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General Information
Symbol
Dmel\egrdsRNA.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0147162
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-eiger-RNAi, UAS-Eiger-IR, UAS-eigerRNAi
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt sequences drive expression of a head to head inverted repeat of the egr coding sequence.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of egrdsRNA.UAS under the control of Scer\GAL4sv.PJ leads to accumulation of lipid droplets in retinas of 1-day-old flies when compared to controls.

Knocking down egr through expression of egrdsRNA.Scer\UAS under the control of Scer\GAL4GMR.PF rescues the cell death found after H[[2]]O[[2]] application on larval eye discs.

Knockdown of egr in the fat body (through expression of egrdsRNA.Scer\UAS under the control of Scer\GAL4Lsp2.PH) significantly increases survival time during an Salmonella typhimurium infection. However, bacterial loads over this time remain unchanged, indicating that the extended survival is due to tolerance rather than resistance.

Knockdown of egr in hemocytes (through expression of egrdsRNA.Scer\UAS under the control of Scer\GAL4Hml.PG) does not affect fly survival when infected by Salmonella typhimurium.

Knockdown of egr in the fat body or hemocytes (through expression of egrdsRNA.Scer\UAS under the control of Scer\GAL4Lsp2.PH or Scer\GAL4Hml.PG) reduces melanization after Salmonella typhimurium infection.

Knockdown of egr in the fat body or hemocytes of Salmonella typhimurium infected flies(through expression of egrdsRNA.Scer\UAS under the control of Scer\GAL4Lsp2.PH or Scer\GAL4Hml.PG) reduces feeding rates compared to infected wild-type flies. This change in feeding rate may be responsible for some aspects of the egr phenotype, although it must be noted that even in the absence of infection, egr knockdown flies consume less food.

Expression of egrGS9830 under the control of Scer\GAL4GMR.PF results in ablation of the adult eye. Co-expression of egrIR.Scer\UAS under the control of Scer\GAL4GMR.PF suppresses this phenotype.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

Expression of egrdsRNA.Scer\UAS suppresses the overgrowth seen in eye-antennal disc clones expressing Ras85DV12.Scer\UAS under the control of Scer\GAL4Act.PU.

Expression of egrdsRNA.Scer\UAS under the control of Scer\GAL4egr-GAL4 partially suppresses the NMJ degeneration seen in Ank2f02001 mutant third instar larvae.

Expression of egrIR.Scer\UAS under the control of Scer\GAL4Act.PU only within scrib1 clones in the eye-antennal disc results in 30.7% pupal lethality, while expression of egrIR.Scer\UAS under the control of Scer\GAL4Act.PU both within scrib1 clones in the eye-imaginal disc and in the surrounding wild-type cells results in 51.3% pupal lethality.

The eye phenotype in Ras85DKP mutant flies is partially suppressed by ectopic expression of egrIR.Scer\UAS in the eye under the control of Scer\GAL4GMR.PF.

Xenogenetic Interactions
Statement
Reference

Knocking down egr in necrotic neurons in Scer\GAL4hs.2sev->Mmus\Gria1Lc.Scer\UAS/egrdsRNA.Scer\UAS flies rescues the eye size defect in Mmus\Gria1Lc.Scer\UAS-expressing flies without affecting the primary necrosis.

Co-expression of egrdsRNA.Scer\UAS does not suppress the wing defects caused by expression of HIV-1\VpuScer\UAS.cLa under the control of Scer\GAL4dpp.blk1.

Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
egrIR.Scer\UAS
egrdsRNA.Scer\UAS
egrdsRNA.UAS
Name Synonyms
Secondary FlyBase IDs
    References (14)