FlyBase Allele Report: Dmel\faf[EP381]

General Information

Symbol

Dmel\faf^EP381

Species

D. melanogaster

Name

FlyBase ID

FBal0119253

Feature type

allele

Associated gene

Dmel\faf

Associated Insertion(s)

P{EP}faf^EP381

Carried in Construct

Also Known As

EP(3)0381, EP381

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Watts et al., 2003, Pena-Rangel et al., 2002, DiAntonio et al., 2001, Gene Disruption Project members, 2001-, Kraut et al., 2001, Kraut et al., 2001, Abdelilah-Seyfried et al., 2000)

Progenitor genotype

Associated Insertion(s)

P{EP}faf^EP381

Cytology

Description

The P{EP} element is inserted within the first intron, upstream of the entire coding region.

(Kraut et al., 2001)

P{EP} insertion at -572bp relative to the start of the transcription unit.

(Abdelilah-Seyfried et al., 2000)

Allele components

Component

Use(s)

Inserted element

P{EP}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3R:31,777,287..31,777,287 [+]

(Uthaman et al., 2008, Collins et al., 2006, Reiter et al., 2006, Watts et al., 2003, Pena-Rangel et al., 2002, DiAntonio et al., 2001, Gene Disruption Project members, 2001-, Kraut et al., 2001, Kraut et al., 2001, Abdelilah-Seyfried et al., 2000)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Huntington's disease

modeled by Hsap\HTT^{128Q.1-336.UAS}

(Kaltenbach et al., 2007)

exacerbates Alzheimer's disease 1

modeled by Hsap\APP^{Aβ42.UAS.Tag:SS(rPENK)}

(Lee et al., 2012)

modeled by Hsap\APP^UAS.cUa

(Lee et al., 2012)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult abdominal sensillum & trichogen cell, with Scer\GAL4^sca-537.4

(Abdelilah-Seyfried et al., 2000)

adult mushroom body, with Scer\GAL4^Tab2-201Y

(Watts et al., 2003)

anterior fascicle & bouton, with Scer\GAL4^elav-C155

(Kraut et al., 2001)

anterior fascicle & synapse, with Scer\GAL4^elav-C155

(Kraut et al., 2001)

anterior fascicle & synapse | supernumerary, with Scer\GAL4^elav-C155

(Kraut et al., 2001)

bouton, with Scer\GAL4^elav-C155

(Collins et al., 2006)

bouton | increased number, with Scer\GAL4^elav-C155

(DiAntonio et al., 2001)

dorsocentral bristle, with Scer\GAL4^sca-537.4

(Abdelilah-Seyfried et al., 2000)

eye, with Scer\GAL4^GMR.PF

(Reiter et al., 2006)

giant fiber neuron, with Scer\GAL4^shot-OK307

(Uthaman et al., 2008)

larval intersegmental nerve, with Scer\GAL4^elav-C155

(Kraut et al., 2001)

mesothoracic tergotrochanter muscle motor neuron, with Scer\GAL4^shot-OK307

(Uthaman et al., 2008)

neuromuscular junction, with Scer\GAL4^elav-C155

(Collins et al., 2006)

ommatidium, with Scer\GAL4^GMR.PF

(Reiter et al., 2006)

scutellar bristle, with Scer\GAL4^sca-537.4

(Abdelilah-Seyfried et al., 2000)

synapse, with Scer\GAL4^elav-C155

(DiAntonio et al., 2001, Kraut et al., 2001)

synapse, with Scer\GAL4^shot-OK307

(Uthaman et al., 2008)

synapse | increased number, with Scer\GAL4^elav-C155

(DiAntonio et al., 2001)

trichogen cell, with Scer\GAL4^sca-537.4

(Abdelilah-Seyfried et al., 2000)

wing sensillum | ectopic, with Scer\GAL4^ap-md544

(Pena-Rangel et al., 2002)

Detailed Description

Statement

Reference

faf^EP381 overexpression mutants exhibit defects in the giant fiber-tergotrochanteral motorneuron synapse, such as axonal overgrowth.

(Uthaman et al., 2008)

Neural expression of faf^EP381, under the control of Scer\GAL4^elav-C155 leads to an increased number of synaptic boutons and branches and an increased synaptic span.

(Collins et al., 2006)

Expression of faf^EP381 under the control of Scer\GAL4^GMR.PF at room temperature results in smooth glassy eyes (due to fusion of ommatidia) that are also moderately reduced in size.

(Reiter et al., 2006)

Expression of faf^EP381 under the control of Scer\GAL4^Tab2-201Y results in misguidance and mushroom body overgrowth phenotypes.

(Watts et al., 2003)

Flies expressing faf^EP381 under the control of Scer\GAL4^ap-md544 have ectopic bristles on the dorsal surface of the wing blade.

(Pena-Rangel et al., 2002)

When faf^EP381 is driven by Scer\GAL4^elav-C155, a marked expansion in the size and complexity is seen in the synapses. There is an increase in both the number of synaptic boutons and in the synaptic span. There is also an increase in the number of synaptic branches. When the evoked excitatory junctional potentials (EJPs) of faf^EP381, Scer\GAL4^elav-C155 embryos are examined, a marked reduction is seen in their amplitudes. A large decrease in quantal content is also seen, and the frequency of spontaneous mEJPs is also reduced.

(DiAntonio et al., 2001)

Mutant larvae expressing faf^EP381 under the control of Scer\GAL4^elav-C155 have ISN neuron pathfinding defects. Mutant larvae expressing faf^EP381 under the control of Scer\GAL4^elav-C155 have excessive or ectopic synapses. Mutant larvae expressing faf^EP381 under the control of Scer\GAL4^elav-C155 have reduced or abnormal synapses. They have abnormal ISN synaptic branches and a reduced number of boutons.

(Kraut et al., 2001)

In the dorsal muscle region of mutant larvae expressing faf^EP381 under the control of Scer\GAL4^elav-C155, the synapses at the second branchpoint and terminal arbor have abnormal structures, with a spray of extra branches emanating from the terminal arbor.

(Kraut et al., 2001)

Expression of faf^EP381 under the control of Scer\GAL4^sca-537.4 results in loss of external support cells of the scutellar and dorsocentral macrochaetae without loss of the sheath cell. Shafts with a branched tip are seen. On the abdomen, dot-like shaft morphology is seen.

(Abdelilah-Seyfried et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Scer\GAL4^sca-537.4, faf^EP381 has visible phenotype, non-enhanceable by N[+]/N^55e11

(Abdelilah-Seyfried et al., 2000)

NOT suppressed by

Statement

Reference

Scer\GAL4^sca-537.4, faf^EP381 has visible phenotype, non-suppressible by N[+]/N^55e11

(Abdelilah-Seyfried et al., 2000)

Suppressor of

Statement

Reference

faf^EP381, Scer\GAL4^GMR.PU is a suppressor of visible phenotype of Hsap\HTT^{128Q.1-336.UAS}, Scer\GAL4^GMR.PU

(Kaltenbach et al., 2007)

Other

Statement

Reference

dpp^hr4/dpp[+], faf^EP381 has lethal | maternal effect phenotype

(Stinchfield et al., 2012)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PF, faf^EP381 has eye phenotype, enhanceable by Scer\GAL4^GMR.PF/pbl^EP3415

(Reiter et al., 2006)

Scer\GAL4^GMR.PF, faf^EP381 has ommatidium phenotype, enhanceable by Scer\GAL4^GMR.PF/pbl^EP3415

(Reiter et al., 2006)

Suppressed by

Statement

Reference

Scer\GAL4^elav-C155, faf^EP381 has neuromuscular junction phenotype, suppressible by wnd¹/wnd²

(Collins et al., 2006)

Scer\GAL4^elav-C155, faf^EP381 has bouton phenotype, suppressible by wnd¹/wnd²

(Collins et al., 2006)

Scer\GAL4^GMR.PF, faf^EP381, pbl^EP3415 has eye phenotype, suppressible by Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PF

(Reiter et al., 2006)

Scer\GAL4^GMR.PF, faf^EP381, pbl^EP3415 has ommatidium phenotype, suppressible by Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PF

(Reiter et al., 2006)

Scer\GAL4^GMR.PF, faf^EP381 has eye phenotype, suppressible by Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PF

(Reiter et al., 2006)

NOT Enhancer of

Statement

Reference

faf^EP381/Scer\GAL4^sca-537.4 is a non-enhancer of phenotype of N^55e11

(Abdelilah-Seyfried et al., 2000)

Suppressor of

Statement

Reference

faf^EP381, Scer\GAL4^GMR.PU is a suppressor of eye phenotype of Hsap\HTT^{128Q.1-336.UAS}, Scer\GAL4^GMR.PU

(Kaltenbach et al., 2007)

Other

Statement

Reference

dpp^hr4/dpp[+], faf^EP381 has embryonic/first instar larval cuticle | maternal effect phenotype

(Stinchfield et al., 2012)

Scer\GAL4^pnr-MD237, faf^EP381, pnr^MD237/pnr[+] has scutellum phenotype

(Pena-Rangel et al., 2002)

Scer\GAL4^pnr-MD237, faf^EP381, pnr^MD237/pnr[+] has adult abdomen phenotype

(Pena-Rangel et al., 2002)

Scer\GAL4^pnr-MD237, faf^EP381, pnr^MD237 has scutellum phenotype

(Pena-Rangel et al., 2002)

Scer\GAL4^pnr-MD237, faf^EP381, pnr^MD237 has adult abdomen phenotype

(Pena-Rangel et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

Animals derived from homozygous faf^EP381 females mated to dpp^hr4/+ males show strong lethality (only 26% of the expected number survive). The embryos have a ventralised cuticle phenotype.

(Stinchfield et al., 2012)

wnd¹/wnd² mutants suppress the synapse phenotype of faf^EP381, under the control of Scer\GAL4^elav-C155.

(Collins et al., 2006)

The faf^EP381 (Scer\GAL4^GMR.PF) overexpression phenotype is strongly enhanced by co-expression with pbl^EP3415 as the ommatidial field is reduced to approximately 30% of normal in Scer\GAL4^GMR.PF-driven pbl^EP3415 and faf^EP381 flies.

(Reiter et al., 2006)

pnr^MD237/+ adults expressing faf^EP381 under the control of Scer\GAL4^pnr-MD237 have a slight cleft in the abdominal dorsal midline and a reduced scutellum.

(Pena-Rangel et al., 2002)

Xenogenetic Interactions

Statement

Reference

The synergistic effect of pbl^EP3415 and faf^EP381 can be overcome by co-expression with Hsap\UBE3A^Scer\UAS.cRa (all under the control of Scer\GAL4^GMR.PF.

Co-expression of Hsap\UBE3A^Scer\UAS.cRa with faf^EP381 (both under the control of Scer\GAL4^GMR.PF) partially suppresses the smooth eye phenotype induced by expression of faf^EP381 alone.

(Reiter et al., 2006)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

6414

y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{5xglBS-lacZ.38-1}TPN1; P{EP}faf^EP381

Notes on Origin

Discoverer

Selected as: a P{EP} insertion line that modifies the pnr^MD237/+ phenotype when expressed using Scer\GAL4^pnr-MD237.

(Pena-Rangel et al., 2002)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

EP(3)0381

(Abdelilah-Seyfried et al., 2000)

EP381

(Kaltenbach et al., 2007, Watts et al., 2003)

FAF^EP381

(Lee et al., 2012)

faf^EP0381

faf^EP381

(Yagi et al., 2013, Stinchfield et al., 2012)

Name Synonyms

Secondary FlyBase IDs

References (14)

Report Sections

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