Simultaneous expression of E2f1Scer\UAS.cUa and DpScer\UAS.cDa under the control of Scer\GAL4ptc.PU in cells of the wing disc proper has no significant effect on the morphology or proliferation of the overlying peripodial membrane cells in third instar larvae.
Cells posterior to the second mitotic wave in the eye disc which are co-expressing E2fScer\UAS.cNa and DpScer\UAS.cDa under the control of Scer\GAL4GMR.PF undergo ectopic S phases. In addition, independently of the ectopic S phases, co-expression of E2fScer\UAS.cNa and DpScer\UAS.cDa under the control of Scer\GAL4GMR.PF results in the induction of apoptosis in the eye disc.
The addition of mir-11Scer\UAS.T:Disc\RFP-DsRed2 does not prevent the ectopic S phases which are induced posterior to the morphogenetic furrow by co-expression of E2fScer\UAS.cNa and DpScer\UAS.cDa under the control of Scer\GAL4GMR.PF. However, co-expression of mir-11Scer\UAS.T:Disc\RFP-DsRed2 does suppress the ability of co-expression of E2fScer\UAS.cNa and DpScer\UAS.cDa under the control of Scer\GAL4GMR.PF to induce apoptosis in the eye disc.
Scer\GAL4tub- and Scer\GAL4Act5C.PI-driven co-expression of E2fScer\UAS.cNa and DpScer\UAS.cDa in CycEAR95 follicle stem cell (FSC) clones also expressing CycEWX.Scer\UAS.cWa suppresses the CycEWX-associated defective FSC-maintenance phenotype.
10 day old brains from animals expressing DpScer\UAS.cDa and E2fScer\UAS.cNa under the control of the selective driver Scer\GAL4elav.Switch.PO display marked loss of cortical neurons and rarefaction of the underlying neuropil compared to controls.
Co-expression of DlScer\UAS.cJa results in a moderate but reproducible and statistically significant increase in the number of mitoses seen in eye discs expressing E2fScer\UAS.cNa and DpScer\UAS.cDa under the control of Scer\GAL4GMR.PF.
When E2fScer\UAS.cNa and DpScer\UAS.cDa are driven by Scer\GAL4ptc-559.1 cells in the wing disc overproliferate. Many of these cells drop out of the epithelial layer and have pycnotic nuclei. The addition of banEP3622 enhances the overproliferation phenotype seen in E2fScer\UAS.cNa, DpScer\UAS.cDa, Scer\GAL4ptc-559.1 animals. Fewer cells show pyknotic nuclei, though many cells still drop out of the epithelial layer.
Marked clones in the eye disc which are homozygous for scrib1 and are co-expressing DpScer\UAS.cDa, E2fScer\UAS.cNa and BacA\p35Scer\UAS.cHa under the control of Scer\GAL4Act5C.PI do not show metastatic behaviour.
Co-expression of both E2f1Scer\UAS.cNa and DpScer\UAS.cDa in starved larvae under the control of Scer\GAL4Act5C.PP is sufficient to drive most endoreplicating cells into S phase, as assayed by BrdU incorporation.
Additional expression of Hsap\YARS1E196K.UAS enhances the rough eye phenotype induced by the co-expression of E2f1UAS.cNa and DpUAS.cDa under the control of Scer\GAL4GMR.long, leading to a decrease in eye size.
Co-expression of E2fScer\UAS.cNa, DpScer\UAS.cDa and BacA\p35Scer\UAS.cHa under the control of Scer\GAL4vg.int2.1 rescues wing appendage growth in vg83b27 flies; wing blade features such as bristles, wing veins and margin are often seen in the rescued appendages.
Co-expression of E2fScer\UAS.cNa, DpScer\UAS.cDa and BacA\p35Scer\UAS.cHa under the control of Scer\GAL4vg.int2.1 does not wing blade growth in vgnull flies and only a slight increase in wing hinge growth is seen.