FlyBase Allele Report: Dmel\hh[bar3]

General Information

Symbol

Dmel\hh^bar3

Species

D. melanogaster

Name

FlyBase ID

FBal0031487

Feature type

allele

Associated gene

Dmel\hh

Associated Insertion(s)

Carried in Construct

Also Known As

hh¹, bar-3

Key Links

Allele class

hypomorphic allele - genetic evidence

Mutagen

spontaneous

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Chotard et al., 2005, Epps et al., 1997, Lee et al., 1992)

Mutagen

spontaneous

Progenitor genotype

Cytology

Description

1885bp deletion within a hh intron. The coordinates of the hh deletion are bases 6053-7938 relative to the location of the P{PZ}hh^P30 insertion site.

(Rogers et al., 2005)

Deletion of non-coding sequences within the first intron.

(Chiang and Beachy, 1994)

1.8kb deletion of sequences between +6.0 and +7.8kb. 1.8kb deletion of sequences from position +6.0 to +7.8.

(Lee et al., 1992)

Associated with a deficiency of approximately 2kb.

(Mohler and Vani, 1992)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal planar polarity | recessive

(Chou and Chien, 2002)

abnormal size

(Wang et al., 2017)

abnormal size | adult stage (with hh²)

(Wang et al., 2017)

viable

(Rogers et al., 2005, Treisman et al., 1995, Lee et al., 1992)

visible (with hh²)

(Wang et al., 2017)

visible (with hh⁸)

(D'Costa et al., 2006, Jones et al., 2006)

visible | recessive

(Jones et al., 2006, Held, 1993, Lee et al., 1992, Mohler, 1988)

Phenotype Manifest In

crossvein | ectopic (with hh²)

(Wang et al., 2017)

embryonic/larval optic stalk

(Huang and Kunes, 1996)

eye

(Tokhunts et al., 2010, Jones et al., 2006, Rogers et al., 2005, Sedkov et al., 2003, Epps et al., 1997, Pan and Rubin, 1995, Chiang and Beachy, 1994)

eye (with hh⁸)

(D'Costa et al., 2006)

eye | anterior

(Pielage et al., 2004)

eye disc morphogenetic furrow

(Lim and Choi, 2004, Chou and Chien, 2002, Hummel et al., 2002, Huang and Kunes, 1996, Treisman and Rubin, 1995)

first posterior wing cell

(Palsson and Gibson, 2000)

glial cell

(Huang and Kunes, 1996)

lamina

(Huang and Kunes, 1996)

lamina & neuron

(Poeck et al., 2001)

lamina forming neuroblast

(Huang and Kunes, 1996)

lamina intrinsic neuron Lai

(Yoshida et al., 2005)

lamina tangential neuron

(Yoshida et al., 2005)

mesothoracic tarsal bristle longitudinal row 1

(Held, 1993)

mesothoracic tarsal bristle longitudinal row 2

(Held, 1993)

mesothoracic tarsal bristle longitudinal row 2.5

(Held, 1993)

mesothoracic tarsal bristle longitudinal row 3

(Held, 1993)

mesothoracic tarsal bristle longitudinal row 3.5

(Held, 1993)

mesothoracic tarsal bristle longitudinal row 4

(Held, 1993)

mesothoracic tarsal bristle longitudinal row 5

(Held, 1993)

mesothoracic tarsal bristle longitudinal row 5.5

(Held, 1993)

mesothoracic tarsal bristle longitudinal row 6

(Held, 1993)

mesothoracic tarsal bristle longitudinal row 6.5

(Held, 1993)

mesothoracic tarsal bristle longitudinal row 7

(Held, 1993)

mesothoracic tarsal bristle longitudinal row 8

(Held, 1993)

ommatidial precursor cluster

(Rogers et al., 2005)

ommatidial precursor cluster (with hh^AC)

(Rogers et al., 2005)

ommatidium

(Tokhunts et al., 2010, Jones et al., 2006, Chou and Chien, 2002, Epps et al., 1997, Levine et al., 1997)

ommatidium (with hh⁸)

(D'Costa et al., 2006, Jones et al., 2006)

photoreceptor cell & axon

(Huang and Kunes, 1996)

photoreceptor cell R7 & axon

(Hoyle et al., 2000)

photoreceptor cell R8 & axon

(Hoyle et al., 2000)

second posterior wing cell

(Palsson and Gibson, 2000)

wing

(Wang et al., 2017)

wing (with hh²)

(Wang et al., 2017)

Detailed Description

Statement

Reference

Most hh^bar3/hh^bar3 mutant adults exhibit a small reduction in the area between wing veins L3 and L4, and a small minority exhibit wing blisters, as compared to controls.

hh²/hh^bar3 mutant adults exhibit smaller wings with an ectopic crossvein, as compared to controls.

(Wang et al., 2017)

hh^bar3 mutant eye diss exhibit ommatidial developmental defects.

(Tokhunts et al., 2010)

The eyes of hh⁸/hh^bar3 flies are smaller than wild-type and are slightly rough, due to some disorganisation of ommatidial and inter-ommatidial structure.

(D'Costa et al., 2006)

Homozygotes have a rough eye which has an indented anterior side.

hh^bar3/hh⁸ flies have a slight rough eye phenotype.

(Jones et al., 2006)

hh^bar3 heterozygotes are not significantly different from wild-type.

hh^bar3 is a strong, eye-specific hypomorph. It is fully recessive in trans to wild-type, but has a severely reduced eye when homozygous (68% smaller than hh^bar3/+) and in trans to the null hh^AC it is smaller still (82% smaller than hh^bar3/+). This suggests that hh^bar3 is not an amorph for eye size by Muller's test: the phenotype becomes stronger in trans to the null.

hh^bar3 homozygotes have about 10 columns of ommatidia compared to 28 to 30 in wild-type.

hh^bar3/hh^AC mutants exhibit about six columns of ommatidia per eye compared to 28-30 columns in wild-type.

(Rogers et al., 2005)

R7 and R8 photoreceptor cell growth cones still form two layers in the medulla in hh^bar3 animals, although they remain closely associated during the early pupal stage (17% APF).

(Ting et al., 2005)

dac-positive lamina neurons fail to differentiate in hh^bar3 mutants.

(Yoshida et al., 2005)

The morphogenetic furrow becomes arrested in the eye discs of third instar mutant larvae.

(Lim and Choi, 2004)

The compound eyes of mutant animals lack anterior structures.

(Pielage et al., 2004)

Flies homozygous for hh^bar3 have narrow kidney-shaped eyes.

(Thomas and Ingham, 2003)

In hh^bar3 mutant eye discs, most of the ommatidial clusters over-rotate, with 33.7% of them reaching 110^o, compared to only 1.4% in wild-type discs (most ommatidial rotation in wild-type eye discs of late third larval and early pupal stages is about 50^o-60^o). The overrotation defect in these mutant discs correlated with the disappearance of the morphogenetic furrow (MF): In late third instar mutant eye discs the MF is still present, and most ommatidial clusters rotate to the normal position of 50^o-60^o, apart from a few in the most posterior region that over-rotate. As development progresses the MF becomes increasingly disrupted, and the over-rotation phenotype becomes more prominent. By the time MF progression is completely disrupted most of the ommatidial clusters have over-rotated, with many of them reaching 110^o-120^o. This overrotation phenotype persists to the adult stage, where 47.3% of ommatidia have a rotation degree larger than 105^o, compared to 1.1% in wild-type.

(Chou and Chien, 2002)

In hh^bar3 mutant larvae the posterior eye field develops normally, but anterior progression of the morphogenetic furrow is inhibited.

(Hummel et al., 2002)

A small number of lamina neurons develop compared to wild type in hh^bar3 animals. The development of glial cells is not affected. R1-R6 axons stop in the lamina (as in wild type) in these animals. The array of R7 and R8 growth cones in the medulla is indistinguishable from wild type.

(Poeck et al., 2001)

Defective spatial positioning of the R7,8 axons is seen in 18% of hh^bar3 homozygotes. Sometimes in just one lobe, and others in both. In the cases where both are affected the direction of the misorientation is the same.

(Hoyle et al., 2000)

Heterozygotes show a quantitative effect on wing shape in intervein regions C and D compared to wild type.

(Palsson and Gibson, 2000)

The eye is reduced in homozygotes, containing approximately 13 vertical rows of ommatidia from the posterior end.

(Epps et al., 1997)

Homozygotes have extremely reduced eyes, which contain approximately 150 (rather than the normal 800) ommatidia.

(Levine et al., 1997)

The eye discs contain about 11 columns of ommatidial R cell clusters. The most anterior clusters appear normal and the axon fascicles from them are found in their proper dorsoventral positions posterior to the lamina furrow. But viewed on their antero-posterior axis the fascicles are collapsed on each other. Glial cells are absent from the optic stalk and lamina. Lamina precursor cells fail to undergo their terminal division, arresting in G₁ at the lamina furrow.

(Huang and Kunes, 1996)

Homozygotes lack portions of the anterior eye, due to a defect in morphogenetic furrow progression. Eye phenotype is suppresses when in combination with Pka-C1^DN heterozygote, cell death is also suppressed.

(Pan and Rubin, 1995)

Arrests morphogenetic furrow movement after differentiation of 8-10 rows of photoreceptors. In wg^l-12; hh^bar3 eye discs the hh^bar3 mutation completely abolishes the effects of loss of wg expression (inwardly directed furrow movement is not present).

(Treisman and Rubin, 1995)

An eye specific hh allele. Double mutants of bun^rI043 and hh^bar3 all die as pupae.

(Treisman et al., 1995)

Adult eye is reduced in size. hh^bar3/hh⁶ produces an intermediate reduced eye phenotype.

(Chiang and Beachy, 1994)

When in combination with Df(2R)en^E/en¹ a phenotype reminiscent of a weak dpp mutation is produced, narrowing of the space between wing veins 3 and 4. Posterior wing clones affect adjacent cells of the anterior compartment and cause an overall reduction in wing size.

(Hidalgo, 1994)

The progression of the morphogenetic furrow in the developing eye disc arrests. The eye disc appears normal at the time of arrest. dpp expression is abolished (as assayed with a dpp-lacZ fusion gene).

(Heberlein et al., 1993)

Slight increase in bristles of all tarsal rows in the second leg.

(Held, 1993)

Transheterozygotes with hh^AC display an eye phenotype at 25^oC.

(Lee et al., 1992)

Adult viable allele.

(Mohler and Vani, 1992, Mohler, 1988)

A weak hypomorphic allele that is not complemented by other hh alleles. Eye of homozygote small and narrow with about 150 facets. Eye disc size reduced; deep cleft at anterior edge cell; clusters at cleft look mature (Renfranz and Benzer, 1989). viable

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

hh^bar3 has abnormal size phenotype, enhanceable by apt¹⁶⁷/apt[+]

(Wang et al., 2017)

hh^bar3/hh⁸ has visible phenotype, enhanceable by snama^PX1/mnm[+]

(Jones et al., 2006)

hh^bar3/hh⁸ has visible phenotype, enhanceable by l(2)rQ313[+]/snama^rQ313

(Jones et al., 2006)

hh^bar3/hh⁸ has visible phenotype, enhanceable by snama¹/mnm[+]

(Jones et al., 2006)

Suppressed by

Statement

Reference

hh^bar3/hh⁸ has visible phenotype, suppressible by BRWD3^ram1

(D'Costa et al., 2006)

hh^bar3 has abnormal planar polarity | recessive phenotype, suppressible by sca^UAS.cEa/Scer\GAL4^hs.2sev

(Chou and Chien, 2002)

NOT suppressed by

Statement

Reference

hh^bar3/hh⁸ has visible phenotype, non-suppressible by BRWD3^s5349/BRWD3[+]

(D'Costa et al., 2006)

Enhancer of

Statement

Reference

hh^bar3/hh^bar3 is an enhancer of visible | dominant phenotype of L²

(Singh et al., 2005)

hh[+]/hh^bar3 is an enhancer of abnormal planar polarity phenotype of sca^BP1/sca^BP2

(Chou and Chien, 2002)

NOT Suppressor of

Statement

Reference

hh[+]/hh^bar3 is a non-suppressor of abnormal size phenotype of L^fee

(Wang and Huang, 2009)

hh^bar3/hh^bar3 is a non-suppressor of abnormal planar polarity phenotype of nmo^j147-1/nmo^P1

(Chou and Chien, 2002)

Other

Statement

Reference

CycE², hh^bar3 has visible phenotype

(Wang et al., 2017)

CycE², hh^bar3 has abnormal size | adult stage phenotype

(Wang et al., 2017)

apt¹⁶⁷/apt[+], hh^bar3 has visible phenotype

(Wang et al., 2017)

Phenotype Manifest In

Enhanced by

Statement

Reference

hh^bar3 has wing phenotype, enhanceable by apt¹⁶⁷/apt[+]

(Wang et al., 2017)

hh^bar3/hh⁸ has eye phenotype, enhanceable by snama^PX1/mnm[+]

(Jones et al., 2006)

hh^bar3/hh⁸ has ommatidium phenotype, enhanceable by snama^PX1/mnm[+]

(Jones et al., 2006)

hh^bar3/hh⁸ has eye phenotype, enhanceable by l(2)rQ313[+]/snama^rQ313

(Jones et al., 2006)

hh^bar3/hh⁸ has ommatidium phenotype, enhanceable by l(2)rQ313[+]/snama^rQ313

(Jones et al., 2006)

hh^bar3/hh⁸ has eye phenotype, enhanceable by snama¹/mnm[+]

(Jones et al., 2006)

hh^bar3/hh⁸ has ommatidium phenotype, enhanceable by snama¹/mnm[+]

(Jones et al., 2006)

hh^bar3 has eye | anterior phenotype, enhanceable by foi[+]/foi^B1-089

(Pielage et al., 2004)

hh^bar3 has eye phenotype, enhanceable by oro¹/oro[+]

(Epps et al., 1997)

hh^bar3 has ommatidium phenotype, enhanceable by oro¹/oro[+]

(Epps et al., 1997)

hh^bar3 has eye phenotype, enhanceable by oro²/oro[+]

(Epps et al., 1997)

hh^bar3 has ommatidium phenotype, enhanceable by oro²/oro[+]

(Epps et al., 1997)

hh^bar3 has ommatidium phenotype, enhanceable by Ten-m[+]/Ten-m⁰⁵³⁰⁹

(Levine et al., 1997)

hh^bar3 has ommatidium phenotype, enhanceable by Df(3L)Ten-m-AL3/+

(Levine et al., 1997)

Suppressed by

Statement

Reference

hh^bar3/hh⁸ has ommatidium phenotype, suppressible by BRWD3^ram1

(D'Costa et al., 2006)

hh^bar3/hh⁸ has eye phenotype, suppressible by BRWD3^ram1

(D'Costa et al., 2006)

hh^bar3 has eye phenotype, suppressible by trr^+t12

(Sedkov et al., 2003)

hh^bar3 has eye phenotype, suppressible by ptc¹⁴/ptc^tuf-1

(Thomas and Ingham, 2003)

hh^bar3 has eye phenotype, suppressible by ptc¹⁶/ptc^tuf-1

(Thomas and Ingham, 2003)

hh^bar3 has eye phenotype, suppressible by ptc³⁴/ptc^tuf-1

(Thomas and Ingham, 2003)

hh^bar3 has eye phenotype, suppressible by ptc³⁷/ptc^tuf-1

(Thomas and Ingham, 2003)

hh^bar3 has eye phenotype, suppressible by ptc⁴⁷/ptc^tuf-1

(Thomas and Ingham, 2003)

hh^bar3 has eye phenotype, suppressible by ptc¹³/ptc^tuf-1

(Thomas and Ingham, 2003)

hh^bar3 has macrochaeta | ectopic phenotype, suppressible by sc^sisB-1

(Wrischnik et al., 2003)

hh^bar3 has macrochaeta | ectopic phenotype, suppressible by sc^M6

(Wrischnik et al., 2003)

hh^bar3 has ommatidium phenotype, suppressible by sca^UAS.cEa/Scer\GAL4^hs.2sev

(Chou and Chien, 2002)

hh^bar3 has phenotype, suppressible by amos^Roi-1

(Heberlein et al., 1993)

NOT suppressed by

Statement

Reference

hh^bar3 has macrochaeta | ectopic phenotype, non-suppressible by sc^sisB-2

(Wrischnik et al., 2003)

hh^bar3 has macrochaeta | ectopic phenotype, non-suppressible by sc^sisB-3

(Wrischnik et al., 2003)

Enhancer of

Statement

Reference

hh^bar3/hh^bar3 is an enhancer of eye phenotype of L²

(Singh et al., 2005)

hh[+]/hh^bar3 is an enhancer of ommatidium phenotype of sca^BP1/sca^BP2

(Chou and Chien, 2002)

Suppressor of

Statement

Reference

hh^bar3 is a suppressor of eye | ectopic | somatic clone phenotype of Scer\GAL4^Act5C.PI, ey^UAS.cHa

(Kango-Singh et al., 2003)

NOT Suppressor of

Statement

Reference

hh[+]/hh^bar3 is a non-suppressor of eye phenotype of L^fee

(Wang and Huang, 2009)

hh^bar3/hh^bar3 is a non-suppressor of ommatidium phenotype of nmo^j147-1/nmo^P1

(Chou and Chien, 2002)

Other

Statement

Reference

CycE², hh^bar3 has wing phenotype

(Wang et al., 2017)

BRWD3^s5349/BRWD3[+], hh^bar3/hh⁸ has rhabdomere phenotype

(D'Costa et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

The presence of the apt¹⁶⁷/+ mutation enhances the severity of the wing defects seen in hh^bar3/hh^bar3 mutant adults, showing further reduced wing area between L3 and L4, small wings and blistering.

A subset of CycE²/+, hh^bar3/hh^bar3 double mutant flies show smaller, blistered wings, as compared to controls.

(Wang et al., 2017)

The reduced eye size of L^fee flies is not restored by hh^bar3/+.

(Wang and Huang, 2009)

BRWD3^ram1 suppresses the small, rough eye phenotype of hh⁸/hh^bar3 flies.

The presense of BRWD3^s5349/+ does not significantly affect the size or roughness of eye in hh⁸/hh^bar3 flies. However, retinal sections reveal the presence of small rhabdomeres in these eyes, which are not present in the eyes of hh⁸/hh^bar3 animals in the absence of BRWD3^s5349/+.

(D'Costa et al., 2006)

L²/+ ; hh^bar3/hh^bar3 flies show complete elimination of the eye.

(Singh et al., 2005)

When ey^Scer\UAS.cHa clones (driven by Scer\GAL4^Act5C.PI) are made in the wing disc in a hh^bar3 background no ectopic eyes are seen.

(Kango-Singh et al., 2003)

The hh^bar3 eye phenotype is suppressed by ptc¹³/ptc^tuf-1 and partially suppressed by ptc^tuf-1 in trans to ptc¹⁶, ptc¹⁴, ptc³⁴, ptc³⁷ or ptc⁴⁷.

(Thomas and Ingham, 2003)

Ectopic macrochaetae on the scutellum of hh^bar3 homozygotes is suppressed by 75% in sc^M6 hemizygotes, but is only very weakly suppressed by hemizygous sc^sisB-1. This phenotype is not suppressed by hemizygous sc^sisB-2 or sc^sisB-3.

(Wrischnik et al., 2003)

The over-rotation of ommatidia seen in hh^bar3 mutant eye discs and adult eyes, is suppressed by sca^Scer\UAS.cEa; Scer\GAL4^hs.2sev.

(Chou and Chien, 2002)

The reduced eye phenotype is dominantly enhanced by oro¹ and oro². The eyes of oro¹/+ ; hh^bar3/hh^bar3 flies contain approximately 9 rows vertical rows of ommatidia from the posterior end. The eyes of ptc⁹/+ ; hh^bar3/hh^bar3 and ptc⁹/oro¹ ; hh^bar3/hh^bar3 flies are similar in size, suggesting that ptc is epistatic to oro with respect to effects on hh^bar3.

(Epps et al., 1997)

Df(3L)Ten-m-AL3/+ and Ten-m⁰⁵³⁰⁹/+ each enhance the loss of ommatidia phenotype which is seen in hh^bar3 homozygotes.

(Levine et al., 1997)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

hh^bar3 is rescued by hh^UAS.Tag:HA/Scer\GAL4^GMR.PF

(Tokhunts et al., 2010)

hh^bar3 is rescued by hh^N.UAS.Tag:HA/Scer\GAL4^GMR.PF

(Tokhunts et al., 2010)

hh^bar3 is rescued by hh^{U.NHA.UAS.Tag:HA}/Scer\GAL4^GMR.PF

(Tokhunts et al., 2010)

hh^bar3 is rescued by hh^UAS.cAa/Scer\GAL4^GMR.PF

(Chou and Chien, 2002)

Comments

Images (0)

Mutant

Wild-type

Stocks (5)

Bloomington

1376

hh^bar3 tx¹

1450

ru¹ hry¹ red¹ cv-c¹ Sb^sbd-2 sr¹ e^s hh^bar3 tx¹ ca¹/TM6, h^*

Kyoto

105915

hh^bar3

106293

hh^bar3 tx¹

106318

ru¹ hry¹ red¹ cv-c¹ Sb^sbd-2 sr¹ e^s hh^bar3 tx¹ ca¹/TM6, h^*

Notes on Origin

Discoverer

Comments

Eye specific allele.

(Chiang and Beachy, 1994)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

bar-3

(Ma et al., 1996, Lee et al., 1992)

hh1

(Hoyle et al., 2000)

hh^bar-3

(Held, 1993)

hh^bar3

(Ehlers et al., 2024, Gude et al., 2023, Manikowski et al., 2023, Kastl et al., 2018, Wang et al., 2017, Jones et al., 2006, Rogers et al., 2005)

Name Synonyms

Secondary FlyBase IDs

FBal0005462

References (60)

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