FlyBase Allele Report: Dmel\pbl[EP3415]

General Information

Symbol

Dmel\pbl^EP3415

Species

D. melanogaster

Name

FlyBase ID

FBal0101236

Feature type

allele

Associated gene

Dmel\pbl

Associated Insertion(s)

P{EP}pbl^EP3415

Carried in Construct

Also Known As

EP(3)3415

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Prokopenko et al., 1999)

Progenitor genotype

Associated Insertion(s)

P{EP}pbl^EP3415

Cytology

Description

The P{EP} element is inserted within pbl 5' UTR.

(Kraut et al., 2001)

P{EP} insertion at -10bp relative to the start of the transcription unit.

(Abdelilah-Seyfried et al., 2000)

P{EP} insertion 457bp upstream of the AUG start codon.

(Prokopenko et al., 1999)

Allele components

Component

Use(s)

Inserted element

P{EP}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3L:7,911,910..7,911,910 [-]

(Raymond et al., 2004, Tseng and Hariharan, 2002, Gene Disruption Project members, 2001-, Kraut et al., 2001, Kraut et al., 2001, Abdelilah-Seyfried et al., 2000, Prokopenko et al., 1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | larval stage, with Scer\GAL4^elav-C155

(Kraut et al., 2001)

visible, with Scer\GAL4^en-e16E

(Tseng and Hariharan, 2002)

visible, with Scer\GAL4^ey.PH

(Tseng and Hariharan, 2002)

visible, with Scer\GAL4^GMR.PU

(Raymond et al., 2004)

visible, with Scer\GAL4^hs.2sev

(Tseng and Hariharan, 2002)

visible, with Scer\GAL4^sca-537.4

(Abdelilah-Seyfried et al., 2000)

wild-type, with Scer\GAL4^prd.RG1

(Prokopenko et al., 1999)

Phenotype Manifest In

anterior fascicle & synapse | supernumerary, with Scer\GAL4^elav-C155

(Kraut et al., 2001)

eye, with Scer\GAL4^ey.PH

(Tseng and Hariharan, 2002)

eye, with Scer\GAL4^GMR.PU

(Raymond et al., 2004)

eye, with Scer\GAL4^hs.2sev

(Tseng and Hariharan, 2002)

eye | temperature conditional

(Reiter et al., 2006)

interommatidial bristle | temperature conditional

(Reiter et al., 2006)

ommatidium, with Scer\GAL4^hs.2sev

(Tseng and Hariharan, 2002)

scutellar bristle, with Scer\GAL4^sca-537.4

(Abdelilah-Seyfried et al., 2000)

thecogen cell | ectopic, with Scer\GAL4^sca-537.4

(Abdelilah-Seyfried et al., 2000)

tormogen cell, with Scer\GAL4^sca-537.4

(Abdelilah-Seyfried et al., 2000)

trichogen cell | ectopic, with Scer\GAL4^sca-537.4

(Abdelilah-Seyfried et al., 2000)

wing vein, with Scer\GAL4^en-e16E

(Tseng and Hariharan, 2002)

Detailed Description

Statement

Reference

Expression of pbl^EP3415 under the control of Scer\GAL4^GMR.PF at 25[o]C exhibit severely disorganized eyes and 40% of the animals display necrotic foci and yellowish loss of pigment at the center of the eye. These flies also have an overall glazed irregular appearance, loss of interommatidial bristles, holes in the lens and a severe reduction in the number of photoreceptors.

Expression of pbl^EP3415 under the control of Scer\GAL4^GMR.PF at room temperature generates a weak phenotype.

(Reiter et al., 2006)

Expression of pbl^EP3415 under the control of Scer\GAL4^GMR.PU results in a rough eye phenotype.

(Raymond et al., 2004)

Expression of pbl^EP3415 under the control of Scer\GAL4^ey.PH results in eyes that are strongly reduced in size. Flies expressing pbl^EP3415 under the control of Scer\GAL4^hs.2sev have rough eyes. Flies expressing pbl^EP3415 under the control of Scer\GAL4^en-e16E have a few incomplete wing veins.

(Tseng and Hariharan, 2002)

Mutant larvae expressing pbl^EP3415 under the control of Scer\GAL4^elav-C155 show complex phenotypes in the ISNs. This phenotype is characterised primarily by extra synaptic and axonal branches, some of which cross segment borders.

(Kraut et al., 2001)

Expression of pbl^EP3415 under the control of Scer\GAL4^sca-537.4 results in the loss of several scutellar macrochaetae. Sensory organs with no socket and one shaft, one socket and two shafts and rarely, one socket, one shaft and two sheath cells are seen.

(Abdelilah-Seyfried et al., 2000)

Over expression driven by Scer\GAL4^prd.RG1 does not result in a detectable phenotype.

(Prokopenko et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Scer\GAL4^sca-537.4, pbl^EP3415 has visible phenotype, non-enhanceable by H[+]/H^E21

(Abdelilah-Seyfried et al., 2000)

Scer\GAL4^sca-537.4, pbl^EP3415 has visible phenotype, non-enhanceable by N[+]/N^55e11

(Abdelilah-Seyfried et al., 2000)

Suppressed by

Statement

Reference

Scer\GAL4^ey.PH, pbl^EP3415 has visible phenotype, suppressible by CycE^UAS.cLa, Scer\GAL4^ey.PH

(Tseng and Hariharan, 2002)

NOT suppressed by

Statement

Reference

Scer\GAL4^sca-537.4, pbl^EP3415 has visible phenotype, non-suppressible by H[+]/H^E21

(Abdelilah-Seyfried et al., 2000)

Scer\GAL4^sca-537.4, pbl^EP3415 has visible phenotype, non-suppressible by N[+]/N^55e11

(Abdelilah-Seyfried et al., 2000)

Enhancer of

Statement

Reference

pbl^EP3415, Scer\GAL4^GMR.PU is an enhancer of visible phenotype of Cdc42^N17.UAS, Scer\GAL4^GMR.PU

(Raymond et al., 2004)

NOT Enhancer of

Statement

Reference

pbl^EP3415, Scer\GAL4^GMR.PU is a non-enhancer of visible phenotype of Ras85D^N17.UAS, Scer\GAL4^GMR.PU

(Raymond et al., 2004)

NOT Suppressor of

Statement

Reference

pbl^EP3415, Scer\GAL4^GMR.PU is a non-suppressor of visible phenotype of Ras85D^N17.UAS, Scer\GAL4^GMR.PU

(Raymond et al., 2004)

Other

Statement

Reference

Rac1^N17.UAS, Scer\GAL4^GMR.PU, pbl^EP3415 has lethal | pupal stage phenotype

(Raymond et al., 2004)

RacGAP84C^GAP.UAS, Scer\GAL4^GMR.PU, pbl^EP3415 has lethal | pupal stage phenotype

(Raymond et al., 2004)

Df(3R)rn20, Scer\GAL4^GMR.PU, pbl^EP3415 has lethal | pupal stage phenotype

(Raymond et al., 2004)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, faf^EP381, pbl^EP3415 has eye phenotype, suppressible by Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PF

(Reiter et al., 2006)

Scer\GAL4^GMR.PF, faf^EP381, pbl^EP3415 has ommatidium phenotype, suppressible by Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PF

(Reiter et al., 2006)

Scer\GAL4^ey.PH, pbl^EP3415 has eye phenotype, suppressible by CycE^UAS.cLa, Scer\GAL4^ey.PH

(Tseng and Hariharan, 2002)

Enhancer of

Statement

Reference

Scer\GAL4^GMR.PF/pbl^EP3415 is an enhancer of eye phenotype of Scer\GAL4^GMR.PF, faf^EP381

(Reiter et al., 2006)

Scer\GAL4^GMR.PF/pbl^EP3415 is an enhancer of ommatidium phenotype of Scer\GAL4^GMR.PF, faf^EP381

(Reiter et al., 2006)

pbl^EP3415, Scer\GAL4^GMR.PU is an enhancer of eye phenotype of Cdc42^N17.UAS, Scer\GAL4^GMR.PU

(Raymond et al., 2004)

NOT Enhancer of

Statement

Reference

pbl^EP3415, Scer\GAL4^GMR.PU is a non-enhancer of eye phenotype of Ras85D^N17.UAS, Scer\GAL4^GMR.PU

(Raymond et al., 2004)

Scer\GAL4^sca-537.4/pbl^EP3415 is a non-enhancer of phenotype of N^55e11

(Abdelilah-Seyfried et al., 2000)

Scer\GAL4^sca-537.4/pbl^EP3415 is a non-enhancer of phenotype of H^E21

(Abdelilah-Seyfried et al., 2000)

NOT Suppressor of

Statement

Reference

pbl^EP3415, Scer\GAL4^GMR.PU is a non-suppressor of eye phenotype of Ras85D^N17.UAS, Scer\GAL4^GMR.PU

(Raymond et al., 2004)

Scer\GAL4^sca-537.4/pbl^EP3415 is a non-suppressor of phenotype of H^E21

(Abdelilah-Seyfried et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of CycE^Scer\UAS.cLa suppresses the small eye phenotype caused by expression of pbl^EP3415 under the control of Scer\GAL4^ey.PH.

(Tseng and Hariharan, 2002)

Xenogenetic Interactions

Statement

Reference

The synergistic effect of pbl^EP3415 and faf^EP381 can be overcome by co-expression with Hsap\UBE3A^Scer\UAS.cRa (all under the control of Scer\GAL4^GMR.PF.

(Reiter et al., 2006)

Complementation and Rescue Data

Fails to complement

pbl^S008320

(Prokopenko et al., 2000)

pbl^S054203a

(Prokopenko et al., 2000)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Excision of the P{lacW} is accompanied by a reversion to wild-type.

(Prokopenko et al., 1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

EP(3)3415

(Abdelilah-Seyfried et al., 2000, Prokopenko et al., 2000)

pbl^EP3415

Name Synonyms

Secondary FlyBase IDs

References (10)

Report Sections

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