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General Information
Symbol
Dmel\Ras85DN17.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0048924
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-rasN17, UAS-RasN17, UAS-RasDN, UAS-DrasN17, UAS-Ras1N17, UAS-Dras1N17, UAS-Ras85D.N17, RasN17, rasdn, UAS-Ras185DN17
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

A dominant-negative form of Ras85D is expressed under the control of UASt regulatory sequences.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In

glial cell & antennal disc, with Scer\GAL4hs.PB

larval optic lobe & glial cell | somatic clone | cell non-autonomous, with Scer\GAL4αTub84B.PP

medulla cortex & glial cell | somatic clone | cell non-autonomous, with Scer\GAL4αTub84B.PP

photoreceptor cell & axon & eye disc, with Scer\GAL4bi-omb-Gal4

sensory neuron & axon & embryo, with Scer\GAL4repo

subretinal glial cell & eye disc, with Scer\GAL4bi-omb-Gal4

subretinal glial cell & larval optic stalk, with Scer\GAL4bi-omb-Gal4

Detailed Description
Statement
Reference

Expressing Ras85DN17.UAS under the control of Scer\GAL4Bx-MS1096 results in a reduced wing size and loss of vein material.

Expression of Ras85DN17.UAS under the control of Scer\GAL4Su(H).GBE (in combination with tub-Gal80[ts] to restrict expression to the adult stage) leads to a decrease in the cell size of enteroblasts in adult guts when compared to controls.

Expression of Ras85DN17.Scer\UAS specifically in adult glia (under the control of Scer\GAL4repo and Scer\GAL80ts.αTub84B) generates a mild glial engulfment phenotype, resulting in a slight delay in phagocytosis of axonal debris after axotomy.

Expression of Ras85DN17.Scer\UAS under the control of Scer\GAL4esg-NP5130 and Scer\GAL80ts.αTub84B has a mild reducing effect on intestinal stem cells/epithelial enteroblasts cell numbers.

Subperineurial expression of Ras85DN17.Scer\UAS under the control of Scer\GAL4Gli-rL82 in a wild-type genetic background has only a minor effect on larval peripheral nerves.

Increased gut length is observed in flies expressing Ras85DN17.Scer\UAS under the control of Scer\GAL4Myo31DF-NP0001.

Guts of flies expressing Ras85DN17.Scer\UAS in enterocytes under the control of Scer\GAL4Myo31DF-NP0001 and Scer\GAL80ts.αTub84B shrink less upon infection with Erwinia carotovora carotovora 15 (Ecc15). After a recovery phase of 2 or 8 days, the guts are 25% longer than their wild-type counterparts.

Expression of Ras85DN17.Scer\UAS under the control of Scer\GAL4bi.PU results in the loss of the central part of wing vein L4.

Expression of Ras85DN17.Scer\UAS in the larvae under the control of Scer\GAL4Act5C.Switch.PR does not result in lethality. Both male and female life-span not reduced significantly when Ras85DV12.Scer\UAS is expressed in adult flies.

Expression of Ras85DN17.Scer\UAS in the wing blade using Scer\GAL4MD-638 results in loss of sections of wing vein.

The number of fusion competent myoblasts is increased in stage 11 embryos that express Ras85DV12.Scer\UAS under the control of Scer\GAL4twi.PB.

Expression of Ras85DN17.Scer\UAS under the control of Scer\GAL4btl.PS results tracheal branching defects in embryos.

There is a large increase in primordial germ cell numbers when the dominant negative Ras85DN17.Scer\UAS is expressed in somatic gonadal cells by Scer\GAL4C587.

Expression of Ras85DN17.Scer\UAS, under the control of Scer\GAL4en-e16E, in the posterior compartment cells of embryos results in apoptosis of these cells, beginning at stage 12. The increased apoptosis occurs mainly at the front of the posterior compartment. Overall compartment size is reduced. Surviving cells are larger than normal.

Expression of Ras85DN17.Scer\UAS under the control of Scer\GAL4amn-c651, results in pupae and adults that are larger than wild-type controls. These flies also undergo delayed pupariation about 48 hr after wild-type controls, which is mostly due to a prolonged second-instar stage. Expression of Ras85DN17.Scer\UAS under the control of Scer\GAL4amn-X8 causes an increase in pupal length. Expression of Ras85DN17.Scer\UAS under the control of Scer\GAL4amn-c651 has no effect on prothoracic gland cell size.

Hemocytes are mildly enlarged in about one third of Ras85DN17.Scer\UAS; Scer\GAL4srp.Hemo embryos.

Somatic clones of Ras85DN17.Scer\UAS; Scer\GAL4αTub84B.PP neurons in the posterior of the optic lobe result in failure of scaffold axons to extend toward glial destinations. When the scaffold axons are absent, glia stall prior to migrating into the neuropile regions. The number of apoptotic cells is increased in the cortical areas next to the resulting glia-deficient regions.

Migration of ganglionic tracheal branches is abberant in Ras85DN17.Scer\UAS; Scer\GAL4bs-23.26 embryos. Migration of around half of these branches stalls outside the ventral nerve cord. A smaller proportion are grossly misrouted (8%) or cross the midline (4%).

Flies expressing Ras85DN17.Scer\UAS under the control of Scer\GAL4GMR.PU have reduced and roughened eyes.

When Ras85DN17.Scer\UAS is driven by Scer\GAL4hs.PB and heatshocked between 14 and 15 hours after puparium formation, a severe reduction is seen in numbers of antennal glia.

Ras85DN17.Scer\UAS; Scer\GAL4prd.RG1 embryos do not have obvious cuticle phenotypes.

Ras85DN17.Scer\UAS with Scer\GAL4pnr-MD237 or Scer\GAL4426 does not affect wing disc development. Ras85DN17.Scer\UAS with Scer\GAL4ap-md544 or Scer\GAL4tsh-md621 causes severe reduction in notum tissue in the 3rd instar wing disc; however, no transformation of notum to wing fate is observed in these discs. Ras85DN17.Scer\UAS with Scer\GAL4Gug-AGiR causes reduction in notum tissue in the 3rd instar wing disc. Ras85DN17.Scer\UAS; Scer\GAL4Ubx-lac1-Gal4 adults exhibit wing duplication in the posterior compartment and partial loss of notum and/or hinge. Occasionally pharate adults die within the pupal case, with severe loss of notum tissue. In larvae of this genotype, the wing discs show altered morphology, particularly overgrowth in the posterior hinge and mesonotum. Marker analysis indicates formation a new wing pouch complete with the DV boundary, and that pattern duplication is associated with loss of notum, confirming notum/hinge-to-wing transformations. Ras85DN17.Scer\UAS; Scer\GAL4ap-md544 third instar wing discs show a severe reduction in the notum size.

Expression of Ras85DN17.Scer\UAS in embryonic glia, under the control of Scer\GAL4repo, causes PNS patterning defects in hemisegments. Embryos show incomplete peripheral glial ensheathment of nerves and stalled glia that are able to extend cytoplasmic processes, although to a lesser extent than in wild type. In hemisegments that are moderately affected, sensory axons can be misdirected and nerves in the CNS-PNS transition zone show incorrect fasciculation-bundling. In severely-affected hemisegments, there is a loss of sensory neurons and those remaining have misplaced cell bodies and misdirected sensory axons. Additionally, motor neuron patterning is disrupted.

Hemocytes in mutant embryos (Ras85DN17.Scer\UAS driven by Scer\GAL4gcm.PP) fail to enter the tail.

When Ras85DN17.Scer\UAS is driven by Scer\GAL4ftz.ng, no midline crossovers are seen in the pCC/MP2 pathway axons. When Ras85DN17.Scer\UAS is driven by Scer\GAL4ftz.ng, only 2% of embryos exhibit midline crossovers are seen in the pCC/MP2 pathway axons.

Expression of Ras85DN17.Scer\UAS driven by Scer\GAL4332.3 does not affect either the dorsal cuticle or amnioserosa morphology.

Expression of Ras85DN17.Scer\UAS, under the control of Scer\GAL4C380 increases the number of type I synaptic boutons compared to wild-type controls. This increase is indistinguishable from the increase in bouton number observed in flies where Ras85DScer\UAS.cKa expression is driven by Scer\GAL4C380.

Expression of Ras85DN17.Scer\UAS under the control of Scer\GAL4slbo.2.6 moderately affects posterior and dorsal border cell migration.

Ras85DN17.Scer\UAS; P{sgcm-GAL4}151 embryos exhibit a dramatic increase in apoptosis of longitudinal (interface) glial cells.

Expression of Ras85DN17.Scer\UAS under the control of Scer\GAL4dpp.blk1 inhibits furrow reincarnation along the lateral margins, but not furrow birth at the posterior of the eye disc.

Imaginal disc cells expressing Ras85DN17.Scer\UAS under the control of Scer\GAL4Act5C.PP are smaller than wild-type cells. The cells have longer apparent doubling times. The cells mix well with neighbouring wild-type cells. Pyknotic nuclei, indicating cell autonomous cell death, are seen. The proportion of cells in G1 is increased and the proportion of cells in S phase is decreased. Clones expressing Ras85DN17.Scer\UAS under the control of Scer\GAL4Act5C.PP fails to make veins in adult wings.

Expression of Ras85DN17.Scer\UAS under the control of Scer\GAL4hs.PB (using heat shock) results in many first instar larvae with mild puckering of the dorsal cuticle.

The anterior crossvein is missing in flies expressing Ras85DN17.Scer\UAS under the control of Scer\GAL4dpp.blk1.

Expression of Ras85DN17.Scer\UAS under the control of Scer\GAL448Y in the developing midgut has no effect on the migration of the endodermal midgut cells.

The number of glial cells in the optic stalk is reduced and they fail to migrate into the eye disc in approximately 10% of eye discs expressing Ras85DN17.Scer\UAS under the control of Scer\GAL4bi-omb-Gal4. In these cases the photoreceptor cells are able to grow axons and these axons orient their growth towards the posterior of the eye disc as in wild type. However, the axons then remain stuck in the eye disc and are unable to exit into the optic stalk. In 40% of cases, eye discs expressing Ras85DN17.Scer\UAS under the control of Scer\GAL4bi-omb-Gal4 contain a small number of glial cells (3 to 20). These glial cells are found very close to the optic stalk at the very posterior end of the eye disc where they remain in a cluster. The cell bodies of the glial cells are small and do not extend much anteriorly. Axons are able to exit into the optic stalk normally in these eye discs.

Scer\GAL4sli.PS-mediated expression in wild type embryos does not result in an abnormal CNS phenotype. Scer\GAL4sim.PS-mediated expression in Ras85D deficient embryos causes slight fusion of commissures.

The presence of Scer\GAL4c306 causes a mild delay in the migration of border cells in egg chambers. Border cell phenotype is enhanced in Scer\GAL4c306; P{UAS-Ras85D.N17}; Ras85DΔ17b/Ras85D+ flies.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
NOT Enhanced by
Statement
Reference
Suppressed by
Statement
Reference
NOT suppressed by
Statement
Reference
NOT Enhancer of
Suppressor of
NOT Suppressor of
Other
Phenotype Manifest In
Enhanced by
NOT Enhanced by
Statement
Reference
Suppressed by
Statement
Reference
NOT suppressed by
Statement
Reference
NOT Enhancer of
Suppressor of
NOT Suppressor of
Other
Additional Comments
Genetic Interactions
Statement
Reference

The wing defects induced by the expression of Ras85DN17.UAS under the control of Scer\GAL4Bx-MS1096 (smaller wing and loss of L3-5 vein material are: fully suppressed by the co-expression of either rlD334N.UAS.cKa or rlR80S.D334N.UAS, even leading to extra vein material (more severe in the latter, with darker pigmentation); partially suppressed by the co-expression of rlR80S.UAS (complete suppression in L3, partial suppression in L4 or L5); not suppressed by the co-expression of rlWT.UAS.

Co-expression of Ras85DN17.Scer\UAS and PvrCA.Scer\UAS under the control of Scer\GAL4esg-NP5130 and Scer\GAL80ts.αTub84B significantly abrogates the PvrCA.Scer\UAS dysplastic phenotype, indicating that Ras85D is a downstream signaling component in the Pvr-dependent regulation of intestinal homeostasis.

Expression of Ras85DN17.Scer\UAS in motor neurons under the control of Scer\GAL4unspecified suppresses the increased synaptic growth and transmitter release observed at the neuromuscular junction in comt6 Ca-P60AKum170 larvae.

Co-expression of Bap170C1.Scer\UAS enhances the loss of wing vein L4 that is seen in flies expressing Ras85DN17.Scer\UAS under the control of Scer\GAL4bi.PU, such that there is a loss of the entire L4 vein distal to the posterior crossvein. The distal end of wing vein L3 is missing in these flies and a reduction in wing surface area and occasional notching of the wing margin is also seen.

Expression of Ras85DN17.Scer\UAS moderately suppresses the glial neoplasia seen in third instar larvae when btl::EgfrScer\UAS.T:λ\cI-DD and Pi3K92EScer\UAS.T:Hsap\MYC,T:Hsap\CAAX are co-expressed under the control of Scer\GAL4repo.PU.

The loss of wing vein by overexpression of Ras85DN17.Scer\UAS under the control of Scer\GAL4MD-638 is enhanced by osa308/+.

Denticle belt fusions in the cuticles of rho7M43; ru1 double homozygous embryos are enhanced by Ras85DN17.Scer\UAS; Scer\GAL4prd.RG1.

The reduction in notum size seen in the wing discs of Ras85DN17.Scer\UAS; Scer\GAL4ap-md544 third instar larvae is partially suppressed by BacA\p35Scer\UAS.cHa.

Co-expressing BacA\p35Scer\UAS.cHa with Ras85DN17.Scer\UAS under the control of Scer\GAL4Act5C.PP completely suppresses the Ras85DN17.Scer\UAS cell death phenotype and increases the number of cells per clone. The doubling time defect is only partially rescued. 40 hours after induction, clones in imaginal discs co-expressing Ras85DN17.Scer\UAS and BacA\p35Scer\UAS.cHa under the control of Scer\GAL4Act5C.PP are 23% smaller than clones expressing BacA\p35Scer\UAS.cHa alone under the control of Scer\GAL4Act5C.PP. Clones expressing both dmScer\UAS.cZa and Ras85DN17.Scer\UAS under the control of Scer\GAL4Act5C.PP in clones in the imaginal discs have a similar cell size and cell cycle profiles as those of cells expressing dmScer\UAS.cZa alone under the control of Scer\GAL4Act5C.PP. The elongated doubling time of cells expressing Ras85DN17.Scer\UAS under the control of Scer\GAL4Act5C.PP is partially rescued by co-expression of dmScer\UAS.cZa.

Xenogenetic Interactions
Statement
Reference

The additional co-expression of Ras85DN17.Scer\UAS does not modify the fully penetrant rough eye phenotype resulting from the co-expression of HPV18\E6Scer\UAS.T:Hsap\MYC and Hsap\UBE3AScer\UAS.cRa under the control of Scer\GAL4GMR.PU.

Complementation and Rescue Data
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Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (8)
Reported As
Symbol Synonym
Name Synonyms
Secondary FlyBase IDs
    References (66)