FlyBase Allele Report: Dmel\PRAS40[UAS.cCa]

General Information

Symbol

Dmel\PRAS40^UAS.cCa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Chern

FlyBase ID

FBal0144764

Feature type

allele

Associated gene

Dmel\PRAS40

Associated Insertion(s)

Carried in Construct

P{UAS-PRAS40.C}

Also Known As

UAS-L

Key Links

Transgenic product class

cDNA

(Chern and Choi, 2002)

wild_type

(Chern and Choi, 2002)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Chern and Choi, 2002)

wild_type

(Chern and Choi, 2002)

Mutagen

in vitro construct

(Chern and Choi, 2002)

Progenitor genotype

Carried in construct

P{UAS-PRAS40.C}

Cytology

Description

UASt regulatory sequences drive expression of a full length PRAS40 cDNA.

(Chern and Choi, 2002)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

PRAS40

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased cell size, with Scer\GAL4^en.PU

(Pallares-Cartes et al., 2012)

visible, with Scer\GAL4^en.PU

(Pallares-Cartes et al., 2012)

visible, with Scer\GAL4^ey.PH

(Singh and Choi, 2003)

visible, with Scer\GAL4^ey.PU

(Son and Choi, 2020)

Phenotype Manifest In

eye, with Scer\GAL4^ey.PH

(Singh and Choi, 2003)

eye, with Scer\GAL4^ey.PU

(Son and Choi, 2020)

eye disc, with Scer\GAL4^ey.PH

(Singh and Choi, 2003)

wing blade posterior compartment, with Scer\GAL4^en.PU

(Pallares-Cartes et al., 2012)

Detailed Description

Statement

Reference

Expression of PRAS40^UAS.cCa under the control of Scer\GAL4^ey.PU results in a small eye phenotype.

(Son and Choi, 2020)

Expression of PRAS40^Scer\UAS.cCa under the control of Scer\GAL4^en.PU results in a marked reduction in the size of the posterior compartment of the wing, which is associated with a reduction in cell size. No defects in wing vein or wing margin patterning are seen.

(Pallares-Cartes et al., 2012)

Expression of CG46146^Scer\UAS.cCa under the control of Scer\GAL4^bi-omb-Gal4 does not affect eye size.

(Singh et al., 2004)

Expression of CG46146^Scer\UAS.cCa under the control of Scer\GAL4^ey.PH results in selective elimination of the ventral eye in the disc and in the adult.

(Singh and Choi, 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

PRAS40^UAS.cCa, Scer\GAL4^en.PU has visible phenotype, suppressible | partially by Rheb^UAS.cUa, Scer\GAL4^en.PU

(Pallares-Cartes et al., 2012)

NOT suppressed by

Statement

Reference

PRAS40^UAS.cCa, Scer\GAL4^en.PU has visible phenotype, non-suppressible by BacA\p35^UAS.cUa, Scer\GAL4^en.PU

(Pallares-Cartes et al., 2012)

Suppressor of

Statement

Reference

PRAS40^UAS.cCa, Scer\GAL4^ey.PH is a suppressor | partially of visible phenotype of N^ECN.UAS, Scer\GAL4^ey.PH

(Chern and Choi, 2002)

NOT Suppressor of

Statement

Reference

PRAS40^UAS.cCa, Scer\GAL4^GMR.PF is a non-suppressor of visible phenotype of N^ECN.UAS, Scer\GAL4^GMR.PF

(Chern and Choi, 2002)

Other

Statement

Reference

PRAS40^UAS.cCa, Scer\GAL4^ey.PH, pnr^VX6 has visible | somatic clone phenotype

(Singh and Choi, 2003)

Phenotype Manifest In

Suppressed by

Statement

Reference

PRAS40^UAS.cCa, Scer\GAL4^en.PU has wing blade posterior compartment phenotype, suppressible | partially by Rheb^UAS.cUa, Scer\GAL4^en.PU

(Pallares-Cartes et al., 2012)

NOT suppressed by

Statement

Reference

PRAS40^UAS.cCa, Scer\GAL4^en.PU has wing blade posterior compartment phenotype, non-suppressible by BacA\p35^UAS.cUa, Scer\GAL4^en.PU

(Pallares-Cartes et al., 2012)

Suppressor of

Statement

Reference

PRAS40^UAS.cCa, Scer\GAL4^ey.PH is a suppressor | partially of eye | ventral phenotype of N^ECN.UAS, Scer\GAL4^ey.PH

(Chern and Choi, 2002)

NOT Suppressor of

Statement

Reference

PRAS40^UAS.cCa, Scer\GAL4^GMR.PF is a non-suppressor of eye phenotype of N^ECN.UAS, Scer\GAL4^GMR.PF

(Chern and Choi, 2002)

PRAS40^UAS.cCa, Scer\GAL4^GMR.PF is a non-suppressor of ommatidium phenotype of N^ECN.UAS, Scer\GAL4^GMR.PF

(Chern and Choi, 2002)

Other

Statement

Reference

PRAS40^UAS.cCa, REPTOR^UAS.cSa, Scer\GAL4^Mef2.PU has adult somatic muscle cell phenotype

(Saavedra et al., 2023)

PRAS40^UAS.cCa, REPTOR^UAS.cSa, Scer\GAL4^Mef2.PU has mitochondrion | adult stage phenotype

(Saavedra et al., 2023)

PRAS40^UAS.cCa, REPTOR^UAS.cSa, Scer\GAL4^Mef2.PU has dorsal longitudinal indirect flight muscle phenotype

(Saavedra et al., 2023)

PRAS40^UAS.cCa, Scer\GAL4^ey.PH, pnr^VX6 has eye | somatic clone phenotype

(Singh and Choi, 2003)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Rheb^Scer\UAS.cUa partially rescues the reduction in size of the posterior compartment of the wing which is caused by expression of PRAS40^Scer\UAS.cCa under the control of Scer\GAL4^en.PU.

(Pallares-Cartes et al., 2012)

Co-expression of tsh^Scer\UAS.cGa and CG46146^Scer\UAS.cCa under the control of Scer\GAL4^Act5C.PI in clones in the eye results in ventral eye suppression and dorsal eye enlargements.

(Singh et al., 2004)

Expression of CG46146^Scer\UAS.cCa under the control of Scer\GAL4^ey.PH in eye discs homozygous for pnr^VX6 (generated using the "EGUF" method to remove all eye disc cells except the homozygous pnr^VX6 clone cells) results in a small eye phenotype due to a reduction of the eye on both dorsal and ventral eye margins in nearly 20% of flies. This double mutant phenotype is different from either single mutant phenotype. The polarity of most of the ommatidia in the small eye is dorsal, along with a few ventral or with a polarity defect.

Expression of CG46146^Scer\UAS.cCa under the control of Scer\GAL4^ey.PH in eye discs homozygous for Df(3L)iro-DFM3 (generated using the "EGUF" method to remove all eye disc cells except the homozygous Df(3L)iro-DFM3 clone cells) results in a small eye phenotype due to a reduction of the eye on both dorsal and ventral eye margins in nearly 20% of flies.

(Singh and Choi, 2003)

The reduced eye phenotype caused by expression of N^ECN.Scer\UAS under the control of Scer\GAL4^ey.PH is partially suppressed in the ventral part of the eye by coexpression of CG46146^Scer\UAS.cCa. The rough eye phenotype caused by expression of N^ECN.Scer\UAS under the control of Scer\GAL4^GMR.PF is not suppressed by coexpression of CG46146^Scer\UAS.cCa.

(Chern and Choi, 2002)

Xenogenetic Interactions

Statement

Reference

Co-expression of BacA\p35^Scer\UAS.cUa fails to rescue the reduction in size of the posterior compartment of the wing which is caused by expression of PRAS40^Scer\UAS.cCa under the control of Scer\GAL4^en.PU.

(Pallares-Cartes et al., 2012)

Complementation and Rescue Data

Rescued by

PRAS40^UAS.cCa is rescued by PRAS40^GD6869/Scer\GAL4^ey.PU

(Son and Choi, 2020)

PRAS40^UAS.cCa is rescued by PRAS40^RNAi.WIZ.UAS/Scer\GAL4^ey.PU

(Son and Choi, 2020)

Rescues

PRAS40^UAS.cCa/Scer\GAL4^hs.PU rescues PRAS40^KO

(Pallares-Cartes et al., 2012)

PRAS40^UAS.cCa/Scer\GAL4^nanos.PU rescues PRAS40^KO

(Pallares-Cartes et al., 2012)

Comments

Co-expression of either PRAS40^GD6869 or PRAS40^{dsRNA.WIZ.UAS} fully rescues the small eye phenotype caused by expression of PRAS40^UAS.cCa under the control of Scer\GAL4^ey.PU.

PRAS40^P17/+ partially suppresses the small eye phenotype caused by expression of PRAS40^UAS.cCa under the control of Scer\GAL4^ey.PU.

(Son and Choi, 2020)

The increased rate of egg laying seen in PRAS40^KO females is rescued by expression of PRAS40^Scer\UAS.cCa under the control of either Scer\GAL4^hs.PU or Scer\GAL4^nos.PU.

(Pallares-Cartes et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

CG46146^Scer\UAS.cCa

L^Scer\UAS.cCa

PRAS40^Scer\UAS.cCa

PRAS40^UAS.cCa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Chern

(Chern and Choi, 2002)

Secondary FlyBase IDs

References (7)

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