Construct: sc+t.XSE in which the following sequences immediately upstream of the sc ORF have been mutated as indicated: CTACCTG -> GTAGGTG ; CTACCTG -> AATAAAC ; CAGGTAG -> CATTTAT (order of these sites is 5' -> 3' with respect to the sc ORF.)
sct.3XKO.XSE partially rescues scM6
The female lethality of scM6 homozygotes is rescued by sct.3XKO.XSE.