FlyBase Allele Report: Dmel\dia[HM05027]

General Information

Symbol

Dmel\dia^HM05027

Species

D. melanogaster

Name

FlyBase ID

FBal0239359

Feature type

allele

Associated gene

Dmel\dia

Associated Insertion(s)

Carried in Construct

P{TRiP.HM05027}

Also Known As

UAS-dia-RNAi, UAS-diaRNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Perkins et al., 2009)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Perkins et al., 2009)

Mutagen

in vitro construct

(Perkins et al., 2009)

Progenitor genotype

Carried in construct

P{TRiP.HM05027}

Cytology

Description

UAS regulatory sequences drive expression of an inverted repeat.

(Perkins et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-HM05027

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased cell number | embryonic stage, with Scer\GAL4^srp.Hemo

(Belyaeva et al., 2022)

Phenotype Manifest In

embryonic/larval plasmatocyte | decreased number | embryonic stage, with Scer\GAL4^srp.Hemo

(Belyaeva et al., 2022)

germ band, with Scer\GAL4^srp.Hemo

(Belyaeva et al., 2022)

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

Egfr^UAS.cBa, Scer\GAL4^ap-md544, dia^HM05027, psq^KK111691 has neoplasia | larval stage phenotype, non-suppressible by Nrg^HMS01638, Scer\GAL4^ap-md544

(Fereres et al., 2019)

Suppressor of

Statement

Reference

dia^HM05027, Scer\GAL4^ap-md544 is a suppressor of neoplasia | larval stage phenotype of Egfr^UAS.cBa, Scer\GAL4^ap-md544, psq^KK111691

(Fereres et al., 2019)

dia^HM05027, Scer\GAL4^ap-md544 is a suppressor of increased cell number | larval stage phenotype of Egfr^UAS.cBa, Scer\GAL4^ap-md544, psq^KK111691

(Fereres et al., 2019)

dia^HM05027, Scer\GAL4^ap-md544 is a suppressor of abnormal cell polarity | larval stage phenotype of Egfr^UAS.cBa, Scer\GAL4^ap-md544, psq^KK111691

(Fereres et al., 2019)

dia^HM05027, Scer\GAL4^ap-md544 is a suppressor of abnormal cell migration | larval stage phenotype of Egfr^UAS.cBa, Scer\GAL4^ap-md544, psq^KK111691

(Fereres et al., 2019)

dia^HM05027, Scer\GAL4^ap-md544 is a suppressor of increased mortality during development phenotype of Egfr^UAS.cBa, Scer\GAL4^ap-md544, psq^KK111691

(Fereres et al., 2019)

dia^HM05027, Scer\GAL4^ap-md544 is a suppressor of partially lethal - majority live phenotype of Egfr^UAS.cBa, Scer\GAL4^ap-md544, psq^KK111691

(Fereres et al., 2019)

dia^HM05027, Scer\GAL4^ptc-559.1 is a suppressor of increased cell number | larval stage phenotype of Ret^MEN2B.UAS, Scer\GAL4^ptc-559.1

(Fereres et al., 2019)

Scer\GAL4^ptc-559.1/dia^HM05027 is a suppressor of increased mortality during development phenotype of Ret^MEN2B.UAS

(Fereres et al., 2019)

Scer\GAL4^ptc-559.1/dia^HM05027 is a suppressor of partially lethal - majority die phenotype of Ret^MEN2B.UAS

(Fereres et al., 2019)

Other

Statement

Reference

Egfr^UAS.cBa, Scer\GAL4^ap-md544, dia^HM05027, psq^KK111691 has fertile phenotype

(Fereres et al., 2019)

Egfr^UAS.cBa, Scer\GAL4^ap-md544, dia^HM05027, psq^KK111691 has visible | adult stage phenotype

(Fereres et al., 2019)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

Egfr^UAS.cBa, Scer\GAL4^ap-md544, dia^HM05027, psq^KK111691 has wing disc phenotype, non-suppressible by Nrg^HMS01638, Scer\GAL4^ap-md544

(Fereres et al., 2019)

Suppressor of

Statement

Reference

dia^HM05027, Scer\GAL4^ap-md544 is a suppressor of wing disc phenotype of Egfr^UAS.cBa, Scer\GAL4^ap-md544, psq^KK111691

(Fereres et al., 2019)

dia^HM05027, Scer\GAL4^ap-md544 is a suppressor of embryonic/larval tracheal section phenotype of Egfr^UAS.cBa, Scer\GAL4^ap-md544, psq^KK111691

(Fereres et al., 2019)

dia^HM05027, Scer\GAL4^ptc-559.1 is a suppressor of anterior-posterior compartment boundary of the wing disc phenotype of Ret^MEN2B.UAS, Scer\GAL4^ptc-559.1

(Fereres et al., 2019)

dia^HM05027/Scer\GAL4^Cg.PA is a suppressor of fat cell | third instar larval stage phenotype of Scer\GAL4^Cg.PA, shot^GL01286

(Sun et al., 2019)

dia^HM05027/Scer\GAL4^Cg.PA is a suppressor of endoplasmic reticulum | third instar larval stage phenotype of Scer\GAL4^Cg.PA, shot^GL01286

(Sun et al., 2019)

dia^HM05027/Scer\GAL4^Cg.PA is a suppressor of nucleus | third instar larval stage phenotype of Scer\GAL4^Cg.PA, shot^GL01286

(Sun et al., 2019)

NOT Suppressor of

Statement

Reference

dia^HM05027, Scer\GAL4^ap-md544 is a non-suppressor of wing disc phenotype of Egfr^UAS.cBa, Nrg^HMS01638, Scer\GAL4^ap-md544, psq^KK111691

(Fereres et al., 2019)

Other

Statement

Reference

Egfr^UAS.cBa, Scer\GAL4^ap-md544, dia^HM05027, psq^KK111691 has wing blade phenotype

(Fereres et al., 2019)

Ret^MEN2B.UAS, Scer\GAL4^ptc-559.1, dia^HM05027 has wing vein phenotype

(Fereres et al., 2019)

Additional Comments

Genetic Interactions

Statement

Reference

All larvae co-expressing psq^KK111691 and Egfr^UAS.cBa under the control of Scer\GAL4^ap-md544 during larval stages (using Gal80[ts] for the temporal regulation of expression) wing discs tumors, associated with higher numbers of myoblasts and a more extensive/branched trachea than normal across the disc. These phenotypes are partially suppressed by dia^HM05027 co-expression, but not further suppressed by dia^HM05027, Nrg^HMS01638 combined co-expression.

The co-expression of dia^HM05027 under the control of Scer\GAL4^ap-md544 rescues most Scer\GAL4^ap-md544>psq^KK111691,Egfr^UAS.cBa individuals to adulthood; these adults are fertile and their only visible phenotype is wing blade defects

(Fereres et al., 2019)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

28541

y¹ v¹; P{TRiP.HM05027}attP2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

dia^HM05027

Name Synonyms

Secondary FlyBase IDs

References (14)

Report Sections

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