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General Information
Symbol
Dmel\scM6
Species
D. melanogaster
Name
FlyBase ID
FBal0046403
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Nucleotide change:

C396516T

Reported nucleotide change:

C1000T

Amino acid change:

Q114term | sc-PA

Reported amino acid change:

Q114term

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Amino acid replacement: Q114term.

Nonsense mutation at position 114.

Nonsense mutation.

Nucleotide substitution: C1000T.

Amino acid replacement: ??term.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Females homozygous for scM6 fail to activate the SxlPe (establishment) promoter and subsequently die from dosage compensation upsets.

Hemizygous scM6 flies are sterile and lack some sensory bristles. scM6/+ female flies have a very low frequency of missing anterior postalar and posterior supra-alar bristles.

At 18oC 12% of expected scsisB-2 homozygotes survive to adulthood, but this drops to less than 0.2% when these animals are raised at 25oC. In contrast, hemizygous males are fully viable. With variable penetrance scM6 homozygous adults lack all dorsal head bristles apart from the posterior vertical bristles, and all bristles of the dorsal thorax apart from the posterior notoplural, posterior dorsocentral and scutellar bristles. With variable penetrance scM6/In(1)sc8Lsc4R adults lack all dorsal head bristles apart from the anterior vertical bristles, and all bristles of the dorsal thorax apart from the dorsocentral and bristles. With variable penetrance scM6/Df(1)sc19 adults lack all dorsal head bristles and all dorsal thoracic bristles.

The bristle phenotype seen female D.simulans/D.melanogaster hybrids is unaffected by the addition of scM6 at 18oC. At 25oC there is a small general low level increase in bristle loss.

Homozygous embryos develop MP2 neuroblast normally.

Females are lethal, most likely because of the absence of sc+ function in the blastoderm. Homozygotes display a reduction in the presence of certain notum bristles. In an ac- background (In(1)y3PLsc8R) the phenotype was improved, only the anterior dorsocentral bristle, posterior supraalar bristle and microchaetae are reduced in numbers.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement
Reference

scM6 has lethal | recessive | female | heat sensitive phenotype, suppressible | partially by acXSE

scM6 has lethal | recessive | female | heat sensitive phenotype, suppressible by Mdoa\scXSE

Other
Phenotype Manifest In
Enhanced by
Statement
Reference

scM6 has anterior postalar bristle phenotype, enhanceable by accami/ac[+]

scM6 has posterior supraalar bristle phenotype, enhanceable by accami/ac[+]

Suppressor of
Statement
Reference

scM6/sc[+] is a suppressor | partially of macrochaeta | ectopic phenotype of emc2

scM6 is a suppressor | partially of macrochaeta | ectopic phenotype of emc2

scM6 is a suppressor | partially of macrochaeta | ectopic phenotype of hhbar3

Other
Additional Comments
Genetic Interactions
Statement
Reference

scM6/+, accami/+ double mutants have a significantly increased loss of the anterior postalar, posterior supra-alar and posterior notopleural bristles compared to scM6/+ single mutants.

The ectopic macrochaetae phenotype in emc2/Df(3L)Ar11 animals is almost completely suppressed by scM6/scM6, but is only weakly suppressed by scM6/+. Ectopic macrochaetae on the scutellum of hhbar3 homozygotes is suppressed by 75% in scM6 hemizygotes.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Partially rescued by

scM6 is partially rescued by sct.3XKO.XSE

scM6 is partially rescued by sct.sisB2.XSE

Comments

Expression of sc+t.XSE fully rescues scM6 with respect to female-specific lethality.

Expression of sc1xXSE.CAGGcAG fully rescues scM6 with respect to female-specific lethality.

Expression of sc1xXSE.CAGGTAG fails to rescue scM6 with respect to female-specific lethality, with only 35% viability.

Expression of sc3xXSE.CAGGTAG fails to rescue scM6 with respect to female-specific lethality, with only 3% viability.

Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (10)