FlyBase Allele Report: Hsap\CDKN1A[UAS.cHa]

Ectopic expression of Hsap\CDKN1A^Scer\UAS.cHa under the regulation of Scer\GAL4^sca-537.4 leads to a reduction (from 5-6 to 2-4), but not the complete loss of neuronal cells in NB6-4t.

(Berger et al., 2005)

The salivary glands of Hsap\CDKN1A^Scer\UAS.cHa; Scer\GAL4^ptc-559.1 larvae are small and their cells do not have endoreplicated DNA.

(Pierce et al., 2004)

Expression of Hsap\CDKN1A^Scer\UAS.cHa under the control of Scer\GAL4^ey.PH results in smaller third larval instar eye discs than normal and eyes which are reduced in size. Flies expressing Hsap\CDKN1A^Scer\UAS.cHa under the control of Scer\GAL4^hs.2sev have wild-type eyes.

(Tseng and Hariharan, 2002)

When expression is driven in the wing with Scer\GAL4^en-e16E the resulting phenotype is similar to that caused by RacGAP50C^{ΔE1E.Scer\UAS} in that cells are enlarged, hair polarity is altered and apotosis abnormally elevated.

(Sotillos and Campuzano, 2000)

When expression is driven by Scer\GAL4^GMR.PF BrdU staining is abolished posterior to the morphogenetic furrow.

(Huang et al., 1999)

Flies carrying two copies of Hsap\CDKN1A^Scer\UAS.cHa and Scer\GAL4^dpp.blk1, raised at 18^oC, are morphologically normal, except for the loss of the anterior cross vein and part of the L3 wing vein. Cells in the L3-L4 intervein region are larger than normal.

(Karim and Rubin, 1998)

Expression of Hsap\CDKN1A^Scer\UAS.cHa under the control of Scer\GAL4¹¹⁵¹ strongly inhibits proliferation of the imaginal myoblasts in the wing imaginal disc. The persistent thoracic larval muscles escape histolysis in these animals, as in wild-type development, but the larval muscles do not split during pupation, in contrast to wild-type. Few, if any, myoblasts are associated with the persistent larval muscles. Early metamorphic events in the larval motor nerves occur normally.

(Roy and VijayRaghavan, 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Hsap\CDKN1A^UAS.cHa, Scer\GAL4^en-e16E has increased cell death phenotype, suppressible | partially by CycE^UAS.cLa, Scer\GAL4^en-e16E

(Sotillos and Campuzano, 2000)

Hsap\CDKN1A^UAS.cHa, Scer\GAL4^en-e16E has increased cell death phenotype, suppressible | partially by tum^UAS.cSa, Scer\GAL4^en-e16E

(Sotillos and Campuzano, 2000)

Hsap\CDKN1A^UAS.cHa, Scer\GAL4^en-e16E has abnormal planar polarity phenotype, suppressible | partially by CycE^UAS.cLa, Scer\GAL4^en-e16E

(Sotillos and Campuzano, 2000)

Hsap\CDKN1A^UAS.cHa, Scer\GAL4^en-e16E has abnormal planar polarity phenotype, suppressible | partially by tum^UAS.cSa, Scer\GAL4^en-e16E

(Sotillos and Campuzano, 2000)

Hsap\CDKN1A^UAS.cHa, Scer\GAL4^en-e16E has visible phenotype, suppressible | partially by tum^UAS.cSa, Scer\GAL4^en-e16E

(Sotillos and Campuzano, 2000)

Hsap\CDKN1A^UAS.cHa, Scer\GAL4^en-e16E has visible phenotype, suppressible | partially by CycE^UAS.cLa, Scer\GAL4^en-e16E

(Sotillos and Campuzano, 2000)

Enhancer of

Statement

Reference

Hsap\CDKN1A^UAS.cHa/Scer\GAL4^ey.PH is an enhancer of abnormal size phenotype of L^fee

(Wang and Huang, 2009)

Hsap\CDKN1A^UAS.cHa, Scer\GAL4^GMR.PF is an enhancer of increased occurrence of cell division | larval stage phenotype of Scer\GAL4^GMR.PF, Src42A^UAS.cPa

(Pedraza et al., 2004)

Hsap\CDKN1A^UAS.cHa, Scer\GAL4^GMR.PF is an enhancer of increased occurrence of cell division | larval stage phenotype of Scer\GAL4^GMR.PF, Src64B^UAS.cPa

(Pedraza et al., 2004)

NOT Enhancer of

Statement

Reference

Hsap\CDKN1A^UAS.cHa, Scer\GAL4^GMR.PF is a non-enhancer of increased cell death phenotype of Scer\GAL4^GMR.PF, Src42A^UAS.cPa

(Pedraza et al., 2004)

Hsap\CDKN1A^UAS.cHa, Scer\GAL4^GMR.PF is a non-enhancer of increased cell death phenotype of Scer\GAL4^GMR.PF, Src64B^UAS.cPa

(Pedraza et al., 2004)

Suppressor of

Statement

Reference

Hsap\CDKN1A^UAS.cHa is a suppressor of increased occurrence of cell division phenotype of Ras85D^V12.UAS, Scer\GAL4^dpp.blk1

(Karim and Rubin, 1998)

Hsap\CDKN1A^UAS.cHa is a suppressor of hyperplasia | larval stage phenotype of Ras85D^V12.UAS, Scer\GAL4^dpp.blk1

(Karim and Rubin, 1998)

NOT Suppressor of

Statement

Reference

Hsap\CDKN1A^UAS.cHa, Scer\GAL4^Act88F.PD is a non-suppressor of visible phenotype of E2f1^UAS.cNa, Scer\GAL4^Act88F.PD

(Morris et al., 2006)

Hsap\CDKN1A^UAS.cHa, Scer\GAL4^GMR.PF is a non-suppressor of increased cell death phenotype of Scer\GAL4^GMR.PF, Src64B^UAS.cPa

(Pedraza et al., 2004)

Hsap\CDKN1A^UAS.cHa, Scer\GAL4^GMR.PF is a non-suppressor of increased cell death phenotype of Scer\GAL4^GMR.PF, Src42A^UAS.cPa

(Pedraza et al., 2004)

Phenotype Manifest In

Suppressed by

Statement

Reference

Hsap\CDKN1A^UAS.cHa, Scer\GAL4^en-e16E has wing & cell phenotype, suppressible | partially by CycE^UAS.cLa, Scer\GAL4^en-e16E

(Sotillos and Campuzano, 2000)

Hsap\CDKN1A^UAS.cHa, Scer\GAL4^en-e16E has wing & cell phenotype, suppressible | partially by tum^UAS.cSa, Scer\GAL4^en-e16E

(Sotillos and Campuzano, 2000)

Hsap\CDKN1A^UAS.cHa, Scer\GAL4^en-e16E has wing & trichome phenotype, suppressible | partially by CycE^UAS.cLa, Scer\GAL4^en-e16E

(Sotillos and Campuzano, 2000)

Hsap\CDKN1A^UAS.cHa, Scer\GAL4^en-e16E has wing & trichome phenotype, suppressible | partially by tum^UAS.cSa, Scer\GAL4^en-e16E

(Sotillos and Campuzano, 2000)

Hsap\CDKN1A^UAS.cHa, Scer\GAL4^en-e16E has wing phenotype, suppressible | partially by CycE^UAS.cLa, Scer\GAL4^en-e16E

(Sotillos and Campuzano, 2000)

Hsap\CDKN1A^UAS.cHa, Scer\GAL4^en-e16E has wing phenotype, suppressible | partially by tum^UAS.cSa, Scer\GAL4^en-e16E

(Sotillos and Campuzano, 2000)

Enhancer of

Statement

Reference

Hsap\CDKN1A^UAS.cHa/Scer\GAL4^ey.PH is an enhancer of eye phenotype of L^fee

(Wang and Huang, 2009)

Hsap\CDKN1A^UAS.cHa, Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Scer\GAL4^GMR.PF, Src42A^UAS.cPa

(Pedraza et al., 2004)

Hsap\CDKN1A^UAS.cHa, Scer\GAL4^GMR.PF is an enhancer of eye disc | larval stage phenotype of Scer\GAL4^GMR.PF, Src42A^UAS.cPa

(Pedraza et al., 2004)

Hsap\CDKN1A^UAS.cHa, Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Scer\GAL4^GMR.PF, Src64B^UAS.cPa

(Pedraza et al., 2004)

Suppressor of

Statement

Reference

Hsap\CDKN1A^UAS.cHa, Scer\GAL4^Cg.PA is a suppressor of embryonic/larval hemocyte | increased number phenotype of Ras85D^V12.UAS, Scer\GAL4^Cg.PA

(Asha et al., 2003)

NOT Suppressor of

Statement

Reference

Hsap\CDKN1A^UAS.cHa, Scer\GAL4^Act88F.PD is a non-suppressor of wing blade phenotype of E2f1^UAS.cNa, Scer\GAL4^Act88F.PD

(Morris et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Expression of Hsap\CDKN1A^Scer\UAS.cHa under the control of Scer\GAL4^ey.PH in a L^fee mutant background does not restore eye size, and flies have small eyes.

(Wang and Huang, 2009)

dm^Scer\UAS.cZa does not rescue salivary gland size in Hsap\CDKN1A^Scer\UAS.cHa; Scer\GAL4^ptc-559.1 larvae, but does rescue endoreplication in this tissue to a limited degree.

(Pierce et al., 2004)

The metastatic behaviour seen in clones in the eye disc which are homozygous for scrib¹ and are also expressing Ras85D^V12.Scer\UAS under the control of Scer\GAL4^Act5C.PI is not suppressed if they are co-expressing Hsap\CDKN1A^Scer\UAS.cHa; although the tumour size is decreased, mutant cells can still spread into the ventral nerve cord.

(Pagliarini and Xu, 2003)

Scer\GAL4^dpp.blk1-mediated expression of one copy of Hsap\CDKN1A^Scer\UAS.cHa suppresses most, but not all, of Ras85D^V12.Scer\UAS-induced cell proliferation and hyperplastic growth seen in wing discs. The lethal phase is delayed but not suppressed. At 18^oC coexpression with Ras85D^V12.Scer\UAS results in the complete suppression of lethality associated with Ras85D expression. Morphologically, the wing is restored to its normal shape and size, although Hsap\CDKN1A^Scer\UAS.cHa only partially suppresses the extra wing vein material along L3. Larger wing cell size is completely suppressed.

(Karim and Rubin, 1998)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

J. de Nooij M.G. Wang I.K. Hariharan

(Tseng and Hariharan, 2002)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

Hsap\CDKN1A^Scer\UAS.cHa

Hsap\CDKN1A^UAS.cHa

P21

(Berger et al., 2005)

p21

(Pierce et al., 2004)

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Hariharan

(Karim and Rubin, 1998)

Secondary FlyBase IDs

References (15)

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