FlyBase Allele Report: Dmel\L[2]

General Information

Symbol

Dmel\L²

Species

D. melanogaster

Name

FlyBase ID

FBal0005882

Feature type

allele

Associated gene

Dmel\L

Associated Insertion(s)

roo{}L-2

Carried in Construct

Key Links

Allele class

neomorphic allele - genetic evidence

Mutagen

spontaneous

Nature of the Allele

Allele class

neomorphic allele - genetic evidence

(Davis and MacIntyre, 1988)

Mutagen

spontaneous

Progenitor genotype

Associated Insertion(s)

roo{}L-2

Cytology

Description

roo element inserted in the 3' intergenic region downstream of L. This roo{}L-2 insertion contains a number of sequence alterations (622bp duplication, 5bp deletion, a nucleotide change that results in the formation of an ATG) compared to roo{}Mohr (an insertion found in a number of dominant L mutant alleles isolated around 1920, and also in the ft¹ chromosome isolated during the same time period), and probably arose by alteration of it. Reversion analysis suggests that the roo{}L-2 insertion is largely responsible for the dominant L² eye phenotype. The chromosome also carries a number of other natural transposon insertions in the L region (jockey{}Mohr and opus{}Mohr), however these insertions are found in a number of dominant L mutant alleles isolated around 1920, and also in the ft¹ chromosome isolated during the same time period, suggesting that the additional insertions are probably not related to the dominant L mutant eye phenotype.

(Son and Choi, 2020)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell death | dominant | larval stage

(Singh et al., 2006)

lethal (with Df(2R)trix)

(Singh et al., 2005)

lethal | recessive

(Singh et al., 2005)

viable (with Df(2R)ED2354)

(Son and Choi, 2020)

visible | adult stage | dominant

(Zhang et al., 2010)

visible | dominant

(Son and Choi, 2020, Singh et al., 2005, Chern and Choi, 2002, Davis and MacIntyre, 1988)

Phenotype Manifest In

eye

(Son and Choi, 2020, Singh et al., 2011, Zhang et al., 2010, Chern and Choi, 2002)

eye | ventral

(Singh et al., 2006, Singh et al., 2005)

eye disc

(Singh et al., 2011)

eye disc | ventral

(Singh et al., 2006, Singh et al., 2005)

ommatidium

(Singh et al., 2005)

Detailed Description

Statement

Reference

L² heterozygotes exhibit a selective loss of ventral eye in the larval eye imaginal disc and adult eye.

(Singh et al., 2011)

L²/+ flies show a loss of the ventral eye cells both at the eye disc and adult eye level. Use of the TUNEL assay reveals that cell death occurs predominantly in the ventral eye margin of the L²/+ eye disc but not in the dorsal half of the eye.

(Singh et al., 2006)

Heterozygotes show loss of almost all ventral ommatidia; apart from a few cells along the ventral eye margin, nearly all ommatidia in L²/+ flies are dorsal in fate. This loss of ventral eye pattern is also seen in heterozygous eye discs.

(Singh et al., 2005)

Heterozygotes have a nick near the anterior midline of the eye and the overall size of the eye is reduced.

(Chern and Choi, 2002)

L² flies do not differ from wild type in speed of development.

(Dunn and Mossige, 1937)

Eyes of L²/+ as small as or smaller than L¹/L¹. L² homozygotes have tiny eyes and are poorly viable or completely lethal, depending on background.

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

L² has visible | dominant phenotype, enhanceable by rod^EP3408/Scer\GAL4^ey.PH

(Singh et al., 2005)

L² has visible | dominant phenotype, enhanceable by +/Df(2L)BSC7

(Singh et al., 2005)

L² has visible | dominant phenotype, enhanceable by Scer\GAL4^ey.PH/EP1350^EP1350

(Singh et al., 2005)

L² has visible | dominant phenotype, enhanceable by Scer\GAL4^ey.PH/EP1410^EP1410

(Singh et al., 2005)

L² has visible | dominant phenotype, enhanceable by EP3087^EP3087/Scer\GAL4^ey.PH

(Singh et al., 2005)

L² has visible | dominant phenotype, enhanceable by EP950^EP950/Scer\GAL4^ey.PH

(Singh et al., 2005)

L² has visible | dominant phenotype, enhanceable by Dad^EP3196/Scer\GAL4^ey.PH

(Singh et al., 2005)

L² has visible | dominant phenotype, enhanceable by ara^UAS.cGa/Scer\GAL4^ey.PH

(Singh et al., 2005)

L² has visible | dominant phenotype, enhanceable by Scer\GAL4^ey.PH/dally^EP3168

(Singh et al., 2005)

L² has visible | dominant phenotype, enhanceable by emc^EP3166/Scer\GAL4^ey.PH

(Singh et al., 2005)

L² has visible | dominant phenotype, enhanceable by emc^EP3614/Scer\GAL4^ey.PH

(Singh et al., 2005)

L² has visible | dominant phenotype, enhanceable by emc^EP3620/Scer\GAL4^ey.PH

(Singh et al., 2005)

L² has visible | dominant phenotype, enhanceable by emc^EP415/Scer\GAL4^ey.PH

(Singh et al., 2005)

L² has visible | dominant phenotype, enhanceable by esg⁰⁵⁷²⁹/esg[+]

(Singh et al., 2005)

L² has visible | dominant phenotype, enhanceable by hh^bar3/hh^bar3

(Singh et al., 2005)

L² has visible | dominant phenotype, enhanceable by Scer\GAL4^ey.PH/mirr^UAS.cMa

(Singh et al., 2005)

L² has visible | dominant phenotype, enhanceable by nej^EP1149/Scer\GAL4^ey.PH

(Singh et al., 2005)

L² has visible | dominant phenotype, enhanceable by aop^EP2500/Scer\GAL4^ey.PH

(Singh et al., 2005)

L² has visible | dominant phenotype, enhanceable by Scer\GAL4^ey.PH/nej^EP1179

(Singh et al., 2005)

L² has visible | dominant phenotype, enhanceable by sgg[+]/sgg¹

(Singh et al., 2005)

L² has visible | dominant phenotype, enhanceable by aop^EP598/Scer\GAL4^ey.PH

(Singh et al., 2005)

L² has visible | dominant phenotype, enhanceable by B[+]/Bar¹

(Singh et al., 2005)

NOT Enhanced by

Statement

Reference

L² has visible | dominant phenotype, non-enhanceable by PRAS40[+]/PRAS40^P17

(Son and Choi, 2020)

L² has visible | dominant phenotype, non-enhanceable by PRAS40[+]/PRAS40^KO

(Son and Choi, 2020)

L² has visible | dominant phenotype, non-enhanceable by PRAS40^RNAi.WIZ.UAS/Scer\GAL4^ey.PU

(Son and Choi, 2020)

L² has visible | dominant phenotype, non-enhanceable by phyl^JF03369/Scer\GAL4^ey.PU

(Son and Choi, 2020)

L² has visible | dominant phenotype, non-enhanceable by phyl^GD12579/Scer\GAL4^ey.PU

(Son and Choi, 2020)

L² has visible | dominant phenotype, non-enhanceable by Scer\GAL4^ey.PU/Achl^JF01976

(Son and Choi, 2020)

L² has increased cell death | larval stage | dominant phenotype, non-enhanceable by p53^UAS.Ex/Scer\GAL4^ey.PH

(Singh et al., 2006)

Suppressed by

Statement

Reference

L² has visible | dominant | adult stage phenotype, suppressible by hyx^EY06898

(Zhang et al., 2010)

L² has increased cell death | dominant | larval stage phenotype, suppressible by Diap1^UAS.cLa/Scer\GAL4^ey.PH

(Singh et al., 2006)

L² has increased cell death | dominant | larval stage phenotype, suppressible by Df(3L)H99/+

(Singh et al., 2006)

L² has increased cell death | dominant | larval stage phenotype, suppressible by Scer\GAL4^ey.PH/BacA\p35^UAS.cHa

(Singh et al., 2006)

L² has visible | dominant phenotype, suppressible by pygo^unspecified

(Singh et al., 2005)

L² has visible | dominant phenotype, suppressible by ush^UAS.cFa/Scer\GAL4^ey.PH

(Singh et al., 2005)

L² has visible | dominant phenotype, suppressible by CBP^EP1643/Scer\GAL4^ey.PH

(Singh et al., 2005)

L² has visible | dominant phenotype, suppressible by CG12054^EP3309/Scer\GAL4^ey.PH

(Singh et al., 2005)

L² has visible | dominant phenotype, suppressible by GstE12^EP2452/Scer\GAL4^ey.PH

(Singh et al., 2005)

L² has visible | dominant phenotype, suppressible by CG42709^EP3622/Scer\GAL4^ey.PH

(Singh et al., 2005)

L² has visible | dominant phenotype, suppressible by CG3308^EP1230/Scer\GAL4^ey.PH

(Singh et al., 2005)

L² has visible | dominant phenotype, suppressible by Scer\GAL4^ey.PH/Hmt-1^EP3387

(Singh et al., 2005)

L² has visible | dominant phenotype, suppressible by Scer\GAL4^ey.PH/CG7920^EP3096

(Singh et al., 2005)

L² has visible | dominant phenotype, suppressible by Df(1)Bar/+

(Singh et al., 2005)

L² has visible | dominant phenotype, suppressible by Df(3L)iro-DFM3

(Singh et al., 2005)

L² has visible | dominant phenotype, suppressible by mir-278^EP1229/Scer\GAL4^ey.PH

(Singh et al., 2005)

L² has visible | dominant phenotype, suppressible by Scer\GAL4^ey.PH/EP1324^EP1324

(Singh et al., 2005)

L² has visible | dominant phenotype, suppressible by EP2012^EP2012/Scer\GAL4^ey.PH

(Singh et al., 2005)

L² has visible | dominant phenotype, suppressible by EP2031^EP2031/Scer\GAL4^ey.PH

(Singh et al., 2005)

L² has visible | dominant phenotype, suppressible by Scer\GAL4^ey.PH/EP2370^EP2370

(Singh et al., 2005)

L² has visible | dominant phenotype, suppressible by EP3123^EP3123/Scer\GAL4^ey.PH

(Singh et al., 2005)

L² has visible | dominant phenotype, suppressible by NFAT^EP1353/Scer\GAL4^ey.PH

(Singh et al., 2005)

L² has visible | dominant phenotype, suppressible by NFAT^EP1508/Scer\GAL4^ey.PH

(Singh et al., 2005)

L² has visible | dominant phenotype, suppressible by RhoGAP68F^EP3152/Scer\GAL4^ey.PH

(Singh et al., 2005)

L² has visible | dominant phenotype, suppressible by TfIIA-S^EP3285/Scer\GAL4^ey.PH

(Singh et al., 2005)

L² has visible | dominant phenotype, suppressible by Cul4^EP2518/Scer\GAL4^ey.PH

(Singh et al., 2005)

L² has visible | dominant phenotype, suppressible by dco^EP3280/Scer\GAL4^ey.PH

(Singh et al., 2005)

L² has visible | dominant phenotype, suppressible by emc[+]/emc¹²

(Singh et al., 2005)

L² has visible | dominant phenotype, suppressible by esg^EP2159/Scer\GAL4^ey.PH

(Singh et al., 2005)

L² has visible | dominant phenotype, suppressible by esg^EP2408/Scer\GAL4^ey.PH

(Singh et al., 2005)

L² has visible | dominant phenotype, suppressible by esg^EP633/Scer\GAL4^ey.PH

(Singh et al., 2005)

L² has visible | dominant phenotype, suppressible by esg^EP683/Scer\GAL4^ey.PH

(Singh et al., 2005)

L² has visible | dominant phenotype, suppressible by fng^EP3082/Scer\GAL4^ey.PH

(Singh et al., 2005)

L² has visible | dominant phenotype, suppressible by hh^EP3521/Scer\GAL4^ey.PH

(Singh et al., 2005)

L² has visible | dominant phenotype, suppressible by Scer\GAL4^ey.PH/kek1^EP655

(Singh et al., 2005)

L² has visible | dominant phenotype, suppressible by noc^EP2173/Scer\GAL4^ey.PH

(Singh et al., 2005)

L² has visible | dominant phenotype, suppressible by nrv1^EP2454/Scer\GAL4^ey.PH

(Singh et al., 2005)

L² has visible | dominant phenotype, suppressible by pnr[+]/pnr^VX6

(Singh et al., 2005)

L² has visible | dominant phenotype, suppressible by ras^EP1235/Scer\GAL4^ey.PH

(Singh et al., 2005)

L² has visible | dominant phenotype, suppressible by ras^EP1427/Scer\GAL4^ey.PH

(Singh et al., 2005)

L² has visible | dominant phenotype, suppressible by smo^EP1220/Scer\GAL4^ey.PH

(Singh et al., 2005)

L² has visible | dominant phenotype, suppressible by wun^EP2208/Scer\GAL4^ey.PH

(Singh et al., 2005)

L² has visible | dominant phenotype, suppressible by sgg^EP1576/Scer\GAL4^ey.PH

(Singh et al., 2005)

NOT suppressed by

Statement

Reference

L² has visible | dominant phenotype, non-suppressible by PRAS40[+]/PRAS40^P17

(Son and Choi, 2020)

L² has visible | dominant phenotype, non-suppressible by PRAS40[+]/PRAS40^KO

(Son and Choi, 2020)

L² has visible | dominant phenotype, non-suppressible by PRAS40^RNAi.WIZ.UAS/Scer\GAL4^ey.PU

(Son and Choi, 2020)

L² has visible | dominant phenotype, non-suppressible by phyl^JF03369/Scer\GAL4^ey.PU

(Son and Choi, 2020)

L² has visible | dominant phenotype, non-suppressible by phyl^GD12579/Scer\GAL4^ey.PU

(Son and Choi, 2020)

L² has visible | dominant phenotype, non-suppressible by Scer\GAL4^ey.PU/Achl^JF01976

(Son and Choi, 2020)

L² has increased cell death | dominant | larval stage phenotype, non-suppressible by p53^259H.GUS/Scer\GAL4^ey.PH

(Singh et al., 2006)

Enhancer of

Statement

Reference

L²/L[+] is an enhancer of visible phenotype of Scer\GAL4^ey.PH, pnr^D4.UAS

(Singh et al., 2005)

L²/L[+] is an enhancer of visible phenotype of Scer\GAL4^bi-omb-Gal4, pnr^D4.UAS

(Singh et al., 2005)

Other

Statement

Reference

EP1350^EP1350, L²/L[+], Scer\GAL4^ey.PH has lethal | pupal stage phenotype

(Singh et al., 2005)

L², Scer\GAL4^ey.PH, rod^EP3408 has lethal | dominant | pupal stage phenotype

(Singh et al., 2005)

L²/L[+], Scer\GAL4^ey.PH, pnr^D4.UAS has lethal | pupal stage phenotype

(Singh et al., 2005)

L²/L[+], Scer\GAL4^ey.PH, ush^UAS.cFa has lethal phenotype

(Singh et al., 2005)

Dad^EP3196, L², Scer\GAL4^ey.PH has lethal | dominant | pupal stage phenotype

(Singh et al., 2005)

L², Scer\GAL4^ey.PH, aop^EP2500 has lethal | dominant | pupal stage phenotype

(Singh et al., 2005)

L², Scer\GAL4^ey.PH, aop^EP598 has lethal | dominant | pupal stage phenotype

(Singh et al., 2005)

EP1350^EP1350, L², Scer\GAL4^ey.PH has lethal | pupal stage phenotype

(Singh et al., 2005)

L², Scer\GAL4^ey.PH, ush^UAS.cFa has lethal phenotype

(Singh et al., 2005)

L², Scer\GAL4^ey.PH, pnr^D4.UAS has lethal | pupal stage phenotype

(Singh et al., 2005)

L²/L[+], RpL27A¹ has lethal | dominant phenotype

(Dunn and Mossige, 1937)

L², RpL27A¹/M(2)24F[+] has lethal | dominant phenotype

(Dunn and Mossige, 1937)

Phenotype Manifest In

Enhanced by

Statement

Reference

L² has eye | somatic clone phenotype, enhanceable by hth[+]/hth^100-1

(Singh et al., 2011)

L² has eye disc | somatic clone phenotype, enhanceable by hth[+]/hth^100-1

(Singh et al., 2011)

L² has eye | somatic clone phenotype, enhanceable by hth[+]/hth^Meis1-P2

(Singh et al., 2011)

L² has eye disc | somatic clone phenotype, enhanceable by hth[+]/hth^Meis1-P2

(Singh et al., 2011)

L² has eye | somatic clone phenotype, enhanceable by hth^UAS.cPa/Scer\GAL4^ey.PH

(Singh et al., 2011)

L² has eye disc | somatic clone phenotype, enhanceable by hth^UAS.cPa/Scer\GAL4^ey.PH

(Singh et al., 2011)

L² has eye disc | ventral phenotype, enhanceable by Scer\GAL4^ey.PH/wg^UAS.cAa

(Singh et al., 2006)

L² has eye | ventral phenotype, enhanceable by Scer\GAL4^ey.PH/wg^UAS.cAa

(Singh et al., 2006)

L² has eye disc | ventral phenotype, enhanceable by dsh^UAS.Tag:MYC/Scer\GAL4^ey.PH

(Singh et al., 2006)

L² has eye | ventral phenotype, enhanceable by dsh^UAS.Tag:MYC/Scer\GAL4^ey.PH

(Singh et al., 2006)

L² has eye disc | ventral phenotype, enhanceable by bsk^UAS.cBa/Scer\GAL4^ey.PH

(Singh et al., 2006)

L² has eye | ventral phenotype, enhanceable by bsk^UAS.cBa/Scer\GAL4^ey.PH

(Singh et al., 2006)

L² has eye disc | ventral phenotype, enhanceable by Jra^Asp.B.UAS/Scer\GAL4^ey.PH

(Singh et al., 2006)

L² has eye | ventral phenotype, enhanceable by Jra^Asp.B.UAS/Scer\GAL4^ey.PH

(Singh et al., 2006)

L² has eye disc | ventral phenotype, enhanceable by arm^UAS.cUa/Scer\GAL4^ey.PH

(Singh et al., 2006)

L² has eye | ventral phenotype, enhanceable by arm^UAS.cUa/Scer\GAL4^ey.PH

(Singh et al., 2006)

L² has eye phenotype, enhanceable by rod^EP3408/Scer\GAL4^ey.PH

(Singh et al., 2005)

L² has eye phenotype, enhanceable by +/Df(2L)BSC7

(Singh et al., 2005)

L² has eye phenotype, enhanceable by Scer\GAL4^ey.PH/EP1350^EP1350

(Singh et al., 2005)

L² has eye phenotype, enhanceable by Scer\GAL4^ey.PH/EP1410^EP1410

(Singh et al., 2005)

L² has eye phenotype, enhanceable by EP3087^EP3087/Scer\GAL4^ey.PH

(Singh et al., 2005)

L² has eye phenotype, enhanceable by EP950^EP950/Scer\GAL4^ey.PH

(Singh et al., 2005)

L² has eye phenotype, enhanceable by ara^UAS.cGa/Scer\GAL4^ey.PH

(Singh et al., 2005)

L² has eye phenotype, enhanceable by Scer\GAL4^ey.PH/dally^EP3168

(Singh et al., 2005)

L² has eye phenotype, enhanceable by emc^EP3166/Scer\GAL4^ey.PH

(Singh et al., 2005)

L² has eye phenotype, enhanceable by emc^EP3614/Scer\GAL4^ey.PH

(Singh et al., 2005)

L² has eye phenotype, enhanceable by emc^EP3620/Scer\GAL4^ey.PH

(Singh et al., 2005)

L² has eye phenotype, enhanceable by Dad^EP3196/Scer\GAL4^ey.PH

(Singh et al., 2005)

L² has eye phenotype, enhanceable by emc^EP415/Scer\GAL4^ey.PH

(Singh et al., 2005)

L² has eye phenotype, enhanceable by esg⁰⁵⁷²⁹/esg[+]

(Singh et al., 2005)

L² has eye phenotype, enhanceable by hh^bar3/hh^bar3

(Singh et al., 2005)

L² has eye phenotype, enhanceable by Scer\GAL4^ey.PH/mirr^UAS.cMa

(Singh et al., 2005)

L² has eye phenotype, enhanceable by nej^EP1149/Scer\GAL4^ey.PH

(Singh et al., 2005)

L² has eye phenotype, enhanceable by Scer\GAL4^ey.PH/nej^EP1179

(Singh et al., 2005)

L² has eye phenotype, enhanceable by sgg[+]/sgg¹

(Singh et al., 2005)

L² has eye phenotype, enhanceable by aop^EP2500/Scer\GAL4^ey.PH

(Singh et al., 2005)

L² has eye phenotype, enhanceable by aop^EP598/Scer\GAL4^ey.PH

(Singh et al., 2005)

L² has eye phenotype, enhanceable by B[+]/Bar¹

(Singh et al., 2005)

NOT Enhanced by

Statement

Reference

L² has eye phenotype, non-enhanceable by PRAS40[+]/PRAS40^P17

(Son and Choi, 2020)

L² has eye phenotype, non-enhanceable by PRAS40[+]/PRAS40^KO

(Son and Choi, 2020)

L² has eye phenotype, non-enhanceable by PRAS40^RNAi.WIZ.UAS/Scer\GAL4^ey.PU

(Son and Choi, 2020)

L² has eye phenotype, non-enhanceable by phyl^JF03369/Scer\GAL4^ey.PU

(Son and Choi, 2020)

L² has eye phenotype, non-enhanceable by phyl^GD12579/Scer\GAL4^ey.PU

(Son and Choi, 2020)

L² has eye phenotype, non-enhanceable by Scer\GAL4^ey.PU/Achl^JF01976

(Son and Choi, 2020)

L² has eye disc | somatic clone phenotype, non-enhanceable by exd[+]/exd¹

(Singh et al., 2011)

L² has eye | somatic clone phenotype, non-enhanceable by exd[+]/exd¹

(Singh et al., 2011)

L² has eye disc | somatic clone phenotype, non-enhanceable by exd^UAS.cGa/Scer\GAL4^ey.PH

(Singh et al., 2011)

L² has eye | somatic clone phenotype, non-enhanceable by exd^UAS.cGa/Scer\GAL4^ey.PH

(Singh et al., 2011)

L² has eye disc | ventral phenotype, non-enhanceable by p53^UAS.Ex/Scer\GAL4^ey.PH

(Singh et al., 2006)

L² has eye | ventral phenotype, non-enhanceable by p53^UAS.Ex/Scer\GAL4^ey.PH

(Singh et al., 2006)

Suppressed by

Statement

Reference

L² has eye | somatic clone phenotype, suppressible by hth^dtl-S142204/hth[+]

(Singh et al., 2011)

L² has eye disc | somatic clone phenotype, suppressible by hth^dtl-S142204/hth[+]

(Singh et al., 2011)

L² has eye phenotype, suppressible by hth^{1-430.EnR.UAS}/Scer\GAL4^ey.PH

(Singh et al., 2011)

L² has eye disc phenotype, suppressible by hth^{1-430.EnR.UAS}/Scer\GAL4^ey.PH

(Singh et al., 2011)

L² has eye phenotype, suppressible by hyx^EY06898

(Zhang et al., 2010)

L² has eye | ventral phenotype, suppressible by Diap1^UAS.cLa/Scer\GAL4^ey.PH

(Singh et al., 2006)

L² has eye disc | ventral phenotype, suppressible by Df(3L)H99/+

(Singh et al., 2006)

L² has eye | ventral phenotype, suppressible by Df(3L)H99/+

(Singh et al., 2006)

L² has eye | ventral phenotype, suppressible by Scer\GAL4^ey.PH/sgg^S9A.UAS

(Singh et al., 2006)

L² has eye disc | ventral phenotype, suppressible by Scer\GAL4^ey.PH/sgg^S9A.UAS

(Singh et al., 2006)

L² has eye | ventral phenotype, suppressible by pan^ΔN.UAS/Scer\GAL4^ey.PH

(Singh et al., 2006)

L² has eye disc | ventral phenotype, suppressible by pan^ΔN.UAS/Scer\GAL4^ey.PH

(Singh et al., 2006)

L² has eye | ventral phenotype, suppressible by wg¹

(Singh et al., 2006)

L² has eye disc | ventral phenotype, suppressible by wg¹

(Singh et al., 2006)

L² has eye disc | ventral | second instar larval stage phenotype, suppressible by wg^l-12

(Singh et al., 2006)

L² has eye disc | ventral phenotype, suppressible by puc^UAS.cMa/Scer\GAL4^ey.PH

(Singh et al., 2006)

L² has eye | ventral phenotype, suppressible by puc^UAS.cMa/Scer\GAL4^ey.PH

(Singh et al., 2006)

L² has eye disc | ventral phenotype, suppressible by bsk^DN.UAS/Scer\GAL4^ey.PH

(Singh et al., 2006)

L² has eye | ventral phenotype, suppressible by bsk^DN.UAS/Scer\GAL4^ey.PH

(Singh et al., 2006)

L² has eye disc | ventral phenotype, suppressible by hep[+]/hep^r75

(Singh et al., 2006)

L² has eye | ventral phenotype, suppressible by hep[+]/hep^r75

(Singh et al., 2006)

L² has eye disc | ventral phenotype, suppressible by Scer\GAL4^ey.PH/BacA\p35^UAS.cHa

(Singh et al., 2006)

L² has eye | ventral phenotype, suppressible by Scer\GAL4^ey.PH/BacA\p35^UAS.cHa

(Singh et al., 2006)

L² has eye | ventral phenotype, suppressible by puc^UAS.cMa/Scer\GAL4^ey.PH/BacA\p35^UAS.cHa

(Singh et al., 2006)

L² has eye disc | ventral phenotype, suppressible by Diap1^UAS.cLa/Scer\GAL4^ey.PH

(Singh et al., 2006)

L² has eye | ventral phenotype, suppressible by pygo^unspecified

(Singh et al., 2005)

L² has eye | ventral phenotype, suppressible by ush^UAS.cFa/Scer\GAL4^ey.PH

(Singh et al., 2005)

L² has eye | ventral phenotype, suppressible by CBP^EP1643/Scer\GAL4^ey.PH

(Singh et al., 2005)

L² has eye | ventral phenotype, suppressible by CG12054^EP3309/Scer\GAL4^ey.PH

(Singh et al., 2005)

L² has eye | ventral phenotype, suppressible by GstE12^EP2452/Scer\GAL4^ey.PH

(Singh et al., 2005)

L² has eye | ventral phenotype, suppressible by CG42709^EP3622/Scer\GAL4^ey.PH

(Singh et al., 2005)

L² has eye | ventral phenotype, suppressible by CG3308^EP1230/Scer\GAL4^ey.PH

(Singh et al., 2005)

L² has eye | ventral phenotype, suppressible by Scer\GAL4^ey.PH/Hmt-1^EP3387

(Singh et al., 2005)

L² has eye | ventral phenotype, suppressible by Scer\GAL4^ey.PH/CG7920^EP3096

(Singh et al., 2005)

L² has eye | ventral phenotype, suppressible by Df(1)Bar/+

(Singh et al., 2005)

L² has eye | ventral phenotype, suppressible by Df(3L)iro-DFM3

(Singh et al., 2005)

L² has eye | ventral phenotype, suppressible by mir-278^EP1229/Scer\GAL4^ey.PH

(Singh et al., 2005)

L² has eye | ventral phenotype, suppressible by Scer\GAL4^ey.PH/EP1324^EP1324

(Singh et al., 2005)

L² has eye | ventral phenotype, suppressible by EP2012^EP2012/Scer\GAL4^ey.PH

(Singh et al., 2005)

L² has eye | ventral phenotype, suppressible by EP2031^EP2031/Scer\GAL4^ey.PH

(Singh et al., 2005)

L² has eye | ventral phenotype, suppressible by Scer\GAL4^ey.PH/EP2370^EP2370

(Singh et al., 2005)

L² has eye | ventral phenotype, suppressible by EP3123^EP3123/Scer\GAL4^ey.PH

(Singh et al., 2005)

L² has eye | ventral phenotype, suppressible by NFAT^EP1353/Scer\GAL4^ey.PH

(Singh et al., 2005)

L² has eye | ventral phenotype, suppressible by NFAT^EP1508/Scer\GAL4^ey.PH

(Singh et al., 2005)

L² has eye | ventral phenotype, suppressible by RhoGAP68F^EP3152/Scer\GAL4^ey.PH

(Singh et al., 2005)

L² has eye | ventral phenotype, suppressible by TfIIA-S^EP3285/Scer\GAL4^ey.PH

(Singh et al., 2005)

L² has eye | ventral phenotype, suppressible by Cul4^EP2518/Scer\GAL4^ey.PH

(Singh et al., 2005)

L² has eye | ventral phenotype, suppressible by dco^EP3280/Scer\GAL4^ey.PH

(Singh et al., 2005)

L² has eye | ventral phenotype, suppressible by emc[+]/emc¹²

(Singh et al., 2005)

L² has eye | ventral phenotype, suppressible by esg^EP2159/Scer\GAL4^ey.PH

(Singh et al., 2005)

L² has eye | ventral phenotype, suppressible by esg^EP2408/Scer\GAL4^ey.PH

(Singh et al., 2005)

L² has eye | ventral phenotype, suppressible by esg^EP633/Scer\GAL4^ey.PH

(Singh et al., 2005)

L² has eye | ventral phenotype, suppressible by esg^EP683/Scer\GAL4^ey.PH

(Singh et al., 2005)

L² has eye | ventral phenotype, suppressible by fng^EP3082/Scer\GAL4^ey.PH

(Singh et al., 2005)

L² has eye | ventral phenotype, suppressible by hh^EP3521/Scer\GAL4^ey.PH

(Singh et al., 2005)

L² has eye | ventral phenotype, suppressible by Scer\GAL4^ey.PH/kek1^EP655

(Singh et al., 2005)

L² has eye | ventral phenotype, suppressible by noc^EP2173/Scer\GAL4^ey.PH

(Singh et al., 2005)

L² has eye | ventral phenotype, suppressible by nrv1^EP2454/Scer\GAL4^ey.PH

(Singh et al., 2005)

L² has eye | ventral phenotype, suppressible by pnr[+]/pnr^VX6

(Singh et al., 2005)

L² has eye | ventral phenotype, suppressible by ras^EP1235/Scer\GAL4^ey.PH

(Singh et al., 2005)

L² has eye | ventral phenotype, suppressible by ras^EP1427/Scer\GAL4^ey.PH

(Singh et al., 2005)

L² has eye | ventral phenotype, suppressible by sgg^EP1576/Scer\GAL4^ey.PH

(Singh et al., 2005)

L² has eye | ventral phenotype, suppressible by smo^EP1220/Scer\GAL4^ey.PH

(Singh et al., 2005)

L² has eye | ventral phenotype, suppressible by wun^EP2208/Scer\GAL4^ey.PH

(Singh et al., 2005)

NOT suppressed by

Statement

Reference

L² has eye phenotype, non-suppressible by PRAS40[+]/PRAS40^KO

(Son and Choi, 2020)

L² has eye phenotype, non-suppressible by PRAS40^RNAi.WIZ.UAS/Scer\GAL4^ey.PU

(Son and Choi, 2020)

L² has eye phenotype, non-suppressible by phyl^JF03369/Scer\GAL4^ey.PU

(Son and Choi, 2020)

L² has eye phenotype, non-suppressible by phyl^GD12579/Scer\GAL4^ey.PU

(Son and Choi, 2020)

L² has eye phenotype, non-suppressible by Scer\GAL4^ey.PU/Achl^JF01976

(Son and Choi, 2020)

L² has eye phenotype, non-suppressible by PRAS40[+]/PRAS40^P17

(Son and Choi, 2020)

L² has eye disc | somatic clone phenotype, non-suppressible by exd[+]/exd¹

(Singh et al., 2011)

L² has eye | somatic clone phenotype, non-suppressible by exd[+]/exd¹

(Singh et al., 2011)

L² has eye disc | somatic clone phenotype, non-suppressible by exd^UAS.cGa/Scer\GAL4^ey.PH

(Singh et al., 2011)

L² has eye | somatic clone phenotype, non-suppressible by exd^UAS.cGa/Scer\GAL4^ey.PH

(Singh et al., 2011)

L² has eye disc | ventral phenotype, non-suppressible by p53^259H.GUS/Scer\GAL4^ey.PH

(Singh et al., 2006)

L² has eye | ventral phenotype, non-suppressible by p53^259H.GUS/Scer\GAL4^ey.PH

(Singh et al., 2006)

L² has eye disc | ventral | first instar larval stage phenotype, non-suppressible by wg^l-12

(Singh et al., 2006)

L² has eye disc | ventral | third instar larval stage phenotype, non-suppressible by wg^l-12

(Singh et al., 2006)

Enhancer of

Statement

Reference

L²/L[+] is an enhancer of eye phenotype of Scer\GAL4^ey.PH, wg^UAS.cAa

(Singh et al., 2006)

L²/L[+] is an enhancer of eye disc phenotype of Scer\GAL4^ey.PH, wg^UAS.cAa

(Singh et al., 2006)

L²/L[+] is an enhancer of eye phenotype of bsk^UAS.cBa

(Singh et al., 2006)

L²/L[+] is an enhancer of eye phenotype of Scer\GAL4^ey.PH, arm^UAS.cUa

(Singh et al., 2006)

L²/L[+] is an enhancer of eye disc phenotype of Scer\GAL4^ey.PH, arm^UAS.cUa

(Singh et al., 2006)

L²/L[+] is an enhancer of eye phenotype of Scer\GAL4^ey.PH, pnr^D4.UAS

(Singh et al., 2005)

L²/L[+] is an enhancer of eye phenotype of Scer\GAL4^bi-omb-Gal4, pnr^D4.UAS

(Singh et al., 2005)

Other

Statement

Reference

L²/L[+], Scer\GAL4^ey.PH, pnr^D4.UAS has adult head phenotype

(Singh et al., 2005)

L², Scer\GAL4^ey.PH, pnr^D4.UAS has adult head phenotype

(Singh et al., 2005)

Additional Comments

Genetic Interactions

Statement

Reference

Increasing levels of hth in L² heterozygotes through expression of hth^Scer\UAS.cPa under the control of Scer\GAL4^ey.PH enhances the ventral eye loss to a 'no eye' phenotype. This loss of eye fate is due to an eye-to-cuticle fate change. There is no effect on the antennal field.

A hth^dtl-S142204 heterozygous background partially suppresses the loss-of-ventral eye phenotype found in L²/+ mutants.

Loss-of-function L² heterozygous eye clones in a hth^100-1 or hth^Meis1-P2 heterozygous background results in a complete loss-of-ventral eye phenotype, as seen in L² eye clones.

exd¹/+; L²/+ double heterozygous mutants exhibit the same loss-of-ventral-eye phenotype as L²/+ mutants.

Over-expression of exd^Scer\UAS.cGa under the control of Scer\GAL4^ey.PH in a L² heterozygous background does not affect the loss-of-ventral-eye phenotype found in L²/+ mutants.

(Singh et al., 2011)

Expression of p53^Scer\UAS.Ex under the control of Scer\GAL4^ey.PH does not enhance the L²/+ mutant phenotype of ventral eye loss. Further, expression of the dominant negative p53^259H.GUS transgene, under the control of Scer\GAL4^ey.PH, does not suppress the L²/+ phenotype.

Expression of Diap1^Scer\UAS.cLa under the control of Scer\GAL4^ey.PH results in a partial suppression of the L²/+ phenotype. 27% of rescued flies have eyes that are more than three-quarters of the wild-type size and a range of intermediate and weaker rescues are observable in the other 73%.

L²/+; Df(3L)H99/+ flies show an almost complete rescue of the L²/+ phenotype in 38% of adult flies.

The L²/+ loss-of-ventral eye phenotype can be rescued by reducing canonical wg signaling components. The ventral eye is restored in L², wg¹/+ double mutants, in which ommatidia in the restored ventral section show correct ventral eye polarity. The temperature sensitive wg^l-12 allele can only significantly rescue the L² ventral eye loss phenotype when larvae are shifted to the restrictive temperature during the second larval instar; shifting to the restrictive temperature during the first or third instar has no effect on the L² phenotype. Expression of sgg^S9A.Scer\UAS under the control of Scer\GAL4^ey.PH provides a partial rescue of the ventral eye loss phenotype of L²/+ flies; the suppression is significant in 26% of flies and is weak in the remaining flies, while expression of pan^ΔN.Scer\UAS under the control of Scer\GAL4^ey.PH significantly rescues the ventral eye loss of L²/+ flies in 23% of cases.

Increasing wg signaling leads to an enhancement of the L²/+ loss-of-ventral eye phenotype. Expression of either wg^Scer\UAS.cAa or arm^Scer\UAS.cUa, under the control of Scer\GAL4^ey.PH, in L²/+ mutants results in a no eye phenotype, while small eyes are observed in all of the single mutants. Expression of dsh^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^ey.PH leads to an enhancement of the ventral eye loss.

Blocking JNK signaling significantly represses the L²/+ loss-of-ventral eye phenotype. Expression of puc^Scer\UAS.cMa (a JNK down-regulator) under the control of Scer\GAL4^ey.PH results in rescue of the ventral eye loss of L²/+ mutants in 36% of cases. Expression of the dominant negative bsk^DN.Scer\UAS under the control of Scer\GAL4^ey.PH results in rescue of the ventral eye loss of L²/+ mutants in 36% of cases, while 32% of hep^r75/+; L²/+ flies show a strong suppression of the ventral eye loss phenotype seen in L²/+ flies. In contrast, increasing JNK signaling enhances the phenotype; expression of bsk^Scer\UAS.cBa under the control of Scer\GAL4^ey.PH enhances the loss-of-ventral-eye phenotype to near complete loss in 39% of L²/+ flies, while expression of Jra^{Asp.B.Scer\UAS} under the control of Scer\GAL4^ey.PH results in a strong enhancement of the L²/+ eye phenotype to a very small eye or no eye in 36% of flies.

*j Free text: Expression of BacA\p35^Scer\UAS.cHa under the control of Scer\GAL4^ey.PH results in a partial suppression of the ventral eye loss in L²/+ flies; 40% of rescued flies have ventral eyes that are more than three-quarters of the wild-type size. The remaining 60% of rescued flies show a range of weaker phenotypes than L²/+ eyes.

Coexpression of BacA\p35^Scer\UAS.cHa and puc^Scer\UAS.cMa under the control of Scer\GAL4^ey.PH rescues the ventral eye loss of L²/+ flies to a near wild-type eye in 69% of flies and pharate adults; this is a much greater suppression than that seen when either transgene is expressed without the other.

(Singh et al., 2006)

sgg¹/+ ; L²/+ flies show complete elimination of the eye field in 15% of cases. L²/+ ; hh^bar3/hh^bar3 and L²/Df(2L)BSC7 flies show complete elimination of the eye. L²/+ pharate adults expressing EP1350^EP1350 under the control of Scer\GAL4^ey.PH have a no eye phenotype, but the antennal field is not affected. B¹/+ ; L²/+ animals have a no eye phenotype. Only 41% of L²/+ animals expressing pnr^D4.Scer\UAS under the control of Scer\GAL4^ey.PH eclose, and these animals have greatly reduced heads with no eyes, but normal antennae. Only 41% of L²/+ animals expressing pnr^D4.Scer\UAS under the control of Scer\GAL4^ey.PH eclose, and these animals have greatly reduced heads with no eyes, but normal antennae. L²/+ increases the frequency of the no-eye phenotype seen in discs of animals expressing ush^Scer\UAS.cFa under the control of Scer\GAL4^ey.PH at 29^oC throughout development from 80% to nearly 98% and the animals fail to eclose. The L²/+ phenotype of ventral eye loss is unaffected if expression of ush^Scer\UAS.cFa is driven by Scer\GAL4^ey.PH at 29^oC from embryogenesis until the end of the first larval instar, or by Scer\GAL4^ey.PH at 29^oC from during the third larval instar. However, if ush^Scer\UAS.cFa expression is driven by Scer\GAL4^ey.PH at 29^oC during the second larval instar, the L²/+ ventral eye phenotype is significantly rescued.

(Singh et al., 2005)

Xenogenetic Interactions

Statement

Reference

Misexpression of en::hth^{R.1-430.Scer\UAS} in L² under the control of Scer\GAL4^ey.PH mutants causes a significant suppression of the loss-of-ventral eye phenotype in the larval eye imaginal disc and the adult eye.

(Singh et al., 2011)

Expression of BacA\p35^Scer\UAS.cHa under the control of Scer\GAL4^ey.PH results in a partial suppression of the ventral eye loss in L²/+ flies; 40% of rescued flies have ventral eyes that are more than three-quarters of the wild-type size. The remaining 60% of rescued flies show a range of weaker phenotypes than L²/+ eyes.

(Singh et al., 2006)

Complementation and Rescue Data

Rescued by

L²/L[+] is rescued by L^GLV21080/Scer\GAL4^ey.PU

(Son and Choi, 2020)

Partially rescued by

L²/L[+] is partially rescued by L^JF01943/Scer\GAL4^ey.PU

(Son and Choi, 2020)

L²/L[+] is partially rescued by Scer\GAL4^ey.PU/L^KK102438

(Son and Choi, 2020)

Comments

Expression of either L^KK102438 or L^JF01943 under the control of Scer\GAL4^ey.PU partially suppresses the L²/+ eye phenotype.

(Son and Choi, 2020)

Images (0)

Mutant

Wild-type

Stocks (24)

Bloomington

319

L²

6056

Df(2L)64j, L²/CyO

1597

bic¹ L²/CyO

42695

y¹ w^*; L²/CyO; P{UAS-Dap160.K}4.1

6814

Cam⁵ L²/CyO, amos^Roi-1 bw¹

41744

w^* TI{TI}Ir8a¹; Bl¹ L²/CyO

6813

Cam^4c1 L² bw¹/CyO, P{sevRas1.V12}FK1

6811

Cam^3c1 L²/CyO, amos^Roi-1 bw¹

67172

y¹ w^*; L²/CyO; PBac{UAS-bsf.J}VK00033

Kyoto

105807

Bl¹ L²/SM5

106407

bic¹ L²/CyO

108570

Df(2L)64j, L²/CyO, Adh^nB

More stocks available...

Notes on Origin

Discoverer

Mohr, 2nd Feb. 1920.

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

L²

(Son and Chung, 2023, Mehta et al., 2021, Son and Choi, 2020, Avila et al., 2011, Singh et al., 2011, Zhang et al., 2010, Singh et al., 2006)

Name Synonyms

Secondary FlyBase IDs

References (16)

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