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General Information
Symbol
Dmel\pho1
Species
D. melanogaster
Name
FlyBase ID
FBal0011501
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
29
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

4.5kb Doc insertion into the coding region at codon 272, upstream of the zinc finger region of the protein.

Insertion components
Doc{}pho1
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference
Enhancer of
Statement
Reference

pho[+]/pho1 is an enhancer of visible phenotype of E2f1UAS.cNa, Scer\GAL4Act88F.PD

pho1 is an enhancer of visible phenotype of TrlR85, Ubx130

NOT Enhancer of
Statement
Reference

pho[+]/pho1 is a non-enhancer of visible | adult stage phenotype of Marcal1UAS.cBa, Scer\GAL4Tub.PU

pho[+]/pho1 is a non-enhancer of visible phenotype of DpUAS.cDa, E2f1UAS.cNa, Scer\GAL4GMR.PU

Suppressor of
Statement
Reference

pho[+]/pho1 is a suppressor of visible phenotype of E2f1dsRNA.UAS.cJa, Scer\GAL4GMR.PU

pho[+]/pho1 is a suppressor | partially of visible phenotype of E2f1dsRNA.UAS.cJa, Scer\GAL4ptc-559.1

NOT Suppressor of
Statement
Reference

pho[+]/pho1 is a non-suppressor of visible | adult stage phenotype of Marcal1UAS.cBa, Scer\GAL4Tub.PU

pho[+]/pho1 is a non-suppressor of visible phenotype of DpUAS.cDa, E2f1UAS.cNa, Scer\GAL4GMR.PU

Other
Phenotype Manifest In
Enhanced by
Statement
Reference

pho1 has phenotype, enhanceable by E(Pc)1

Suppressed by
Statement
Reference
NOT suppressed by
Statement
Reference

phocv/pho1 has antenna phenotype, non-suppressible by Hsap\YY1arm.PA

phocv/pho1 has sex comb phenotype, non-suppressible by Hsap\YY1arm.PA

phocv/pho1 has unguis phenotype, non-suppressible by Hsap\YY1arm.PA

Enhancer of
Statement
Reference

pho[+]/pho1 is an enhancer of wing phenotype of E2f1UAS.cNa, Scer\GAL4Act88F.PD

NOT Enhancer of
Statement
Reference

pho[+]/pho1 is a non-enhancer of wing vein | adult stage phenotype of Marcal1UAS.cBa, Scer\GAL4Tub.PU

pho[+]/pho1 is a non-enhancer of eye phenotype of DpUAS.cDa, E2f1UAS.cNa, Scer\GAL4GMR.PU

Suppressor of
Statement
Reference

pho[+]/pho1 is a suppressor of eye phenotype of E2f1dsRNA.UAS.cJa, Scer\GAL4GMR.PU

pho[+]/pho1 is a suppressor | partially of adult abdominal segment 4 | ectopic phenotype of E(z)Trm

pho[+]/pho1 is a suppressor | partially of adult abdominal segment 5 phenotype of E(z)Trm

pho[+]/pho1 is a suppressor | partially of adult mesothoracic segment | ectopic phenotype of E(z)Trm

pho[+]/pho1 is a suppressor | partially of adult metathoracic segment phenotype of E(z)Trm

pho[+]/pho1 is a suppressor | partially of adult prothoracic segment phenotype of E(z)Trm

pho1 is a suppressor of phenotype of wen.8

NOT Suppressor of
Statement
Reference

pho[+]/pho1 is a non-suppressor of wing vein | adult stage phenotype of Marcal1UAS.cBa, Scer\GAL4Tub.PU

pho[+]/pho1 is a non-suppressor of eye phenotype of DpUAS.cDa, E2f1UAS.cNa, Scer\GAL4GMR.PU

Other
Additional Comments
Genetic Interactions
Statement
Reference

The formation of ectopic wing veins observed in adult flies expressing Marcal1Scer\UAS.cBa under the control of Scer\GAL4tub.PU is not affected by combination with a single copy of pho1.

pho1, Spps1 double mutants die earlier in development than either single mutant: single mutants die as pharates, whereas double mutants die as third instar larvae or often at earlier stages. Double mutant larvae are often abnormally small and have misshapen imaginal discs.

The eye colour of flies homozygous for wGMR.eve.PRE300 is slightly darker in a pho1/+ background than in a wild-type background.

The eye colour of flies homozygous for weve.RR.PRE300 is considerably darker in a pho1/+ background than in a wild-type background.

The reduced wing phenotype of RYBPScer\UAS.cBa; Scer\GAL4sd-SG29.1 flies is enhanced by pho1/+.

TrlR85/Ubx130; pho1 flies show an enhancement of the Ubx130 phenotype in 18% of flies.

phol81A ; pho1 double mutants die as third instar larvae and fail to pupate. The brain is smaller than normal, the disks are misshapen and smaller than normal and the salivary gland polytene chromosomes are enlarged in double mutant third instar larvae.

11% of TrlR85/+ ; pho1/+ double heterozygotes have extra sex combs (on the 2nd or 3rd leg) but they do not show transformation of antenna to leg.

pho1/phocv suppresses the pairing-dependent silencing of wiab-7PRE.860 seen in flies homozygous for P{iab-7PRE-w.860}P24.

Mutant heterozygotes show a moderate suppression of the eye phenotype see in P{UAS-lacZ.Abd-B.5F24} leading to darker eyes.

The addition of pho1/phocv strongly suppresses the Pairing sensitive silencing effect seen in wMcp.10, wMcp.13,wMcp.scs'.12:15 and wMcp.scs'.11. The addition of pho1/phocv has no effect on the Pairing sensitive silencing effect seen in wMcp.11, and wMcp.scs'.14. The addition of pho1/phocv very strongly suppresses the Pairing sensitive silencing effect seen in wMcp.scs'.11 (P{Mcp-w#11}16/+, P{Mcp-w#11}102/+) double heterozygous insertions. The addition pho1/phocv very strongly suppresses the Pairing sensitive silencing effect seen in the wMcp.scs'.14/ wMcp.13 (P{Mcp-w#14}AP-2stummelbein/+, P{Mcp-w#13}116/+) double heterozygous insertion.

Has little or no effect on the phenotype of E2fGMR.PD DpGMR.PD BacA\p35GMR.PH flies.

Eye colour encoded by wen.8 is darker in pho1/pho1 or pho1/phocv background.

Xenogenetic Interactions
Statement
Reference

The formation of ectopic wing veins observed in adult flies expressing Hsap\SMARCAL1Scer\UAS.cBa under the control of Scer\GAL4Bx-MS1096 is not affected by combination with a single copy of pho1.

When heatshocked twice daily, Hsap\YY1Hsp70.T:Scer\GAL4 nearly completely corrects the antennal and sex comb defects in pho1/phocv mutant flies. Segmentation is largely normal, although pigmentation on tergite 5 is not always complete. Correction of claw structures is variable ranging from complete to partial correction.

Complementation and Rescue Data
Partially rescued by
Not rescued by

pho1 is not rescued by phoEGFP

pho1 is not rescued by phoEGFP

Comments

Expression of phoScer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4arm.PS partially rescues pho1 homozygotes: approximately 10% of rescued adult males show moderate A4-A5 transformations.

Two copies of phohs.T:Ecol\lexA produces healthy pho1/pho1 adults with one heat shock daily beginning 4 days after egg laying. The rescued pho1/pho1 males are fully fertile, but occasionally show sex comb bristles on the mesothoracic legs and excess pigmentation in the 4th abdominal tergite. The rescued pho1/pho1 females have severely impaired fertility that cannot be rescued with a paternal pho+ allele. Only 10% of embryos derived from pho1/pho1 females crossed to pho1/pho1 males hatch, even when heat shocked before blastoderm to express phohs.T:Ecol\lexA. These embryos show frequent and various segmental abnormalities but never show classical homeotic derepression phenotypes. The few larvae that hatch grow slowly and never reach the pupal stage, probably because evident head skeleton abnormalities preclude normal feeding.

Images (0)
Mutant
Wild-type
Stocks (3)
Notes on Origin
Discoverer

Isolated from: Bountiful, UT population.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (6)
References (33)