FlyBase Allele Report: Dmel\cher[KK107518]

General Information

Symbol

Dmel\cher^KK107518

Species

D. melanogaster

Name

FlyBase ID

FBal0234984

Feature type

allele

Associated gene

Dmel\cher

Associated Insertion(s)

Carried in Construct

P{KK107518}

Also Known As

UAS-cher RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

(Keleman et al., 2009.8.5)

Progenitor genotype

Carried in construct

P{KK107518}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Keleman et al., 2009.8.5)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-KK107518

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of distal myopathy 4

CEA

(Ader et al., 2022)

model of myofibrillar myopathy 5

CEA

(Ader et al., 2022)

model of myopathy

CEA

(González-Morales et al., 2017)

CEA with cher^JF02077

(González-Morales et al., 2017)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased cell number | embryonic stage, with Scer\GAL4^srp.Hemo

(Belyaeva et al., 2022)

flightless, with Scer\GAL4^Mef2.PU

(González-Morales et al., 2017)

increased size | adult stage, with Scer\GAL4^{Hand.ΔVM.Switch}, cher^JF02077

(Ader et al., 2022)

Phenotype Manifest In

adult cardial cell, with Scer\GAL4^{Hand.ΔVM.Switch}, cher^JF02077

(Ader et al., 2022)

adult heart, with Scer\GAL4^{Hand.ΔVM.Switch}

(Ader et al., 2022)

adult heart, with Scer\GAL4^{Hand.ΔVM.Switch}, cher^JF02077

(Ader et al., 2022)

adult heart chamber 1 | adult stage, with Scer\GAL4^{Hand.ΔVM.Switch}, cher^JF02077

(Ader et al., 2022)

embryonic/larval hypodermal muscle cell | third instar larval stage, with Scer\GAL4^Mef2.PR

(Wójtowicz et al., 2015)

embryonic/larval plasmatocyte | decreased number | embryonic stage, with Scer\GAL4^srp.Hemo

(Belyaeva et al., 2022)

filamentous actin | embryonic stage, with Scer\GAL4^srp.Hemo

(Belyaeva et al., 2022)

germ band, with Scer\GAL4^srp.Hemo

(Belyaeva et al., 2022)

indirect flight muscle cell | adult stage, with Scer\GAL4^Mef2.PU

(González-Morales et al., 2017)

indirect flight muscle cell | adult stage, with Scer\GAL4^Mef2.PU, cher^JF02077

(González-Morales et al., 2017)

sarcomere | adult stage, with Scer\GAL4^{Hand.ΔVM.Switch}, cher^JF02077

(Ader et al., 2022)

sarcomere | adult stage, with Scer\GAL4^Mef2.PU

(González-Morales et al., 2017)

sarcomere | adult stage, with Scer\GAL4^Mef2.PU, cher^JF02077

(González-Morales et al., 2017)

Detailed Description

Statement

Reference

The expression of cher^KK107518 under the control of Scer\GAL4^Mef2.PU leads to a flightless phenotype, associated with a severe decrease in the number of recognizable sarcomeres in adult indirect flight muscles, as compared to controls; the severe defects in sarcomere structure are enhanced upon additional expression of cher^JF02077.

(González-Morales et al., 2017)

Expression of cher^KK107518 driven by Scer\GAL4^Mef2.PR leads to irregular sarcomeric actin patterns in third instar larval muscle.

(Wójtowicz et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Scer\GAL4^srp.Hemo, cher^KK107518 has decreased cell number | embryonic stage phenotype, non-enhanceable by TM4SF^KK111096/Scer\GAL4^srp.Hemo

(Belyaeva et al., 2022)

NOT suppressed by

Statement

Reference

Scer\GAL4^srp.Hemo, cher^KK107518 has decreased cell number | embryonic stage phenotype, non-suppressible by TM4SF^KK111096/Scer\GAL4^srp.Hemo

(Belyaeva et al., 2022)

Enhancer of

Statement

Reference

cher^KK107518/Scer\GAL4^srp.Hemo is an enhancer of decreased cell number | embryonic stage phenotype of Scer\GAL4^srp.Hemo, TM4SF^KK111096

(Belyaeva et al., 2022)

Suppressor of

Statement

Reference

cher^KK107518, Scer\GAL4^T80 is a suppressor of abnormal cell migration | somatic clone phenotype of Ras85D^V12.UAS, Scer\GAL4^T80, scrib¹

(Külshammer and Uhlirova, 2013)

NOT Suppressor of

Statement

Reference

cher^KK107518, Scer\GAL4^T80 is a non-suppressor of neoplasia | somatic clone phenotype of Ras85D^V12.UAS, Scer\GAL4^T80, scrib¹

(Külshammer and Uhlirova, 2013)

cher^KK107518, Scer\GAL4^T80 is a non-suppressor of abnormal mitotic cell cycle | somatic clone phenotype of Ras85D^V12.UAS, Scer\GAL4^T80, scrib¹

(Külshammer and Uhlirova, 2013)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Scer\GAL4^srp.Hemo, cher^KK107518 has embryonic/larval plasmatocyte | embryonic stage | decreased number phenotype, non-enhanceable by TM4SF^KK111096/Scer\GAL4^srp.Hemo

(Belyaeva et al., 2022)

Scer\GAL4^srp.Hemo, cher^KK107518 has germ band phenotype, non-enhanceable by TM4SF^KK111096/Scer\GAL4^srp.Hemo

(Belyaeva et al., 2022)

NOT suppressed by

Statement

Reference

Scer\GAL4^srp.Hemo, cher^KK107518 has embryonic/larval plasmatocyte | embryonic stage | decreased number phenotype, non-suppressible by TM4SF^KK111096/Scer\GAL4^srp.Hemo

(Belyaeva et al., 2022)

Scer\GAL4^srp.Hemo, cher^KK107518 has germ band phenotype, non-suppressible by TM4SF^KK111096/Scer\GAL4^srp.Hemo

(Belyaeva et al., 2022)

Enhancer of

Statement

Reference

cher^KK107518/Scer\GAL4^srp.Hemo is an enhancer of embryonic/larval plasmatocyte | embryonic stage | decreased number phenotype of Scer\GAL4^srp.Hemo, TM4SF^KK111096

(Belyaeva et al., 2022)

cher^KK107518/Scer\GAL4^srp.Hemo is an enhancer of germ band phenotype of Scer\GAL4^srp.Hemo, TM4SF^KK111096

(Belyaeva et al., 2022)

NOT Suppressor of

Statement

Reference

cher^KK107518, Scer\GAL4^T80 is a non-suppressor of eye-antennal disc | somatic clone phenotype of Ras85D^V12.UAS, Scer\GAL4^T80, scrib¹

(Külshammer and Uhlirova, 2013)

Other

Statement

Reference

Scer\GAL4^Mef2.PR, cher^KK107518, l(2)efl^GD11341 has embryonic/larval hypodermal muscle cell | third instar larval stage phenotype

(Wójtowicz et al., 2015)

Scer\GAL4^Mef2.PR, cher^KK107518, l(2)efl^UASp.Venus has embryonic/larval hypodermal muscle cell | third instar larval stage phenotype

(Wójtowicz et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of cher^KK107518 and l(2)efl^GD11341 driven by Scer\GAL4^Mef2.PR leads to myofibril misalignment and severely affected sarcomeric actin patterns in third instar larval muscle.

Co-expression of l(2)efl^{Scer\UAS.P\T.T:Avic\GFP-YFP.Venus} partially rescues irregular actin patterns in muscle with cher^KK107518 driven by Scer\GAL4^Mef2.PR in third instar larvae.

(Wójtowicz et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v107451

P{KK107518}VIE-260B

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

cher^KK107451

(González-Morales et al., 2017)

cher^KK107518

Name Synonyms

Secondary FlyBase IDs

References (13)

Report Sections