A 3475 base pair fragment from the flanking non-coding or intronic region of norpA is fused upstream of a Drosophila core synthetic promoter (DSCP) followed by sequence encoding a lexA::p65 driver. The driver sequence has been optimized for Drosophila codon usage. An Hsp70 transcriptional terminator is present.
The co-expression of rodGD8768 and BacA\p35UAS.cHa under the control of either Scer\GAL4ap-md544 or Scer\GAL4hh.PU induces wing disc tumor-like growth. Myoblast ablation (by expressing rprlexAop.cSa under the control of Ecol\lexAGMR15B03) does not suppress the tumor-like growth.
The co-expression of Ras85DV12.UAS and dlg1GD4689 under the control of Scer\GAL4ap-md544 induces wing disc neoplasia. Myoblast ablation on the host (by expressing rprlexAop.cSa under the control of Ecol\lexAGMR15B03) does not suppress the tumor-like growth.