Open Close
General Information
Symbol
Dmel\hthUAS.cPa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Pai
FlyBase ID
FBal0089223
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-hth, UAS-hth12
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

A hth cDNA fragment containing the entire open reading frame is expressed under the control of UAS regulatory sequences.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In

alula & wing sensillum, with Scer\GAL4Bx-MS1096

leg & trichome | somatic clone, with Scer\GAL4Act5C.PI

microchaeta & scutum, with Scer\GAL4MS248

Detailed Description
Statement
Reference

Larvae expressing hthUAS.cPa under the control of Scer\GAL4Hml.Δ do not show changes in lymph gland size or morphology, or in the number of lymph gland hemocytes, as compared to controls.

Expression of hthScer\UAS.cPa under the control of Scer\GAL4Act.PU in somatic clones in the brain optic lobes triggers conversion of p-IPC (proximal inner proliferation centre - gives rise to migratory progenitors) to neuroblasts.

Expression of hthScer\UAS.cPa under the control of Scer\GAL4ct.CtB in embryos results in Malpighian tubule defects, with a variable pattern of loop formation, as compared to wild type. Expression of hthScer\UAS.cPa under the control of Scer\GAL4ct.CtB in third instar larvae results morphological changes in the Malpighian tubules, resulting in difficulty distinguishing between cells of the three segments based on morphology as all cells appear similar, in contrast to wild type tubules.

Misexpression of hthScer\UAS.cPa in the eye under the control of Scer\GAL4ey.PH suppresses eye fate in the eye imaginal disc and adult eye.

Clones in the antennal disc expressing hthScer\UAS.cPa under the control of Scer\GAL4Act5C.PU tend to be excluded from A3, with their borders often running along the A2-A3 boundary.

Expression of hthScer\UAS.cPa under the control of Scer\GAL4twi.PG in embryos results in a reduction in the number of cells in the posterior signaling centre (PSC) of the lymph gland, while the size of the lymph gland remains relatively normal.

Expression of hthScer\UAS.cPa under the control of Scer\GAL4ap-md544 results in a reduction in size of the scutum and most the pattern elements of the scutum, such as bristles, are missing. Expression of hthScer\UAS.cPa under the control of Scer\GAL4MS248 results in a reduction of the notum, which affects mostly the scutum territory (the scutum develops very few microchaetae and the dorsocentral macrochaetae are missing).

Scer\GAL4Dll-md23/hthScer\UAS.cPa flies have truncated legs in which all distal segments are missing. In Scer\GAL4bab1.A/hthScer\UAS.cPa flies, the tarsal segments 2-4 are missing or reduced. In Scer\GAL4ap-md544/hthScer\UAS.cPa flies the fourth tarsal segment is missing and in males an ectopic sex comb tooth is present in a distal position. Clones of hth-expressing cells (hthScer\UAS.cPa, Scer\GAL4Ubx.PdC) exhibit poor growth and the majority are eliminated. The effects on growth and survival are more severe in clones in the distal leg than those located proximal to the trochanter. In all the distal leg segments (femur, tibia, metatarsus and tarsi), hthScer\UAS.cPa, Scer\GAL4Ubx.PdC clones differentiate abnormally, often forming vesicles that sort out from the surrounding territory. They are recovered with low frequency and are associated with pattern abnormalities in the wild-type cells close to the clones. The type of bristles that the surviving hth expressing clones differentiate is unlike those of the region in which they are found, suggesting a change in fate. A general feature of these clones is that they appear to induce the formation of proximal structures in a distal position. In many instances, alterations in the distal leg of flies in which clones have been induced are not associated with visible hth-expressing clones, probably reflecting a non-autonomous effect of a clone that has been eliminated. Legs of Scer\GAL4C-765, hthScer\UAS.cPa flies show different degrees of proximalisation which correlate with the temperature; at 17C, there is a slight effect, whereas at 28C, there is extreme proximalisation and often the leg is reduced to a single segment.

Expression of hthScer\UAS.cPa under the control of Scer\GAL4bi-omb-Gal4 results in suppression of eye development. Expression of hthScer\UAS.cPa under the control of Scer\GAL4bi-omb-Gal4 does not result in splitting of the wing pouch.

Expression of hthScer\UAS.cPa under the control of Scer\GAL4dpp.blk1 results in a reduction in antennal segment 3 and a loss of the arista. Proximal leg structures are intact, but distal structures are malformed and the claws are not seen.

Expression of hthScer\UAS.cPa under the control of Scer\GAL4ptc-559.1 results in bifurcated adult legs.

The wing pouch does not develop in flies expressing hthScer\UAS.cPa under the control of Scer\GAL4nub-AC-62, Scer\GAL4Bx-MS1096 or Scer\GAL4C-765 but proximal structures like the sclerites or costa are normal. The number of alula bristles in flies expressing hthScer\UAS.cPa under the control of Scer\GAL4Bx-MS1096 is higher than in wild-type flies. Expression of hthScer\UAS.cPa under the control of Scer\GAL4ap-md544 prevents growth of the wing. Expression of hthScer\UAS.cPa under the control of Scer\GAL4bi-omb-Gal4 results in a wing which lacks the distal medial region. Wing discs are reduced in size in larvae expressing hthScer\UAS.cPa under the control of Scer\GAL4bi-omb-Gal4. Expression of hthScer\UAS.cPa under the control of Scer\GAL4C-765 at low temperatures only affects the distal wing, while and higher temperatures both the distal and medial regions are affected, so that in the strongest phenotypes only proximal wing elements remain. Few clones expressing hthScer\UAS.cPa under the control of Scer\GAL4Ubx.PdC can be recovered in the differentiated cuticle. Many of those that can be seen are in the proximal wing. In the margin, they are associated with the loss of marginal elements, while those located closer to the A/P border are associated with the loss of large portions of the middle of the wing blade. A few clones are found near the medial DV wing margin; they only differentiate wing trichomes, but appear to induce local duplications of wing margin elements.

Clones in the leg expressing hthScer\UAS.cPa under the control of Scer\GAL4Act5C.PI invaginate to form a vesicle in a cell-autonomous manner. The orientation of bristles and hairs is reversed near the clone. Clones in the distal part of the leg sometimes form thick socketted bristles without bracts, which are characteristics of the bristles in the proximal part of the leg. Clones expressing hthScer\UAS.cPa under the control of Scer\GAL4Act5C.PI in the distal region of the leg disc are round in shape and have smooth borders.

Clones expressing hthScer\UAS.cPa under the control of Scer\GAL4Act5C.PP in the distal region of the leg disc segregate out of the disc epithelium. Vesicles of invaginated tissue are seen in the tarsus and tibia in a high proportion of the legs of adults in which clones have been induced during the larval stages.

When Scer\GAL4dpp.blk1 drives the expression of hthScer\UAS.cPa, the eye disc and adult eye are reduced in size, the arista are always missing, and the third antennal segment is enlarged and often duplicated. The duplicated segment is mirror symmetric as judged from the scar left by the missing arista. The proximal tarsal segment is also often duplicated.

Expression of hthScer\UAS.cPa under the control of Scer\GAL4dpp.blk1 completely suppresses eye development. Clones in the eye expressing hthScer\UAS.cPa under the control of Scer\GAL4Act5C.PP disrupt the propogation of the morphogenetic furrow (MF) when they are located anterior to the MF. This disruption is detected in adult flies as a scar running in the anterior-posterior direction. In some cases the eye is split into a dorsal part and a ventral part.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhancer of
Suppressor of
NOT Suppressor of
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

The ectopic Mi1-like neurons induced by expression of bshScer\UAS.PB under the control of Scer\GAL4vvl-GAL4 are morphologically more similar to endogenous Mi1 neurons if the animals are also co-expressing hthScer\UAS.cPa.

Expression of hthScer\UAS.cPa almost completely suppresses the ectopic eye phenotype seen in adult flies expressing eyScer\UAS.cHa under the control of Scer\GAL4dpp.blk1. The photoreceptor induction seen in the wing discs is also suppressed.

Misexpression of hthScer\UAS.cPa in a L2 heterozygous eye under the control of Scer\GAL4ey.PH results in strong enhancement of the loss-of-ventral-eye phenotype to a 'no-eye' phenotype.

Flies expressing both hthScer\UAS.cPa and eygScer\UAS.cAa under the control of Scer\GAL4MS248 have duplicated notum structures, but the duplicated structures do not contain macrochaetae.

The defect in the embryonic tritocerebrum caused by expression of UbxScer\UAS.cCa under the control of Scer\GAL4sca-537.4 is completely suppressed if the embryos are also coexpressing both exdScer\UAS.NLS.T:Zzzz\FLAG and hthScer\UAS.cPa. The defect in the embryonic brain caused by expression of UbxYAAA.Scer\UAS under the control of Scer\GAL4sca-537.4 is not suppressed if the embryos are also coexpressing both exdScer\UAS.NLS.T:Zzzz\FLAG and hthScer\UAS.cPa.

Coexpression of both hthScer\UAS.cPa and tshScer\UAS.cGa under the control of Scer\GAL4dpp.blk1 results in complete eye loss and no antennal eye is produced.

When cncB.hs and hthScer\UAS.cPa (driven by Scer\GAL4hs.PB) are both ectopically expressed throughout the embryo, a reduction in severity of all aspects of the cncB.hs phenotype is observed. Although still resulting in embryonic lethality, most head skeletal elements are close to wild-type and mouth hooks are seen in all embryos.

Expression of hthScer\UAS.cPa partially suppresses the antenna to leg transformation phenotypes produced by expression of ScrScer\UAS.cAa, AntpScer\UAS.cMb, UbxScer\UAS.cCa, and abd-A1dB5.Scer\UAS when driven by Scer\GAL4dpp.blk1. The antennae produced are aristaless and occasionally duplicated, similar to the hthScer\UAS.cPa phenotype.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

Expression of hthScer\UAS.cPa under the control of Scer\GAL4hth.MpT partially rescues the Malpighian tubule morphology and organization of hth64-1/hth64-1 embryos, but does not fully rescue the Malpighian tubule anterior migration defect in these embryos.

Expression of hthScer\UAS.cPa under the control of Scer\GAL4how-24B, but not Scer\GAL448Y, Scer\GAL469B, Scer\GAL4ct.CtB or Scer\GAL4bap.PU, partially rescues the Malpighian tubule anterior migration defect of hth64-1/hth64-1 embryos. Expression of hthScer\UAS.cPa under the control of Scer\GAL4how-24B also partially rescues the stellate cell number decrease seen in hth64-1/hth64-1 embryos.

Addition of hthScer\UAS.ΔHD to hthScer\UAS.cPa, Scer\GAL4dpp.blk1 flies partially suppresses the loss of eye phenotype normally seen in these flies. hthScer\UAS.ΔMH, hthScer\UAS.KI or hthScer\UAS.KΔHD do not have this effect.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
hthScer\UAS.cPa
hthUAS.cPa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Pai
Secondary FlyBase IDs
    References (37)