FlyBase Allele Report: Dmel\mir-998[UAS.cTa]

General Information

Symbol

Dmel\mir-998^UAS.cTa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Truscott

FlyBase ID

FBal0297897

Feature type

allele

Associated gene

Dmel\mir-998

Associated Insertion(s)

Carried in Construct

P{UAS-mir-998.T}

Also Known As

UAS-mir-998

Key Links

Transgenic product class

wild_type

(Truscott et al., 2014)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Truscott et al., 2014)

Mutagen

in vitro construct

(Truscott et al., 2014)

Progenitor genotype

Carried in construct

P{UAS-mir-998.T}

Cytology

Description

UASt regulatory sequences drive expression of mir-998. The construct consists of E2f1 intron 5 in which mir-11 has been replaced by a mir-1 pre mRNA scaffold containing a short hairpin miRNA targeting mCherry and mir-998 has been replaced by a mir-1 pre mRNA scaffold containing mir-998.

(Truscott et al., 2014)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

mir-998

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

mir-998^UAS.cTa, Scer\GAL4^{FRT.Rnor\Cd2.Act5C} is a suppressor | partially of increased cell death phenotype of Cbl^L.UAS, Rbf^120a, Scer\GAL4^{FRT.Rnor\Cd2.Act5C}

(Truscott et al., 2014)

mir-998^UAS.cTa, Scer\GAL4^{FRT.Rnor\Cd2.Act5C} is a suppressor | partially of increased cell death phenotype of Cbl^S.UAS, Rbf^120a, Scer\GAL4^{FRT.Rnor\Cd2.Act5C}

(Truscott et al., 2014)

mir-998^UAS.cTa/Scer\GAL4^{FRT.Rnor\Cd2.Act5C} is a suppressor of increased cell death phenotype of Rbf^120a

(Truscott et al., 2014)

NOT Suppressor of

Statement

Reference

mir-998^UAS.cTa/Scer\GAL4^{FRT.Rnor\Cd2.Act5C} is a non-suppressor of increased cell death phenotype of E2f1^UAS.cNa

(Truscott et al., 2014)

mir-998^UAS.cTa, Scer\GAL4^GMR.PU is a non-suppressor of increased cell death phenotype of Dp^UAS.cDa, E2f1^UAS.cNa, Scer\GAL4^GMR.PU

(Truscott et al., 2014)

Phenotype Manifest In

Suppressor of

Statement

Reference

mir-998^UAS.cTa, Scer\GAL4^{FRT.Rnor\Cd2.Act5C} is a suppressor | partially of eye disc phenotype of Cbl^L.UAS, Rbf^120a, Scer\GAL4^{FRT.Rnor\Cd2.Act5C}

(Truscott et al., 2014)

mir-998^UAS.cTa, Scer\GAL4^{FRT.Rnor\Cd2.Act5C} is a suppressor | partially of eye disc phenotype of Cbl^S.UAS, Rbf^120a, Scer\GAL4^{FRT.Rnor\Cd2.Act5C}

(Truscott et al., 2014)

mir-998^UAS.cTa/Scer\GAL4^{FRT.Rnor\Cd2.Act5C} is a suppressor of eye disc phenotype of Rbf^120a

(Truscott et al., 2014)

NOT Suppressor of

Statement

Reference

mir-998^UAS.cTa/Scer\GAL4^{FRT.Rnor\Cd2.Act5C} is a non-suppressor of wing phenotype of E2f1^UAS.cNa

(Truscott et al., 2014)

mir-998^UAS.cTa, Scer\GAL4^GMR.PU is a non-suppressor of eye disc phenotype of Dp^UAS.cDa, E2f1^UAS.cNa, Scer\GAL4^GMR.PU

(Truscott et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of mir-998^Scer\UAS.cTa does not suppress the apoptosis seen in the wings of newly eclosed flies expressing E2f1^Scer\UAS.cNa under the control of Scer\GAL4^Act88F.PD.

Generation of eye disc clones expressing mir-998^Scer\UAS.cTa suppresses the cell death seen in Rbf^120a mutant eye discs.

Expression of mir-998^Scer\UAS.cTa does not suppress the apoptosis seen in the eye disc in flies expressing expressing E2f1^Scer\UAS.cNa and Dp^Scer\UAS.cDa under the control of Scer\GAL4^GMR.PU.

The generation of Cbl^S.Scer\UAS mutant eye disc clones enhances the amount of cell death seen in Rbf^120a mutant eye discs. Co-expression of mir-998^Scer\UAS.cTa blocks this suppression.

The generation of Cbl^L.Scer\UAS mutant eye disc clones enhances the amount of cell death seen in Rbf^120a mutant eye discs. Co-expression of mir-998^Scer\UAS.cTa blocks this suppression.

(Truscott et al., 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

mir-998^Scer\UAS.cTa

mir-998^UAS.cTa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Truscott

Secondary FlyBase IDs

References (2)

Report Sections

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