Third instar larvae expressing nejEP1179 under the control of Scer\GAL4Pxn.PS have a decreased number of sessile hemocytes. The number of hemocytes in circulation is also decreased. The dorsal sessile hemocyte department is disrupted. Sessile hemocytes accumulate along the dorsal vessel but do not spread through the cuticle. The lymph gland is not enlarged. Most hemocytes appear wild type in shape, although a few larger cells can also be detected.
nejEP1179/nejTA57 or nejEP1179/nejS342 transheterozygotes are semi-viable as third instar larvae. These larvae are developmentally delayed by approximately 1 day and are sluggish. Homozygous larvae show no change in synaptic structure or function compared to wild type. nejEP1179/nejTA57 or nejEP1179/nejS342 larvae show an approximately 50% decrease in quantal content without any change in quantal size at the neuromuscular junction. No change in the average muscle resting potential or in the average muscle input resistance is seen. Overexpression of nejEP1179 under the control of Scer\GAL4elav.PLu (in nejEP1179 heterozygotes) results in a decrease in presynaptic transmitter release at the neuromuscular junction, without any change quantal size. There is a significant decrease in the number of boutons at the neuromuscular junction. Overexpression of nejEP1179 under the control of both Scer\GAL4Mhc.PW and Scer\GAL4elav.PLu (in nejEP1179 heterozygotes) results in a decrease in presynaptic transmitter release at the neuromuscular junction, without any change quantal size or muscle physiological parameters. Synaptic ultrastructure at neuromuscular boutons appears normal. There is a significant (although moderate) increase in the number of boutons at the neuromuscular junction of muscles 4, 6 and 7 compared to wild type in nejEP1179/nejS342, nejEP1179/nejQ7 or nejEP1179/nejTA57 larvae. No alteration in axon guidance in the embryonic central nervous system is seen.