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General Information
Symbol
Dmel\nejEP1179
Species
D. melanogaster
Name
FlyBase ID
FBal0241474
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

P{EP} element insertion approximately 3kb upstream of the nej open reading frame, in the 5' UTR. The orientation of the P{EP} element is such that the 3' end of the P{EP} element is proximal to the nej open reading frame, allowing overexpression of nej under the control of the Scer\UAS regulatory sequences present in the P{EP} element.

Insertion components
P{EP}nejEP1179
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Third instar larvae expressing nejEP1179 under the control of Scer\GAL4Pxn.PS have a decreased number of sessile hemocytes. The number of hemocytes in circulation is also decreased. The dorsal sessile hemocyte department is disrupted. Sessile hemocytes accumulate along the dorsal vessel but do not spread through the cuticle. The lymph gland is not enlarged. Most hemocytes appear wild type in shape, although a few larger cells can also be detected.

Expression of nejEP1179 under the control of Scer\GAL4GMR.PF results in a small eye phenotype.

nejEP1179/nejTA57 or nejEP1179/nejS342 transheterozygotes are semi-viable as third instar larvae. These larvae are developmentally delayed by approximately 1 day and are sluggish. Homozygous larvae show no change in synaptic structure or function compared to wild type. nejEP1179/nejTA57 or nejEP1179/nejS342 larvae show an approximately 50% decrease in quantal content without any change in quantal size at the neuromuscular junction. No change in the average muscle resting potential or in the average muscle input resistance is seen. Overexpression of nejEP1179 under the control of Scer\GAL4elav.PLu (in nejEP1179 heterozygotes) results in a decrease in presynaptic transmitter release at the neuromuscular junction, without any change quantal size. There is a significant decrease in the number of boutons at the neuromuscular junction. Overexpression of nejEP1179 under the control of both Scer\GAL4Mhc.PW and Scer\GAL4elav.PLu (in nejEP1179 heterozygotes) results in a decrease in presynaptic transmitter release at the neuromuscular junction, without any change quantal size or muscle physiological parameters. Synaptic ultrastructure at neuromuscular boutons appears normal. There is a significant (although moderate) increase in the number of boutons at the neuromuscular junction of muscles 4, 6 and 7 compared to wild type in nejEP1179/nejS342, nejEP1179/nejQ7 or nejEP1179/nejTA57 larvae. No alteration in axon guidance in the embryonic central nervous system is seen.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

nejEP1179 fails to complement nejTA57 and nejS342 for synaptic transmission defects.

Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (6)
Reported As
Name Synonyms
Secondary FlyBase IDs
  • FBal0104983
  • FBal0141926
References (12)