Open Close
General Information
Symbol
Dmel\ph-d505
Species
D. melanogaster
Name
FlyBase ID
FBal0013759
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
ph505, ph0
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Nucleotide change:

C2119879T

Amino acid change:

Q575term | ph-d-PA; Q397term | ph-d-PB

Reported amino acid change:

Q398term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. The mutant strain carries two nonsense mutations, one in Gln398 of ph-d and one ine Gln749 of ph-p.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Amino acid replacement: Q398term.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Disease
Evidence
References
model of  cancer
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Does not affect the position effect variegation (PEV) at the w locus caused by In(1)wm4 or PEV at the B locus caused by Dp(1;Y)BSCV.

Mutation changes the level of w expression in ph-plac+3 flies; eye colour is darker.

Ectopic acridine orange and nile blue staining in ventral epidermis.

Lack longitudinal tracts but longitudinal glia differentiate though fail to form an orientated scaffold.

Most of the ventral cuticle is missing in hemizygous embryos, and there are no denticle bands on the small region of cuticle present. CNS neurons are organised into small ganglion-like clusters of cells, with the axons bundled together at the centre of the cluster. ph-d505 neurons cultured in vitro extend normal axons. ph-d505, stg4 double mutants have a viable epidermis which is able to secrete cuticle. ph-d505 embryos derived from homozygous dl1 females have an intact tube of epidermis similar to that of embryos derived from dl1 females that are wild-type for ph-d. Cell death is seen at the anterior and posterior termini of the doubly mutant embryos. Virtually all epidermal cells die in ph-d505 embryos derived from Tl3.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

ph-d505 ph-p505 hemizygous males die as embryos, soon after germ band retraction has finished.

ph-d505 ph-p505 mutant clones in mosaic eye discs overproliferate. The effect of the clones is both autonomous and non-autonomous.

Imaginal discs composed predominantly of ph-d505 ph-p505 cells (generated using the FLP/cell-lethal method) are strongly overgrown. The mutant tissue shows defects in epithelial organisation and differentiation.

Irradiated ph-p505/+. ph-d505/+ animals show vein duplications in anterior territories and vesicles of tissue trapped between the wing surfaces in both anterior and posterior compartments of the wing. they also exhibit abnormal anterior wing margins, and bifurcated anterior wing compartments. Pattern bifurcation and duplication are also seen in the leg, associated with trapped vesicles within the leg.

25% of ph-d505/+; en54/+ double heterozygotes have a gap in the fourth wing vein at 25oC.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Partially rescued by

ph-d505 is partially rescued by ph-d+tSa

Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer

Associated with: ph-p505.

Induced with: ph-p505.

The "ph[505]" chromosome has mutations in both ph-p and ph-d.

Comments
Comments

The ph-d505 chromosome is null for both ph-d and ph-p.

Mosaic analysis indicates that the epidermal cell death caused by ph-d505 is autonomous. ph-d505 neurons cultured in vitro extend normal axons.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
References (18)