FlyBase Allele Report: Dmel\psq[KK111691]

General Information

Symbol

Dmel\psq^KK111691

Species

D. melanogaster

Name

FlyBase ID

FBal0237600

Feature type

allele

Associated gene

Dmel\psq

Associated Insertion(s)

Carried in Construct

P{KK111691}

Also Known As

UAS-psq^RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

(Keleman et al., 2009.8.5)

Progenitor genotype

Carried in construct

P{KK111691}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Keleman et al., 2009.8.5)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-KK111691

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of cancer

CEA with Egfr^UAS.cBa

(Herranz et al., 2014)

model of carcinoma

CEA with Egfr^UAS.cBa

(Boukhatmi et al., 2020, Fereres et al., 2019)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

No obvious overgrowth is seen in wing discs expressing psq^KK111691 under the control of Scer\GAL4^ap-md544.

(Herranz et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Egfr^UAS.cBa, Scer\GAL4^ap-md544, psq^KK111691 has neoplasia | larval stage phenotype, suppressible by Nrg^HMS01638, Scer\GAL4^ap-md544

(Fereres et al., 2019)

Egfr^UAS.cBa, Scer\GAL4^ap-md544, psq^KK111691 has increased cell number | larval stage phenotype, suppressible by Nrg^HMS01638, Scer\GAL4^ap-md544

(Fereres et al., 2019)

Egfr^UAS.cBa, Scer\GAL4^ap-md544, psq^KK111691 has neoplasia | larval stage phenotype, suppressible by caps^ID.UAS, Scer\GAL4^ap-md544

(Fereres et al., 2019)

Egfr^UAS.cBa, Scer\GAL4^ap-md544, psq^KK111691 has increased cell number | larval stage phenotype, suppressible by caps^ID.UAS, Scer\GAL4^ap-md544

(Fereres et al., 2019)

Egfr^UAS.cBa, Scer\GAL4^ap-md544, psq^KK111691 has neoplasia | larval stage phenotype, suppressible by SCAR^GD11380, Scer\GAL4^ap-md544

(Fereres et al., 2019)

Egfr^UAS.cBa, Scer\GAL4^ap-md544, psq^KK111691 has increased cell number | larval stage phenotype, suppressible by SCAR^GD11380, Scer\GAL4^ap-md544

(Fereres et al., 2019)

Egfr^UAS.cBa, Scer\GAL4^ap-md544, psq^KK111691 has neoplasia | larval stage phenotype, suppressible by dia^GL00408, Scer\GAL4^ap-md544

(Fereres et al., 2019)

Egfr^UAS.cBa, Scer\GAL4^ap-md544, psq^KK111691 has neoplasia | larval stage phenotype, suppressible by dia^HM05027, Scer\GAL4^ap-md544

(Fereres et al., 2019)

Egfr^UAS.cBa, Scer\GAL4^ap-md544, psq^KK111691 has increased cell number | larval stage phenotype, suppressible by dia^HM05027, Scer\GAL4^ap-md544

(Fereres et al., 2019)

Egfr^UAS.cBa, Scer\GAL4^ap-md544, psq^KK111691 has neoplasia | larval stage phenotype, suppressible by Ecol\lexA^GMR15B03/dia^RNAi.lexAop

(Fereres et al., 2019)

Egfr^UAS.cBa, Scer\GAL4^ap-md544, psq^KK111691 has increased cell number | larval stage phenotype, suppressible by Ecol\lexA^GMR15B03/dia^RNAi.lexAop

(Fereres et al., 2019)

Egfr^UAS.cBa, Scer\GAL4^ap-md544, psq^KK111691 has neoplasia | larval stage phenotype, suppressible by btl^DN.UAS, Scer\GAL4^ap-md544

(Fereres et al., 2019)

Egfr^UAS.cBa, Scer\GAL4^ap-md544, psq^KK111691 has increased cell number | larval stage phenotype, suppressible by btl^DN.UAS, Scer\GAL4^ap-md544

(Fereres et al., 2019)

Egfr^UAS.cBa, Scer\GAL4^ap-md544, psq^KK111691 has abnormal cell polarity | larval stage phenotype, suppressible by dia^HM05027, Scer\GAL4^ap-md544

(Fereres et al., 2019)

Egfr^UAS.cBa, Scer\GAL4^ap-md544, psq^KK111691 has abnormal cell migration | larval stage phenotype, suppressible by dia^HM05027, Scer\GAL4^ap-md544

(Fereres et al., 2019)

Egfr^UAS.cBa, Scer\GAL4^ap-md544, psq^KK111691 has increased mortality during development phenotype, suppressible by Nrg^HMS01638, Scer\GAL4^ap-md544

(Fereres et al., 2019)

Egfr^UAS.cBa, Scer\GAL4^ap-md544, psq^KK111691 has increased mortality during development phenotype, suppressible by caps^ID.UAS, Scer\GAL4^ap-md544

(Fereres et al., 2019)

Egfr^UAS.cBa, Scer\GAL4^ap-md544, psq^KK111691 has increased mortality during development phenotype, suppressible by SCAR^GD11380, Scer\GAL4^ap-md544

(Fereres et al., 2019)

Egfr^UAS.cBa, Scer\GAL4^ap-md544, psq^KK111691 has increased mortality during development phenotype, suppressible by dia^HM05027, Scer\GAL4^ap-md544

(Fereres et al., 2019)

Egfr^UAS.cBa, Scer\GAL4^ap-md544, psq^KK111691 has increased mortality during development phenotype, suppressible by btl^DN.UAS, Scer\GAL4^ap-md544

(Fereres et al., 2019)

Egfr^UAS.cBa, Scer\GAL4^ap-md544, psq^KK111691 has partially lethal phenotype, suppressible by Nrg^HMS01638, Scer\GAL4^ap-md544

(Fereres et al., 2019)

Egfr^UAS.cBa, Scer\GAL4^ap-md544, psq^KK111691 has partially lethal phenotype, suppressible by caps^ID.UAS, Scer\GAL4^ap-md544

(Fereres et al., 2019)

Egfr^UAS.cBa, Scer\GAL4^ap-md544, psq^KK111691 has partially lethal phenotype, suppressible by SCAR^GD11380, Scer\GAL4^ap-md544

(Fereres et al., 2019)

Egfr^UAS.cBa, Scer\GAL4^ap-md544, psq^KK111691 has partially lethal - majority live phenotype, suppressible by dia^HM05027, Scer\GAL4^ap-md544

(Fereres et al., 2019)

NOT suppressed by

Statement

Reference

Egfr^UAS.cBa, Scer\GAL4^ap-md544, psq^KK111691 has neoplasia | larval stage phenotype, non-suppressible by fj^HMS01310, Scer\GAL4^ap-md544

(Fereres et al., 2019)

Egfr^UAS.cBa, Scer\GAL4^ap-md544, psq^KK111691 has increased cell number | larval stage phenotype, non-suppressible by fj^HMS01310, Scer\GAL4^ap-md544

(Fereres et al., 2019)

Egfr^UAS.cBa, Scer\GAL4^ap-md544, dia^HM05027, psq^KK111691 has neoplasia | larval stage phenotype, non-suppressible by Nrg^HMS01638, Scer\GAL4^ap-md544

(Fereres et al., 2019)

Egfr^UAS.cBa, Scer\GAL4^ap-md544, psq^KK111691 has neoplasia | larval stage phenotype, non-suppressible by ds^HMS00759, Scer\GAL4^ap-md544

(Fereres et al., 2019)

Egfr^UAS.cBa, Scer\GAL4^ap-md544, psq^KK111691 has increased cell number | larval stage phenotype, non-suppressible by ds^HMS00759, Scer\GAL4^ap-md544

(Fereres et al., 2019)

Egfr^UAS.cBa, Scer\GAL4^ap-md544, psq^KK111691 has neoplasia | larval stage phenotype, non-suppressible by ft^HMS00932, Scer\GAL4^ap-md544

(Fereres et al., 2019)

Egfr^UAS.cBa, Scer\GAL4^ap-md544, psq^KK111691 has increased cell number | larval stage phenotype, non-suppressible by ft^HMS00932, Scer\GAL4^ap-md544

(Fereres et al., 2019)

Enhancer of

Statement

Reference

psq^KK111691, Scer\GAL4^ap-md544 is an enhancer of hyperplasia phenotype of Egfr^UAS.cBa, Scer\GAL4^ap-md544

(Herranz et al., 2014)

Other

Statement

Reference

Egfr^UAS.cBa, Scer\GAL4^ap-md544, dia^HM05027, psq^KK111691 has fertile phenotype

(Fereres et al., 2019)

Egfr^UAS.cBa, Scer\GAL4^ap-md544, psq^KK111691 has lethal - all die before end of larval stage phenotype

(Fereres et al., 2019)

Egfr^UAS.cBa, Scer\GAL4^ap-md544, psq^KK111691 has neoplasia | larval stage phenotype

(Fereres et al., 2019)

Egfr^UAS.cBa, Scer\GAL4^ap-md544, dia^HM05027, psq^KK111691 has visible | adult stage phenotype

(Fereres et al., 2019)

Egfr^UAS.cBa, Scer\GAL4^ap-md544, btl^DN.UAS, psq^KK111691 has lethal - all die before end of pupal stage phenotype

(Fereres et al., 2019)

Egfr^UAS.cBa, Scer\GAL4^ap-md544, psq^KK111691 has abnormal developmental rate | larval stage phenotype

(Fereres et al., 2019)

Egfr^UAS.cBa, Scer\GAL4^ap-md544, psq^KK111691 has abnormal cell polarity | larval stage phenotype

(Fereres et al., 2019)

Egfr^UAS.cBa, Scer\GAL4^ap-md544, psq^KK111691 has increased cell number | larval stage phenotype

(Fereres et al., 2019)

Egfr^UAS.cBa, Scer\GAL4^ap-md544, psq^KK111691 has neoplasia phenotype

(Herranz et al., 2014)

Phenotype Manifest In

Suppressed by

Statement

Reference

Egfr^UAS.cBa, Scer\GAL4^ap-md544, psq^KK111691 has wing disc phenotype, suppressible by Nrg^HMS01638, Scer\GAL4^ap-md544

(Fereres et al., 2019)

Egfr^UAS.cBa, Scer\GAL4^ap-md544, psq^KK111691 has wing disc phenotype, suppressible by caps^ID.UAS, Scer\GAL4^ap-md544

(Fereres et al., 2019)

Egfr^UAS.cBa, Scer\GAL4^ap-md544, psq^KK111691 has wing disc phenotype, suppressible by SCAR^GD11380, Scer\GAL4^ap-md544

(Fereres et al., 2019)

Egfr^UAS.cBa, Scer\GAL4^ap-md544, psq^KK111691 has wing disc phenotype, suppressible by dia^GL00408, Scer\GAL4^ap-md544

(Fereres et al., 2019)

Egfr^UAS.cBa, Scer\GAL4^ap-md544, psq^KK111691 has wing disc phenotype, suppressible by dia^HM05027, Scer\GAL4^ap-md544

(Fereres et al., 2019)

Egfr^UAS.cBa, Scer\GAL4^ap-md544, psq^KK111691 has wing disc phenotype, suppressible by Ecol\lexA^GMR15B03/dia^RNAi.lexAop

(Fereres et al., 2019)

Egfr^UAS.cBa, Scer\GAL4^ap-md544, psq^KK111691 has embryonic/larval tracheal section phenotype, suppressible by dia^HM05027, Scer\GAL4^ap-md544

(Fereres et al., 2019)

Egfr^UAS.cBa, Scer\GAL4^ap-md544, psq^KK111691 has wing disc phenotype, suppressible by btl^DN.UAS, Scer\GAL4^ap-md544

(Fereres et al., 2019)

NOT suppressed by

Statement

Reference

Egfr^UAS.cBa, Scer\GAL4^ap-md544, dia^HM05027, psq^KK111691 has wing disc phenotype, non-suppressible by Nrg^HMS01638, Scer\GAL4^ap-md544

(Fereres et al., 2019)

Egfr^UAS.cBa, Nrg^HMS01638, Scer\GAL4^ap-md544, psq^KK111691 has wing disc phenotype, non-suppressible by dia^HM05027, Scer\GAL4^ap-md544

(Fereres et al., 2019)

Egfr^UAS.cBa, Scer\GAL4^ap-md544, psq^KK111691 has wing disc phenotype, non-suppressible by ds^HMS00759, Scer\GAL4^ap-md544

(Fereres et al., 2019)

Egfr^UAS.cBa, Scer\GAL4^ap-md544, psq^KK111691 has wing disc phenotype, non-suppressible by ft^HMS00932, Scer\GAL4^ap-md544

(Fereres et al., 2019)

Egfr^UAS.cBa, Scer\GAL4^ap-md544, psq^KK111691 has wing disc phenotype, non-suppressible by fj^HMS01310, Scer\GAL4^ap-md544

(Fereres et al., 2019)

Other

Statement

Reference

Egfr^UAS.cBa, Scer\GAL4^ap-md544, dia^HM05027, psq^KK111691 has wing blade phenotype

(Fereres et al., 2019)

Egfr^UAS.cBa, Scer\GAL4^ap-md544, psq^KK111691 has wing disc phenotype

(Fereres et al., 2019)

Egfr^UAS.cBa, Scer\GAL4^ap-md544, psq^KK111691 has embryonic/larval tracheal section phenotype

(Fereres et al., 2019)

Additional Comments

Genetic Interactions

Statement

Reference

All individuals co-expressing psq^KK111691 and Egfr^UAS.cBa under the control of Scer\GAL4^ap-md544 during larval stages (using Gal80[ts] for the temporal regulation of expression) are developmentally delayed and die at the third instar stage; their wing discs form epithelial tumors that exhibit cell polarity defects (i.e. Dlg mis-localization), associated with higher numbers of myoblasts and a more extensive/branched trachea than normal across the disc. The tumors metastasize to distant tissues in the larva.

The tumor phenotype (size and disc distortion) and the increased myoblast numbers are partially suppressed by the additional co-expression of either Nrg^HMS01638, caps^ID.UAS, SCAR^GD11380, or dia^HM05027, but not of ds^HMS00759, fj^HMS01310 or ft^HMS00932; dia^HM05027 also partially suppresses the increased tracheation. There is no further tumor suppression by Nrg^HMS01638, dia^HM05027 combined.

Co-expression of dia^dsRNA.lexAop under the control of Ecol\lexA^GMR15B03 also partially suppresses the tumors and the increased myoblasts.

Co-expression of dia^HM05027 under the control of Scer\GAL4^ap-md544 also rescues most Scer\GAL4^ap-md544>psq^KK111691,Egfr^UAS.cBa individuals to adulthood; the adults are fertile and their only visible phenotype is wing blade defects

(Fereres et al., 2019)

Expression of psq^KK111691 massively enhances the overproliferation seen in wing discs expressing Egfr^Scer\UAS.cBa under the control of Scer\GAL4^ap-md544. The discs grow as multilayered masses. The larvae do not enter pupariation and the discs continue to grow, often filling the anterior half of the larva. The cells lose their apical-basal polarity and cells metastasise into internal organs including the gut and malpighian tubules. The basement membranes have degraded in these discs. Mutant epithelial cells are intermingled with wild type mesenchymal cells that resemble the adepithelial cells. Expression of psq^KK111691 and Egfr^Scer\UAS.cBa specifically in the wing pouch under the control of Scer\GAL4^nub.PU produces epithelial hyperplasia but no tumors. The proliferation of both the epithelial and mesenchymal cells in the tumor is rapid, with most of the mesenchymal proliferation taking place close to the epithelial layer.

Expression of dpp^JF01371 partially suppresses the growth of the massive wing disc tumors seen when Egfr^Scer\UAS.cBa and psq^KK111691 are expressed under the control of Scer\GAL4^ap-md544.

Expression of wg^GD5007 partially suppresses the growth of the massive wing disc tumors seen when Egfr^Scer\UAS.cBa and psq^KK111691 are expressed under the control of Scer\GAL4^ap-md544.

Expression of trol^GL01153 suppresses the growth of the massive wing disc tumors seen when Egfr^Scer\UAS.cBa and psq^KK111691 are expressed under the control of Scer\GAL4^ap-md544. Apicobasal polarity and tissue organisation is restored to the epithelial cells.

Expression of trol^KK105502 suppresses the growth of the massive wing disc tumors seen when Egfr^Scer\UAS.cBa and psq^KK111691 are expressed under the control of Scer\GAL4^ap-md544. Apicobasal polarity and tissue organisation is restored to the epithelial cells.

Ablating adepithelial cells through expression of rpr^{Ecol\lexAop.cHa} under the control of Ecol\lexA^GMR15B03 suppresses the neoplastic transformation seen when psq^KK111691 and Egfr^Scer\UAS.cBa are co-expressed under the control of Scer\GAL4^ap-md544.

Expression of pan^ΔN.Scer\UAS suppresses the growth of the massive wing disc tumors seen when Egfr^Scer\UAS.cBa and psq^KK111691 are expressed under the control of Scer\GAL4^ap-md544. Apicobasal polarity and tissue organisation is restored to the epithelial cells.

Expression of Mad^KK109050 does not suppress the growth of the massive wing disc tumors seen when Egfr^Scer\UAS.cBa and psq^KK111691 are expressed under the control of Scer\GAL4^ap-md544. Apicobasal polarity and tissue organisation is restored to the epithelial cells.

Expression of Mad^{dsRNA.IVS.13xEcol\lexAop} in adepithelial cells under the control of Ecol\lexA^GMR15B03 suppresses the neoplastic transformation seen when psq^KK111691 and Egfr^Scer\UAS.cBa are co-expressed under the control of Scer\GAL4^ap-md544.

(Herranz et al., 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v106404

P{KK111691}VIE-260B

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

psq^KK111691

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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