FlyBase Allele Report: Scer\GAL4[srp.Hemo]

General Information

Symbol

Scer\GAL4^srp.Hemo

Species

S. cerevisiae

Name

FlyBase ID

FBal0197807

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{srp.Hemo-GAL4}

Also Known As

srp-Gal4, srpHemo-Gal4, srpHemoGAL4, serpent-GAL4, srp.Hemo-GAL4, srp(hemo)Gal4, serpentGal4

Allele class

Mutagen

in vitro construct - regulatory fusion

Nature of the Allele

Allele class

Mutagen

in vitro construct - regulatory fusion

(Bruckner et al., 2004)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Carried in construct

P{srp.Hemo-GAL4}

Cytology

Nature of the lesion

Statement

Reference

Expression of a GAL4 driver is under the control of 2 genomic sequences from upstream of srp amplified with AGGGTACCCTACTGCTTCCCACTCTAAGACTTCCAGTTTTAGGCTACG (sense) and GGAATTCGGCAATGCCCCACCCCTTGGCTGGACGG (antisense) as well as CGCGGTACCCAGCGGGAGCAACAGGATCAAATGCAGCAGCG (sense) and CGCGGTACCTATGGGATCCGTGCTGGGGTAGTGCTCGTAGAGC (antisense).

(Bruckner et al., 2004)

Allele components

Product class / Tool use(s)

Regulatory region(s)

srp

Encoded product / tool

GAL4

binary expression system - driver

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

embryonic hemocyte

(Kuraishi et al., 2009)

embryonic stage 9

embryonic hemocyte anlage

(Bruckner et al., 2004)

embryonic stage 9 -- 17

procrystal cell

(Bruckner et al., 2004)

embryonic stage 10 -- 15

embryonic hemocyte

(Bruckner et al., 2004)

head mesoderm derived embryonic hemocyte

(Bruckner et al., 2004)

embryonic stage 10 -- 17

plasmatocyte

(Bruckner et al., 2004)

crystal cell

(Bruckner et al., 2004)

embryonic stage 12 -- 15

macrophage

(Weavers et al., 2016)

embryonic stage 12 -- 17

embryonic hemocyte

(Martinek et al., 2008)

embryonic stage 14 -- 17

embryonic/larval hemocyte

(Wood et al., 2006)

embryonic stage 15 -- 17

embryonic/larval hemocyte

(Bruckner et al., 2004)

embryonic stage 16 -- 17

embryonic hemocyte

(Bunt et al., 2011)

larval stage

embryonic/larval pericardial cell

(Pendse et al., 2013)

embryonic/larval garland cell

(Pendse et al., 2013)

embryonic/larval hemocyte

(Pendse et al., 2013, Matova and Anderson, 2006)

embryonic/larval lymph gland

(Matova and Anderson, 2006)

first instar larval stage -- third instar larval stage

embryonic/larval pericardial cell

Comment: from 48 hr AEL

(Das et al., 2008)

Additional Information

Statement

Reference

Drives expression in hemocytes and pericardial cells (nephrocytes) from embryonic stage 9 through all larval stages.

(FlyBase Curators, 2017)

Scer\GAL4^srp.Hemo drives expression in nephrocytes and hemocytes

(Pendse et al., 2013)

Scer\GAL4^srp.Hemo drives expression in all hemocytes and macrophages at all embryonic stages, as well as in the head mesoderm that gives rise to hemocytes beginning at embryonic stage 9.

(Bruckner et al., 2004)

Marker for

embryonic hemocyte

(Bunt et al., 2011, Martinek et al., 2008)

embryonic/larval pericardial cell

(Das et al., 2008)

hemocyte

(Siekhaus et al., 2010, Kuraishi et al., 2009)

Reflects expression of

srp

(Weavers et al., 2016, Pendse et al., 2013, Bunt et al., 2011, Kuraishi et al., 2009, Das et al., 2008, Martinek et al., 2008, Wood et al., 2006, Bruckner et al., 2004)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell migration | embryonic stage 12, with C1GalTA^GD1019

(Valoskova et al., 2019)

abnormal cell migration | embryonic stage 12, with Qsox1^KK101108

(Valoskova et al., 2019)

abnormal cell number | embryonic stage 12, with C1GalTA^GD1019

(Valoskova et al., 2019)

abnormal cell number | embryonic stage 12, with Qsox1^KK101108

(Valoskova et al., 2019)

abnormal cell polarity, with spas^UAS.cTa

(Stramer et al., 2010)

decreased cell number | embryonic stage 12, with C1GalTA^KK101417

(Valoskova et al., 2019)

decreased cell number | embryonic stage 12, with mrva^KK103883

(Valoskova et al., 2019)

lethal, with Pvr^{ΔC.UAS.Tag:SS(hEFNB1),Tag:HA}

(Bruckner et al., 2004)

lethal - all die before end of pupal stage, with Rac1^V12.UAS

(Ninov et al., 2007)

some die during pupal stage, with Rac1^V12.UAS

(Ninov et al., 2007)

viable, with Pvr^{λ.UASp.Tag:MYC}

(Bruckner et al., 2004)

Phenotype Manifest In

cytoskeleton, with spas^UAS.cTa

(Stramer et al., 2010)

embryonic/larval hemocyte | third instar larval stage, with Rho1^N19.UAS

(Bausek and Zeidler, 2014)

embryonic/larval hemocyte | third instar larval stage, with Rho1^V14.UAS

(Bausek and Zeidler, 2014)

embryonic anterior Malpighian tubule, with Scer\GAL4^crq.PO, vkg^KK111668

(Bunt et al., 2010)

embryonic hemocyte, with hid^Ala5.UAS

(Bruckner et al., 2004, Bruckner et al., 2004)

embryonic hemocyte, with Itgbn^NIG.1762R

(Nonaka et al., 2013)

embryonic hemocyte, with Pvr^{ΔC.UAS.Tag:SS(hEFNB1),Tag:HA}

(Bruckner et al., 2004)

embryonic hemocyte, with Pvr^{λ.UASp.Tag:MYC}

(Bruckner et al., 2004)

embryonic hemocyte, with Ras85D^N17.UAS

(Bruckner et al., 2004)

embryonic hemocyte, with scb^NIG.8095R

(Nonaka et al., 2013)

filopodium, with Rho1^N19.UAS

(Bausek and Zeidler, 2014)

filopodium, with Rho1^V14.UAS

(Bausek and Zeidler, 2014)

germ band | embryonic stage 12, with C1GalTA^GD1019

(Valoskova et al., 2019)

germ band | embryonic stage 12, with C1GalTA^KK101417

(Valoskova et al., 2019)

germ band | embryonic stage 12, with mrva^KK103883

(Valoskova et al., 2019)

germ band | embryonic stage 12, with Qsox1^KK101108

(Valoskova et al., 2019)

hemocyte, with Rac1^V12.UAS

(Ninov et al., 2007)

hemocyte, with spas^UAS.cTa

(Stramer et al., 2010)

hemocyte | late embryonic stage, with sty^UAS.C.Tag:HA

(Bruckner et al., 2004)

larval ventral nerve cord | embryonic stage, with Rcom\RT^A.UAS

(Martinek et al., 2008)

macrophage | embryonic stage 12, with C1GalTA^GD1019

(Valoskova et al., 2019)

macrophage | embryonic stage 12, with C1GalTA^KK101417

(Valoskova et al., 2019)

macrophage | embryonic stage 12, with mrva^KK103883

(Valoskova et al., 2019)

macrophage | embryonic stage 12, with Qsox1^KK101108

(Valoskova et al., 2019)

microtubule, with spas^UAS.cTa

(Stramer et al., 2010)

yolk | embryonic stage 12, with C1GalTA^GD1019

(Valoskova et al., 2019)

yolk | embryonic stage 12, with GlcAT-P^MI05251

(Valoskova et al., 2019)

yolk | embryonic stage 12, with Qsox1^KK101108

(Valoskova et al., 2019)

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Scer\GAL4^srp.Hemo/BacA\p35^UAS.cHa is a suppressor of increased cell death | recessive phenotype of Pvr¹

(Bruckner et al., 2004)

Phenotype Manifest In

Suppressor of

Statement

Reference

dl^UASp.cMa, Scer\GAL4^srp.Hemo, dl¹ is a suppressor of hemocyte phenotype of Dif¹

(Matova and Anderson, 2006)

dl^UASp.cMa, Scer\GAL4^srp.Hemo, dl¹ is a suppressor of hemolymph phenotype of Dif¹

(Matova and Anderson, 2006)

Scer\GAL4^srp.Hemo/BacA\p35^UAS.cHa is a suppressor of embryonic hemocyte phenotype of Pvr¹

(Wood et al., 2006)

Ras85D^V12.UAS/Scer\GAL4^srp.Hemo is a suppressor | partially of macrophage | embryonic stage phenotype of Pvr¹

(Bruckner et al., 2004)

Scer\GAL4^srp.Hemo/Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)} is a suppressor | partially of embryonic hemocyte phenotype of Pvr¹

(Bruckner et al., 2004)

Scer\GAL4^srp.Hemo/BacA\p35^UAS.cHa is a suppressor of macrophage | embryonic stage phenotype of Pvr¹

(Bruckner et al., 2004)

Scer\GAL4^srp.Hemo/BacA\p35^UAS.cHa is a suppressor | partially of embryonic hemocyte phenotype of Pvr¹

(Bruckner et al., 2004)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^srp.Hemo/mrva^{UAS.Tag:FLAG,Tag:HA} rescues mrva^EP3102

(Valoskova et al., 2019)

C1GalTA^UAS.cFa/Scer\GAL4^srp.Hemo rescues C1GalTA^EY13370

(Fuwa et al., 2015)

drpr^UAS.cFa/Scer\GAL4^srp.Hemo rescues drpr^Δ5

(Fujita et al., 2012)

Scer\GAL4^srp.Hemo/drpr^Y949F.UAS rescues drpr^Δ5

(Fujita et al., 2012)

Vps16B^UAS.Tag:MYC/Scer\GAL4^srp.Hemo rescues Vps16B¹

(Akbar et al., 2011)

Itgbn^UAS.cNa/Scer\GAL4^srp.Hemo rescues Itgbn²

(Nagaosa et al., 2011)

chb^UAS.GFP/Scer\GAL4^srp.Hemo rescues chb²

(Stramer et al., 2010)

sn^UASp.EGFP/Scer\GAL4^srp.Hemo rescues Df(1)C128

(Zanet et al., 2009)

sn^{S52A.UASp.EGFP}/Scer\GAL4^srp.Hemo rescues Df(1)C128

(Zanet et al., 2009)

sn^{S52D.UASp.EGFP}/Scer\GAL4^srp.Hemo rescues Df(1)C128

(Zanet et al., 2009)

sn^{S52E.UASp.EGFP}/Scer\GAL4^srp.Hemo rescues Df(1)C128

(Zanet et al., 2009)

Partially rescues

sn^UASp.cZa/Scer\GAL4^srp.Hemo partially rescues Df(1)C128

(Zanet et al., 2009)

sn^UASp.EGFP/Scer\GAL4^srp.Hemo partially rescues sn^X2

(Zanet et al., 2009)

sn^{S52A.UASp.EGFP}/Scer\GAL4^srp.Hemo partially rescues sn^X2

(Zanet et al., 2009)

sn^{S52E.UASp.EGFP}/Scer\GAL4^srp.Hemo partially rescues sn^X2

(Zanet et al., 2009)

Pvr^{λ.UASp.Tag:MYC}/Scer\GAL4^srp.Hemo partially rescues Pvr¹

(Bruckner et al., 2004)

Fails to rescue

Scer\GAL4^srp.Hemo/drpr^Y858F.UAS fails to rescue drpr^Δ5

(Fujita et al., 2012)

prtp^UAS.cKa/Scer\GAL4^srp.Hemo fails to rescue prtp^Δ1

(Kuraishi et al., 2009)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

78565

y¹ w^*; P{srp.Hemo-GAL4}2, P{UAS-GFP.U}3

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Scer\GAL4^srp.Hemo

Name Synonyms

Secondary FlyBase IDs

References (43)

Report Sections

Open Close