FlyBase Allele Report: HIV-1\Vpu[UAS.cLa]

General Information

Symbol

HIV-1\Vpu^UAS.cLa

Species

L. Human immunodeficiency virus type 1

Name

Saccharomyces cerevisiae UAS construct a of Leulier

FlyBase ID

FBal0240710

Feature type

allele

Associated gene

HIV-1\Vpu

Associated Insertion(s)

Carried in Construct

P{UAS-Vpu.L}

Also Known As

UAS-Vpu

Transgenic product class

wild_type

(Leulier et al., 2003)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Leulier et al., 2003)

Mutagen

in vitro construct

(Leulier et al., 2003)

Progenitor genotype

Carried in construct

P{UAS-Vpu.L}

Cytology

Description

UASt regulatory sequences drive expression of HIV-1\Vpu.

(Leulier et al., 2003)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

HIV-1\Vpu

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of human immunodeficiency virus infectious disease

CEA

(Marchal et al., 2012, Leulier et al., 2003)

model of fungal infectious disease

CEA

(Leulier et al., 2003)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of human immunodeficiency virus infectious disease

is ameliorated by BacA\p35^UAS.cHa

(Marchal et al., 2012)

is ameliorated by Diap1^UAS.cHa

(Marchal et al., 2012)

is ameliorated by Diap1^UY1835

(Marchal et al., 2012)

is ameliorated by Tak1^KK108611

(Marchal et al., 2012)

is ameliorated by Traf6^GD7146

(Marchal et al., 2012)

is ameliorated by bsk^NIG.5680R

(Marchal et al., 2012)

is ameliorated by hep^NIG.4353R

(Marchal et al., 2012)

is exacerbated by slmb^UAS.cGa

(Marchal et al., 2012)

is ameliorated by slpr^KK100726

(Marchal et al., 2012)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal immune response, with Scer\GAL4^yolk

(Leulier et al., 2003)

increased cell death, with Scer\GAL4^dpp.blk1

(Marchal et al., 2012)

visible, with Scer\GAL4^dpp.blk1

(Marchal et al., 2012)

visible, with Scer\GAL4^en-e16E

(Marchal et al., 2012)

visible, with Scer\GAL4^GMR.PFa

(Marchal et al., 2012)

Phenotype Manifest In

anterior crossvein, with Scer\GAL4^dpp.blk1

(Marchal et al., 2012)

eye, with Scer\GAL4^GMR.PFa

(Marchal et al., 2012)

first posterior wing cell, with Scer\GAL4^dpp.blk1

(Marchal et al., 2012)

submarginal wing cell, with Scer\GAL4^dpp.blk1

(Marchal et al., 2012)

wing, with Scer\GAL4^en-e16E

(Marchal et al., 2012)

wing disc, with Scer\GAL4^dpp.blk1

(Marchal et al., 2012)

wing disc posterior compartment, with Scer\GAL4^en-e16E

(Marchal et al., 2012)

wing pouch, with Scer\GAL4^dpp.blk1

(Marchal et al., 2012)

wing vein, with Scer\GAL4^en-e16E

(Marchal et al., 2012)

wing vein L3, with Scer\GAL4^dpp.blk1

(Marchal et al., 2012)

Detailed Description

Statement

Reference

Expression of HIV-1\Vpu^Scer\UAS.cLa under the control of Scer\GAL4^en-e16E results a reduction in size of the posterior compartment of the wing disc. In adults, there is a reduction in size of the entire wing, together with additional tissue loss and vein defects in the posterior compartment.

Expression of HIV-1\Vpu^Scer\UAS.cLa under the control of Scer\GAL4^dpp.blk1 at 26[o]C results in a slight reduction in the anterior part of the wing pouch of the wing disc. Increased cell death is seen in the wing disc. At the compartment boundary, some apoptotic cells expressing HIV-1\Vpu^Scer\UAS.cLa are misplaced posteriorly to the Scer\GAL4^dpp.blk1-expression domain and are extruded basally from the wing disc epithelium. In adults there is a loss of the intervein tissue between L3 and L4, loss of tissue (mainly in the anterior region) between veins L2 and L3, loss of part of L3 and loss of the proximal crossvein between veins L3 and L4.

Expression of HIV-1\Vpu^Scer\UAS.cLa under the control of Scer\GAL4^GMR.PFa results in a rough eye phenotype.

(Marchal et al., 2012)

Flies expressing HIV-1\Vpu^Scer\UAS.cLa under the control of Scer\GAL4^yolk show normal resistance to infection by the Gram negative bacterium Erwinia carotovora, but are more susceptible to natural infection by the entomopathogenic fungus Beauveria bassiana and direct injection of either Aspergillus fumigatus or Neurospora crassa spores than controls.

(Leulier et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

HIV-1\Vpu^UAS.cLa, Scer\GAL4^dpp.blk1 has visible phenotype, non-enhanceable by slmb^KK108569, Scer\GAL4^dpp.blk1

(Marchal et al., 2012)

Suppressed by

Statement

Reference

HIV-1\Vpu^UAS.cLa, Scer\GAL4^dpp.blk1 has visible phenotype, suppressible by Diap1^UY1835, Scer\GAL4^dpp.blk1

(Marchal et al., 2012)

HIV-1\Vpu^UAS.cLa, Scer\GAL4^dpp.blk1 has visible phenotype, suppressible | partially by Diap1^UAS.cHa, Scer\GAL4^dpp.blk1

(Marchal et al., 2012)

HIV-1\Vpu^UAS.cLa, Scer\GAL4^dpp.blk1 has increased cell death phenotype, suppressible by Df(3L)H99/+

(Marchal et al., 2012)

HIV-1\Vpu^UAS.cLa, Scer\GAL4^dpp.blk1 has visible phenotype, suppressible by Df(3L)H99/+

(Marchal et al., 2012)

HIV-1\Vpu^UAS.cLa, Scer\GAL4^dpp.blk1 has increased cell death phenotype, suppressible by Diap1^UY1835, Scer\GAL4^dpp.blk1

(Marchal et al., 2012)

HIV-1\Vpu^UAS.cLa, Scer\GAL4^dpp.blk1 has visible phenotype, suppressible by Dronc^KK104278, Scer\GAL4^dpp.blk1

(Marchal et al., 2012)

HIV-1\Vpu^UAS.cLa, Scer\GAL4^dpp.blk1 has visible phenotype, suppressible | partially by Dronc^RNAi.UAS.cLa, Scer\GAL4^dpp.blk1

(Marchal et al., 2012)

HIV-1\Vpu^UAS.cLa, Scer\GAL4^dpp.blk1 has visible phenotype, suppressible | partially by Dronc^I24/Nc[+]

(Marchal et al., 2012)

HIV-1\Vpu^UAS.cLa, Scer\GAL4^dpp.blk1 has increased cell death phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^dpp.blk1

(Marchal et al., 2012)

HIV-1\Vpu^UAS.cLa, Scer\GAL4^dpp.blk1 has visible phenotype, suppressible | partially by BacA\p35^UAS.cHa, Scer\GAL4^dpp.blk1

(Marchal et al., 2012)

HIV-1\Vpu^UAS.cLa, Scer\GAL4^dpp.blk1 has visible phenotype, suppressible | partially by hep^NIG.4353R, Scer\GAL4^dpp.blk1

(Marchal et al., 2012)

HIV-1\Vpu^UAS.cLa, Scer\GAL4^dpp.blk1 has visible phenotype, suppressible by bsk^NIG.5680R, Scer\GAL4^dpp.blk1

(Marchal et al., 2012)

HIV-1\Vpu^UAS.cLa, Scer\GAL4^dpp.blk1 has visible phenotype, suppressible | partially by bsk¹/bsk[+]

(Marchal et al., 2012)

HIV-1\Vpu^UAS.cLa, Scer\GAL4^dpp.blk1 has visible phenotype, suppressible by Tak1^KK108611, Scer\GAL4^dpp.blk1

(Marchal et al., 2012)

HIV-1\Vpu^UAS.cLa, Scer\GAL4^dpp.blk1 has visible phenotype, suppressible | partially by Traf6^GD7146, Scer\GAL4^dpp.blk1

(Marchal et al., 2012)

HIV-1\Vpu^UAS.cLa, Scer\GAL4^dpp.blk1 has visible phenotype, suppressible | partially by slpr^KK100726, Scer\GAL4^dpp.blk1

(Marchal et al., 2012)

HIV-1\Vpu^UAS.cLa, Scer\GAL4^dpp.blk1 has visible phenotype, suppressible | partially by Mkk4^GD13804, Scer\GAL4^dpp.blk1

(Marchal et al., 2012)

NOT suppressed by

Statement

Reference

HIV-1\Vpu^UAS.cLa, Scer\GAL4^dpp.blk1 has visible phenotype, non-suppressible by slmb^UAS.cGa, Scer\GAL4^dpp.blk1

(Marchal et al., 2012)

HIV-1\Vpu^UAS.cLa, Scer\GAL4^GMR.PFa has visible phenotype, non-suppressible by Diap1^UY1835, Scer\GAL4^GMR.PFa

(Marchal et al., 2012)

HIV-1\Vpu^UAS.cLa, Scer\GAL4^dpp.blk1 has visible phenotype, non-suppressible by Traf4^GD10100, Scer\GAL4^dpp.blk1

(Marchal et al., 2012)

HIV-1\Vpu^UAS.cLa, Scer\GAL4^dpp.blk1 has visible phenotype, non-suppressible by msn^KK108948, Scer\GAL4^dpp.blk1

(Marchal et al., 2012)

HIV-1\Vpu^UAS.cLa, Scer\GAL4^dpp.blk1 has visible phenotype, non-suppressible by egr^RNAi.UAS, Scer\GAL4^dpp.blk1

(Marchal et al., 2012)

HIV-1\Vpu^UAS.cLa, Scer\GAL4^dpp.blk1 has visible phenotype, non-suppressible by wgn^RNAi.UAS, Scer\GAL4^dpp.blk1

(Marchal et al., 2012)

HIV-1\Vpu^UAS.cLa, Scer\GAL4^dpp.blk1 has visible phenotype, non-suppressible by msn[+]/msn¹⁰²

(Marchal et al., 2012)

HIV-1\Vpu^UAS.cLa, Scer\GAL4^dpp.blk1 has visible phenotype, non-suppressible by msn[+]/msn⁰⁶⁹⁴⁶

(Marchal et al., 2012)

HIV-1\Vpu^UAS.cLa, Scer\GAL4^dpp.blk1 has visible phenotype, non-suppressible by Rac1^J11/Rac1[+]

(Marchal et al., 2012)

HIV-1\Vpu^UAS.cLa, Scer\GAL4^dpp.blk1 has visible phenotype, non-suppressible by Rac1^J11/Rac2^Δ/Rac2[+]/Rac1[+]

(Marchal et al., 2012)

Other

Statement

Reference

BacA\p35^UAS.cHa, HIV-1\Vpu^UAS.cLa, Scer\GAL4^dpp.blk1 has visible phenotype

(Marchal et al., 2012)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

HIV-1\Vpu^UAS.cLa, Scer\GAL4^dpp.blk1 has wing phenotype, non-enhanceable by slmb^KK108569, Scer\GAL4^dpp.blk1

(Marchal et al., 2012)

Suppressed by

Statement

Reference

HIV-1\Vpu^UAS.cLa, Scer\GAL4^dpp.blk1 has wing phenotype, suppressible by Diap1^UY1835, Scer\GAL4^dpp.blk1

(Marchal et al., 2012)

HIV-1\Vpu^UAS.cLa, Scer\GAL4^dpp.blk1 has wing phenotype, suppressible | partially by Diap1^UAS.cHa, Scer\GAL4^dpp.blk1

(Marchal et al., 2012)

HIV-1\Vpu^UAS.cLa, Scer\GAL4^dpp.blk1 has wing phenotype, suppressible by Df(3L)H99/+

(Marchal et al., 2012)

HIV-1\Vpu^UAS.cLa, Scer\GAL4^dpp.blk1 has wing disc phenotype, suppressible by Df(3L)H99/+

(Marchal et al., 2012)

HIV-1\Vpu^UAS.cLa, Scer\GAL4^dpp.blk1 has wing disc phenotype, suppressible by Diap1^UY1835, Scer\GAL4^dpp.blk1

(Marchal et al., 2012)

HIV-1\Vpu^UAS.cLa, Scer\GAL4^dpp.blk1 has wing phenotype, suppressible by Dronc^KK104278, Scer\GAL4^dpp.blk1

(Marchal et al., 2012)

HIV-1\Vpu^UAS.cLa, Scer\GAL4^dpp.blk1 has wing phenotype, suppressible | partially by Dronc^RNAi.UAS.cLa, Scer\GAL4^dpp.blk1

(Marchal et al., 2012)

HIV-1\Vpu^UAS.cLa, Scer\GAL4^dpp.blk1 has wing phenotype, suppressible | partially by Dronc^I24/Nc[+]

(Marchal et al., 2012)

HIV-1\Vpu^UAS.cLa, Scer\GAL4^dpp.blk1 has wing vein L3 phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^dpp.blk1

(Marchal et al., 2012)

HIV-1\Vpu^UAS.cLa, Scer\GAL4^dpp.blk1 has submarginal wing cell phenotype, suppressible | partially by BacA\p35^UAS.cHa, Scer\GAL4^dpp.blk1

(Marchal et al., 2012)

HIV-1\Vpu^UAS.cLa, Scer\GAL4^dpp.blk1 has wing phenotype, suppressible | partially by hep^NIG.4353R, Scer\GAL4^dpp.blk1

(Marchal et al., 2012)

HIV-1\Vpu^UAS.cLa, Scer\GAL4^dpp.blk1 has wing phenotype, suppressible by bsk^NIG.5680R, Scer\GAL4^dpp.blk1

(Marchal et al., 2012)

HIV-1\Vpu^UAS.cLa, Scer\GAL4^dpp.blk1 has wing phenotype, suppressible | partially by bsk¹/bsk[+]

(Marchal et al., 2012)

HIV-1\Vpu^UAS.cLa, Scer\GAL4^dpp.blk1 has wing phenotype, suppressible by Tak1^KK108611, Scer\GAL4^dpp.blk1

(Marchal et al., 2012)

HIV-1\Vpu^UAS.cLa, Scer\GAL4^dpp.blk1 has wing phenotype, suppressible | partially by Traf6^GD7146, Scer\GAL4^dpp.blk1

(Marchal et al., 2012)

HIV-1\Vpu^UAS.cLa, Scer\GAL4^dpp.blk1 has wing phenotype, suppressible | partially by slpr^KK100726, Scer\GAL4^dpp.blk1

(Marchal et al., 2012)

HIV-1\Vpu^UAS.cLa, Scer\GAL4^dpp.blk1 has wing phenotype, suppressible | partially by Mkk4^GD13804, Scer\GAL4^dpp.blk1

(Marchal et al., 2012)

NOT suppressed by

Statement

Reference

HIV-1\Vpu^UAS.cLa, Scer\GAL4^dpp.blk1 has wing phenotype, non-suppressible by slmb^UAS.cGa, Scer\GAL4^dpp.blk1

(Marchal et al., 2012)

HIV-1\Vpu^UAS.cLa, Scer\GAL4^GMR.PFa has eye phenotype, non-suppressible by Diap1^UY1835, Scer\GAL4^GMR.PFa

(Marchal et al., 2012)

HIV-1\Vpu^UAS.cLa, Scer\GAL4^dpp.blk1 has wing phenotype, non-suppressible by Traf4^GD10100, Scer\GAL4^dpp.blk1

(Marchal et al., 2012)

HIV-1\Vpu^UAS.cLa, Scer\GAL4^dpp.blk1 has wing phenotype, non-suppressible by msn^KK108948, Scer\GAL4^dpp.blk1

(Marchal et al., 2012)

HIV-1\Vpu^UAS.cLa, Scer\GAL4^dpp.blk1 has wing phenotype, non-suppressible by egr^RNAi.UAS, Scer\GAL4^dpp.blk1

(Marchal et al., 2012)

HIV-1\Vpu^UAS.cLa, Scer\GAL4^dpp.blk1 has wing phenotype, non-suppressible by wgn^RNAi.UAS, Scer\GAL4^dpp.blk1

(Marchal et al., 2012)

HIV-1\Vpu^UAS.cLa, Scer\GAL4^dpp.blk1 has wing phenotype, non-suppressible by msn[+]/msn¹⁰²

(Marchal et al., 2012)

HIV-1\Vpu^UAS.cLa, Scer\GAL4^dpp.blk1 has wing phenotype, non-suppressible by msn[+]/msn⁰⁶⁹⁴⁶

(Marchal et al., 2012)

HIV-1\Vpu^UAS.cLa, Scer\GAL4^dpp.blk1 has wing phenotype, non-suppressible by Rac1^J11/Rac1[+]

(Marchal et al., 2012)

HIV-1\Vpu^UAS.cLa, Scer\GAL4^dpp.blk1 has wing phenotype, non-suppressible by Rac1^J11/Rac2^Δ/Rac2[+]/Rac1[+]

(Marchal et al., 2012)

Other

Statement

Reference

BacA\p35^UAS.cHa, HIV-1\Vpu^UAS.cLa, Scer\GAL4^dpp.blk1 has wing phenotype

(Marchal et al., 2012)

BacA\p35^UAS.cHa, HIV-1\Vpu^UAS.cLa, Scer\GAL4^dpp.blk1 has first posterior wing cell phenotype

(Marchal et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Co-expression of slmb^KK108569 does not enhance the wing defects caused by expression of HIV-1\Vpu^Scer\UAS.cLa under the control of Scer\GAL4^dpp.blk1.

Co-expression of slmb^Scer\UAS.cGa does not suppress the wing defects caused by expression of HIV-1\Vpu^Scer\UAS.cLa under the control of Scer\GAL4^dpp.blk1.

Co-expression of th^UY1835 suppresses the wing defects caused by expression of HIV-1\Vpu^Scer\UAS.cLa under the control of Scer\GAL4^dpp.blk1 but does not suppress the eye defects caused by expression of HIV-1\Vpu^Scer\UAS.cLa under the control of Scer\GAL4^GMR.PFa. The increased cell death seen in wing discs of animals expressing HIV-1\Vpu^Scer\UAS.cLa under the control of Scer\GAL4^dpp.blk1 are also suppressed by th^UY1835.

Co-expression of th^Scer\UAS.cHa partially suppresses the wing defects caused by expression of HIV-1\Vpu^Scer\UAS.cLa under the control of Scer\GAL4^dpp.blk1.

The increased cell death seen in wing discs of animals expressing HIV-1\Vpu^Scer\UAS.cLa under the control of Scer\GAL4^dpp.blk1 is suppressed by Df(3L)H99/+. The adult wing defects are also suppressed.

The adult wing defects caused by expression of HIV-1\Vpu^Scer\UAS.cLa under the control of Scer\GAL4^dpp.blk1 are partially suppressed by co-expression of Nc^{dsRNA.Scer\UAS.cLa} or by the presence of Nc^I24/+ and are more strongly suppressed by co-expression of Nc^KK104278.

Co-expression of BacA\p35^Scer\UAS.cHa completely suppresses apoptosis in cells in the wing disc expressing HIV-1\Vpu^Scer\UAS.cLa under the control of Scer\GAL4^dpp.blk1. The adult wing appears broadly disorganised in these animals, although there is recovery of a full-length L3 vein and partial restoration of issue between veins L2 and L3. An expansion of the space between veins L3 and L4 is seen in the double mutant flies, patches of cells seem to be excluded from the wing epithelium and the overall size of the wing is reduced.

Co-expression of either hep^NIG.4353R, Traf6^GD7146, slpr^KK100726 or Mkk4^GD13804 partially suppresses the wing defects caused by expression of HIV-1\Vpu^Scer\UAS.cLa under the control of Scer\GAL4^dpp.blk1.

Co-expression of either Tak1^KK108611 or bsk^NIG.5680R suppresses the wing defects caused by expression of HIV-1\Vpu^Scer\UAS.cLa under the control of Scer\GAL4^dpp.blk1.

bsk¹/+ partially suppresses the wing defects caused by expression of HIV-1\Vpu^Scer\UAS.cLa under the control of Scer\GAL4^dpp.blk1.

Co-expression of either Traf4^GD10100, msn^KK108948, egr^{dsRNA.Scer\UAS} or wgn^{dsRNA.Scer\UAS} does not suppress the wing defects caused by expression of HIV-1\Vpu^Scer\UAS.cLa under the control of Scer\GAL4^dpp.blk1.

Heterozygosity for msn¹⁰², msn⁰⁶⁹⁴⁶ or Rac1^J11 does not suppress the wing defects caused by expression of HIV-1\Vpu^Scer\UAS.cLa under the control of Scer\GAL4^dpp.blk1.

The presence of Rac1^J11/+ and Rac2^Δ/+ in double heterozygous combination does not suppress the wing defects caused by expression of HIV-1\Vpu^Scer\UAS.cLa under the control of Scer\GAL4^dpp.blk1.

(Marchal et al., 2012)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

HIV-1\Vpu^Scer\UAS.cLa

HIV-1\Vpu^UAS.cLa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Leulier

Secondary FlyBase IDs

References (2)

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