G1 phase & external sensory organ precursor cell IIIb, with Scer\GAL4neur-P72
The expression of CycEScer\UAS.cLa under the control of Scer\GAL4elav-C155 suppresses the decreased neuroblast/neuroblast daughter divisions observed in wor4/Df(2L)ED1054 transheterozygous stage 14 embryos.
Co-expression of CycEScer\UAS.cLa fails to suppress the decreased nucleolar size phenotype seen in secondary neuroblasts in larval brains expressing bansponge.Scer\UAS.T:Disc\RFP under the control of Scer\GAL4insc-Mz1407.
The co-expression of CycEScer\UAS.cLa does not change the number of neuroblasts per brain lobe seen in larval brains expressing both bansponge.Scer\UAS.T:Disc\RFP and NScer\UAS.T:SV5\V5,T:Zzzz\His6 under the control of Scer\GAL4insc-Mz1407.
Expression of CycEScer\UAS.cLa suppresses the cell proliferation defects seen in the wing disc when caupScer\UAS.T:Ivir\HA1 is expressed under the control of either Scer\GAL4nub.PU or Scer\GAL4ap-md544 (restricted to 16 hours prior the third instar larval stage onwards using Scer\GAL80ts.αTub84B).
Expression of CycEScer\UAS.cLa suppresses the cell proliferation defects seen in the wing disc when mirrScer\UAS.cMa is expressed under the control of Scer\GAL4nub.PU (restricted to 16 hours prior the third instar larval stage onwards using Scer\GAL80ts.αTub84B).
Expression of CycEScer\UAS.cLa suppresses the cell proliferation defects seen in the wing disc when araScer\UAS.cGa is expressed under the control of Scer\GAL4nub.PU (restricted to 16 hours prior the third instar larval stage onwards using Scer\GAL80ts.αTub84B).
Expression of caupScer\UAS.T:Ivir\HA1 suppresses the eye disc overgrowth seen when ykiScer\UAS.cUa is expressed under the control of Scer\GAL4salm.EPv. This suppression is partially reverted upon co-expression of CycEScer\UAS.cLa.
Expression of CycEScer\UAS.cLa suppresses the wing loss and formation of ectopic notum tissue seen when araScer\UAS.cGa is expressed in the prospective wing pouch under the control of Scer\GAL4MD-638.
The small eye phenotype caused by expression of kibraScer\UAS.cBa under the control of Scer\GAL4ey.PH is partially rescued by co-expression of either thEP3723 or CycEScer\UAS.cLa. Simultaneous co-expression of both thEP3723 and CycEScer\UAS.cLa almost completely rescues the small eye phenotype caused by expression of kibraScer\UAS.cBa under the control of Scer\GAL4ey.PH.
Expression of stgScer\UAS.cNa and CycEScer\UAS.cLa (using the MARCM system under the control of Scer\GAL4tub.PU) fails to rescue the growth defects seen in Df(1)btd-Sp1 leg disc clones generated in second instar larvae.
Co-expression of geminindsRNA.Scer\UAS with CycEScer\UAS.cLa in 'flip out' clones in salivary glands does not rescue the block to DNA replication associated with the expression of CycEScer\UAS.cLa alone.
Expressing CycEScer\UAS.cLa under the control of Scer\GAL4en-e16E in Df(3L)XR38 embryos reduces the level of apoptosis in embryonic posterior compartment cells by 59% compared to when CycEScer\UAS.cLa is expressed in a wild-type background. Likewise, Scer\GAL4en-e16E>CycEScer\UAS.cLa expression in a W05014 background reduces apoptosis in these cells by 65%.
Coexpression of Egfr::toract.Scer\UAS and CycEScer\UAS.cLa, under the control of Scer\GAL4en-e16E, blocks CycEScer\UAS.cLa-induced apoptosis in the embryonic posterior compartment cells. There is a reduction in compartment size, similar to that seen when only Egfr::toract.Scer\UAS is expressed, and cells are of a size similar to that seen when only CycEScer\UAS.cLa is expressed. The same phenotype occurs when Ras85DV12.Scer\UAS and CycEScer\UAS.cLa are coexpressed.
Embryos that coexpress EgfrDN.Scer\UAS and CycEScer\UAS.cLa, under the control of Scer\GAL4en-e16E, show approximately double the amount of apoptosis in cells of posterior compartments relative to expressing CycEScer\UAS.cLa alone.
Expression of CycEScer\UAS.cLa, under the control of Scer\GAL4en-e16E, in spi1 embryos increases cell death in the posterior compartment, relative to expression of CycEScer\UAS.cLa in a wild-type background.
Co-expression of Cdk2Scer\UAS.T:Hsap\MYC with CycEScer\UAS.cLa driven by Scer\GAL4GMR.PF blocks some photoreceptor differentiation in the third instar eye disc. The phenotype of these discs is largely unaffected by upd1Scer\UAS.cCa.
When CycEScer\UAS.cLa is expressed under the control of either Scer\GAL4ey.PB or Scer\GAL4ey.PH in NDN.Scer\UAS eye discs, rescue of the NDN.Scer\UAS mitotic phenotype is seen; eye discs are larger and contain more proliferating cells.
When CycEScer\UAS.cLa (driven by Scer\GAL4mf9) is added to Df(2L)Prl/prd4, (partially rescued by a weakly expressing prdmf9 line), the accessory gland phenotype is reduced, and male fertility is restored. When CycEScer\UAS.cLa (driven by Scer\GAL4prd.3.1) is added to Df(2L)Prl/prd4, however, no rescue is seen.
When CycEScer\UAS.cLa is driven by Scer\GAL4bi-omb-Gal4, in a eyaunspecified background, glial cells in the optic stalk undergo excessive proliferation. However these glial cells never enter the eye disc.
Expression of both Tsc1Scer\UAS.cTa and gigScer\UAS.cTa in the eye under the control of Scer\GAL4ey.PH results in a much smaller eye than normal. Coexpression of CycEScer\UAS.cLa fully suppresses the phenotype.
stgScer\UAS.cNa and CycEScer\UAS.cLa bypass the effects of RbfScer\UAS.cDa on cell cycle phasing, cell numbers and cell size when they are both co-expressed with RbfScer\UAS.cDa using Scer\GAL4en-e16E.
Coexpression of CycEScer\UAS.cLa and BacA\p35Scer\UAS.cHa, under the control of Scer\GAL4en-e16E, in the posterior compartment cells of embryos, blocks the apoptosis of these cells that occurs when CycEScer\UAS.cLa is expressed alone. The posterior compartments of these embryos show a further increase in the number of cells per posterior compartment compared to those expressing only CycEScer\UAS.cLa (77 vs 59). The Scer\GAL4en-e16E>CycEScer\UAS.cLa, BacA\p35Scer\UAS.cHa posterior compartments do not overgrow due to an extreme reduction in average cell size.