FlyBase Allele Report: Scer\GAL4[sv.PJ]

General Information

Symbol

Scer\GAL4^sv.PJ

Species

S. cerevisiae

Name

shaven promoter construct of Jiao

FlyBase ID

FBal0127274

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{spa-GAL4.J}

Also Known As

spa-Gal4, sparkling-gal4, spaGal4

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Jiao et al., 2001)

Progenitor genotype

Carried in construct

P{spa-GAL4.J}

Cytology

Description

A 7.1kb EcoRI genomic fragment of sv, extending from intron 2 into intron 4 and including the "spa" enhancer, is fused upstream of a minimal Hsp70 promoter controlling expression of a GAL4 driver. αTub84B trailer sequences are also present.

(Jiao et al., 2001)

Allele components

Component

Use(s)

Regulatory region(s)

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage -- pupal stage

cone cell

(Nagaraj and Banerjee, 2007)

third instar larval stage -- P-stage

cone cell

(Bloomington Drosophila Stock Center, 2017)

third instar larval stage -- pupal stage

cone cell

(Luebbering et al., 2013)

Additional Information

Statement

Reference

ScerGAL4^sv.PJ drives expression in cone cells.

(Bloomington Drosophila Stock Center, 2017)

Marker for

Reflects expression of

(Luebbering et al., 2013, Nagaraj and Banerjee, 2007)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell shape | pupal stage, with pan^ΔN.UAS

(Cordero and Cagan, 2010)

abnormal neuroanatomy | adult stage, with Tace^GD572

(Muliyil et al., 2020)

abnormal neuroanatomy | adult stage | cell autonomous, with Blimp-1^HMC04792

(Wang et al., 2022)

decreased cell number | adult stage, with Tace^GD572

(Muliyil et al., 2020)

decreased cell number | pupal stage, with pan^ΔN.UAS

(Cordero and Cagan, 2010)

decreased size | adult stage | cell autonomous, with Blimp-1^HMC04792

(Wang et al., 2022)

decreased size | P-stage, with Rho1^JF02809

(Blackie et al., 2021)

visible, with Poxn^UAS.cJa

(Jiao et al., 2001)

Phenotype Manifest In

adherens junction | P-stage, with Rho1^JF02809

(Blackie et al., 2021)

cone cell | P-stage, with Rho1^JF02809

(Blackie et al., 2021)

cone cell | pupal stage, with pan^ΔN.UAS

(Cordero and Cagan, 2010)

eye, with Poxn^UAS.cJa

(Jiao et al., 2001)

eye photoreceptor cell | adult stage | cell autonomous, with Blimp-1^HMC04792

(Wang et al., 2022)

eye photoreceptor cell | decreased number, with Tace^GD572

(Muliyil et al., 2020)

lipid droplet | adult stage | increased number, with egr^RNAi.UAS

(Muliyil et al., 2020)

lipid droplet | adult stage | increased number, with grnd^GD12580

(Muliyil et al., 2020)

lipid droplet | adult stage | increased number, with Tace^GD572

(Muliyil et al., 2020)

ommatidium, with Poxn^UAS.cJa

(Jiao et al., 2001)

pigment cell, with Delta^UAS.cUa

(Nagaraj and Banerjee, 2007)

retina, with egr^RNAi.UAS

(Muliyil et al., 2020)

retina, with grnd^GD12580

(Muliyil et al., 2020)

retina, with Tace^GD572

(Muliyil et al., 2020)

rhabdomere of eye photoreceptor cell | adult stage | cell autonomous, with Blimp-1^HMC04792

(Wang et al., 2022)

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Poxn^UAS.cJa, Scer\GAL4^sv.PJ has visible phenotype, enhanceable by sv^spa-pol

(Jiao et al., 2001)

Suppressor of

Statement

Reference

Scer\GAL4^sv.PJ/bmm^UAS.cGa is a suppressor of abnormal neuroanatomy | adult stage | progressive phenotype of Tace¹

(Muliyil et al., 2020)

Scer\GAL4^sv.PJ/bmm^UAS.cGa is a suppressor of decreased cell number | adult stage | progressive phenotype of Tace¹

(Muliyil et al., 2020)

Scer\GAL4^sv.PJ/Sod2^UAS.cMa is a suppressor of abnormal neuroanatomy | adult stage | progressive phenotype of Tace¹

(Muliyil et al., 2020)

Scer\GAL4^sv.PJ/Sod2^UAS.cMa is a suppressor of decreased cell number | adult stage | progressive phenotype of Tace¹

(Muliyil et al., 2020)

Phenotype Manifest In

Enhanced by

Statement

Reference

Poxn^UAS.cJa, Scer\GAL4^sv.PJ has eye phenotype, enhanceable by sv^spa-pol

(Jiao et al., 2001)

Poxn^UAS.cJa, Scer\GAL4^sv.PJ has ommatidium phenotype, enhanceable by sv^spa-pol

(Jiao et al., 2001)

Suppressor of

Statement

Reference

Scer\GAL4^sv.PJ/bmm^UAS.cGa is a suppressor of lipid droplet | increased number | adult stage phenotype of Tace¹

(Muliyil et al., 2020)

Scer\GAL4^sv.PJ/bmm^UAS.cGa is a suppressor of pigment cell | decreased number phenotype of Tace¹

(Muliyil et al., 2020)

Scer\GAL4^sv.PJ/Cat^UAS.cAa is a suppressor | partially of lipid droplet | increased number | adult stage phenotype of Tace¹

(Muliyil et al., 2020)

Scer\GAL4^sv.PJ/bmm^UAS.cGa is a suppressor of eye photoreceptor cell | decreased number phenotype of Tace¹

(Muliyil et al., 2020)

Scer\GAL4^sv.PJ/bmm^UAS.cGa is a suppressor of retina phenotype of Tace¹

(Muliyil et al., 2020)

Scer\GAL4^sv.PJ/Sod2^UAS.cMa is a suppressor of retina phenotype of Tace¹

(Muliyil et al., 2020)

Scer\GAL4^sv.PJ/Sod1^UAS.cUa is a suppressor | partially of retina phenotype of Tace¹

(Muliyil et al., 2020)

Scer\GAL4^sv.PJ/Sod2^UAS.cMa is a suppressor of lipid droplet | increased number | adult stage phenotype of Tace¹

(Muliyil et al., 2020)

Scer\GAL4^sv.PJ/Sod1^UAS.cUa is a suppressor | partially of lipid droplet | increased number | adult stage phenotype of Tace¹

(Muliyil et al., 2020)

Scer\GAL4^sv.PJ/Sod2^UAS.cMa is a suppressor of eye photoreceptor cell | decreased number phenotype of Tace¹

(Muliyil et al., 2020)

Scer\GAL4^sv.PJ/Cat^UAS.cAa is a suppressor | partially of retina phenotype of Tace¹

(Muliyil et al., 2020)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^sv.PJ/Tace^UAS.cMa rescues Tace¹

(Muliyil et al., 2020)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

26656

P{spa-GAL4.J}1, w^*

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Scer\GAL4^sv.PJ

Name Synonyms

shaven promoter construct of Jiao

(Jiao et al., 2001)

Secondary FlyBase IDs

References (15)

Report Sections