FlyBase Allele Report: Dmel\PRAS40[KO]

General Information

Symbol

Dmel\PRAS40^KO

Species

D. melanogaster

Name

FlyBase ID

FBal0298261

Feature type

allele

Associated gene

Dmel\PRAS40

Associated Insertion(s)

TI{TI}PRAS40^KO

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

amorphic allele - genetic evidence

Mutagen

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Pallares-Cartes et al., 2012)

amorphic allele - molecular evidence

(Pallares-Cartes et al., 2012)

Mutagen

Progenitor genotype

Associated Insertion(s)

TI{TI}PRAS40^KO

Cytology

Description

500bp of coding sequence from the second PRAS40 exon has been replaced with a mini-w marker, which causes the rest of the PRAS40 gene to be out of frame. Generated by homologous recombination using a donor construct based on the pRK1 plasmid.

(Pallares-Cartes et al., 2012)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

2R:14,495,151..14,495,667 [+]

Comment:

Inferred boundaries of a deletion mutation created by targeted homologous recombination.

(Pallares-Cartes et al., 2012)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

fertile

(Pallares-Cartes et al., 2012)

viable

(Pallares-Cartes et al., 2012)

Phenotype Manifest In

ovary

(Pallares-Cartes et al., 2012)

Detailed Description

Statement

Reference

Homozygous flies are viable and fertile and are not significantly delayed in development. They have no obvious eye patterning defects and are normal in weight. Total wing size and wing hair density are normal (indicating normal cell size).

Homozygous females have larger ovaries and lay more eggs per day than controls.

(Pallares-Cartes et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

PRAS40[+]/PRAS40^KO is a non-enhancer of visible | dominant phenotype of L²

(Son and Choi, 2020)

Suppressor of

Statement

Reference

PRAS40^KO is a suppressor of female sterile phenotype of chico¹

(Pallares-Cartes et al., 2012)

PRAS40^KO is a suppressor of male sterile phenotype of Pdk1⁵/Pdk1⁴

(Pallares-Cartes et al., 2012)

PRAS40^KO is a suppressor of female sterile phenotype of Pdk1⁵/Pdk1⁴

(Pallares-Cartes et al., 2012)

NOT Suppressor of

Statement

Reference

PRAS40[+]/PRAS40^KO is a non-suppressor of visible | dominant phenotype of L²

(Son and Choi, 2020)

PRAS40^KO is a non-suppressor of decreased body size phenotype of chico¹

(Pallares-Cartes et al., 2012)

PRAS40^KO is a non-suppressor of decreased cell size phenotype of chico¹

(Pallares-Cartes et al., 2012)

Other

Statement

Reference

PRAS40^KO, chico¹ has female fertile phenotype

(Pallares-Cartes et al., 2012)

PRAS40^KO, Tsc1^S533A.Tub, gig^4A.αTub has viable phenotype

(Pallares-Cartes et al., 2012)

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

PRAS40[+]/PRAS40^KO is a non-enhancer of eye phenotype of L²

(Son and Choi, 2020)

NOT Suppressor of

Statement

Reference

PRAS40[+]/PRAS40^KO is a non-suppressor of eye phenotype of L²

(Son and Choi, 2020)

Additional Comments

Genetic Interactions

Statement

Reference

PRAS40^KO, Tsc1^S533A.Tub, gig^4A.αTub flies are viable and normal in size.

PRAS40^KO completely rescues the sterility of chico¹ females, but does not suppress the reduced body and wing cell size of chico¹ flies.

The increased rate of egg laying seen in PRAS40^KO females is rescued by one copy of S6k⁰⁷⁰⁸⁴.

(Pallares-Cartes et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

PRAS40^KO is rescued by PRAS40^UAS.cCa/Scer\GAL4^hs.PU

(Pallares-Cartes et al., 2012)

PRAS40^KO is rescued by PRAS40^UAS.cCa/Scer\GAL4^nanos.PU

(Pallares-Cartes et al., 2012)

Comments

The increased rate of egg laying seen in PRAS40^KO females is rescued by expression of PRAS40^Scer\UAS.cCa under the control of either Scer\GAL4^hs.PU or Scer\GAL4^nos.PU.

(Pallares-Cartes et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

76339

w¹¹¹⁸; TI{TI}PRAS40^KO

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

PRAS40 KO

(Romero-Pozuelo et al., 2017)

PRAS40^KO

(Son and Choi, 2020, Pallares-Cartes et al., 2012)

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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