act5C.PP

(Andersen et al., 2015)

Enhancer of

Statement

Reference

dlg1^GD4689, Scer\GAL4^GMR.PU is an enhancer of visible | adult stage phenotype of Hsap\MAPT^V337M.UAS, Scer\GAL4^GMR.PU

(Shulman et al., 2011)

dlg1^GD4689, Scer\GAL4^{FRT.Rnor\Cd2.Act5C} is an enhancer of neoplasia | somatic clone phenotype of Ras85D^V12.UAS, Scer\GAL4^{FRT.Rnor\Cd2.Act5C}

(Willecke et al., 2011)

NOT Enhancer of

Statement

Reference

dlg1^GD4689/Scer\GAL4^Dll-md23 is a non-enhancer of abnormal neuroanatomy | third instar larval stage | somatic clone phenotype of cno^R2

(Rives-Quinto et al., 2017)

dlg1^GD4689/Scer\GAL4^Dll-md23 is a non-enhancer of abnormal cell number | third instar larval stage | somatic clone phenotype of cno^R2

(Rives-Quinto et al., 2017)

dlg1^GD4689/Scer\GAL4^Dll-md23 is a non-enhancer of decreased cell size | third instar larval stage | somatic clone phenotype of cno^R2

(Rives-Quinto et al., 2017)

Other

Statement

Reference

Ras85D^V12.UAS, Scer\GAL4^{Act5C.CoinFLP-FRT}, dlg1^GD4689 has abnormal size | somatic clone | third instar larval stage phenotype

(Katheder et al., 2017)

Ras85D^V12.UAS, Scer\GAL4^{Act5C.CoinFLP-FRT}, dlg1^GD4689 has neoplasia | somatic clone | third instar larval stage phenotype

(Katheder et al., 2017)

Ras85D^V12.UAS, Scer\GAL4^ap-md544, dlg1^GD4689 has increased cell number | larval stage phenotype

(Muzzopappa et al., 2017)

Ras85D^V12.UAS, Scer\GAL4^ap-md544, dlg1^GD4689 has neoplasia | larval stage phenotype

(Muzzopappa et al., 2017)

Ras85D^V12.UAS, Scer\GAL4^hh.PU, dlg1^GD4689 has neoplasia | larval stage phenotype

(Muzzopappa et al., 2017)

Ras85D^V12.UAS, Scer\GAL4^hh.PU, dlg1^GD4689 has increased occurrence of cell division | larval stage phenotype

(Muzzopappa et al., 2017)

Ras85D^V12.UAS, Scer\GAL4^Act5C.PP, dlg1^GD4689 has neoplasia phenotype

(Andersen et al., 2015)

Ras85D^V12.UAS, Scer\GAL4^ey.PU, dlg1^GD4689 has neoplasia | somatic clone phenotype

(Figueroa-Clarevega and Bilder, 2015)

Scer\GAL4^insc-Mz1407, dlg1^GD4689, wake^GD12087 has increased occurrence of cell division phenotype

(Mauri et al., 2014)

Ras85D^V12.UAS, Scer\GAL4^{FRT.Rnor\Cd2.Act5C}, Scer\GAL80^ts.αTub84B, dlg1^GD4689 has neoplasia phenotype

(Willecke et al., 2011)

Ras85D^V12.UAS, Scer\GAL4^en.PU, dlg1^GD4689 has neoplasia phenotype

(Willecke et al., 2011)

Phenotype Manifest In

Enhanced by

Statement

Reference

Ras85D^V12.UAS, Scer\GAL4^ap-md544, dlg1^GD4689 has wing disc phenotype, enhanceable by stg^UAS.cNa, Scer\GAL4^ap-md544

(Muzzopappa et al., 2017)

Scer\GAL4^Dll-md23, dlg1^GD4689 has antennal disc | third instar larval stage | somatic clone phenotype, enhanceable | somatic clone by cno^R2/cno^R2

(Rives-Quinto et al., 2017)

Scer\GAL4^sd-SG29.1, dlg1^GD4689 has wing disc phenotype, enhanceable by Scer\GAL4^sd-SG29.1/egr^KK103432

(Parisi et al., 2014)

NOT Enhanced by

Statement

Reference

Scer\GAL4^ptc-559.1, dlg1^GD4689 has wing disc | larval stage phenotype, non-enhanceable by E(bx)^HMS00065, Scer\GAL4^ptc-559.1

(Sharp et al., 2021)

Scer\GAL4^ptc-559.1, dlg1^GD4689 has wing disc | larval stage phenotype, non-enhanceable by Iswi^HMS00628, Scer\GAL4^ptc-559.1

(Sharp et al., 2021)

Suppressed by

Statement

Reference

Scer\GAL4^ptc-559.1, dlg1^GD4689 has wing hinge primordium | larval stage phenotype, suppressible by E(bx)^HMS00065, Scer\GAL4^ptc-559.1

(Sharp et al., 2021)

Scer\GAL4^ptc-559.1, dlg1^GD4689 has wing hinge primordium | larval stage phenotype, suppressible by Iswi^HMS00628, Scer\GAL4^ptc-559.1

(Sharp et al., 2021)

Scer\GAL4^ptc-559.1, dlg1^GD4689 has wing disc | larval stage phenotype, suppressible | partially by E(bx)^Nurf301-4/E(bx)[+]

(Sharp et al., 2021)

Ras85D^V12.UAS, Scer\GAL4^{Act5C.CoinFLP-FRT}, dlg1^GD4689 has autophagosome | somatic clone | cell non-autonomous | third instar larval stage phenotype, suppressible | cell non-autonomous | somatic clone by sd^JF02514, Scer\GAL4^{Act5C.CoinFLP-FRT}

(Katheder et al., 2017)

Ras85D^V12.UAS, Scer\GAL4^{Act5C.CoinFLP-FRT}, dlg1^GD4689 has eye-antennal disc | somatic clone | cell non-autonomous | third instar larval stage phenotype, suppressible | cell non-autonomous | somatic clone by sd^JF02514, Scer\GAL4^{Act5C.CoinFLP-FRT}

(Katheder et al., 2017)

Ras85D^V12.UAS, Scer\GAL4^{Act5C.CoinFLP-FRT}, dlg1^GD4689 has eye-antennal disc | somatic clone | third instar larval stage phenotype, suppressible | somatic clone by slif^UY681, Scer\GAL4^{Act5C.CoinFLP-FRT}

(Katheder et al., 2017)

Ras85D^V12.UAS, Scer\GAL4^ap-md544, dlg1^GD4689 has wing disc phenotype, suppressible | partially by trbl^UAS.cMa, Scer\GAL4^ap-md544

(Muzzopappa et al., 2017)

Ras85D^V12.UAS, Scer\GAL4^{FRT.Rnor\Cd2.Act5C}, Scer\GAL80^ts.αTub84B, dlg1^GD4689 has eye phenotype, suppressible by Akt^GD1361, Scer\GAL4^{FRT.Rnor\Cd2.Act5C}, Scer\GAL80^ts.αTub84B

(Willecke et al., 2011)

Ras85D^V12.UAS, Scer\GAL4^{FRT.Rnor\Cd2.Act5C}, Scer\GAL80^ts.αTub84B, dlg1^GD4689 has eye phenotype, suppressible by Pi3K92E^GD11228, Scer\GAL4^{FRT.Rnor\Cd2.Act5C}, Scer\GAL80^ts.αTub84B

(Willecke et al., 2011)

Ras85D^V12.UAS, Scer\GAL4^en.PU, dlg1^GD4689 has wing disc phenotype, suppressible by Pi3K92E^GD11228, Scer\GAL4^en.PU

(Willecke et al., 2011)

Ras85D^V12.UAS, Scer\GAL4^en.PU, dlg1^GD4689 has wing disc phenotype, suppressible by stg^hs.PE2/CycE^UAS.cRa, Scer\GAL4^en.PU

(Willecke et al., 2011)

NOT suppressed by

Statement

Reference

Scer\GAL4^ptc-559.1, dlg1^GD4689 has wing disc | larval stage phenotype, non-suppressible by Iswi^HMS00628, Scer\GAL4^ptc-559.1

(Sharp et al., 2021)

Scer\GAL4^ptc-559.1, dlg1^GD4689 has wing disc | larval stage phenotype, non-suppressible by E(bx)^HMS00065, Scer\GAL4^ptc-559.1

(Sharp et al., 2021)

Ras85D^V12.UAS, Scer\GAL4^{Act5C.CoinFLP-FRT}, dlg1^GD4689 has autophagosome | somatic clone | cell non-autonomous | third instar larval stage phenotype, non-suppressible | cell non-autonomous | somatic clone by ImpL2^RNAi.UAS.cUa, Scer\GAL4^{Act5C.CoinFLP-FRT}

(Katheder et al., 2017)

Ras85D^V12.UAS, Scer\GAL4^{Act5C.CoinFLP-FRT}, dlg1^GD4689 has eye-antennal disc | somatic clone | cell non-autonomous | third instar larval stage phenotype, non-suppressible | cell non-autonomous | somatic clone by ImpL2^RNAi.UAS.cUa, Scer\GAL4^{Act5C.CoinFLP-FRT}

(Katheder et al., 2017)

Ras85D^V12.UAS, Scer\GAL4^ap-md544, dlg1^GD4689 has wing disc phenotype, non-suppressible by egr^RNAi.UAS, Scer\GAL4^ap-md544

(Muzzopappa et al., 2017)

Ras85D^V12.UAS, Scer\GAL4^hh.PU, dlg1^GD4689 has wing disc phenotype, non-suppressible by egr¹/egr¹

(Muzzopappa et al., 2017)

Ras85D^V12.UAS, Scer\GAL4^hh.PU, dlg1^GD4689 has wing disc phenotype, non-suppressible by grnd^KK109939, Scer\GAL4^hh.PU

(Muzzopappa et al., 2017)

Ras85D^V12.UAS, Scer\GAL4^hh.PU, dlg1^GD4689 has wing disc phenotype, non-suppressible by grnd^MI05292/grnd^MI05292

(Muzzopappa et al., 2017)

Ras85D^V12.UAS, Scer\GAL4^ap-md544, dlg1^GD4689 has wing disc phenotype, non-suppressible by rpr^lexAop.cSa/Ecol\lexA^GMR15B03

(Muzzopappa et al., 2017)

Ras85D^V12.UAS, Scer\GAL4^ap-md544, dlg1^GD4689 has myoblast | increased number | larval stage phenotype, non-suppressible by rpr^lexAop.cSa/Ecol\lexA^GMR15B03

(Muzzopappa et al., 2017)

Ras85D^V12.UAS, Scer\GAL4^hh.PU, dlg1^GD4689 has wing disc phenotype, non-suppressible by rpr^lexAop.cSa/Ecol\lexA^GMR15B03

(Muzzopappa et al., 2017)

Ras85D^V12.UAS, Scer\GAL4^hh.PU, dlg1^GD4689 has wing disc phenotype, non-suppressible by rpr^UAS.C/rpr^lexAop.cSa/Ecol\lexA^GMR15B03/Scer\GAL4^He.PZ

(Muzzopappa et al., 2017)

Enhancer of

Statement

Reference

dlg1^GD4689, Scer\GAL4^GMR.PU is an enhancer of eye | adult stage phenotype of Hsap\MAPT^V337M.UAS, Scer\GAL4^GMR.PU

(Shulman et al., 2011)

NOT Enhancer of

Statement

Reference

dlg1^GD4689/Scer\GAL4^Dll-md23 is a non-enhancer of type II neuroblast | third instar larval stage | somatic clone phenotype of cno^R2

(Rives-Quinto et al., 2017)

Other

Statement

Reference

Ras85D^V12.UAS, Scer\GAL4^{Act5C.CoinFLP-FRT}, dlg1^GD4689 has eye-antennal disc | somatic clone | cell non-autonomous | third instar larval stage phenotype

(Katheder et al., 2017)

Ras85D^V12.UAS, Scer\GAL4^{Act5C.CoinFLP-FRT}, dlg1^GD4689 has autophagosome | somatic clone | cell non-autonomous | third instar larval stage phenotype

(Katheder et al., 2017)

Ras85D^V12.UAS, Scer\GAL4^ap-md544, dlg1^GD4689 has embryonic/larval hemocyte | increased number | larval stage phenotype

(Muzzopappa et al., 2017)

Ras85D^V12.UAS, Scer\GAL4^ap-md544, dlg1^GD4689 has myoblast | increased number | larval stage phenotype

(Muzzopappa et al., 2017)

Ras85D^V12.UAS, Scer\GAL4^hh.PU, dlg1^GD4689 has wing disc phenotype

(Muzzopappa et al., 2017)

Ras85D^V12.UAS, Scer\GAL4^ap-md544, dlg1^GD4689 has wing disc phenotype

(Muzzopappa et al., 2017)

Scer\GAL4^insc-Mz1407, dlg1^GD4689, wake^GD12087 has neuroblast | increased number phenotype

(Mauri et al., 2014)

Ras85D^V12.UAS, Scer\GAL4^en.PU, dlg1^GD4689 has wing disc phenotype

(Willecke et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Third instar larval eye-antennal imaginal disc clones co-expressing dlg1^GD4689 and Ras85D^V12.Scer\UAS under the control of Scer\GAL4^{Act5C.CoinFLP-FRT}, in combination with Dicer-2 for efficient RNAi, form invasive tumors and induce autophagosomes in a cell non-autonomous manner in neighboring disc tissue (assessed by a Atg8a fluorescence reporter), as compared to controls; both the tumor formation and the cell non-autonomous autophagosome induction are suppressed by the additional clonal co-expression of either sd^JF02514 or yki^HMS00041; the volume of these tumors is also significantly reduced by the co-expression of either slif^UY681 or Pi3K92E^{D954A.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^{Act5C.CoinFLP-FRT}; the cell non-autonomous increase in autophagosomes is also suppressed by the clonal co-expression of bsk^{DN.Scer\UAS.cUa}, but not of ImpL2^{dsRNA.Scer\UAS.cUa} or Pi3K92E^{D954A.Scer\UAS.T:Hsap\MYC}.

(Katheder et al., 2017)

The co-expression of Ras85D^V12.UAS and dlg1^GD4689 under the control of Scer\GAL4^ap-md544 induces wing disc neoplasia which, when transplanted, can form solid tumors in the host; there is amplification of the resident myoblast population, as well as accumulation of circulating hemocytes on the wing disc. Myoblast ablation (by expressing rpr^lexAop.cSa under the control of Ecol\lexA^GMR15B03) does not suppress the tumor-like growth.

The co-expression of Ras85D^V12.UAS and dlg1^GD4689 under the control of Scer\GAL4^hh.PU induces wing disc tumor-like growth, with increased mitotic index.; myoblast ablation (by expressing rpr^lexAop.cSa under the control of Ecol\lexA^GMR15B03) does not suppress the tumor-like growth. Similar tumor induction occurs if expression is induced in wing disc transplants (expression induction is controlled by Gal80[ts] and temperature-shift); hemocyte ablation on the host (by expressing rpr^UAS.C under the control of Scer\GAL4^He.PZ) together with myoblast ablation on the implant (by expressing rpr^UAS.C under the control of Ecol\lexA^GMR15B03) does not suppress the tumor-like growth.

(Muzzopappa et al., 2017)

The tumor-like overgrowth of MARCM clones in larval antennal disc expressing dlg1^GD4689 under the control of Scer\GAL4^Dll-md23 is not significantly worsened by cno^R2 homozygosity but the cellular and tissue morphology and disorganization are exacerbated.

The decreased size of neuroblasts in the cno^R2 type II neuroblast lineage MARCM clones is not affected by expression of dlg1^GD4689 (driven by Scer\GAL4^Dll-md23) in the clones.

(Rives-Quinto et al., 2017)

Eye discs co-expressing dlg1^GD4689 and Ras85D^V12.Scer\UAS in clones show tumour-like overgrowth and metastasis.

(Andersen et al., 2015)

Transplanted tissue fragments of Ras85D^V12.Scer\UAS donors also expressing dlg1^GD4689 under the control of Scer\GAL4^ey.PU cause non-autonomous wasting.

Expression of ImpL2^GD6004 within the tumors of Scer\GAL4^ey.PU>Ras85D^V12.Scer\UAS,dlg1^GD4689 origin significantly ameliorates the peripheral tissue wasting phenotypes. Hosts bearing dlg1^GD4689, Ras85D^V12.Scer\UAS, ImpL2^GD6004 tumors show increased abdominal fat body mass. Muscle function assays further reveal improvements in both climbing ability and speed. There is a significant rescue of ovariole health, leading to restoration of egg production.

(Figueroa-Clarevega and Bilder, 2015)

Co-expression of wake^GD12087 and dlg1^GD4689 in all neuroblasts under the control of Scer\GAL4^insc-Mz1407 causes massive overproliferation of neuroblasts, accompanied by increased mitotic activity.

(Mauri et al., 2014)

Expression of Ras85D^V12.Scer\UAS and dlg1^GD4689 in MARCM third instar imaginal disc clones results in a neoplastic phenotype.

Co-expression of Pi3K92E^GD11228 suppresses tumour growth in dlg1^GD4689 MARCM third instar imaginal disc clones, although this suppression is weaker than that seen upon expression of Pi3K92E^GD11228 in Ras85D^V12.Scer\UAS-dlg1^GD4689 MARCM third instar imaginal disc clones.

Knockdown of either Pi3K92E or Akt1 in Ras85D^V12.Scer\UAS-dlg1^GD4689 cells, through expression of either Pi3K92E^GD11228 or Akt1^GD1361, causes a complete suppression of neoplastic growth and even causes a near-complete absence of mutant eye cells. Only small structures, which contain few cells, remain.

MARCM Ras85D^V12.Scer\UAS-dlg1^GD4689 expressing clones are dramatically smaller in a Pi3K92E^1C1 homozygous mutant than in a wild-type background, indicating that a loss of Pi3K92E signaling interferes with Ras85D^V12.Scer\UAS-dlg1^GD4689 tumour growth.

Depletion of InR, through expression of InR^GD104, slightly reduces tumour size in Ras85D^V12.Scer\UAS-dlg1^GD4689 MARCM clones.

Depletion of S6k, through expression of S6k^GD6646, slightly reduces tumour size in Ras85D^V12.Scer\UAS-dlg1^GD4689 MARCM clones.

Depletion of Rheb, through expression of Rheb^NIG.1081R, strongly reduces tumour size in Ras85D^V12.Scer\UAS-dlg1^GD4689 MARCM clones.

Depletion of Tor, through expression of Tor^NIG.5092R, strongly reduces tumour size in Ras85D^V12.Scer\UAS-dlg1^GD4689 MARCM clones.

Depletion of Pten, through expression of Pten^KK109278, increases tumour size in Ras85D^V12.Scer\UAS-dlg1^GD4689 MARCM clones.

Overexpression of yki^Scer\UAS.cHa in dlg1^GD4689 MARCM clones generates larger tumorigenic overgrowths than in either single mutant clone.

Expression of Ras85D^V12.Scer\UAS and dlg1^GD4689 in the posterior compartment of wing discs, under the control of Scer\GAL4^en.PU results in an enlarged posterior wing disc compartment. These wing discs exhibit high levels of cell proliferation.

Co-expression of Pi3K92E^GD11228 together with Ras85D^V12.Scer\UAS and dlg1^GD4689, under the control of Scer\GAL4^en.PU, strongly reduces the size of the Ras85D^V12.Scer\UAS-dlg1^GD4689 tumours in the posterior wing disc compartment. There is an almost complete absence of cellular proliferation in this compartment.

Wing disc compartments expressing Ras85D^V12.Scer\UAS, dlg1^GD4689, Pi3K92E^GD11228, together with CycE^Scer\UAS.cRa and stg^hs.PE2 show a rescue in compartment size and high levels of His3-positive cells.

(Willecke et al., 2011)

Xenogenetic Interactions

Statement

Reference

The moderate rough eye phenotype and reduced adult eye size characteristic for flies expressing Scer\GAL4^GMR.PU under the control of Hsap\MAPT^{V337M.Scer\UAS} is significantly worsened by co-expression of dlg1^GD4689 (together with UAS-Dicer2 to enhance RNAi efficiency), exacerbating the rough appearance.

(Shulman et al., 2011)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

VDRC

v41134

w¹¹¹⁸; P{GD4689}v41134

v41136

w¹¹¹⁸; P{GD4689}v41136/TM3

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

4689

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Dlg^RNAi#1

(Wang et al., 2015)

dlg1^GD4689

(Singh et al., 2020, Yusuff et al., 2020)

dlg1^RNAi

(Katheder et al., 2017)

dlg^RNAi

(Figueroa-Clarevega and Bilder, 2015)

Name Synonyms

Secondary FlyBase IDs

References (37)

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