FlyBase Allele Report: Dmel\snama[PX1]

General Information

Symbol

Dmel\snama^PX1

Species

D. melanogaster

Name

FlyBase ID

FBal0193193

Feature type

allele

Associated gene

Dmel\snama

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

loss of function allele

(Jones et al., 2006)

Mutagen

Delta2-3 transposase

(Jones et al., 2006)

Progenitor genotype

snama^rQ313

(Jones et al., 2006)

Cytology

Description

Imprecise excision of the insertion in mnm^rQ313, resulting in a deletion of 1488bp extending distally from the site of the insertion. The deletion removes the start of the open reading frame and three of the most conserved protein domains.

(Jones et al., 2006)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

2R:24,087,324..24,088,811 [+]

Comment:

1488bp deletion resulting from the imprecise excision of P{PZ}snamar^Q313 The deletion extends distally from the site of the insertion.

(Jones et al., 2006)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

FlyBase_internaltransgene_features:cell lethal:cell lethal | somatic clone

(Jones et al., 2006)

lethal - all die during embryonic stage | recessive

(Jones et al., 2006)

some die during embryonic stage | recessive

(Jones et al., 2006)

visible | somatic clone

(Jones et al., 2006)

Phenotype Manifest In

eye | somatic clone

(Jones et al., 2006)

Detailed Description

Statement

Reference

Homozygous clones in the eye (induced using the "eyFLP" method) are largely absent from the adult retina, resulting in scars in the adult eye. In late larval discs, small homozygous clones are seen, which express the differentiation marker elav.

Homozygous clones examined in wandering third instar larvae 24 hours after being induced are frequent, small and similar in size (about 2 to 4 cells) to their adjacent wild-type twin spots. By 48 hours after induction, homozygous clones and wild-type twin spots are seen posterior to the furrow, but anterior to the furrow the homozygous clones are lost, while the wild-type twin spots grow larger. By 72 hours after induction, homozygous clones are rare, small and found only posterior to the furrow, while the wild-type twin spots are large and often not associated with a homozygous clone. By 96 hours after induction, only the wild-type twin spots are seen. Mutant cells that do persist to the pupal stage do not appear any smaller than their wild-type neighbours and can differentiate into morphologically normal photoreceptors and accessory cells.

Homozygous cells induced in the wing disc and examined 24 hours later (at the third larval instar stage) show a shift towards higher nucleic acid content compared to wild-type cells when analysed using FACS analysis and appear to accumulate DNA beyond 4N. The forward scatter profiles of the mutant and wild-type cells are similar.

(Jones et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

snama^PX1/mnm[+] is an enhancer of visible phenotype of hh^bar3/hh⁸

(Jones et al., 2006)

Suppressor of

Statement

Reference

snama^PX1/mnm[+] is a suppressor of visible phenotype of N^spl-1

(Jones et al., 2006)

snama^PX1/mnm[+] is a suppressor of visible phenotype of dpp^d-blk

(Jones et al., 2006)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

snama^PX1 has eye disc | somatic clone phenotype, non-enhanceable by BacA\p35^GMR.PH

(Jones et al., 2006)

snama^PX1 has eye disc | somatic clone phenotype, non-enhanceable by Scer\GAL4^hh-Gal4/BacA\p35^UAS.cHa

(Jones et al., 2006)

NOT suppressed by

Statement

Reference

snama^PX1 has eye disc | somatic clone phenotype, non-suppressible by BacA\p35^GMR.PH

(Jones et al., 2006)

snama^PX1 has eye disc | somatic clone phenotype, non-suppressible by Scer\GAL4^hh-Gal4/BacA\p35^UAS.cHa

(Jones et al., 2006)

Enhancer of

Statement

Reference

snama^PX1/mnm[+] is an enhancer of eye phenotype of hh^bar3/hh⁸

(Jones et al., 2006)

snama^PX1/mnm[+] is an enhancer of ommatidium phenotype of hh^bar3/hh⁸

(Jones et al., 2006)

Suppressor of

Statement

Reference

snama^PX1/mnm[+] is a suppressor of eye phenotype of N^spl-1

(Jones et al., 2006)

snama^PX1/mnm[+] is a suppressor of ommatidium phenotype of N^spl-1

(Jones et al., 2006)

snama^PX1/mnm[+] is a suppressor of eye phenotype of dpp^d-blk

(Jones et al., 2006)

snama^PX1/mnm[+] is a suppressor of ommatidium phenotype of dpp^d-blk

(Jones et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

The reduced size of mnm^PX1 mutant clones in the eye disc is unaffected by BacA\p35^GMR.PH.

The small size of mnm^PX1 clones in the eye disc 48 hours after induction is not rescued by expression of BacA\p35^Scer\UAS.cHa under the control of Scer\GAL4^hh-Gal4.

(Jones et al., 2006)

Complementation and Rescue Data

Rescued by

snama^PX1 is rescued by snama^+t14.7

(Jones et al., 2006)

Not rescued by

snama^PX1 is not rescued by snama^tmut4

(Jones et al., 2006)

snama¹/snama^PX1 is not rescued by snama^tmut4

(Jones et al., 2006)

snama^rQ313/snama^PX1 is not rescued by snama^tmut4

(Jones et al., 2006)